• Mycobiology
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• v.45 no.3
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• pp.220-225
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• 2017

Ginseng root rot caused by Cylindrocarpon destructans is the most destructive disease of ginseng. Six different fungicides (thiophanate-methyl, benomyl, prochloraz, mancozeb, azoxystrobin, and iprodione) were selected to evaluate the inhibitory effect on the mycelial growth and conidial germination of C. destructans isolates. Benomyl and prochloraz were found to be the most effective fungicides in inhibiting mycelial growth of all tested isolates, showing 64.7% to 100% inhibition at a concentration of $10{\mu}g/mL$, whereas thiophanate-methyl was the least effective fungicide, showing less than 50% inhibition even at a higher concentration of $100{\mu}g/mL$. The tested fungicides exhibited less than 20% inhibition of conidium germination at concentrations of 0.01, 0.1, and $1{\mu}g/mL$. However, the inhibition effect of mancozeb on condium germination of C. destructans was significantly increased to 92% to 99% at a higher concentration of $100{\mu}g/mL$, while the others still showed no higher than 30% inhibition.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.5
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• pp.280-284
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• 2006

The toxic effects of 14 commercially available fungicides on the earthworm, Eisenia fetida (Savigny), were evaluated using surface sprayed soil, immersion and contact filter tests. At recommended dose, the earthworm mortality is 4% on mancozeb, carbendazim, propineb and captan in the surface sprayed soil test, 4% on mancozeb, 6% on propineb and 10% on captan in the immersion test, and no effect on all used chemicals in contact filter test.

• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.40-44
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• 2001

Pesticide actual usage in fruit crop cultivation was surveyed, and usage trends of individual pesticides were evaluated to provide data for the development of indicators of environmental impact. The amount of pesticide used for fruit crops indicated the order of fungicide>insecticide>herbicide unlike the case of paddy rice. The fungicide rate of total usage was 72% in apple cultivation. Top ranking fungicides used on fruits were Mancozeb, Propineb, Thiophanate-methyl and the main insecticides were Mancozeb, Propineb, Thiophanate-methyl. The usuage by formulation types showed the order of WP>EC>SL>SC>SP>WG. Pesticide usage (a.i.) per hectare by different fruits was citrus 48.6kg, apple 27.1kg, pear 18.6kg, persimmon 17.5kg, peach 11.3kg and grape 9.2kg. Comparison of pesticide usage (a.i.,kg/ha) in some fruit crops between Korea and USA indicated that more pesticides were used for citrus, pear and peach in USA than Korea while more pesticides were used in Korea than USA for apple.

• The Plant Pathology Journal
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• v.18 no.3
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• pp.147-151
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• 2002

Seven fungicides were compared for the control of downy mildew on midge gourd. All treatments had significantly lower rates of disease progress curves and disease severity levels than that of the control. The highest yields were obtained from crops treated with metalaxyl + mancozeb, fosetyl-Al, and chlorothalonil. These treatments also proved to be the most economical considering the treatment costs.

• Biomedical Science Letters
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• v.22 no.4
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• pp.199-206
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• 2016

Pesticides are widely used to prevent loss of agricultural production but extensive exposure can induce health problems to pesticide operators. This study was performed to evaluate the health risk of highly produced pesticides used in fruit growing farm land by comparison of estimated exposure level with AOEL using KO-POEM program. AOEL was driven based on NOAEL of each pesticide evaluated by JMPR, EFSA or KRDA. In calculation of exposure level, types of formulation, dilution factors, spraying duration and motor type and exposure protection device were allocated according to actual condition of use. Dermal absorption rate was differently applied among EFSA default values (25% or 75%), general default value (10%) or real test result values to know the plausibility of default values and safety of pesticide to operators in outline. Twenty pesticide ingredients (fungicides and insecticides) were produced more than 30 tons per year, which were mancozeb, chlorothalonil, imidaclopirid and etc in order. Dermal absorption rates obtained from studies were various from 0.07 to 81% but mostly under 10%. The estimated exposure levels showed big differences more than 10 times higher when using EFSA default rate and up to 5 times higher when using general rate of 10% comparing using rates of test results. Mancozeb, chlorthalonil, diazinon and chlorpyrifos presented still higher exposure level than AOEL even when using test absorption rate from study, which suggests that re-evaluation of AOEL or dermal exposure absorption rate or strict management are required for health protection of operators who use those four pesticides in farm land.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.1018-1026
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• 2020

Rosemary essential oil was evaluated for antifungal potentiality against six major ginseng pathogens: Sclerotinia sclerotiorum, Sclerotinia nivalis, Cylindrocarpon destructans, Alternaria panax, Botrytis cinerea, and Fusarium oxysporum. The in vitro fungicidal effects of two commonly used fungicides, namely mancozeb and fenhexamid, and the volatile organic compounds (VOCs) of Trichoderma koningiopsis T-403 on the mycelial growth were investigated. The results showed that rosemary essential oil is active against all of the pathogenic strains of ginseng root rot, whereas rosemary oil displayed high ability to inhibit the Sclerotinia spp. growth. The highest sensitivity was S. nivalis, with complete inhibition of growth at 0.1% v/v of rosemary oil, followed by Alternaria panax, which exhibited 100% inhibition at 0.3% v/v of the oil. Minimum inhibitory concentrations (MICs) of rosemary oil ranged from 0.1 % to 0.5 % (v/v). Chemical analysis using GC-MS showed the presence of thirty-two constituents within rosemary oil from R. officinals L. Camphore type is the most frequent sesquiterpene in rosemary oil composition. Mancozeb and fenhexamid showed their highest inhibition effect (45% and 30%, respectively) against A. panax. T. koningiopsis T-403 showed its highest inhibition effect (84%) against C. destructans isolate. This study may expedite the application of antifungal natural substances from rosemary and Trichoderma in the prevention and control of phytopathogenic strains in ginseng root infections.

