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Bioinformatic Analysis of NLS (Nuclear Localization Signals)-containing Proteins from Mollusks (생물정보학을 이용한 연체동물의 NLS (Nuclear Localization Signals) 포함 단백질의 분석)

  • Lee, Yong-Seok;Kang, Se-Won;Jo, Yong-Hun;Gwak, Heui-Chul;Chae, Sung-Hwa;Choi, Sang-Haeng;Ahn, In-Young;Park, Hong-Seog;Han, Yeon-Soo;Kho, Weon-Gyu
    • The Korean Journal of Malacology
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    • v.22 no.2
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    • pp.109-113
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    • 2006
  • Subcellular localization of a protein containing nuclear localization signals (NLS) has been well studied in many organisms ranging from invertebrates to vertebrates. However, no systematic analysis of NLS-containing proteins available from Mollusks has been reported. Here, we describe in silico screening of NLS-containing proteins using the mollusks database that contains 22,138 amino acids. To screen putative proteins with NLS-motif, we used both predict NLS and perl script. As a result, we have found 266 proteins containing NLS sequences which are about 1.2% out of the entire proteins. On the basis of KOG (The eukaryotic orthologous groups) analysis, we can't predict the precise functions of the NLS-containing proteins. However, we found out that these proteins belong to several types of proteins such as chromatin structure and dynamics, translation, ribosomal structure, biogenesis, and signal transduction mechanism. In addition, we have analysed these sequences based on the classes of mollusks. We could not find many from the species that are the main subjects of phylogenetic studies. In contrast, we noticed that cephalopods has the highest number of NLS-containing proteins. Thus, we have constructed mollusks NLS database and added these information and data to the mollusks database by constructing web interface. Taken together, these information will be very useful for those who are or will be studying NLS-containing proteins from mollusks.

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A Study on the Interpretalion of the Synthetic Unit Hydrograph According to the Characteristics of catchment Area and Runoff Routing (유역 특성과 유출추적에 의한 단위도 해석에 관한 고찰)

  • 서승덕
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.8 no.1
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    • pp.1088-1096
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    • 1966
  • The following is a method of synthetic unitgraph derivation based on the routing of a time area diagram through channel storage, studied by Clark-Jonstone and Laurenson. Unithy drograph (or unitgraph) is the hydrograph that would result from unit rainfall\ulcorner excess occuring uniformly with respect to both time and area over a catchment in unit time. By thus standarzing rainfall characteristics and ignoring loss, the unitgraph represents only the effects of catchment characteristics on the time distribution of runoff from a catchment The situation abten arises where it is desirable to derive a unitgraph for the design of dams, large bridge, and flood mitigation works such as levees, floodways and other flood control structures, and are also used in flood forecasting, and the necessary hydrologie records are not available. In such cases, if time and funds permit, it may be desirable to install the necessary raingauges, pruviometers, and stream gaging stations, and collect the necessary data over a period of years. On the otherhand, this procedure may be found either uneconomic or impossible on the grounds of time required, and it then becomes necessary to synthesise a unitgraph from a knowledge of the physical charcteristics of the catchment. In the preparing the approach to the solution of the problem we must select a number of catchment characteristic(shape, stream pattern, surface slope, and stream slope, etc.), a number of parameters that will define the magnitude and shape of the unit graph (e.g. peak discharge, time to peak, and base length, etc.), evaluate the catch-ment characteristics and unitgraph parameters selected, for a number of catchments having adequate rainfall and stream data and obtain Correlations between the two classes of data, and assume the relationships derived in just above question apply to other, ungaged, Catchments in the same region and, knowing the physical characteritics of these catchments, substitute for them in the relation\ulcorner ships to determine the corresponding unitgraph parameters. This method described in this note, based on the routing of a time area diagram through channel storage, appears to provide a logical line of research and they allow a readier correlation of unitgraph parameters with catchment characteristics. The main disadvantage of this method appears to be the error in routing all elements of rainfall excess through the same amount of storage. evertheless, it should be noted that the synthetic unitgraph method is more accurate than the rational method since it takes account of the shape and tophography of the catchment, channel storage, and temporal variation of rainfall excess, all of which are neglected in rational method.

