• Horticultural Science & Technology
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• v.17 no.4
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• pp.470-472
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• 1999

When the type and ratio of male-sterile plants in $F_1$ hybrids collected from several seed companies were investigated, there were differences in the male-sterile type depending upon region, seed company and variety group. The differences were inferred due to the easiness in breeding of maintainer line among the variety groups. American seed companies mainly used petaloid type male sterility with Imperater group varieties. European companies used brown anther type male sterility mainly with Nantes group but some companies used petaloid type also in varieties that were different from Nantes group. Asian companies (Japan, Korea) used both types with Chantaney and Kuroda group varieties, but one type was mainly used depending on individual seed company. Only one type of male-sterility in one variety was observed and the results were agreed well with other's results that male-sterility type was determined by cytoplasm factor. Some breeding lines were backcrossed to both types of cytoplasm (Sa, Sp) for maintainer line selection. We could select 15 maintainer lines from 20 lines in petaloid cytoplasm (Sp) and 3 from 4 lines in brown cytoplasm (Sa). In petaloid cytoplasm, maintainer lines can be selected at considerably high frequency. But in brown anther cytoplasm, the used materials are too restricted to tell general frequency maintainer lines.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.1
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• pp.53-57
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• 1990

Among the 6 treatment of not topped and suckered, topped and not suckered, hand suckered, maleic hydrazide treated, fatty alcohol treated to normal Hyangcho, and male sterile line, yield and Quality by price was lower at not topped and suckered, topped and not suckered or male sterile line plot. The content of petroleum ether extracts and ash was lower in hand suckered, maleic hydrazide and fatty alcohol plot, but the trends of reducing sugar, nicotine, and pH were in reverse. Reducing sugar was higher, nicotine and pH was lower in maleic hydrazide plot than in hand suckered or fatty alcohol plot. Filling value and combustibility was lower in maleic hydrazide plot than in hand suckered.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.30 no.4
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• pp.431-435
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• 1985

Nine hybrid rices crossed between Korean cytoplasmic-genetic male sterile rices having the WA cytoplasm and the Korean restorer lines or varieties, HR1619A/Nampungbyeo, HR1619A/Gayabyeo, HR1619A/Line234, TongilA/Nampungbyeo, TongilA/Cheongcheongbyeo, Suwon 296A/Line 209, Suwon 296A/Line 237, Suwon 296A/Line 252 and Line 201A/Line 234, and their parents were grown at Yeungnam University in 1984. The rough rice yield of the hybrids Line 201A/Line 234, TongilA/Nampungbyeo and HR1619A/Nampungbyeo were 939, 927 and 900 Kg/10a respectively. The heterosis(F$_1$/Midparent) of the above three hybrids was 40%, 20% and 19%, the heterobeltiosis(F$_1$/Better parent) was 36%, 17% and 10%, and the standard heterosis (F$_1$/Standard variety, Cheongcheongbyeo) was 19%, 17% and 14% respectively. The hybrids HR1619A/ Gayabyeo and Suwon 296A/Line 237 showed negative heterosis in grain yield. Significant heterobeltiosis for grain number per panicle was found while less or no heterobeltiosis was observed in panicle number per hill, 1000-grain weight and grain fertility. The bacterial leaf blight disease reaction of the hybrids tested was almost the same as that of one parent at least. The amylose content of the hybrids was medium to low the same as their parents. The protein content and alkali digestion value of the hybrids were almost the same as their parents.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.92-92
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• 2005

• Journal of agriculture & life science
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• v.52 no.6
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• pp.27-36
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• 2018

