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Detection of Salmonella spp. by TaqMan real-time PCR and comparison of nucleotide sequences of ompC gene among Salmonella  

Lee, Young-Sung (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University)
Choi, Kyoung-Seong (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University)
Kim, Myeong-Chul (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University)
Han, Jae-Cheol (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University)
Chae, Joon-Seok (Bio-Safety Research institute, College of Veterinary Medicine, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Research / v.42, no.4, 2002 , pp. 513-522 More about this Journal
Abstract
Antigenic ompC genes of S. gallinarum, S. pullorum and S. dublin were characterized among Salmonella spp. isolated from chickens and other animals to identify genetic variation. Salmonella ompC gene fragment (1,027 bp) was amplified by PCR and the amplicons were cloned for comparison of nucleotide sequences. The identity of the sequences between S. gallinarum and S. pullorum, S. gallinarum and S. dublin, S. pullorum and S. dublin was 99.8%, 97.6% and 97.8%, respectively. Also, we found that ompC has some diversity between S. gallinarum and S. pullorum, and other Salmonella spp. which may be useful to type the organisms. Similar to diagnosis in other organisms, the TaqMan PCR method can be applied to rapid and accurate diagnosis of salmonellosis in chickens and other animals. We designed PCR primers and TaqMan probe for flagellin gene (fliC) for detection of Salmonella spp. by TaqMan PCR. The TaqMan PCR method was 10,000 times more sensitive than conventional PCR.
Keywords
Salmonella gallinarum; S. pullorum; TaqMan real-time PCR; ompC gene;
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  • Reference
1 Gast RK, Detecting infections of chickens with recent Salmonella pullorum isolates using standard serological methods. Poultry Sci, 76:17-23, 1997
2 Cast RK, Beard CW. Age-related changes in the persistence and pathogenicity of Salmonella typhimurium in chickens. Poultry Sci, 68:1454-1460, 1989
3 Barrow PA. Immunity to experimental fowl typhoid in chickens induced by a virulence plasmid-cured derivative of Salmonella gallinarum. Infect Immun, 58:2283-2288, 1990
4 Holt PS, Chaubal LH. Detection of motility and putative synthesis of flagellar proteins in Salmonella pullorum cultures. J Clin Microbiol, 35(4):1016-1020, 1997
5 Gast RK, Holt PS. Application of flagellar-based immuonoassays for serologic of salmonella pullorum infection in chickens. Avian Dis, 42:807-811, 1998
6 Itoh Y, Hirose K, Miyake M, et al. Amplification of rfbE. and fliC genes by polymerase chain reaction for identification and detection of Salmonella serovar enteritidis, dublin and gallinarum-pullorum. Microbiol Immunol, 41:791-794, 1997
7 Singh SP, Singh SR, Wlliams YU, et al. Antigenic determinants of the ompC porin from Salmonella typhimurium. Infect Immun, 63:4600-4605, 1995
8 Puente JL, Alvarez-Scherer V, Gosset G et al. Comparison analysis of the Salmonella typhi and Escherichia coli ompC genes, Gene, 83:197-206, 1989
9 Park MK., Choi KS., Kim MC., et al. Differential diagnosis of Salmonella gallinarum and S. pullorum using PCR-RFLP. J Vet Sci, 2(3):213-219, 2001
10 Sambrook J, Fritch EF, Molecular cloning. Although manual. 2nd ed, Cold Spring Harbor Laboratory Press. 1981;1.3-9.58.38. Bimboim HC, Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res, 7:1513-1523, 1979
11 김원용, 장영효, 박경윤 등. Polymerase chain reaction과 Southern hybridization을 이용한 Salmonella 속균의 신속한 검출. 대한수의학회지, 35:531-536, 1995
12 최경성, 박진호, 권오덕 등. 중합효소연쇄반응을 이용한 자돈에서 Salmonella typhimurium의 신속한 검출. 대한수의학회지, 38(4):763-770, 1998
13 박두희, 김원용, 김철중 등. Polymerase chain reaction에 의한 Salmonella 속균의 검출. 대한수의학회지, 34:115-125, 1994
14 Cohen ND, Neibergs HL, Wallis DE, et al. Genusspecific detection of salmonellae in equine feces by use of the polymerase chain reaction. Am J Vet Res, 55:1049-1054, 1994
15 Bratin K, Saha. Baohong Tian, et al. Quantitation of HIV-1 by real-time PCR with a unique fluorogenic probe. J Virol Methods, 93:33-42, 2001
16 Ma L, Bluyssen HA, De Raeymaeker M, et al. Rapid determination of adenoviral vector titers by quantitative real-time PCR. J Virol Methods, 93:181-188, 2001
17 Lobos SR, Mora GC. Alteration in the electrophoretic mobility of ompC due to variations in the ammonium persulfate concentration in sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Electrophoresis, 12:488-450, 1991
18 Ewing WH. Edward and Ewing's Identification of Enterobacterceae. Amsterdam Elsevier Biomedical press, 1986
19 Olsen JE, Skov MN, Christensen JP, et al. Genomic lineage of Salmonella enterica serotype Gallinarum. J Med Microbiol, 45:413-418, 1996
20 Barrow PA, Bumstead N. Resistance to Salmonella gallinarum, S. pullorum and S. enteritidis in inbred lines of chickens. Avian Dis, 37:189-193, 1993
21 Puente JL, Flores V, Femandez M, et al. Isolation of an ompC-like outer membrane protein gene from Salmonella typhi. Gene, 156:1-9, 1995
22 Corpet F. Multiple sequence alignment with huerachical clustering. Nucl Acids Res, 16:10881-10890, 1988
23 Smith HW, Tucker JF. Ihe virulence of salmonella strains for chickens; their excetion by infected chicken. J Hyg, 84:479-488, 1989
24 오강희, 최원필. 초생추 유래 Salmonella 속균의 생물학적 특성. 대학수의학회지, 34:501-510, 1994
25 Chen W, Martinez G, Mulchandani A. Malecular Beacons: A real-time polymerase chain reaction assay for detecting Salmonella. Anal Biochem, 280:166-172, 2000
26 Li J, Smith NH, Nelson K, et al. Evolutionary origin and radiation of avian-adapted on-motile Salmonellae. J Med Microbiol, 38:129-139, 1993
27 김기석, 이희석, 모인필. 국내 닭에서의 가금티푸스 (Fowl typhoid) 발생. 농업과학논문집, 37:544-549, 1995
28 Tuchili LM, Kodama H, Izumoto Y, et al. Detection of Salmonella gallinarum and S. typhimurium DNA in experimetally infected chicks by polymerase chain reaction. J Vet Med Sci, 57:59-63, 1995
29 안종삼, 정석찬, 김종만, 등. Salmonella A 및 D group의 특이적 검색을 위한 rfbS 염기서열 분석에 기초한 PCR 기법의 적용. 대한위생학회지, 31:155-163, 1996
30 Puente JL, Juarez D, Bobadilla M, et al. The Salmonella ompC gene : structure and use as a carrier for heterologous sequences. Gene, 156:1-9, 1995