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A terms learning strategy for improving learned capabilities of the subject, industry introduction (공업 입문 교과의 학습력 제고를 위한 용어 학습 전략)

  • Lee, Youngmin
    • 대한공업교육학회지
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    • v.31 no.2
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    • pp.41-63
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    • 2006
  • The purpose of this study was to establish and apply 'term learning strategy' for learners whose basic skill, interest, and motivation for learning is lower, and to increase the learned capabilities of subjects' 'industry introduction', required for industrial technical high school students. Another purpose was to identify when the learning strategy established was applied, its affect on how well learners understood terms in a particular unit of material, and, after instruction presented in accordance with 'term learning strategy,' evaluate the ease with which subjects understood the teacher's explanation of the unit of subject, how well they understand the main concepts of the unit of subject, and to what extent the presentation interested and motivated the subjects. The researcher established 'term learning strategy' based on the principles of Garg's "Nine Steps for Events of Instruction." The study included 35 first grade participants purposely selected from 33 classes at 1155 students in K industrial technical highschool. Data was collected via tests and surveys. Results indicated that 'term learning strategy' facilitated thorough understanding of terms and concepts related to the unit of subject, as well as a better understanding of the teacher's explanation and increased interest and motivation in learning. In addition, the "term learning strategy" increased interaction among learners in the instruction setting. Finally, implications of this study and implementation recommendations for this instructional strategy were presented.

Study of the Applications of Introduction of Computer Engineering Class using PBL (PBL을 이용한 컴퓨터공학입문 수업의 실제적 적용에 관한 연구)

  • Lee, Keun-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.10
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    • pp.6303-6309
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    • 2014
  • In this thesis, PBL was applied to the subject for improving students' many skills that modern industrial society demands. Our engineering school developed PBL problems for PBL use, applied the problems to classes and confirmed the effectiveness of PBL. The study subjects were 63 freshman students in H University who took the 'Introduce of computer engineering'. We applied 5 PBL problems for 15 weeks. They wrote and submitted a reflective journal when they finished the every given PBL activity. In addition, they completed a class evaluation form after the activity of 5th PBL Problem ended. The study showed that the students experienced the effectiveness of PBL, such as the comprehension of the studied contents, the comprehension of the cooperative learning, authentic experience, creative problem-solving skills, presentation skills, communication ability, self-directed study ability and confidence. Some difficulties in gathering together and spending much time were also encountered. The students realized that the PBL learning activities were important methods because the students could develop into future intelligent engineers that modern industrial society demands through PBL learning activities. The main goal of an engineering school is to produce specialists with creative problem solving ability so that the effects of this study are quite promising for our engineering school.

Two-phase Search Algorithm for Web Services Composition Redundanty (잉여 없는 웹 서비스 조합을 위한 2단계 탐색 알고리즘)

  • Kim, Hyeon-Ji;Kwon, Joon-Ho;Lee, Dae-Wook;Lee, Suk-Ho
    • Journal of KIISE:Databases
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    • v.36 no.2
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    • pp.123-138
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    • 2009
  • In recent years, the web services composition search has become an issue of great interest. The web services composition search is the process of integrating individual web services to yield desired behavior. Through the web services composition search, more sophisticated functionalities can be provided. Current solutions can be classified into three main classes: forward chaining approach, backward chaining approach and two-phase approach. However one-way chaining approaches, such as forward chaining approach and backward chaining approach have limitations of searching irrelevant web services. And two-phase approach has limitations of including redundant web services. In this paper, we propose an unredundant web services composition search based on the two-phase algorithm. The algorithm consists of a forward phase and a backward phase. In the forward phase, the candidate web services participating composition will be found efficiently by searching the Link Index. In the backward phase, unredundant web services compositions will be generated from candidate web services by using the Token Manager. The experimental results show that our proposed algorithm is more efficient than one-way chaining approaches. The experimental results also show that our algorithm can provide more solutions than previous two-phase approach and is comparable to previous one in execution time.

