• The Transactions of the Korean Institute of Power Electronics
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• v.21 no.2
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• pp.175-181
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• 2016

In the conventional E-bike, a 42 V/10 A Li-ion battery drives a 24 V/10 A BLDC motor via a 6-switch PWM DC/AC inverter. The major problems of the conventional battery-fed motor drive systems are listed as follows. To charge the battery, an external battery charger (adapter) is required, which degrades the portability of E-bike users. In addition, given the high-frequency operation of the motor drive inverter, the switching losses are significant, which degrades the whole power efficiency. High-voltage batteries (42 V) require a complex battery management system (BMS), which degrades the reliability of the battery pack. In this paper, an embedded boost-converter battery charger for E-bikes is proposed. The variable output boost converter, which converts 16.8 V battery voltage to the required variable voltage of the inverter input, can use a low-voltage battery and thus improve the reliability of batteries. By varying the inverter input voltage via boost converter, a DC link voltage control method can be applied to reduce the switching frequency of the inverter, which improves the whole power efficiency. Given that the function of a flyback charger is integrated in the proposed boost converter, the portability of the E-bike user can be maximized by excluding an external adapter. The validity of the proposed circuit will be confirmed by operation mode analysis and simulation. Moreover, experimental results of integrative charger using Li-ion battery and 200 W motor test will be showed with a prototype sample as well.

• 한국균학회소식:학술대회논문집
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• 2016.05a
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• pp.27-27
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• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• Korean Journal of Environmental Agriculture
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• v.30 no.4
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• pp.453-458
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• 2011

BACKGROUND: Coleosporium plectranthi, an obligate parasite, which is responsible for the rust disease of Perilla frutescens, a plant in Korea, commonly known as Perilla. All rusts are obligate parasites, meaning that they require a living host to complete their life cycle. They generally do not kill the host plant but can severely reduce growth and yield. Food and feed spoilage fungi cause great economic losses worldwide. It is estimated that between 5 and 10% of the world food production is wasted due to fungal deterioration. Rust disease of Perilla is highly frequent and is widely spread in Korea. The present study was designed to investigate a novel media for the urediniospore germination in vitro and anti-rust activity as well as GC-MS analysis of oak pyroligneous liquor. METHOD AND RESULTS: Urediniospores were collected from the infected leaf of Perilla. Spore suspension was made and the suspension was inoculated in the 2% water agar media with proper humidity, then they were incubated at $26^{\circ}C$ for 56 hrs. The GC-MS analysis of the oak pyroligneous liquor was also done to check the chemical composition. GC-MS analysis of the wood vinegar was found 15 compounds, among them o-mthoxyphenol (25.93%), 2,6-dimethoxyphenol (16.06%), 4-methylenecyclohexanone (10.69%), 2,3-dihydroxytoluene (7.84%), levoglucosane (6.14%) and propanoic acid (5.32%) were the major components. Different concentration of the oak pyroligneous liquor was used, and spore inhibition was recorded on the basis of spore counting. The best results were noted at the concentration of 50% solution where 31.8% spores were inhibited. CONCLUSION: On the basis of the chemical composition of the oak pyroligneous liquor and the activity recorded we can use it as an anti-rust agent.

• Journal of the Korean Institute of Gas
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• v.24 no.4
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• pp.73-83
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• 2020

The problem addressed in this study is that the reduction in process accidents is less than the reduction in industrial safety, and the losses from major accidents in the oil and gas processing industry have not decreased over the years. In particular, current risk approaches in storage tanks place more emphasis on improving the safety of the design than maintaining safe operation. The Bow-Tie method can be used properly to improve process safety. The Bow-Tie method can assess compliance by focusing on operational aspects, clearly highlighting all important safety barriers, and analyzing barrier effectiveness.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.1
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• pp.99-105
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• 2009

The main purpose of this work is to analyse damages caused by debris flows during the heavy rainfalls at Inje of Kangwon Province, Korea. A series of site investigations have been carried out to survey the characteristics of debris flows occurred during the summer season of 2006. It has been found that major losses in human life and property are caused by discharge of soil and rock fragments from landslides. During the rainfall high precipitation rate of 113.5 mm/hour and 355 mm/day was recorded, which could happen at 80-500 year period. Comparing the rainfall record with the time of landslides being occurred, occurrence of the landslides is directly related to heavy rainfalls. At present, several debris barriers have been built at the valleys and natural slopes have been protected by the seed spray method. It is intended to propose an alternative of restoration of landslide damages and maintenance based on findings from the current study.

• Microbiology and Biotechnology Letters
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• v.49 no.3
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• pp.305-315
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• 2021

The cotton leafworm, Spodoptera littoralis, is a major pest in Egypt and many countries worldwide, and causes heavy economic losses. As a result, management measures to control the spread of the worm are required. S. littoralis nucleopolyhedrovirus (SpliNPV) is one of the most promising bioagents for the efficient control of insect pests. In this study, a chitinase gene (chitA) of a 1.8 kb DNA fragment was cloned and fully characterized from SpliNPV-EG1, an Egyptian isolate. A sequence of 601 amino acids was deduced when the gene was completely sequenced with a predicted molecular mass of 67 kDa for the preprotein. Transcriptional analyses using reverse transcription polymerase chain reaction (RT-PCR) revealed that chitA transcripts were detected first at 12 h post infection (hpi) and remained detectable until 168 hpi, suggesting their transcriptional regulation from a putative late promoter motif. In addition, quantitative analysis using quantitative RT-PCR showed a steady increase of 7.86-fold at 12 hpi in chitA transcription levels, which increased up to 71.4-fold at 120 hpi. An approximately 50 kDa protein fragment with chitinolytic activity was purified from ChitA-induced bacterial culture and detected by western blotting with an anti-recombinant SpliNPV chitinase antibody. Moreover, purification of the expressed ChitA recombinant protein showed in vitro growth inhibition of two different fungi species, Fusarium solani and F. oxysporum, confirming that the enzyme assembly and activity was correct. The results supported the potential role and application of the SpliNPV-ChitA protein as a synergistic agent in agricultural fungal and pest control programs.

