• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.445-451
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• 2012

The thinning effects of lime sulfur either alone or combined with fish oil on two apple cultivars 'Fuji'/M.9 and 'Hongro'/M.9 were investigated during two consecutive seasons. Either 1% or 2% lime sulfur at full bloom decreased the amounts of both terminal and axillary fruits compared with unsprayed 'Fuji' control trees in the first year. However, 1% or 2% lime sulfur did not decrease terminal fruit set in the second year. Only, triple applications of 1% lime sulfur significantly reduced the number of terminal and axillary fruits in both years. Multiple application of 1% lime sulfur was the most effective in thinning of 'Hongro'. In the first year, double or triple applications of 1% lime sulfur was effective in thinning terminal or axillary fruit, but one time applications of 1% or 2% lime sulfur was not reduced axillary fruit in 'Hongro' apple trees. In the second season, all treatments reduced terminal or axillary fruit in 'Hongro' clusters, except single application of 1% lime sulfur. Fish oil did not always improve the thinning effects of lime sulfur in 'Fuji' or 'Hongro' apple trees. None of treatments caused significant russeting in the harvested fruits. Most of thinning treatments had little effect on 'Fuji' or 'Hongro' fruit quality in terms of fruit weight, firmness, soluble solids concentration or titratable acidity.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.3
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• pp.100-110
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• 2009

'Hongro' is one of the important mid-season apple cultivars bred in Korea. The recent occurrence of water core in Hongro and the consequent problems motivated this study. The objectives of our study were to investigate the influence of air temperature during the growing period on water core occurrence in Hongro and to provide methods to mitigate its impact. Based on our field experiments for three years, the results indicated that the occurrence of water core disorder was due to the prolonged exposure to high temperature ($T_a$) of >$30^{\circ}C$ during the ripening period. The rates of occurrence of water core disorder were higher in the fruit whose weight was more than 300g or those located outside the tree canopy and thus exposed to stronger solar radiation. In terms of mitigating the water core occurrence, the application of spraying $CaCl_2$ four times from late July to August was effective. Furthermore, between 1 and 15 August when the rate of occurrence was high with $T_a$ (from 3:00 to 5:00 p.m.) >$30^{\circ}C$, the micro-water sprinkling for 30 minutes starting at 6:00 p.m. with a supplementary spray conducted two hours after the first application drastically reduced the water core occurrence.

• Korean Journal of Organic Agriculture
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• v.25 no.1
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• pp.125-134
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• 2017

This study was carried out to examine the effects of soil sulfur mixture on the control apple valsa canker in apple orchard for two years. In organic apple orchard, The recovery percentages of apple valsa canker were compared among control (no treatment), lime sulfur, and soil sulfur in organic orchards. In conventional apple orchards, those of recovery percentages were compared among control, neoasozin, lime sulfur, and soil sulfur. Compared with control, soil sulfur treatments significantly improved the recovery percentages of apple valsa canker infected trees in organic orchard. However, there were no differences between lime sulfur and soil sulfur treatment in organic apple orchard, except BongHyun orchard experiment in 2013. Compared with control fruit qualities, fruit skin red color and fruit firmness were improved in lime sulfur and soil sulfur treatment, respectively. In conventional orchard, apple trees treated with lime sulfur, soil sulfur or neoasozin improved recovery percentages, compared with those of control trees. Soil sulfur treatments recovered 87.5~97.5% of infected 'Fuji'/MM106 apple trees in organic and conventional apple orchards. The trees applied with neoasozin showed significant lower shoot growth than those of soil sulfur treatment in conventional orchard. Soli sulfur treatment improved fruit red color, but did not affected fruit weight, fruit firmness, soluble solids concentrations, and titratable acidity.

