• Nutrition Research and Practice
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• 제3권2호
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• pp.156-161
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• 2009

Hyperglycemia in the diabetic state increases oxidative stress and antioxidant therapy can be strongly correlated with decreased risks for diabetic complications. The purpose of this study is to determine antioxidant effect of garlic and aged black garlic in animal model of type 2 diabetes. The antioxidant activity of garlic and aged black garlic was measured as the activity in scavenging free radicals by the trolox equivalent antioxidant capacity (TEAC) assay. Three week-old db/db mice were fed AIN-93G diet or diet containing 5% freeze-dried garlic or aged black garlic for 7 weeks after 1 week of adaptation. Hepatic levels of lipid peroxides and activities of antioxidant enzymes were measured. TEAC values of garlic and aged black garlic were $13.3{\pm}0.5$ and $59.2{\pm}0.8{\mu}mol/g$ wet weight, respectively. Consumption of aged black garlic significantly decreased hepatic thiobarbituric acid reactive substances (TBARS) level compared with the garlic group which showed lower TBARS level than control group (p<0.05). Activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of garlic and aged black garlic group were significantly elevated compared to the control group. Catalase (CAT) activity of aged black garlic group was increased compared with the control group. These results show that aged black garlic exerts stronger antioxidant activity than garlic in vitro and in vivo, suggesting garlic and aged black garlic, to a greater extent, could be useful in preventing diabetic complications.

• Clinical and Experimental Reproductive Medicine
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• 제28권2호
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• pp.161-167
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• 2001

Objective: Granulocyte-macrophage colony stimulating factors known to be secreted in murine and human reproductive tract. The development of human, bovine and murine embryos could be promoted by addition of GM-CSF in culture medium. However, the pregnancy and implantation rate of embryos cultured in GM-CSF have not been evaluated. The aim of this study was to assess the effect of GM-CSF in embryo development, pregnancy and implantation rate. Methods: A total of 191 IVF cycles were divided into control and GM-CSF supplement group (control=96, GM-CSF=95). The embryos were cultured for three day with or without 2 ng/ml of recombinant human GM-CSF. The quality of embryo, developmental velocity, pregnancy and implantation rates were compared. Results: There was no difference in age, number of gonadotropin ampules used, number of oocytes and fertilization. The number of ICSI cycle was higher in GM-CSF group. In GM-CSF group, G-1 grade embryos were the highest in proportion (56.4%), while G-2 grade embryos were highest (44.3%) in control group. The developmental velocity of embryos were not different between GM-CSF and control group. The pregnancy and implantation rates were significantly higher in GM-CSF group than control (47.4% vs. 33.3%, 17.0% vs. 11.1% respectively). Conclusion: By adding GM-CSF in culture medium, the quality of embryo, pregnancy and implantation rate could be improved.

• Clinical and Experimental Reproductive Medicine
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• 제28권2호
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• pp.147-153
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• 2001

Objective: The present study was performed to investigate the efficiency of partial laser assisted hatching (p-LAH; lased 1/2 ZP width from ZP edge) on hatching of mouse blastocysts. Methods: We used non-contact $1.48{\mu}m$ diode laser (MTM, Switzland) to create a precise hole on zona pellucida. 2-cell embryos were collected from the mouse (ICR) oviduct at 48 hours after hCG administration. Collected 2-cell embryos were cultured in the P-1 medium supplemented with 0.4% BSA. For experiments, embryos at 8-cell stage were used after $20{\sim}22$ hours in culture. After conventional (c-LAH) or partial laser assisted hatching, the embryos were further cultured in P-1 medium supplemented with 0.4% BSA for 3 days. To compare efficiency of complete and partial laser assisted hatching, hatching rate, hatching time and blastocyst diameter and zona pellucida thickness at hatching time were investigated. Embryos were examined every 12 hours. Blastocyst diameter and zona pellucida thickness at hatching time were measured with an ocular micrometer. Results: Hatching rates of p-LAH group (84.2%) was significantly higher than that of control group (39.3%), but there was no difference between the p-LAH (84.2%) and c-LAH (91.2%). p-LAH group was hatched 12 hours earlier than control group, but hatched 12 hours later than c-LAH group. The diameter of blastocyst at hatching time of p-LAH group ($113.1{\pm}6.4{\mu}m$) was smaller than that of control group ($122.2{\pm}5.0{\mu}m$), but larger than that of c-LAH group ($102.2{\pm}2.7{\mu}m$). Zona pellucida thickness at hatching time of p-LAH group ($6.4{\pm}0.9{\mu}m$) was thicker than that of control group ($4.5{\pm}1.5{\mu}m$), but thinner than that of c-LAH group ($10.0{\pm}0.8{\mu}m$). Conclusion: These results suggest that p-LAH may maintains the cell arrangement of early embryos to ensure successful development and prevent precocious hatching of blastocyst when compare to c-LAH and conventional (acidic tyrode) AH. Thus, p-LAH may provide a valuable and effective AH technique for human ART program.

