• 제목/요약/키워드: Man-chul

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TaqMan 실시간 중합 효소 연쇄반응에 의한 살모넬라속의 검출 및 ompC 항원단백 유전자의 비교 (Detection of Salmonella spp. by TaqMan real-time PCR and comparison of nucleotide sequences of ompC gene among Salmonella)

  • 이영성;최경성;김명철;한재철;채준석
    • 대한수의학회지
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    • 제42권4호
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    • pp.513-522
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    • 2002
  • Antigenic ompC genes of S. gallinarum, S. pullorum and S. dublin were characterized among Salmonella spp. isolated from chickens and other animals to identify genetic variation. Salmonella ompC gene fragment (1,027 bp) was amplified by PCR and the amplicons were cloned for comparison of nucleotide sequences. The identity of the sequences between S. gallinarum and S. pullorum, S. gallinarum and S. dublin, S. pullorum and S. dublin was 99.8%, 97.6% and 97.8%, respectively. Also, we found that ompC has some diversity between S. gallinarum and S. pullorum, and other Salmonella spp. which may be useful to type the organisms. Similar to diagnosis in other organisms, the TaqMan PCR method can be applied to rapid and accurate diagnosis of salmonellosis in chickens and other animals. We designed PCR primers and TaqMan probe for flagellin gene (fliC) for detection of Salmonella spp. by TaqMan PCR. The TaqMan PCR method was 10,000 times more sensitive than conventional PCR.

양면 투시기를 이용한 상악 신경 블록과 신경 박동성 고주파술의 치료 경험 -증례 보고- (Biplane Fluoroscopy Guided Maxillary Nerve Block and Pulsed Radiofrequency Lesioning of the Mandibular Nerve -A report of two cases-)

  • 이은형;박상리;조주연;한선숙;이철중;이상철
    • The Korean Journal of Pain
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    • 제18권2호
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    • pp.279-283
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    • 2005
  • Biplane fluoroscopy is usually used in angiography. Biplane fluoroscopy gives a biplane image with high resolution during the performance of operations. Trigeminal nerve blocks are effective treatment modalities for trigeminal neuralgia, and maxillary nerve block is the most dangerous procedure among them. The anatomic structures can change after head and neck surgery, so the trigeminal nerve block procedures cannot be done so easily. We used biplane fluoroscopy in these difficult cases. Our first case was a 60-year-old man who had undergone maxillary nerve block. The second case was of a 64-year-old man who had pulsed radiofrequency lesioning of mandibular nerve performed after head and neck surgery. With biplane fluoroscopy, we got good results without any complications.

원자력발전소 건설현장의 철근콘크리트 공종 생산성 및 영향요인 분석 (Reinforced-Concrete Works Productivity and Influence Factor Analysis on Nuclear-Power-Plant Project)

  • 허영기;임진호;김경욱;안영철;오재훈
    • 한국건축시공학회지
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    • 제14권4호
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    • pp.314-321
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    • 2014
  • 특수 플랜트 건설은 타 건설사업보다 체계적인 원가 및 공정관리가 요구되지만 이를 위하 반드시 필요한 현장 생산성을 구체적으로 분석한 연구는 이루어지지 않고 있다. 본 연구에서는 국내에서 건설중인 특수 플랜트 현장의 철근콘크리트공종을 형틀, 철근, 콘크리트로 나누어 생산성을 측정하고 생산성에 영향을 미치는 요인을 분석하였다. 생산성 측정결과 형틀공종 $0.54(m^2/man{\cdot}time)$, 철근공종 $0.06(ton/man{\cdot}day)$, 콘크리트공종 $1.98(m^3/man{\cdot}time)$로 나타났다. 영향요인으로는 형틀공종은 요일과 야간적업 유무, 철근공종은 야간잡업 유무, 콘크리트공종은 야간작업 유무와 콘크리트 물량이 통계적으로 유의게 영향을 미치는 것으로 분석되었다.

