• Title/Summary/Keyword: Macrolide

Search Result 174, Processing Time 0.025 seconds

Functional Role of $^{60}RR^{61}$ in 23S rRNA Methylation, Which is in N-Terminal End Region of ErmSF (ErmSF의 N-Terminal End Region에 존재하는 $^{60}RR^{61}$의 23S rRNA Methylation에서의 역할)

  • Jin, Hyung-Jong
    • Korean Journal of Microbiology
    • /
    • v.44 no.3
    • /
    • pp.193-198
    • /
    • 2008

  • ErmSF is one of the proteins which are produced by Streptomyces fradiae to avoid suicide by its autogenous macrolide antibiotic, tylosin and one of ERM proteins which are responsible for transferring the methyl group to $A_{2058}$ (Escherichia coli coordinate) in 23S rRNA, which reduces the affinity of MLS (macrolide-lincosamide-streptogramin B) antibiotics to 23S rRNA, thereby confers the antibiotic resistance on microorganisms ranging from antibiotic producers to pathogens. ErmSF contains an extra N-terminal end region (NTER), which is unique to ErmSF and 25% of amino acids of which is arginine known well to interact with RNA. Noticeably, arginine is concentrated in $^{58}RARR^{61}$ and functional role of each arginine in this motif was investigated through deletion and site-directed mutagenesis and the activity of mutant proteins in cell R60 and R61 was found to play an important role in enzyme activity through the study with deletion mutant up to R60 and R61. With the site-directed mutagenesis using deletion mutant of 1 to 59 (R60A, R61A, and RR60, 61AA), R60 was found more important than R61 but R61 was necessary for the proper activity of R60 and vice versa. And these amino acids were presumed to assume a secondary structure of $\alpha$-helix.

  • PDF KSCI

Development of TaqMan Probe-Based Real-Time PCR Method for erm(A), erm(B), and erm(C), Rapid Detection of Macrolide-Lincosamide-Streptogramin B Resistance Genes, from Clinical Isolates

  • Jung, Jae-Hyuk;Yoon, Eun-Jeong;Choi, Eung-Chil;Choi, Sung-Sook
    • Journal of Microbiology and Biotechnology
    • /
    • v.19 no.11
    • /
    • pp.1464-1469
    • /
    • 2009

  • To achieve more accurate and rapid detection of macrolide-lincosamide-streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.

  • https://doi.org/10.4014/jmb.0902.0062 인용 PDF KSCI

Identification of Streptomyces sp. Producing Antibiotics Against Phytopathogenic Fungi, and Its Structure

  • Kim, Jung-Han;Jeong, Do-Hyeon;Park, Ki-Duk;Kim, Sung-Han;Kim, Kyung-Rae;Choi, Sung-Won;Kim, Ji-Tae;Choi, Ki-Hyun
    • Journal of Microbiology and Biotechnology
    • /
    • v.14 no.1
    • /
    • pp.212-215
    • /
    • 2004

  • In order to develop a biocontrol agent that can effectively control Fusarium wilt on Cymbidium genus, the effectiveness of antagonistic microbes against the cause pathogen was screened. The selected microbe showed a broad spectrum of antifungal activity, and the culture broth of this microbe had better preventive effect on Fusarium wilt than the commercial chemical agent in the pot assay. This isolated strain, GBA-12, was identified as Streptomyces kasugaensis, and the antifungal substance was purified from a broth culture of GBA-12. This purified substance was identified as a polyene macrolide (YS-822A) that was newly discovered from Streptomyces kasugaensis, and it exhibited antifungal activity against several phytopathogenic fungi.

  • PDF KSCI