• 한국수산과학회지
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• 제22권5호
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• pp.219-227
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• 1989

효율적이고 신속한 어육의 선도측정을 위하여 시중에 유통되고 있는 몇가지 주요어종을 선정하여 이들의 선도를 저장온도에 따른 전기전도도 (electric conductivity)를 교류용 Kohlraush bridge, Wien bridge를 사용하여 제작한 Conductivity meter로 측정하여 다음과 같은 결과를 얻었다. 1. 냉동어육의 해동후 부패초기의 electric conductivity는 $6,100\pm({\mu}u/cm)$이었고 비동결어육인 방어와 쥐노래미 및 고등어의 부패초기 electric conductivity는 $20^{\circ}C$에서 $5,100\~5,400({\mu}u/cm)$로 냉동육에 비하여 약 $1,000({\mu}u/m)$낮았다. 2. VBN과 electric conductivity는 정상관관계를 가지며 부패초기 electric conductivity값이 나타났을 때의 VBN은 $30\~35mg\%$였다. 3. 적색육어인 고등어는 백색육보다 사후 시간의 경과와 더불어 electric conductivity의 온도의존성이 크다는 것을 알았다. 4. 고등어육의 electric conductivity를 $15^{\circ}C(\pm1^{\circ}C),\;20^{\circ}C(\pm1^{\circ}C),\;30^{\circ}C(\pm1^{\circ}C)$에서 저장시간과 관계를 측정, 온도계수 $Q_{10}=2.34$를 얻어 실측값과 유사함을 확인하였다. 5. 어류의 선도를 측정하는데 10초 내외의 짧은 시간에 비교적 정확한 결과를 얻을 수 있고 어육의 선도변화에 영향을 주는 요인들을 포괄적으로 지시하는 휴대용 Electric conductivity meter는 그 효용성이 높을 것으로 기대된다.

• 한국수산과학회지
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• 제49권6호
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• pp.737-742
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• 2016

This study examined changes in physicochemical indicators [K-value, lipid peroxide, and thiobarbituric acid reactive substances (TBARS)] in mackerel muscle during refrigerated storage at $4^{\circ}C$. Analyses were conducted every 3 days for 15 days. First, we visually observed changes in the mackerel. After 3 days, there were rapid changes in color and a rotten smell developed. The K-value of mackerel muscle was 4.21, 19.00, 42.13, 51.71, 71.19, and 80.34% on days 0, 3, 6, 9, 12, and 15, respectively; lipid peroxide level also increased, to 5.34, 5.44, 6.59, 7.29, 8.32, and 8.71 nmol/mL, respectively. TBARS increased from day 0 to 6, but did not change significantly after day 6 (0.98, 1.74, 2.73, 2.09, 1.86, and 1.93 mg Eq. malondialdehyde (MDA)/kg, respectively). The changes in K-value and lipid peroxide level demonstrated a loss of value as fresh food after day 6. The freshness of the stored mackerel had decreased by day 6 and mackerel stored for 9 days was less marketable.

• 생명과학회지
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• 제15권5호
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• pp.743-748
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• 2005

Histamine은 적색육 어류의 histidine이 어육 중의 Morganella morganii, Hafnia alvei 및 Klebsiella pneumoniae와 같은 부패세균에 의해 탈탄산 되어 초기에 형성되는 것으로 allergy성 식중독을 일으킬 수 있다. 이는 적색육 어류인 고등어의 선도저하 시에 많이 생성된다. 그리고 부패 후기에는 histamine을 분해하는 세균도 존재하는 것으로 알려져 있다. 그러므로, histamine 식중독의 잠재력을 지닌 자반고등어로 인한 식중독 사고 예방과 그 위생 대책을 수립하는데 필요한 자료를 얻고자 자반고등어에서 histamine 분해능을 가진 균을 분리, 동정하였다. 시료는 대형마트에서 시판되는 상태로 구입하였다. 질소원과 탄소원으로써 histamine만을 첨가한 제한배지를 사용하여 histamine 분해능을 가진 균을 분리하였다. 그리고 Cram staining, oxidase, catalase, citrate, TSI test, $H_{2}S$ reaction 및 indole 생성 등의 기본적인 생화학적 동정시험을 거쳐 10종의 시험균주를 선택하였다. 이 균주들을 16SrRNA gene 염기서열 비교에 의한 계통발생학적 분석을 이용하여 동정 하였다. 그 결과, Pseudomonas putida strain RA2, Halomonas marina, Uncultured Arctic sea ice bacterium clone ARKXV1/2-136, Halomonas venusta, Psychrobacter sp. HS5323, Pseudemonas putida KT2440, Rhodococcus erythropolis, Klebsiella terrigena (Raoultella terrigena), Alteromonadaceae bacterium T1, Shewanella massilia의 10종이 모두 동정 되 었으며, 각각 $100\%,{\;}100\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}99\%,{\;}100\%,{\;}95\%,{\;}99\%,{\;}100\%$의 상동성을 보였다. Histamine분해능의 존재를 탁도측정법과 효소법에 의해 확인한 결과, 분리된 10종 모두의 histamine 분해능이 재확인 되었고, 그 중 Shewanella massilia가 최대의 histamine 분해능을 보이는 것으로 확인되었다. 이 결과로 자반고등어 시판 제품에는 다수의 histamine 분해 세균이 존재하는 것을 확인할 수 있었으며, 이 세균을 활용한다면 식품 내 존재하는 histamine을 효과적으로 분해할 수 있을 것이라 예상된다.