• Applied Biological Chemistry
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• 제48권1호
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• pp.77-81
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• 2005

GM 콩으로 제조된 두부와 비지 등 콩 가공제품들의 효율적인 모니터링을 위하여 GM 콩이 0%, 1%, 3%, 5%, 100% 함유된 두부와 비지를 제조하여 이들의 삽입유전자인 5-enolpyruvylshikimate-3-phosphate synthase(epsps)를 검출하기 위한 PCR 방법과 EPSPS 발현 단백질 검출을 위한 western blotting과 lateral flow strip을 사용하여 검출 감도를 비교하였다. PCR 결과 산물의 크기가 큰 600 bp의 증폭에서 두부의 경우에는 100% GM으로 제조된 두부에서만 확인이 가능하였고, 비지에서는 5%, 100%에서 확인이 되었다. 크기가 작은 123 bp가 생성되는 실험에서는 0%를 제외한 모든 두부와 비지에서 검출되었다. Western blot 결과는 100% 두부, 비지에서만 검출이 되었고, lateral flow strip test에서는 100% 비지에서만 검출되었다. 이러한 결과로부터 GM 콩 가공식품 검출은 면역학적 방법보다는 PCR이 더 높은 민감도가 나타내는 것을 확인하였고, 또한 가공식품에서 효율적인 GMO 검출을 위해서는 100 bp 정도의 작은 크기의 PCR 산물을 이용해야 민감한 검출 결과를 보여주는 것을 확인하였다.

• 한국농공학회지
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• 제10권1호
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• pp.1394-1408
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• 1968

The tidal discharge is defined as the quantity of water flowing through a certain cross-section per unit of time, in contrast to river discharges, tidal discharges change periodically in magnitude and direction. Thus the total volumes of water flowing into again out of the system-called flood volume and ebb volume, respectively, depend on both the tidal and the river discharges. To ditermine the tidal discharge and the flood and ebb volumes of the Yong-san river, the discharges were measured at spring, mean and neap tide and simultaneous gage reading were taken at Samhak-do, Lower Myo-do, Myongsan-ni and Naju. The general procedure for measuring the tidal discharges was as follows. First, several cross-sections were measured and one of them was chosen. First, several cross-sections were measured and one of them was chosen. Then verticals were serected in the chosen cross section. Because comparatively few verticals should be representative of the discharge distribution over the river profile, the selection was done in accordance with the somtimes irregular bottom profile. The velocities were measured with the same current meters. The observations which included water level readings were continued for a period of about 13 hours. The current direction meter, a pyramid shaped resistance body, suspend in the water on a thin wire. The bubble in a circular tilting level fixed to the wire indicates the direction of the current. Reading were taken at intervals of 1m for depths of 10m or less, and for depths over 10m at intervals of 2m, going downwards and upwards. The averages of the two velocities were used for the computation of the discharges. The discharges and the flood and ebb volumes were ditermined by a graphical method. The mean velocities, corrected for their direction when necesary, were ditermined for each time interval and each vertical, and these velocities were plotted against the time. The resulting curves show possible mistakes very clearly, and the effect of observation errors could be reduced. The corrected velocities read from the curve at half-hour intervals were multiplied by the depth at the virtical at the corresponding time. The discharges thus found were ploted against the position of the vertical in the transit and joined by a smooth curve, integration of the curve rendered the total discharges as they occurred of half-hour intervals. Plotting these total discharges against the time yeilded during the day. The flood and ebb volumes were obtained by integration of the total discharge curve.

