• 제목/요약/키워드: Macrolide

검색결과 174건 처리시간 0.029초

ErmSF의 N-Terminal End Region에 존재하는 $^{60}RR^{61}$의 23S rRNA Methylation에서의 역할 (Functional Role of $^{60}RR^{61}$ in 23S rRNA Methylation, Which is in N-Terminal End Region of ErmSF)

  • 진형종
    • 미생물학회지
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    • 제44권3호
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    • pp.193-198
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    • 2008

  • ErmSF는, 235 rRNA의 A2058에 methylation 시켜서 macrolide-lincosamide-streptogramin B ($MLS_B$)계 항생제의 부착을 저해함으로써 항생제의 활성을 억제하는 내성인자 단백질인 ERM 단백질의 하나로, 다른 ERM 단백질과는 달리 긴 N-terminal end region을 가지고 있고 이 부위의 25%를 arginine이 차지하고 있다. 특히 $^{58}RARR^{61}$ 부위에 arginine이 모여 있어서 여기에 존재하는 R의 역할을 알아보기 위해 1-57, 1-59 그리고 1-60과 1-61이 제거된 결손 변이 단백질을 대장균에 발현하고 그 활성을 성장곡선을 작성하여 알아보았다. 그 결과 R60과 R6l이 활성에 중요한 것으로 관찰되었다. 1-59의 아미노산이 결손 된 유전자를 사용하여 R60A, R61A와RR60, 61AA의 위치선정 치환 변이 단배질의 세포내 활성을 측정하여 본 결과 R60의 역할이 R6l보다 큰 것으로 관찰되었으며 이들의 활성에서의 역할은 상호보완적인 것으로 나타났다. 그리고 이 아미노산들이 2차 구조인 $\alpha$-helix의 일부일 것으로 추정되었다.

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Development of TaqMan Probe-Based Real-Time PCR Method for erm(A), erm(B), and erm(C), Rapid Detection of Macrolide-Lincosamide-Streptogramin B Resistance Genes, from Clinical Isolates

  • Jung, Jae-Hyuk;Yoon, Eun-Jeong;Choi, Eung-Chil;Choi, Sung-Sook
    • Journal of Microbiology and Biotechnology
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    • 제19권11호
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    • pp.1464-1469
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    • 2009

  • To achieve more accurate and rapid detection of macrolide-lincosamide-streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.

  • https://doi.org/10.4014/jmb.0902.0062 인용 PDF KSCI

Identification of Streptomyces sp. Producing Antibiotics Against Phytopathogenic Fungi, and Its Structure

  • Kim, Jung-Han;Jeong, Do-Hyeon;Park, Ki-Duk;Kim, Sung-Han;Kim, Kyung-Rae;Choi, Sung-Won;Kim, Ji-Tae;Choi, Ki-Hyun
    • Journal of Microbiology and Biotechnology
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    • 제14권1호
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    • pp.212-215
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    • 2004

  • In order to develop a biocontrol agent that can effectively control Fusarium wilt on Cymbidium genus, the effectiveness of antagonistic microbes against the cause pathogen was screened. The selected microbe showed a broad spectrum of antifungal activity, and the culture broth of this microbe had better preventive effect on Fusarium wilt than the commercial chemical agent in the pot assay. This isolated strain, GBA-12, was identified as Streptomyces kasugaensis, and the antifungal substance was purified from a broth culture of GBA-12. This purified substance was identified as a polyene macrolide (YS-822A) that was newly discovered from Streptomyces kasugaensis, and it exhibited antifungal activity against several phytopathogenic fungi.

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