• Title/Summary/Keyword: Marine National Park

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Haemocytes responses of the pearl oyster, Pincdata fucata, at different temperatures

  • Choi, Young-Joon;Hwang, Jee-Youn;Baeck, Gun-Wook;Kim, Mu-Chan;Park, Hyung-Jun;Choi, Byoung-Dae;Kang, Suk-Joong;Park, Chan-Il
    • Journal of fish pathology
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    • v.21 no.3
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    • pp.241-246
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    • 2008
  • The effects of temperatures on pearl oyster, Pincdata fucata were studied by evaluating some functional immune responses of the haemocytes. Water temperature is one of the most important factors in bivalve immune defense. Haemocytes comprise a primary line by inflammation, encapsulation and phagocytosis. These phagocytic abilities of haemocytes were observed in different temperatures. The number of the circulating haemocytes by migratory assay, phagocytic activities by MTT assay and reactive oxygen species production of haemocytes by CL assay were measured at different temperatures. Results showed that pearl oyster maintained at 20℃ and 25℃ displayed significantly higher values for all the measured immune parameters in comparison to maintained at 10, 15, and 30℃.

Preliminary EST analysis of immune-relevant genes from the liver of LPS-stimulated rock bream Oplegnathus fasciatus

  • Kim, Ju-Won;Park, Hyung-Jun;Baeck, Gun-Wook;Park, Chan-Il
    • Journal of fish pathology
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    • v.23 no.2
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    • pp.229-238
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    • 2010
  • We constructed a rock bream (Oplegnathus fasciatus) liver cDNA library and a total of 1533 expressed sequence tag (EST) clones were generated. Gene annotation procedures and homology searches of the sequenced ESTs were analyzed using BLASTX. Of the 1533 EST clones, 1165 different ESTs showed significant homology to previously described genes while 368 ESTs were unidentified, hypothetical, or unnamed proteins. Encoding 106 different sequences were identified as putative bio-defense genes or genes associated with immune response.

Molecular cloning and expression analysis of an interferon stimulated gene 15 from rock bream Oplegnathus fasciatus

  • Kim, Ju-Won;Kwon, Mun-Gyeong;Park, Myoung-Ae;Hwang, Jee-Youn;Park, Hyung-Jun;Baeck, Gun-Wook;Kim, Mu-Chan;Park, Chan-Il
    • Journal of fish pathology
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    • v.23 no.2
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    • pp.177-187
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    • 2010
  • The Interferon stimulated gene 15 (ISG15) is strongly induced in many cell types by IFNs, viral infections, and double-stranded RNA (poly I:C). The ISG15 homologue cDNA was isolated from the rock bream LPS stimulated leukocyte cDNA library. The rock bream ISG15 homologue was found to consist of 833 bp encoding 157 amino acid residues. Compared with other known ISG15 peptide sequences, the most conserved regions of the rock bream ISG15 peptide were found to be the tandem ubiquitin-like domains and a C-terminal LRLRGG conjugating motif, characteristic of mammalian and non-mammalian ISG15 proteins. Phylogenetic analysis based on the deduced amino acid sequence revealed a homologous relationship between the ISG15 sequence of rock bream and that of Atlantic salmon, Atlantic cod, northern snake head, black rockfish and olive flounder. The expression of the rock bream ISG15 molecule was induced in the peripheral blood leukocytes (PBLs) from 1 to 24 h following poly I:C stimulation, with a peak at 3 h post-stimulation. The rock bream ISG15 gene was predominantly expressed in the PBLs, spleen and gill.

Ecological Evaluation of Marine National Parks Based on Seaweed Community Index (해조류 군집지수에 기초한 해양국립공원의 생태학적 평가)

  • Oh, Ji Chul;Choi, Han Gil;Kim, Cheol Do;Ahn, Jung Kwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.49 no.3
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    • pp.385-392
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    • 2016
  • Seasonal variability in the algal community structure of intertidal zones was examined at six study sites in Marine National Parks, on the western and southern coasts of Korea from March to November 2015. A total of 193 species of algae, comprising 27 green, 40 brown and 126 red algae, were identified. Algal biomass averaged 101.50 g dry wt./m2 with a maximal at Tonggae (168.12 g dry wt./m2) and minimum at Haseom (54.49 g dry wt./m2). The dominant seaweeds by biomass were Sargassum thunbergii at five sites (Tonggae, Haseom, Namdongri, Ando amd Sinjeonri), and S. fusiforme at Soando. Community indices measured as follows: dominance index (DI), 0.31–0.69; richness index (R), 7.30–11.43; evenness index (J'), 0.33–0.49; and diversity index (H'), 1.15–1.86. As evaluated using community indices and environmental states, the EEI (Ecological Evaluation Index) of the Marine National Park was “normal”. The present results indicate that seaweed community structures and indices could be used to evaluate the environmental status of coastal ecosystems.