The Historical Backdrop and Reproduction of the Image in the Film (영화 <셰익스피어 인 러브>에 나타난 시대적 배경과 영상의 재현 - 르네상스시대의 공연예술과 초기자본주의 사회상을 중심으로)

  • Oh, Se-jung
    • Cross-Cultural Studies
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    • v.30
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    • pp.7-29
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    • 2013
  • A movie which brought its material from a historical character or incidents in the past was produced by a story suggestion through a historical fact. It is because Shakespeare created a story based on a mythical element related with his life in the plot which was written from the script of the play and was on the show in the cinemas of London. It is an obvious fact that the historical drama of this movie was intentionally modified and the fictional story was added to episodes in order to create a dramatic effect. However, reflecting historical backgrounds and cultural aspects accurately through a historical study would also be an important factor. Therefore, the backgrounds and aspects presented in this movie are a kind of storytelling which was reconstructed as if a historian added his opinion to historical facts like a discourse. A historical background in was a story about Shakespeare who worked at the theater in London as a writer in 1593 the period of England Reneissance. The movie included the working and playwriting of Shakespeare who is a main character. This indicated not only the environment of the theater and literature during the reign of Queen Elizabeth I but also historical aspect in the early modern industrial society in England. This movie, that is, described that time as a recreation such as a cultural acceptance and an achievement of an initial capitalism in Renaissance in the life of characters. In particular, the factor of theaters flourishing during the Renaissance was because a newly emerging class, bourgeoisie, who held the capital emerging from a policy for middle class led to a box office hit through founding theaters and drama company and selling tickets and performing plays by themselves. Like this, the movie depicted the time led by plays to a industrialization. Moreover, Social aspects in the late 1500s were revealed in this movie through a depiction of the cinemas and the city of London. The depiction of the city of London reflected a social situation of an initial capitalism rapidly developed in trade and commerce. The social aspects such as conflicts between social classes based on getting richer and poorer, mammonism, a corrupted love between the male and the female, a immortality with growing brothels, religious and political conflicts with the foundation of the church in England were closely linked with characters' daily routine at that time in London and were reflected in this work overall. The reason why we highlight characters' job and custom like this in the movie is that these are ideationally inherent in a critical mind from people at that moment. The historical background and reproduction of the image depicted in the movie were focused on characters' daily routine and indicated the problem mentally and independently exposed in the form of initial capitalism.

A Community Characteristic on Benthic Macroinvertebrates and Correlation of Physicochemical Water Quality Factors in Stream of Gaya Mountain (가야산 수계 내 저서성 대형무척추동물의 군집특성과 이화학적 수질요인과의 상관관계)

  • Kim, Hyoung-Gon;Yoon, Chun-Sik;Cheong, Seon-Woo
    • Journal of Wetlands Research
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    • v.20 no.4
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    • pp.322-329
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    • 2018
  • In this study the community characteristics of benthic macroinvertebrates distributed in stream of Gaya Mountain were figured out and the correlation with the physicochemical water quality factors were calculated. During the study period, total 4 phyla, 6 classes, 13 orders, 36 families, 100 species of benthic macroinvertebrates were recorded. Ephemeroptera were the most dominant followed by Trichoptera, Plecoptera, Diptera, Non-insecta, Hemiptera, and Odonata. The overall dominant species was Cincticostella levanidovae by dominance rate in 17.72%, subdominant species was Hydatophylax nigrovittatus by dominance rate in 6.15%. The biotic indices showed the diversity index 5.17, richness index 12.44, dominant index 0.24 and evenness index 0.78, there is no absolutely dominant benthic macroinvertebrates in the stream of Gaya Mountain. We identified the correlation between the species number and individuals of main taxa and the physicochemical water quality factors. Ephemeroptera and Plecoptera showed a negative correlation with water temperature, but they represented a positive correlation with the dissolved oxygen. This results suggest that water temperature and dissolved oxygen are important physicochemical water quality factors affects the distribution of Ephemeroptera and Plecoptera, the indicators of clean water stream.

