• Journal of Life Science
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• v.30 no.9
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• pp.783-790
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• 2020

The present study investigates the antioxidant and anti-inflammatory activities of Mangifera indica L. leaf extract. The total polyphenol content was measured using the Folin-Denis method. Results showed that the M. indica L. leaf extract of water and 70% ethanol showed a content of 440.83±1.02, 475.63±1.3 mg/100 g tannic acid equivalent. To assess antioxidant activity and electron-donating ability, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activity were measured, and all extracts were found to be highly efficacious. To assess cell viability of the extract from M. indica L. leaf on macrophage cells (RAW 264.7), a 3-[4,5-dimethyl-thiazol-2- yl]-2,5-diphenyl-tetrazolium-bromide assay was performed. The following experiments were conducted in section where cells was not shown of toxicity. In order to effectively determine anti-inflammatory activity, inhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 cells was examined using a Griess assay. The result showed that M. indica L. leaf extract concentration-dependently inhibited NO production. M. indica L. leaf extract was measured using Western blot, reverse transcription- polymerase chain reaction (RT-PCR) that to find the production of pro-inflammatory factor on stimulated RAW 264.7 cells of LPS. According to the results of this study, the M. indica L. leaf extract showed excellent effectiveness in antioxidant and anti-inflammatory activity, thus confirming its usability as a natural material and a functional raw material for cosmetics.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.146-152
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• 2016

Due to complicate canopy structures of Irwin mangoes grown in greenhouses, it is difficult to determine their growth parameters accurately. Leaf area, leaf fresh weight, and leaf dry weight are widely used as indicators to diagnose the tree growth. Therefore, it is necessary to establish models that can non-destructively estimate these growth indicators. The objective of this study was to establish regression models to estimate leaf area, leaf fresh weight, and leaf dry weight of Irwin mangoes (Mangifera indica L. cv. Irwin) by using leaf length, leaf width, petiole length, and SPAD value. The input values of leaf length, leaf width, petiole length, and SPAD value of 6-year old Irwin mangoes were measured, and the corresponding output values of leaf area, leaf fresh weight, and leaf dry weight were also measured. After 14 models were selected among the existing models, coefficients of the models were estimated by regression analysis. Three models with higher $R^2$ and lower RMSE values selected. In validation the $R^2$ values for the selected models were 0.967, 0.743, and 0.567 in the leaf area, leaf fresh weight, and leaf dry weight models, respectively. It is concluded that this models will be helpful to conveniently diagnose the growth of the Irwin mango.

• Journal of Forest and Environmental Science
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• v.30 no.2
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• pp.208-217
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• 2014

Leaf litter is the main and quick source of organic matter and nutrient to the soil compared to other parts of litter. This study focused on the nutrients (N, P and K) leaching from leaf litter of Melia azadirachta, Azadirachta indica, Eucalyptus camaldulensis, Swietenia macrophylla, Mangifera indica, Zizyphus jujuba, Litchi chinensis, Albizia saman, Artocarpus heterophyllus, Acacia auriculiformis, Dalbergia sissoo and Khaya anthotheca as the common cropland agroforest tree species of Bangladesh. About (9 to 35) % of initial mass was lost, while Electric Conductivity (EC) and TDS (Total Dissolved Solid) of leaching water increased to (573 to 3,247) ${\mu}S/cm$ and (401 to 2,307) mg/l respectively after 192 hours of leaching process. Mass loss (%) of leaf litter, EC and TDS of leaching water showed significant (ANOVA, p<0.05) curvilinear relationship with leaching time. Initial concentration of NH4, PO4 and K in leaching water was found to increase significantly (p<0.05) up to 48/72 hours and then remained almost constant at later stages (48/72 to 192 hours). Mass loss of leaves; EC, TDS, $NH_4$, $PO_4$ and K in leaching water was varied also significantly (ANOVA, p<0.05) among the studied tree species. All the tree species showed similar pattern of nutrients (K>N>P) release during the leaching process. The highest $NH_4$ (4,097 ppm) and potassium (8,904 ppm) concentration was found for M. azadirachta while the highest $PO_4$ (1,331 ppm) concentration was found for E. camaldulensis in the leaching water. Among the studied tree species, M. azadirachta, A. indica, D. sissoo, E. camaldulensis and Z. jujuba was selected as the best tree species with respect to nutrient leaching.

