• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제16권2호
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• pp.29-36
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• 2010

Recently, the system that can eliminate discrimination and barrier to disabled, ensure the substantial independence of disabled and help participation in community of disabled is required. Therefore, the purpose of this study is 'construction of the Barrier-free environment' which members of societies included the disabled can live safely in the daily living environment. To achieve this result, this study is shown establishment and complementary direction of the policy related convenient facilities by comparing between domestic and international policy of the convenient facilities. The results of this study are as follows; Firstly, among the conflicting part between related laws and design standards shold be constantly supplemented. Secondly, policy to promote convenient in residential part is needed. Thirdly, composition of the organization that can support professional housing reconstruction is required. Fourthly, from early stage of policy making, not only opinion reflection of users but also maintenance of convenient facilities, continuous maintenance plan and making a policy for verification system is needed. Finally, when the revision of the existing policies or new policies undergo, 'Barrier-free perspective' should be considered.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2016년도 춘계 학술논문 발표대회
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• pp.115-116
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• 2016

Numerous of free-form buildings come up with external appearances using various types of free-form panels. If the panel types produced, transport and installation order and maintenance history are not properly managed, it is difficult to complete a given project successfully. For free-form building projects that satisfy 5 factors (proper time, place, price, product and quantity), a supply chain management technique is applied for distribution management of free-form concrete panels. In addition, the study listed the whole production process of free-form concrete panels and any necessary information, and suggested a basic model for the management. The study result will be a great help in effective distribution management of free-form panels for free-form building projects.

• Parasites, Hosts and Diseases
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• 제36권2호
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• pp.99-108
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• 1998

베네수엘라분선충의 충란, 자충 띤 성충을 장기간 보존할 수 있는 방안을 마련하기 위하여 여러 가지 배양환경에 대한 연구를 수헝하였던 바, 다음과 같은 결과를 얻었다. 분변 내의 충란은 $4^{\circ}C$ 에서 25일간, 실온에서는 15일간 생존하였으며, 분변에서 분리한 충란은 $4^{\circ}C$의 생리적 식염수에 서 24시간 생존하였으나, 상실배기의 충란은 건조한 공기에 민감하여 12시간 내에 환성을 잃었다. 감염형 자충을 polyvinylbag에 넣어 $20^{\circ}C$에 보관하였을 경우, 분변 물질이 첨가된 0.12% 영양배 지에서는 45일, 영양배지는 28일간 생존하였다 한편, 영양배지에서 배양된 감염 자충은 상수에서 는 32일간, 멸균 식염수에서는 22일간 생존하였다. 인공감염시켜 얻은 성충은 $37^{\circ}C$에서 9일간, 9:1배지 (10% 쥐 혈청을 첨가)와 혈청을 첨가하지 않은 영양배지를 매일 교환하여 주어도 4일동안 밖에 생존하지 못하였다. 성충 암컷은 실험관 내에서 산란하고, 자충으로 부화되었지만 혈청을 첨가한 배지에서도 감염자충으로 발육하지 못하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제29권10호
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• pp.1398-1406
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• 2016

In general, the seminiferous tubule basement membrane (STBM), comprising laminin, collagen IV, perlecan, and entactin, plays an important role in self-renewal and spermatogenesis of spermatogonial stem cells (SSCs) in the testis. However, among the diverse extracellular matrix (ECM) proteins constituting the STBM, the mechanism by which each regulates SSC fate has yet to be revealed. Accordingly, we investigated the effects of various ECM proteins on the maintenance of the undifferentiated state of SSCs in pigs. First, an extracellular signaling-free culture system was optimized, and alkaline phosphatase (AP) activity and transcriptional regulation of SSC-specific genes were analyzed in porcine SSCs (pSSCs) cultured for 1, 3, and 5 days on non-, laminin- and collagen IV-coated Petri dishes in the optimized culture system. The microenvironment consisting of glial cell-derived neurotrophic factor (GDNF)-supplemented mouse embryonic stem cell culture medium (mESCCM) (GDNF-mESCCM) demonstrated the highest efficiency in the maintenance of AP activity. Moreover, under the established extracellular signaling-free microenvironment, effective maintenance of AP activity and SSC-specific gene expression was detected in pSSCs experiencing laminin-derived signaling. From these results, we believe that laminin can serve as an extracellular niche factor required for the in vitro maintenance of undifferentiated pSSCs in the establishment of the pSSC culture system.