남병철의 혼천의 연구 I (ON THE ARMILLARY SPHERE OF NAM BYONG-CHUL-I)

  • 이용삼;김상혁;남문현
    • 천문학회지
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    • 제34권1호
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    • pp.47-57
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    • 2001
  • In this paper we have reconstructed an armillary sphere based on the Method of an Armillary Sphere Making described in the Volume 1 of The Collection of Writings on the Scientific Instruments-Uigijipsol (儀器輯說, two volumes) edited in the 1850's by Nam Byong-Chul (南秉哲, 1817-1863) who was a famous Korean states-man-scientist. Nam achieved convenience and accuracy in the measurements of stellar positions in the manner of selective setting the equatorial, ecliptic and horizontal poles by adding a pole axis exchange ring called Jaigeukkwon (載極圈) between the Three Arrangers of Time and Four Displacements. We made use of 3-dimensional graphic software for modelling Nam's armillary sphere which consisted of five layers-eight rings. Results of simulation showed that the pole axis exchange ring functioned properly in setting the equatorial, ecliptic and horizontal coordinates simply by exchange of positions of specified holes on the ring. We ascertained that the invention of Jaigeukkwon solved inherent problems in the conventional Chinese armillary sphere in computation of real ecliptic coordinates. It was revealed that Nam Byong-Chul made great contributions in the East Asian history of armillary sphere making.

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휘트먼 시의 민주주의 전망 (Democratic vistas in Walt Whitman's poetry)

  • 양현철
    • 영어어문교육
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    • 제9권spc호
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    • pp.167-184
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    • 2003
  • This paper is to analyze how Walt Whitman developed the theme and structure of Leaves of Grass with his ideal of democratic vistas. Whitman established his identity as an inspired poet, having faith in the divinity of man based on transcendental belief. After being awakened to the transcendental truth, he practiced his own common world view--his democratic vistas. Whitman searched for the unity with nature and identified his self with "common man and his nation." The poetry expresses "cosmological and national ideology" dedicated to the creation of an ideal nation united in eternal freedom and peace. By portraying common cosmic and national theme in terms of his individual personality, he brought various paradoxical and controversial ideas into one thing, namely "democracy", fusing diversity into unity. As in the symbol of the grass, there is a unity in variety reflected by democracy in a cosmological and political compound. With the form of free verse, he could express his liberal unrestrained and mystical thoughts of democracy. This new form has been associated with the poet's strong consciousness of the need for modernization in his country. He willingly assumed "the role of prophet and public voice for American democrat" with the rolling catalogues and I-persona which formed a sense of the common man and common things of America. Whitman pioneered a democrat literature with simple and dynamic tone and style. He successively pursued the democratic vistas in his Leaves of Grass.

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인조광물섬유 제품 제조 및 취급 근로자의 공기중 섬유 노출 평가 및 노동부 노출기준 고찰 (Workers' Exposure to Airborne Fibers in the Man-made Mineral Fibers Producing and Using Industries)

  • 신용철;이광용
    • 한국산업보건학회지
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    • 제15권3호
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    • pp.221-231
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    • 2005
  • In this study, occupational exposures to man-made mineral fibers (MMMFs) including glass wool, rock wool, and continuous glass filament fibers were determined and evaluated on the American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value (TLV). A total of 171 personal samples collected from 4 glass wool fiber, 2 rock wool fibers, 4 continuous filament glass fiber products manufacturing and a glass fiber and rock wool insulations using industries, and determined respirable fibers concentrations using the National Institute for Occupational Safety and Health (NIOSH) Method 7400, "B counting rule. The fiber concentrations of samples from workers installing thermal insulations in a MMMF using industry showed the highest value: geometric mean (GM) = 0.73 f/cc and maximum = 2.9 f/cc, 70% of them were above the TLV, 1 f/cc. Workers' exposure level (GM= 0.032 f/cc) in the rock wool manufacturing industries was significantly higher than those of glass wool (GM=0.012 f/cc) and continuous filament glass fibers (GM=0.010 f/cc) manufacturing industries (p<0.01). No samples were more than the TLV in the MMMF manufacturing industries. There was a significant difference among companies in airborne fiber levels.