• Korean Journal of Food Science and Technology
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• v.40 no.2
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• pp.166-170
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• 2008

The effects of heat treatments on the antioxidant activities of selected fruits and vegetables were investigated by heating at various temperatures (110, 120, 130, 140, and 150$^{\circ}C$) for 2 hr. The examined fruits and vegetables included tomatoes (Lycopersicon esculentum), oriental melon (Cucumis melon var. makuwa), apples (Malus pumila Miller var. domestica Schneider), melon (Cucumis melon), watermelon (Citrullus vulgaris), and banana (Musa sapientum). The total polyphenol and total flavonoid contents of the juices from heated samples were quantified spectrophotometrically, and their antioxidant activities were determined using DPPH and ABTS radicals. As the heating temperature increased, antioxidant activity also increased. The highest total polyphenol content (2.80 mg/g) occurred in the oriental melon with heating at 150$^{\circ}C$, and this value was 7 times higher than that of the untreated oriental melon (0.40 mg/g). The highest total flavonoid content (148.80 ${\mu}$g/g) occurred in the melon heat treated at 150$^{\circ}C$, and this value was 37 times higher than that of the untreated melon (4.54 ${\mu}$g/g). DPPH radical-scavenging activity was the highest in the watermelon treated at 150$^{\circ}C$ (84.37%, 0.50 mg/g), and this value was 40 times higher than that of the untreated watermelon. Finally, the highest ascorbic acid (AA) equivalent antioxidant capacity (AEAC) value (239.50 mg AA eq/g) was obtained in the watermelon heat treated at 150$^{\circ}C$ for 2 hr (control = 18.35 mg AA eq/g).

• Horticultural Science & Technology
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• v.33 no.5
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• pp.784-788
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• 2015

A new cultivar 'Picnic' originated from an artificial cross between 'Fuji' and 'Sansa' at National Institute of Horticultural & Herbal Science in 1994. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2003. After regional adaptability tests in five districts for four subsequent years as 'Wonkyo Ga-34', it was ultimately selected in 2008. Optimum harvest time is late September. Mature fruit has mean weight of 233 g and is conic with light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains a favorable total soluble solids content at $13.8^{\circ}Brix$ and titratable acidity at 0.43%, which results in gustatory harmony between sugars and acids. It is not resistant to bitter rot or Marssonina blotch. 'Picnic' exhibits a physiological cross compatibility with leading cultivars such as 'Fuji', 'Hongro' and 'Tsugaru'. Tree topology is semi-spreading with a weak growth habit.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.352-357
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• 2019

In this study, we investigated the limit temperature range which is effective for dormancy release of 'Hongro' and 'Fuji' apples during the endodormancy period. The germination rate was 50% or more in all treatments except of 'Hongro' $-5^{\circ}C$ treatment. The germination rate of 'Fuji' was 86.3% at the temperature of $-5^{\circ}C$. The concentrations of carbohydrate and mineral components were significantly different between treatments but did not show any tendency or specific change. However, the sorbitol contents of 'Hongro' $-5^{\circ}C$ treatment were higher at 29.62 mg/g than those of 'Fuji', which was not affected for dormancy release. The results of hormone analysis showed that ABA(abscisic acid) and JA(jasmonic acid) in 'Hongro' $-5^{\circ}C$ treatment were respectively 176.48 and 15.72 ng/g. ABA, JA and SA(salicylic acid) contents in 'Fuji' apple tree were significantly lower than those of 'Hongro'. As a result of this study, the limit temperature range effective for dormancy release was different according to the varieties, and 'Hongro' did not affect with 30.3% of germination rate at $-5^{\circ}C$. However, 'Fuji' is 86.3% even at $-5^{\circ}C$ it was suggested that 'Fuji' had a chilling accumulation for dormancy release.

• Korean Journal of Plant Resources
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• v.32 no.5
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• pp.499-508
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• 2019

The study was carried out to investigate growth of 48.6-L pot-cultivated 1-year-old 'Fuji'/M.9 apple trees depending on different levels of nitrogen concentration. While rise in tree growth was paralleled with increase of nitrogen concentration, more than 32 mM of nitrogen rather restrained tree growth. In particular, growth of 16 mM of nitrogen treated trees was satisfied with criteria for production of high-quality pot-cultivated nursery stocks. Although mineral contents of leaves were higher in 8 and 16 mM nitrogen treatments than commonly recommended mineral contents in apple orchards, such somewhat surplus minerals could be helpful for tree growth after transplanting to apple orchards. In addition, our result indicated that soils of 8 and 16 mM of nitrogen treated pots met appropriate criteria for soil chemical property of apple orchards. Thus, in the light of tree growth, mineral contents of leaves, and soil chemical property in the pots, 16 mM of nitrogen treatment is considered to be suitable for production of 1-year-old 'Fuji'/M.9 apple potted trees.