• 한국구조물진단유지관리공학회 논문집
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• 제13권1호통권53호
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• pp.125-134
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• 2009

이 논문은 직시각형 단면을 갖는 원호형 등단면 띠기초의 자유진동에 관한 연구이다. 띠기초를 지지하는 지반을 두 변수 탄성지반으로 모형화하였다. 두 변수 탄성지반으로 지지된 원호형 띠기초의 휨-비틀림 자유 진동을 지배하는 미분방정식을 유도하고 이를 수치해석하여 고유진동수 및 진동형을 산정하였다. 띠기초의 경계조건은 자유-자유로 하여 최저저차 4개의 고유진동수를 산정하였다. 수치해석의 결과로, 중심각, 깊이비, 접촉비, 탄성계수비, 지반변수 등 5개의 변수가 고유진동수에 미치는 영향을 보고하였다. 변위 및 합응력의 진동형을 그림으로 나타내었다. 실험을 통하여 이 연구의 결과를 검증하였다.

• Journal of Veterinary Science
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• 제24권1호
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• pp.4.1-4.16
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• 2023

Background: In vitro culture of preantral follicles is a promising technology for fertility preservation. Objectives: This study aims to investigate an optimized three-dimensional (3D) fetal bovine serum (FBS)-free preantral follicle culture system having a simple and easy operation. Methods: The isolated follicles from mouse ovaries were randomly divided in an ultra-low attachment 96-well plates supplement with FBS or bovine serum albumin (BSA) culture or encapsulated with an alginate supplement with FBS or BSA culture. Meanwhile, estradiol (E₂) concentration was assessed through enzyme-linked immunosorbent assay of culture supernatants. The diameter of follicular growth was measured, and the lumen of the follicle was photographed. Spindle microtubules of oocytes were detected via immunofluorescence. The ability of oocytes to fertilize was assessed using in vitro fertilization. Results: The diameters were larger for the growing secondary follicles cultured in ultra-low attachment 96-well plates than in the alginate gel on days 6, 8, and 10 (p < 0.05). Meanwhile, the E2 concentration in the BSA-supplemented medium was significantly higher in the alginate gel than in the other three groups on days 6 and 8 (p < 0.05), and the oocytes in the FBS-free system could complete meiosis and fertilization in vitro. Conclusions: The present study furnishes insights into the mature oocytes obtained from the 3D culture of the preantral follicle by using ultra-low attachment 96-well plate with an FBS-free system in vitro and supports the clinical practices to achieve competent, mature oocytes for in vitro fertilization.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.351-354
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• 2005

Research purpose of this project is intending Maintenance Free of freight car and speed up of freight train. At goods transport as heightening traveling by ship speed of freight train shortening raise railway share of physical distribution and stand on tiptoe to Northeast Asia physical distribution main country to contribute expect. This paper is the performance elevation methods of next generation railway freight vehicles.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 춘계학술대회 논문집
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• pp.438-443
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• 2007

In the recent railway construction, the concrete slab track is highlighted as the maintenance-free track and the main stream is moving from ballasted track to concrete slab track. However, in spite of many merits of concrete slab track, the higher noise generated from the concrete slab track is a troublesome question to solve and, by this reason, many studies on noise reduction of concrete slab track are carried out. The railway noise can be classified into the reflection noise emitted from wheel/rail contact and the structure-borne noise transmitted through railway structures. In this presentation, we would like to introduce an example of the successful reduction of structure-borne noise at track retrofitting to maintenance-free concrete slab track in elevated Changdong Station which was built with ballasted track on Rahmen structure.

• 한국구조물진단유지관리공학회 논문집
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• 제7권4호
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• pp.171-181
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• 2003

비균열 무근 콘크리트에 매입된 단일앵커는 연단거리와 콘크리트 강도에 따라 콘크리트 단부파괴, 콘크리트 부서짐파괴, 앵커파괴가 발생한다. 본 연구에서는 연단거리와 콘크리트 강도에 따라 콘크리트 단부파괴, 콘크리트 부서짐파괴, 앵커파괴 발생시 단일앵커의 전단내력평가를 목적으로 한다. 이를 위하여 ACI 318-02와 EOTA 기준의 근거인 CCD (Concrete Capacity Design) 방법과 기존의 앵커 설계기준인 ACI 349-90(45-Degree Cone Method)에 의한 예측값과 실험값을 서로 비교 평가하였다.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2007년도 추계학술대회 논문집
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• pp.71-77
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• 2007

The surface of railway wheel tread in wheel-rail system can not be free from wear because of various circumstance such as railway condition, maintenance condition, weather condition, characteristics of wheel surface's geometrical shape, and vehicle suspension's structural characteristics etc. Therefore, the research on wear reduction and maintenance method are very important to ensure the safety of railway vehicle, to improve car comfort and to decrease maintenance cost. In this study, the wear rates of railway wheel have been periodically measured in terms of the running distance of Electrical Multiple Unit which have been currently operated and the microstructure transformation of wheel tread using replication method have been performed. The results show that the relations between the flange wear and tread microstructure are depended on running distance and it will supply basic data on wheel maintenance.