• International Journal of Industrial Entomology and Biomaterials
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• v.20 no.1
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• pp.1-5
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• 2010

The studies have been conducted to control the soil borne fungal pathogens viz, Fusarium solani (Mart) Sacc. and Alternaria tenuissima the incitants of root rot and die-back diseases on mulberry stem cuttings planted in the mulberry nurseries and also in established mulberry gardens ten plant extracts with 10% concentration except Lantana camara (undiluted) were tested through poisoned food technique and four biofungicides were also screened by dual culture method under in vitro conditions. Plant extract of Prosopis juliflora showed the maximum inhibition on the mycelial growth (81.2% over A. tenuissima and 80.0% over F. solani) and followed by L. camara (66.7% over A. tenuissima and 68.9% over F. solani). Among the antagonists Pseudomonas fluorescens and Trichoderma viride showed maximum inhibition on the mycelial growth of both pathogenic fungi. The promising plant extracts (P. juliflora and L. camara) and antagonists (P. fluorescens and T. viride) were tested against both the pathogenic fungi under in vivo conditions along with the existing popular chemical Mancozeb. All the tested plant products and bio-fungicides showed inhibitory effect on both fungi. But the maximum survival percentage of mulberry cuttings was recorded in the treatment with T. viride (95% against F. solani and 90% against A. tenuisssima) followed by P. fluorescens (90% against both fungi) and T. harzianum (80% against F. solani and 85% against A. tenuisssima). Incase of the treatments with plant extracts and chemical fungicide the P. juliflora (60% against F. solani and 55% against A. tenuisssima) showed higher survival percentage and followed by L. camara (55% against F. solani and 50% against A. tenuisssima) and Mancozeb (55% against both fungi). In case of control only 10% of survival was recorded in F. solani inoculated cuttings and 15% survival in A. tenuissima inoculated cuttings.

• Korean journal of applied entomology
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• v.42 no.1
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• pp.85-89
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• 2003

Current status of pesticide applications in sweet persimmon orchards was investigated through the questionnaire and analysis of the pesticide application records from 62 growers in 2000 and 17 growers in 2001 in Jinju, Sacheon, Changwon, Changyoung, and Gimhae in Korea. Average yearly application was 7.6 times in 2000 and 7.7 times in 2001, concentrated in June and July. The kinds of fungicides and insecticides used were 36 and 34 items in 2000, and 22 and 23 items in 2001, respectively. Application of pesticides was mostly finished by mid-September. Fungicides such as mancozeb, thiophanate-methyl, and benomyl were most frequently used in 2000, and lime sulfur, benomyl and carbendazim in 2001. Insecticides such as deltamethrin, methidathion, and fenitrothion + fenvalerate were frequently used in both years. About one-half of the pesticides being used by the growers was not registered for persimmon in Korea.

• The Korean Journal of Mycology
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• v.14 no.1
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• pp.71-78
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• 1986

Of the 1,200 single spore isolates of Alternaria mali causing apple leaf spot and fruit decay, the mycelial colonies showed 76, 96 and 15% growth at 100,000 ${\mu}g/ml$ of captafol, chlorothalonil and folpet, respectively, and five and three percent growth at 10,000 ${\mu}g/ml$ of iprodione and polyoxin. These isolates showed cross-tolerance of mycelial growth, spore formation, or spore germination to the above five fungicides plus captan, garbenda, and thiophanate methyl. Captafol, mancozeb, polydong, and propineb did not show any cross-tolerance of spore germination since no spore was germinated at 100 ${\mu}g/ml$ which was much lower than the concentration used by apple growers. Benomyl, fenarimol, oxidong, and triademefon showed medium degree of cross-tolerance of the fungal growth, sporulation, and spore germination.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.164-169
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• 2004

In the course of our screening for biocontrol agent (BCA) against Streptomyces scabiei and S. turgidiscabies causing potato scab using 5,000 actinomtcete isolates, 9 antagonistic strains were selected as BCA candidates through in vitro and in vivo assay. An antagonistic strain, A020645 was highly resistant to some pesticides and antibiotics such as dazomet and mancozeb and showed high control value in vivo. Two bioactive compounds (compound A, B) were purified by anion exchange chromatography, solid phase (ODS) extraction, TLC and reverse phase HPLC. Their chemical structures are now thought to be nucleoside derivative as determined by $^1H-NMR$ data analysis. Their full chemical structures would be elucidated through $^{13}C-NMR$, HMQC and HMBC analyses. Further studies will be focused on fitness in soil and formulation of the BCA candidates.