• 수산경영론집
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• 제50권3호
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• pp.17-29
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• 2019

This paper aims to find out the problems and improvement direction of quality control and hygiene management of fishery products in Korea. For this reason, we conducted a comparative analysis of quality control and hygiene management of fishing, landing and selling between Japan and Korea, taking mackerel as an example. Japan has established a systematic freshness management system from fishing to landing (production) areas and consumption areas. On the other hand, Korea is not fully lead-managed in the production area after fishing, and is distributed in a state where the quality of the product is deteriorated due to exposure to room temperature. Accordingly, a certain quality can not be secured at the final consumption stage, and sanitary problems occur. In order to improve the quality control and hygiene management of the fishery products, the following needs to be improved. First, we will improve the fishing level freshness management system. Second, we will improve the quality control and hygiene management of fish in the production area and wholesale stage. Third, we will promote the introduction of innovative sales and consignment sales systems at the production stage. Fourth, we will establish a consistent low temperature distribution system from the production area to the wholesale stage from a long-term perspective. Fifth, we will promote the development and education of manuals on fish quality and hygiene management.

• 한국포장학회지
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• 제20권3호
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• pp.91-96
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• 2014

모델 현장으로 야외 임시 생선 판매장을 설치하여 TTI가 부착된 고등어 및 명태의 판매 중 TTI의 색 변화와 생선품질을 모니터링하였다. 생선은 스티로폼 박스 위에 얼음을 깔고 그 위에 진열되었고, 주기적으로 얼음을 채워서 신선도를 유지시켰다. 일정 시간 간격마다 고등어, 명태, TTI를 채취하여 TTI의 색 변화와 냉장생선의 품질 정도를 측정하였다. 품질 인자로 VBN과 Pseudomonas spp.를 고등어와 명태 각각에 대하여 적용하였다. 부패 시점은 VBN과 Pseudomonas spp.이 특정 수준에 도달했을 때로 간주하였다. 그 결과 온도가 높은 날에는 생선이 부패되기 시작하였고 그 시점에서 TTI 색이 종료점(제품 특성에 따름)에 도달하였다. 따라서 생선의 부패 시점을 TTI의 색 변화를 관찰함으로써 가능함을 알 수 있었다.

• 한국식품조리과학회지
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• 제21권5호
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• pp.662-668
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• 2005

To investigate the quality retention method of salted and semi-dried mackerel fillets, pH, volatile basic nitrogen (VBN), trimethylamine (TMA) and total bacterial counts in the fillets that were UV treated for 1, 3, and 4 hr were periodically measured during 48 days of refrigerated storage. The moisture, crude protein, crude lipid, and ash contents were 45.46 %, 18.87 %, 33.75 %, and 2.99 %, respectively. The freshness as determined by pH measurement could be maintained up to 25 days for the control and 31 days for the UV-treated fillets. VBN contents were continually increased over the storage time. The control reached the initial putrid level after 21 days while the UV-treated fillets after $25{\sim}31$ days. A similar tendency was shown for the changes of trimethylamine TMA contents, although the period differed slightly and the increase of the UV treatment time could decrease the TMA contents. The changes on the total bacterial counts of the fillets were maintained under 105 CFU/g during 48days. Especially, due to the effective decrease of the microorganism count by UV treatment at the beginning of the storage, UV -treated fillets showed lower bacterial counts than control over the whole period. E. coli. and Listeria were not detected in any fillets. Combining the above data and the sensory changes, the storage period of the fillets could be prolonged from 15 days at present to 30 days by UV treatments.