• Current Research on Agriculture and Life Sciences
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• 제9권
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• pp.21-28
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• 1991

벼 완숙현미(完熟玄米)의 배(胚)로부터 유기(誘起)된 callus의 계대배양(繼代培養)에 영향(影響)을 미치는 계대배양(繼代培養) 회수(回數), 기간(期間) 및 배지내(培地內)에 첨가(添加)되는 생장조정제(生長調整劑)의 조성(組成) 등(等)과 같은 몇가지 요인(要因)에 대한 실험(實驗)을 수행(遂行)하여 얻어진 결과(結果)는 다음과 같다. 벼 callus의 계대배양(繼代培養)은 4주간격(週間隔)보다는 2주간격(週間隔)으로 배양(培養)하는 것이 효과적(效果的)이었고 식물체(植物體) 재분화율(再分化率)은 품종(品種)과 계대배양(繼代培養) 기간(期間)에 따라 크게 상이(相異)한 것으로 나타났다. "영덕(盈德)벼"의 경우는 2주간격(週間隔)으로 4회(回) 계대배양(繼代培養)된 callus에서 72.5%의 가장 높은 재분화율(再分化率)을 보였으며, 4주간격(週間隔) 2회(回) 계대배양(繼代培養)된 callus에서도 58.3%의 재분화율(再分化率)을 보였다. Callus유기(誘起) 배지(培地)에 CH(2g/1), MI(200mg/1), TH(2mg/1)등(等)을 첨가(添加)하면 callus생장량(生長量)도 많았고 식물체(植物體) 재분화율(再分化率)도 향상(向上)되었으며, 식물체(植物體) 재분화(再分化)에는 kinetin단용(單用)보다는 kinetin과 NAA를 혼용(混用)하는 것이 효과적(效果的)인 것으로 나타났다. Callus 계대배양(繼代培養)에 CH, MI, TH 등(等)을 첨가(添加)한 배지(培地)를 이용(利用)하면 embryogenic callus의 발생량(發生量) 증가(增加)하는 경향이었고 품종(品種)에 따라서는 식물체(植物體) 재분화율(再分化率)도 높게 유지(維持)되는 것으로 나타났다.

• 한국전통조경학회지
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• 제32권1호
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• pp.44-52
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• 2014

본 연구는 최근 10년 동안 고문헌을 제재로 한 전통경관연구의 경향을 파악하여, 고문헌자료 활용에 있어 전통경관연구의 방향성을 제시하기 위한 것이다. 전통경관연구의 경향분석을 위해 2002년부터 2012년까지의 한국전통조경학회지에 발표된 논문을 조사 분석하여 전통경관연구의 고문헌자료 활용을 고찰하였다. 고문헌을 통한 연구논문의 수량은 2009년까지 한해에 2~3편 정도인 것이 2010년부터 8편 이상으로 많아졌다. 고문헌자료의 활용경향은 국역본을 사용하거나 개인문집의 일부를 번역하여 사용하는 경우가 대부분이었다. 원문사용에 있어서도 간혹 번역문만으로 연구를 진행하여 원문번역에 대한 고증부분이 미진한 점도 나타났다. 결과에 의해 전통경관연구의 방향을 아래와 같이 제안해 볼 수 있다. 원문의 효율적인 이해나 검증을 위해 원문과 번역본을 같이 제시하고, 번역의 출처도 명기해주는 것이 필요하다. 현황을 기록한 기문인 경우에는 조경 및 경관의 전문성이 갖추어진 번역 역시 필요한 것으로 보인다. 고문헌 자료의 분석에 있어서도, 시문과 기문 등을 병행한 전통경관 분석은 풍부한 자료로 인해 경관의 위치 및 장소 추정이 용이하므로, 기문 등을 근거로 연구의 기초를 잡은 후, 보완적으로 시문을 활용하는 등의 연구접근도 필요한 것으로 보인다.