• Journal of Bio-Environment Control
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• v.30 no.4
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• pp.342-350
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• 2021

This study was conducted to investigate the effect of time of hand-thinning on vegetative growth, bitter pit incidence, fruit quality, and return bloom in 'Gamhong'/M.9 apple trees. The time of hand-thinning were 3, 5, 7 and 9 weeks after full bloom, and the primary thinning (leaving only the king fruit on cluster) and secondary thinning (adjusting crop load) were conducted at the same time. The time of hand-thinning was correlated to the vegetative growth, average fruit wight, yield, soluble solids content, bitter pit incidence, and return bloom, negatively, and to the yield of middle grade fruits (fruit weight was 250-299g and none pit on fruit surface) per tree, calcium contents of leaves, and fruit red color, positively. There was no significant effect of time of hand-thinning on fruit firmness, titratable acidity, and total incomes per tree. In conclusion, if the time of hand-thinning of 'Gamhong'/M.9 apple tree was completed at 9 weeks after full bloom, it could produce about 300g of high-quality fruit without bitter pit.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.54-54
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• 2019

Apple (Malus domestica) is one of the most economically important fruits in Korea. But virus infection has decreased sustainable production of apple and caused the serious problems such as yield loss and poor fruit quality. Virus or viroid infection including Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV) and Apple scar skin viroid (ASSVd) has been also reported in Korea. In many cases, apple is infected with virus and viroid with no specific symptoms, the damage caused by the virus are unaware significantly. In our research, we tried to eliminate viruses in the rootstock for the disease-free seedlings of the apple dwarfing rootstock M.9 and M.26. The method of virus elimination was meristem culture, heat($37^{\circ}C$, 6weeks) treatment and chemistry($Ribavirin^{(R)}$) treatment. The analytical methods commonly used for the detection of virus is Enzyme-linked Immuno-Sorbent Assay(ELlSA) and Reverse Transcription-polymerase Chain Reaction(RT-PCR). RT-PCR method was more 30% sensitive than ELISA method. Efficiency of method eliminate virus appeared meristem method > heat treatment > chemistry treatment. The higher acquisition rate of disease-free seedlings is 30~40% on meristem treatment. In meristem treatment, the apple dwarfing rootstock M.9 gained infection ratio of ACLSV, ASPV and ASGV were 45%, 60% and 50% respectively. In the apple dwarfing rootstock M.26, infection ratio of ACLSV, ASPV and ASGV were 40%, 55%, 55%, respectively. Based on our results, it was found that most effective method of disease-free seedlings apple dwarfing rootstocks was by meristem treatment than heat method and chemistry treatment.

• Korean Journal of Plant Resources
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• v.34 no.1
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• pp.59-63
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• 2021

To study for germination rate of apple artificial seeds encapsulated with 2% alginic acids, the rates of germination for artificial seeds of various embryos with partial elimination of cotyledon measured by adding 80 mg/L gibberellic acid or/and nutritional media. The germination rate of artificial seeds produced embryo with 3/4 cotyledon (3/4 CE), embryo with 1/2 cotyledon (1/2 CE), embryo with 1/4 cotyledon (1/4 CE), and embryo without cotyledon (NCE) were gradually decreased with compare to normal embryo, but it's considerably recovered on the alginic acid containing MS solution or/and 80 mg/L GA3. In special, the low frequency (55.81%) of germination for artificial seeds of 1/2 cotyledon embryo with alginic acid was increased by treatment with combination of alginic acid, 3% sucrose, and 80 mg/L gibberellic acid (98.74%). In case of embryo without cotyledon, the low frequency of embryo without cotyledon (24.44%) in alginic acid almost recovered to 82.45% in alginic acid with MS solution and 80 mg/L gibberellic acid. The results shown that the germination of embryo was decreased by the removal of cotyledon, and demonstrated that the low rate of germination could be considerably increased by adding GA3 (80 mg/L) or/and nutrition.