Recently, the demand for new cultivar of oriental mustard(Brassica juncea) is increased as the consumption of oriental mustard has increased dramatically in the market due to Kimchi is attracting the world's attention. However, absence of seed supply system can cause many problems including inbreeding depression due to self seed production, deterioration of the seed purity and heterogeneity of commercial seed. To establish the $F_1$ variety breeding system of oriental mustard, pure line and inbred lines were screened from inbreeding genetic resources. Male-sterile line was also selected from breeding combinations for the quality improvement of mustard. The combining ability from(Indojasai ${\times}$ Goheungdamyang) combinations and isolation line of(MS910 ${\times}$ Japan red mustard 8 ${\times}$ Ganghwa mustard 9) was highest, thus these lines were selected as parental lines. PCR(Polymerase Chain Reaction) and gene sequence analysis revealed that the genes related to CMS(orf263, orf220, and orf288) were distributed in mitochondria. The isolated lines from this study also showed good performance in yield test and farmhouse prove test.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.207-214
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• 1996

This study was conducted to obtain fundamental information on forage sorghum breeding in forage crop field of Livestock Experiment Station at Suwon from 1986 to 1991. The charcterization of sorghum germplasm was performed through 1986 to 1987, and after parental lines were selected from diverse sorghum germplasm on the basis of flowering date, plant height and several morphological characters for forage sorghum Fl hybrids. The range of variation of 50% flowering date and plant height were greater in order of forage sorghum sudangrass and male sterile line of grain sorghum. The average flowering date was earlier in sudangrass and male sterile line of grain sorghum than forage sorghum lines from the tested sorghum germplasms. And the average plant height was tall in order of forage sorghum, sudangrass and male sterile lines of grain sorghum. There were remarkable morphological variations between sudangrass lines and male sterile lines of grain sorghum such as plant color, leaf midrib color, glume color, seed coat color, head compactness and shape, awns, grain covering and 100 seed weight.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.180-183
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• 2011

'Yeomyeong', a new welsh onion (Allium fistulosum L.) variety, is developed by the Bioenergy Crop Research Center, NICS, RDA in 2004. This variety, as interspecific $F_1$ hybrid, is developed by a cross between onion (Allium. cepa L.) and welsh onion (A. fistulosum L). The first cross was conducted in 2003 between MOS8, onion male sterile line and G2, welsh onion inbred line. Horticultural and yield characteristics of this hybrid $F_1$ line was investigated in greenhouse for 2 years from 2004 to 2005 with fall cropping cultivation. It has a single pseudostem plant type, anthocyanin-colored pseudostem, and male sterile umbel. It showed intermediate plant type of the maternal parents in overall plant characteristics. 'Yeomyeong' is fall sawing variety and has higher plant height and pseudostem length than that of check variety 'Gumjang'. The yield potential of this variety was about 130.1MT/ha in greenhouse in spring harvseting season. This variety would be adaptable to the fall sowing cultivation in green house.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.6
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• pp.495-508
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• 1993

This experiment was conducted to investigate the amount of natural crossing at varying isolation distance and various transplanting time in tobacco. Normal variety and male sterile line showed an average of 1. 6 and 29% natural crossing in the adjacent plantings, respectively. The natural crossing was highest in the adjacent planting, as expected, and less at the farther distance from pollinator plants. Plants transplanted in April 20th were higher in natural crossing than those of other transplanting time.

• Journal of the Korean Society of Tobacco Science
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• v.22 no.1
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• pp.39-44
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• 2000

This study was carried out to obtain the basic information about the sucker producing characteristics of certain burley tobacco varieties(Nicotiana tabacum L. var. burley) at Suwon and Chonju Experiment Station, Korea Ginseng & Tobacco Research Institute in 1999. The number and weight of suckers for 12 varieties or breeding lines were investigated three times, 45 days after transplanting for ground sucker, 10 days after topping, and 10 days after maleic hydrazide(MH) applying for upstalk sucker. KB 103 and KB 9416-1 produced less ground suckers and upstalk suckers than other entries did. KB 9210-8 produced less ground suckers and (Male sterile TC 612 x KB 108)F$_1$ also produced less upstalk suckers than other entries did. There was no differences among entries in sucker production at 10 days after MH treatment. Between the weight of total sucker and days to flower revealed the negative correlation, but not significant. The low sucker producing entries described above could be used as a parental line in the breeding program for the low sucker producing variety.

• Molecules and Cells
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• v.25 no.1
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• pp.20-29
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• 2008

Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.