• 수산경영론집
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• 제41권3호
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• pp.59-78
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• 2010

This paper tries to apply the asymmetrical price transmission(APT) behavior observed in the agricultural industry to supply chains of the domestic fishery industry by a statistical manner. The fore mentioned asymmetrical price transmission refers to when price movements in the later stage of the supply chain do not move in a normal or symmetrical manner corresponding to price movements in the earlier stage of the supply chain. Therefore, when the earlier stage price increase and the later stage price increases to a larger degree, it is called positive(+) asymmetry and the opposite behavior is called negative(-) asymmetry. The study examines the data from domestic producers of three fresh fish types, hairtail, mackerel, and cuttlefish, and tries to examine the price asymmetry between the producer or farm, wholesaler, and retail prices via an APT test utilizing unit root, cointegration, and error correction model. The study found, hairtail wholesale and retail pricing bas a negative asymmetric relationship while mackerel has a negative asymmetric pricing relationship at the producer and retail levels of the supply chain. In the case of cuttlefish, all levels of the supply chain showed negative asymmetrical behavior in the supply chain price transmission, meaning the earlier stage price changes are more rapidly and greatly inputted in the later stage of the supply chain pricing. We believe that the reason why the analysis results show negative price asymmetry is due to the uniqueness of fishery products having an important variable such as freshness. If price increases are greater and quicker than price decreases, then consumer demand, which is sensitive to price increases will decrease and subsequently result in the increase of inventory levels, reducing profits for retailers. Also, frozen hairtail, mackerel, and cuttlefish will act as substitute goods to fresh fishery products. Therefore, fresh fishery products have a high demand of price elasticity. When prices increase, demand quickly decreases. Therefore the profit of wholesalers and retailers to decrease, I think this is the main reason of APT in the supply chain of Korea' s fisheries industry.

• Journal of Microbiology and Biotechnology
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• 제32권6호
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• pp.808-815
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• 2022

In this study, gamma-irradiated mackerel (Scomber japonicus) meat was stored in a refrigerator for 20 days to examine the physicochemical changes related to fishy smell. The effect of gamma irradiation on the inhibition of the activity of crude urease extracted from Vibrio parahaemolyticus was also evaluated. Increased levels of trimethylamine (TMA) and volatile basic nitrogen (VBN) content, which are the main components causing fishy smell, were significantly reduced by day 20 of storage after gamma irradiation, indicating that freshness was maintained during storage. The ammonia nitrogen contents of 3, 7, 10, and 20 kGy gamma-irradiated groups were significantly decreased by 6.5, 15.2, 17.4, and 23.9%, respectively, compared to non-irradiated groups on day 20 of storage. In addition, urease activity decreased in a gamma irradiation intensity-dependent manner. Volatile organic compounds (VOCs) were measured during the storage of gamma-irradiated mackerel meat. The contents of ethanol, 2-butanone, 3-methylbutanal, and trans-2-pentenal, which are known to cause off-flavors due to spoilage of fish, were significantly reduced by day 20 of storage. Therefore, gamma irradiation can be considered useful for inhibiting urease activity and reducing fishy smell during fish storage.

• 수산해양기술연구
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• 제35권1호
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• pp.60-64
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• 1999

The objective of this study was to manufacture the sea water cooling apparatus for fish hold storage to extend the freshness of fish. Using the sea water cooling apparatus manufactured, it makes observations on the physicochemical and sensual properties of mackerel such as ATP and its related compounds, K-value, VBN, TMA, TBA, salt-concentration and skin color through storage. The results of this investigation showed that the values of physicochemical properties such as ATP and its related compounds, K-value, VBN, TMA, TBA increased gradually during storage in sea water cooling apparatus manufactured and the changes of skin color was inhibited also. From these results, it could be suggested that developed sea water cooling apparatus is effective in shelf-life extension of fishes.

• 한국식품영양과학회지
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• 제12권2호
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• pp.62-65
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• 1983

어육을 단기간 저장하는데 효율적인 저장방법으로 알려진 빙결점동결법을 조리식품에 적용하여 저장중의 품질안정성에 대하여 실험한 결과를 요약하면 다음과 같다. 빙결점동결법으로 저장한 구운 고등어는 지방의 산패면에서 $5^{\circ}C$ 및 $-20^{\circ}C$에서 저장한 것보다 유도기가 약간 연장되는 효과가 있었으며, pH와 VBN는 세가지 저장조건에 따른 큰 차이가 없었다. 저장중 pH는 약간 감소하는 반면 휘발성염기질소는 저장초기에 급증하고 저장 4일 이후에는 큰 변화가 없었다. 생균수는 빙결점동결법으로 저장한 것이 $5^{\circ}C$ 저장한 것보다 상당히 낮은 값을 나타내었으며, $-20^{\circ}C$에서 저장한 것과 비교하여 큰 차이가 없었다. 그리고 관능검사의 결과도 빙결점동결법으로 저장한 것은 $-20^{\circ}C$에서 저장한 것과 비교하여 큰 손색이 없었다.