• The Journal of Society for e-Business Studies
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• v.23 no.4
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• pp.41-62
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• 2018

Despite the rapid growth of the mobile shopping market, major market participants are continuing to suffer operating losses due to severe competition. To solve this problem, the mobile shopping market requires research to improve customer satisfaction and customer loyalty rather than excessive competition. However, the existing studies have limits to reflect the direct needs of customers because they extract the factors on the basis of the Technology Acceptance Model and the literature study. In this study, to reflect the direct requirements of users of mobile shopping Apps, we derived concretely and various factors influencing customer satisfaction through a topic analysis using user reviews. And then we assessed the importance of derived factors to customer satisfaction and analyzed the effects of customer satisfaction on customer complaints and customer loyalty on a structural equation model based on the American customer satisfaction index. We expect that our framework linking a topic analysis and a structural equation model is to be applicable to studies on the customer satisfaction of other mobile services.

• The Plant Pathology Journal
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• v.34 no.6
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• pp.544-554
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• 2018

Tomato (Solanum lycopersicum) production in Ghana is limited by the root-knot nematode (Meloidogyne incognita, and yield losses over 70% have been experienced in farmer fields. Major management strategies of the root-knot nematode (RKN), such as rotation and nematicide application, and crop rotation are either little efficient and harmful to environments, with high control cost, respectively. Therefore, this study aims to examine morphometric variations of RKN populations in Ghana, using principal component analysis (PCA), of which the information can be utilized for the development of tomato cultivars resistant to RKN. Ninety (90) second-stage juveniles (J2) and 16 adult males of M. incognita were morphometrically characterized. Six and five morphometric variables were measured for adult males and second-stage juveniles (J2) respectively. Morphological measurements showed differences among the adult males and second-stage juveniles (J2). A plot of PC1 and PC2 for M. incognita male populations showed clustering into three main groups. Populations from Asuosu and Afrancho (Group I) were more closely related compared to populations from Tuobodom and Vea (Group II). There was however a single nematode from Afrancho (AF4) that fell into Group III. Biplots for male populations indicate, body length, DEGO, greatest body width, and gubernaculum length serving as variables distinguishing Group 1 and Group 2 populations. These same groupings from the PCA were reflected in the dendogram generated using Agglomerative Hierarchical Clustering (AHC). This study provides the first report on morphometric characterisation of M. incognita male and juvenile populations in Ghana showing significant morphological variation.

• The Plant Pathology Journal
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• v.35 no.5
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• pp.425-436
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• 2019

Botrytis cinerea, a major phytopathogenic fungus, has been reported to infect more than 200 crop species worldwide, and it causes massive losses in yield. The aim of this study was to evaluate the inhibitory abilities and effects of Bacillus amyloliquefaciens RS-25, Bacillus licheniformis MG-4, Bacillus subtilis Z-14, and Bacillus subtilis Pnf-4 and their culture filtrates and extracts against the gray mold caused by B. cinerea on postharvest tomato, strawberry, and grapefruit. The results revealed that the cells of Z-14, culture filtrate of RS-25, and cells of Z-14 showed the strongest biocontrol activity against the gray mold on the strawberry, grape, and tomato fruit, respectively. All the strains produced volatile organic compounds (VOCs), and the VOCs of Pnf-4 displayed the highest inhibition values. Based on headspace solid-phase microextraction in combination with gas chromatography-mass spectrometry, esters accounted for the largest percentage of the VOCs produced by RS-25, MG-4, Z-14, and Pnf-4 (36.80%, 29.58%, 30.78%, and 36.26%, respectively). All the strains showed potent cellulase and protease activities, but no chitinase activity. RS-25, Z-14, and MG-4, but not Pnf-4, grew on chrome azurol S agar, and an orange halo was formed around the colonies. All the strains showed biofilm formation, fruit colonization, and lipopeptide production, which may be the main modes of action of the antagonists against B. cinerea on the fruit. This study provides the basis for developing natural biocontrol agents against the gray mold caused by B. cinerea on postharvest fruit.

• Journal of Industrial Convergence
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• v.14 no.2
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• pp.33-38
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• 2016

The Korean shipbuilding industry, which started in the 1970s with the advance of three shipbuilding companies, has been ranked as the world's largest and most successful model of the heavy and heavy chemical industry in the world since the 1990s, and has become a driving force for Korea's economic growth for several decades, including job creation and trade surplus. The domestic shipbuilding industry has won a lot of orders in favorable market environment, expanded facilities and manpower, built many ships and delivered them to shipowners, earning a lot of foreign currency and creating a 'successful myth.' However, when the global economic crisis broke out in 2008, shipbuilding in Chosun was stagnant and shipbuilding orders sharply decreased.As the facility and manpower increased in the boom period, the economy and the facilities become overcrowded as a result of the crisis, signs of a crisis in 2013 begin to appear. In 2015, three major Korean shipbuilders lost more than 6 trillion won in operating losses. Now, Korea's shipbuilding industry is facing a crisis such as massive insolvency and restructuring. Would not it have been possible to prevent the loss and restructuring of a trillion won if we recognized the recession of the global economy and understood the appropriate timing of technological innovation and prepared countermeasures against the crisis? Therefore, we analyze trends and trends of global shipbuilding industry such as Europe, China, and Japan in the competition structure of the shipbuilding industry and identify the problems of our shipbuilding industry and suggest suggestions.