인조광물섬유 산업에서 발생된 공기중 섬유의 크기 분포 (Size Distribution of Airborne Fibers in Man-made Mineral Fiber Industries)

  • 신용철;이광용
    • 한국산업보건학회지
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    • 제15권3호
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    • pp.213-220
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    • 2005
  • Penetration and health effect of fibers was related with their diameters and length. The purpose of this study is to characterize and compare the diameter and length of airborne man-made mineral fibers(MMMF) or synthetic vitreous fibers in the related industries. The average fiber length of the continuous filament glass, rock wool, refractory ceramic, and glass wool fibers production industries approximately 27, 28, 35, $50-105{\mu}m$. Airborne glass fibers were longest in all the type of MMMFs. The average diameters of airborne fibers generated from refractory ceramic, rock wool, glass wool, continuous filament glass fibers production industries were approximately 1.0, 1.6, 1.5-4 and $10{\mu}m$, respectively. The percentages of respirable fibers(<$3{\mu}m$) were 94% for RCFs, 73% for rock wool fibers, 61.0% for glass fibers, and 1.6% for filament glass fibers. The length of glass fibers were the longest in all types of fibers, and length of the others were similar. The refractory ceramic fibers were smallest in diameters and highest in fraction of respirable fibers.

통합경영 시스템을 통한 PL 대응 방안 연구 (A Study on the PL Response by Integrated Management System)

  • 김광수;임갑출;정순석
    • 품질경영학회지
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    • 제39권1호
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    • pp.120-130
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    • 2011
  • The purpose of this study is to offer the integrated model of the product safety management system, ISO 9001 Quality Management System, ISO 14001 environmental management system and OHSAS 18001 Occupational Health and Safety Management System and to present its management plan. In order to cope with effectuation of the Product Liability, studies were conducted to construct an integrated system and to find strategies of companies.

NDVI를 이용한 유역규모의 증발산량 분포 추정 (Estimation of Areal Evapotranspiration using NDVI)

  • 신사철;김만식;황만하;맹승진;고익환
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2005년도 학술발표회 논문집
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    • pp.761-765
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    • 2005
  • 원격탐사 기법은 식생 및 토지 이용과 같은 지형조건과 관련된 증발산량을 산정하기 위한 하나의 수단으로 효과적으로 이용될 수 있다. 지표면에서 발생되는 증발산량을 지배하는 인자는 기온, 습도, 바람, 일사량 및 토양조건 등 매우 복잡하게 구성된다. 식생은 그 지점의 증발산량에 영향을 주고 있으며, 증발산량을 지배하는 복잡한 인자는 식생의 성장조건에 직접적으로 영향을 미친다. 결국 증발산량과 식생조건 사이에는 강한 상관관계가 성립할 수 있음을 예상할 수 있다. 비교적 넓은 지점에 대한 식생상태의 파악을 위해서는 NOAA/AVHRR 자료가 효과적으로 이용될 수 있으며, 이로부터 얻어지는 식생지수(NDVI)를 이용함으로서 증발산량과 NDVI 사이의 강한 상관관계를 생각할 수 있다. 입력자료로 이용되는 기상자료가 많을수록 자료의 획득 및 처리에 많은 시간이 요구되므로 본 연구에서는 기상자료 중 비교적 쉽고 정확한 값을 얻을 수 있는 기온자료만을 채택하여 분석 시의 번거로움을 최소화하였다. 본 연구에서는 위성자료와 기상자료 중 가장 획득이 용이한 기온자료를 조합하는 간편한 방법에 의한 실제증발산량 산정방법을 제안한다.

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