Analysis of Inequality of Public Transfer Income by Income Level (소득계층별 공적이전소득의 불평등 변화분석)

  • Lee, Yong-jae;Kim, Yong-mi
    • Journal of Digital Convergence
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    • v.16 no.12
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    • pp.77-86
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    • 2018
  • This study was carried out by using the concentration index calculation method from 1996 to 2016 by using the household trend survey data to confirm the difference of income transfer income and inequality in public transfer income. The main results are as follows. First, the public transfer income concentration index in 1996 was concentrated on the high income group with +0.2774, but since 2009, the concentration index has been negative (-), which has concentrated on the low income group. However, the effect of redistribution of income was small. Second, the average public transfer income of low - income households increased significantly while the number of high income earners decreased. It is gradually improving that public transfer income did not play a role in the improvement of income inequality. Third, public transfer income has been continuously increasing in all income classes, and the rate of increase is low in the low income class and slow in the high income class, so the public transfer income of the low income class is higher than that of the high income class. In sum, the inequality of public transfer income by income class in Korea is gradually improving, but it is not considered to be a level that can improve the inequality between income groups.

The Effects of Project Learning of Pre-service Teachers on Self Directed Learning Ability and Creative Personality (초등예비교사의 프로젝트 학습이 자기주도적 학습능력 및 창의적 인성에 미치는 효과)

  • Lee, Yong-seob
    • Journal of the Korean Society of Earth Science Education
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    • v.12 no.2
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    • pp.141-150
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    • 2019
  • The purpose of this study is to investigate the effect of the pre-service teacher's maker project learning on self -directed learning ability and creative personality. This study established an eight-week period of experimental treatment from March to April 2019, and the students who participated in the study formed a research group of 75 students in three of their advanced classes who are attending the second semester of B University of Education and taking courses in 'Teaching Research 1'. The experimental treatment of research groups was conducted by the process of producing creative outputs through training of manufacturers. The experimental group of the study group was a process of producing creative output through maker education. The theme was set up to create creative output by group, worked with the main tool, 3D pen, and utilized the recycling material. When the creative output is completed, it is announced by presenting the actual product in each group. The results of the study are as follows. First, project learning by pre-service teachers had an effect on self-directed learning ability. Second, project learning by pre-service teachers had an effect on creative personality. Third, pre-service teachers was interested in learning about the project and responded positively to the students' perception.

Comparative study of thermal gelation properties and molecular forces of actomyosin extracted from normal and pale, soft and exudative-like chicken breast meat

  • Li, Ke;Liu, Jun-Ya;Fu, Lei;Zhao, Ying-Ying;Bai, Yan-Hong
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.5
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    • pp.721-733
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    • 2019
  • Objective: The objectives of this study were to investigate the thermal gelation properties and molecular forces of actomyosin extracted from two classes of chicken breast meat qualities (normal and pale, soft and exudative [PSE]-like) during heating process to further improve the understanding of the variations of functional properties between normal and PSE-like chicken breast meat. Methods: Actomyosin was extracted from normal and PSE-like chicken breast meat and the gel strength, water-holding capacity (WHC), protein loss, particle size and distribution, dynamic rheology and protein thermal stability were determined, then turbidity, active sulfhydryl group contents, hydrophobicity and molecular forces during thermal-induced gelling formation were comparatively studied. Results: Sodium dodecyl sulphate-polyacrylamide gel electrophoresis showed that protein profiles of actomyosin extracted from normal and PSE-like meat were not significantly different (p>0.05). Compared with normal actomyosin, PSE-like actomyosin had lower gel strength, WHC, particle size, less protein content involved in thermal gelation forming (p<0.05), and reduced onset temperature ($T_o$), thermal transition temperature ($T_d$), storage modulus (G') and loss modulus (G"). The turbidity, reactive sulfhydryl group of PSE-like actomyosin were higher when heated from $40^{\circ}C$ to $60^{\circ}C$. Further heating to $80^{\circ}C$ had lower transition from reactive sulfhydryl group into a disulfide bond and surface hydrophobicity. Molecular forces showed that hydrophobic interaction was the main force for heat-induced gel formation while both ionic and hydrogen bonds were different significantly between normal and PSE-like actomyosin (p<0.05). Conclusion: These changes in chemical groups and inter-molecular bonds affected protein-protein interaction and protein-water interaction and contributed to the inferior thermal gelation properties of PSE-like meat.