Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Development of Artificial Seeds by Encapsulation Zygotic Embryo of Apple (Malus pumila var. domestica)

사과의 접합자배 encapsulation에 의한 인공종자 개발

  • Choi, Pil Son (Department of Oriental Pharmaceutical Development, Nambu University)
  • 최필선 (남부대학교 한방제약개발학과)
  • Received : 2020.06.04
  • Accepted : 2020.08.20
  • Published : 2021.02.01
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Abstract

To study for germination rate of apple artificial seeds encapsulated with 2% alginic acids, the rates of germination for artificial seeds of various embryos with partial elimination of cotyledon measured by adding 80 mg/L gibberellic acid or/and nutritional media. The germination rate of artificial seeds produced embryo with 3/4 cotyledon (3/4 CE), embryo with 1/2 cotyledon (1/2 CE), embryo with 1/4 cotyledon (1/4 CE), and embryo without cotyledon (NCE) were gradually decreased with compare to normal embryo, but it's considerably recovered on the alginic acid containing MS solution or/and 80 mg/L GA3. In special, the low frequency (55.81%) of germination for artificial seeds of 1/2 cotyledon embryo with alginic acid was increased by treatment with combination of alginic acid, 3% sucrose, and 80 mg/L gibberellic acid (98.74%). In case of embryo without cotyledon, the low frequency of embryo without cotyledon (24.44%) in alginic acid almost recovered to 82.45% in alginic acid with MS solution and 80 mg/L gibberellic acid. The results shown that the germination of embryo was decreased by the removal of cotyledon, and demonstrated that the low rate of germination could be considerably increased by adding GA3 (80 mg/L) or/and nutrition.

2% 알진산으로 캡슐화하여 만든 사과 인공종자의 발아 연구를 위하여 자엽을 부분적으로 제거한 후 영양분과 지베렐린을 단독 또는 조합처리한 알진산으로 캡슐화하여 발아율을 측정하였다. 3/4자엽 배(3/4 CE), 1/2자엽 배(1/2 CE), 1/4자엽 배(1/4 CE), 그리고 자엽을 완전히 제거한 배(NCE)로 만든 인공종자는 정상배로 만든 인공종자의 발아율 82.31%에 비하여 최대 24.44%까지 감소하였으며, 그 감소한 발아율은 MS영양분과 지베렐린 처리에 의해 상당히 회복되었다. 특히, 알진산 단독에서 1/2 자엽을 갖는 인공종자의 낮아진 발아율(55.81%)은 3% sucrose와 80 mg/L 지베렐린 조합처리에 의해 98.74%까지 증가하였다. 자엽이 없는 인공종자의 경우 낮아진 발아율(24.44%)은 MS용액과 80 mg/L 지베렐린 조합처리에서 82.45%까지 회복되었다. 이러한 결과는 배의 발아율이 자엽의 부분적 제거에 의해 억제되었고, 그 감소된 발아율은 영양분과 지베렐린 처리에 의해 상당히 회복될 수 있음을 보여 주었다.

Keywords

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