• Journal of Microbiology and Biotechnology
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• 제24권10호
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• pp.1413-1420
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• 2014

Phytate is an antinutritional factor that impacts the bioavailability of essential minerals such as $Ca^{2+}$, $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, and $Fe^{2+}$ by forming insoluble mineral-phytate salts. These insoluble mineral-phytate salts are hydrolyzed rarely by monogastric animals, because they lack the hydrolyzing phytases and thus excrete the majority of them. The ${\beta}$-propeller phytases (BPPs) hydrolyze these insoluble mineral-phytate salts efficiently. In this study, we cloned a novel BPP gene from a marine Pseudomonas sp. This Pseudomonas BPP gene (PsBPP) had low sequence identity with other known phytases and contained an extra internal repeat domain (residues 24-279) and a typical BPP domain (residues 280-634) at the C-terminus. Structure-based sequence alignment suggested that the N-terminal repeat domain did not possess the active-site residues, whereas the C-terminal BPP domain contained multiple calcium-binding sites, which provide a favorable electrostatic environment for substrate binding and catalytic activity. Thus, we overexpressed the BPP domain from Pseudomonas sp. to potentially hydrolyze insoluble mineral-phytate salts. Purified recombinant PsBPP required $Ca^{2+}$ or $Fe^{2+}$ for phytase activity, indicating that PsBPP hydrolyzes insoluble $Fe^{2+}$-phytate or $Ca^{2+}$-phytate salts. The optimal temperature and pH for the hydrolysis of $Ca^{2+}$-phytate by PsBPP were $50^{\circ}C$ and 6.0, respectively. Biochemical and kinetic studies clearly showed that PsBPP efficiently hydrolyzed $Ca^{2+}$-phytate salts and yielded myo-inositol 2,4,6-trisphosphate and three phosphate groups as final products. Finally, we showed that PsBPP was highly effective for hydrolyzing rice bran with high phytate content. Taken together, our results suggest that PsBPP has great potential in the animal feed industry for reducing phytates.

• 한국임상수의학회지
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• 제25권4호
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• pp.295-302
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• 2008

Two dogs were referred to Veterinary Medical Center, Chungbuk National University with the clinical signs of vomiting, diarrhea, anorexia, and jaundice. Both dogs were diagnosed as extrahepatic biliary tract obstruction(EHBO) with concurrent pancreatitis based on dilated common bile duct and hyperechoic or mixed-echoic pancreas in abdominal ultrasonographic examination and serum biochemical abnormalities, such as high serum bilirubin, cholesterol, and increased cholestatic enzyme activity. Percutaneous ultrasound-guided cholecytocentesis(PUCC) was performed to examine bile and decompress the gall bladder. After PUCC with medical therapy, both dogs were steadily improved clinical signs of jaundice and anorexia. Also, cholestatic enzyme activity and serum bilirubin concentration decreased. Any complications that have been described in previous studies, such as peritonitis resulting from bile leakage and hemorrhage, were not identified. It is assumed that percutaneous ultrasound guided cholecystocentesis may be an useful diagnostic and therapeutic tool in canine gallbladder disease and can be used easily and safely to gain bile for diagnosis of bacterial cholecytitis.

• Clinical and Experimental Reproductive Medicine
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• 제38권4호
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• pp.203-209
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• 2011

Objective: This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. Methods: Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divided into three groups. One group was maintained as fresh controls (n=42), one was frozen by slow freezing (n=43), and one was cooled by vitrification (n=43). After thawing or cooling, survival rates, development to blastocyst, and cell numbers and inner cell mass (ICM) cell numbers of blastocysts were compared with those of the control group. The expressions of eight genes ($Rbm3$, $Birc5$, $Sod1$, $Sod2$, $Cirbp$, $Caspase3$, $Trp53$, $Hsp70.1$) were examined by real time-quantitative polymerase chain reaction in the fresh and frozen-thawed embryos. Results: There were no significant differences in the slow freezing and vitrification groups' survival rate after thawing (88.4% vs. 88.4%), development to blastocyst (100% vs. 97.4%), cell numbers ($107.0{\pm}21.0$ vs. $115.0{\pm}19.7$), or ICM cell numbers of blastocysts ($11.3{\pm}5.2$ vs. $11.1{\pm}3.7$). Cell numbers of blastocysts were significantly ($p$ <0.05) lower in the frozen-thawed embryos than the fresh embryos. There were no significant differences in the slow freezing and the vitrification groups' expressions of the eight genes. The expressions of $CirbP$ and $Hsp70.1$ were higher in the frozen-thawed embryos than in the fresh embryos but there were no significant differences. Conclusion: These results suggest that there were no significant differences between embryos that underwent slow freezing and vitrification.