• Advances in environmental research
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• v.3 no.4
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• pp.355-365
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• 2014

The present study has been performed on one year old tree saplings of Azadirachta indica (L.), Cassia siamea (L.), Dalbergia sissoo (Roxb.), Eucalyptus rostrata (L.), Mangifera indica (L.) and Schyzygium cumini (L.) in order to assess the effect of exposure of $SO_2-NO_2$, alone and combination of two gases. Tree saplings have been exposed to an average of $495{\mu}g\;m^{-3}$ $SO_2$ and $105{\mu}g\;m^{-3}$ $NO_2$ for 40 d at the rate of $4hd^{-1}$ during 10:00 am to 01:00 pm in OTC. Total chlorophyll, specific leaf area (SLA), nitrate reductase (NR) activity, foliar protein, free proline content and free amino acids (AAs) of foliage have been the plant parameters, taken into consideration to evaluate the effect of gaseous exposure. Exposure of two gases has caused reduction in total chlorophyll content (P < 0.05, 0.01). Physiological and biochemical process has been seemed to be altered noticeable due to the combined effect of $SO_2+NO_2$ followed by $SO_2$ alone (P < 0.05, 0.01). $NO_2$ mediated stress has produced, stimulatory and inhibitory responses in tree saplings. Results reveal that tree saplings have been attempted to absorb the $NO_2$ through N assimilation pathway. E. rostrata, C. siamea have been emerged as moderate tolerant to $SO_2$ mediated stress followed by A. indica. Response pattern of S. cumini, M. indica and D. sissoo set them as good indicators of $SO_2-NO_2$ exposure. Effects of two gases on tree saplings have been found to be synergistic.

• Natural Product Sciences
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• v.5 no.1
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• pp.33-38
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• 1999

Ethanolic extracts of different parts of 10 local traditional vegetables (ulam) (Amaranthus gangeticus, Jussiaea linifolia, Eugenia polyantha, Trapa incisa, Trichosanthes anquina, Mangifera indica, Pachyrrhirus erosus, Barringtonia mcarostachya, Carica papaya, and Coleus tuberosus) were screened for in vitro antitumor promoting activity using the inhibition test of Epstein-Barr virus (EBV) activation in Raji cells induced by phorbol 12-myristate 13-acetate and sodium-n-butyrate. All the extracts were found to have strong inhibition activity toward EBV-activation, except for leaf extract of T. anquina. The extracts were non-cytotoxic to the Raji cells except for the extracts of A. gangeticus (leaves), B. macrostachya (leaves), E. polyantha (young leaves), and J. linifolia (leaves) where the viability of the cells were decreased significantly.

• Journal of Bio-Environment Control
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• v.24 no.3
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• pp.161-166
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• 2015

To determine the adequate levels of light intensity and $CO_2$ concentration for mango grown in greenhouses, quantitative measurements of photosynthetic rates at various leaf positions in the tree are required. The objective of this study was to develop two-variable leaf photosynthetic models of Irwin mango (Mangifera indica L. cv. Irwin) using light intensity and $CO_2$ concentration at different leaf positions. Leaf photosynthetic rates at different positions (top, middle, and bottom) were measured by a leaf photosynthesis analyzer at light intensities (0, 50, 100, 200, 300, 400, 600, and $800{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) with $CO_2$ concentrations (100, 400, 800, 1200, and $1600{\mu}mol{\cdot}mol^{-1}$). The two-variable model consisted of the two leaf photosynthetic models expressed as negative exponential functions for light intensity and $CO_2$ concentrations, respectively. The photosynthetic rates of top leaves were saturated at a light intensity of $400{\mu}mol{\cdot}^{-2}{\cdot}s^{-1}$, while those of middle and bottom leaves saturated at $200{\mu}mol{\cdot}^{-2}{\cdot}s^{-1}$. The leaf photosynthetic rates did not reach the saturation point at a $CO_2$ concentration of $1600imolmol^{-1}$. In validation of the model, the estimated photosynthetic rates at top and bottom leaves showed better agreements with the measured ones than the middle leaves. It is expected that the optimal conditions of light intensity and $CO_2$ concentration can be determined for maximizing photosynthetic rates of Irwin mango grown in greenhouses by using the two-variable model.