• Journal of Microbiology
• /
• 제42권1호
• /
• pp.20-24
• /
• 2004

Cloned myo-inositol-1-phosphate synthase (INOS) of Drosophila melanogaster was expressed in Escherichia coli, and purified using a His-affinity column. The purified INOS required NAD$\^$+/ for the conversion of glucose-6-phosphate to inositol-1-phosphate. The optimum pH for myo-inositol-1-phosphate synthase is 7.5, and the maximum activity was measured at 40$^{\circ}C$. The molecular weight of the native enzyme, as determined by gel filtration, was approximately M$\_$r/ 271,000${\pm}$15,000. A single subunit of approximately M$\_$r/ 62,000${\pm}$5,000 was detected upon SDS-polyacrylamide gel electrophoresis. The Michaelis ($K_{m}$) and dissociation constants for glucose-6-phosphate were 3.5 and 3.7 mM, whereas for the cofactor NAD$\^$+/ these were 0.42 and 0.4 mM, respectively.

• 환경위생공학
• /
• 제16권3호
• /
• pp.14-21
• /
• 2001

The effects of sodium molybdate(Mo) on phospholipid metabolism in sciatic nerve isolated from lead-intoxicated rat were investigated. Four weeks aged albino rats were divided into four groups : normal control group, lead(10 ppm)-treated group, Mo(1 mg/kg)-treated group, lead and Mo-treated group. As a result of the study, Mo significantly increased the rate of incorporation of $2-[^3H]$myo-inositol into polyphosphinositides in lead-intoxicated rat. Mo also increased the rate of phosphatidylcholine metabolism in normal and lead-intoxicated rats. However, Mo did not affect the metabolism of phosphatidylserine and phosphatidylethanola-mine in normal and lead-intoxicated status. These results suggest that Mo might improve lead-intoxicated status by principally enhancing the metabolism of myo-inositol-related phospholipids and by partly phosphatidylcholine in sciatic nerve.

• 한국환경과학회지
• /
• 제9권3호
• /
• pp.209-214
• /
• 2000

The preventive effects of sodium molybdate on the acute toxicity of lead were studied by investigating tissue accumulation of lead, changes of nerve conduction velocity and concentrations of metabolites related to function of sciatic nerve in rats treated with lead, sodium molybdate and both, respectively. In lead-intoxicated rat, the conduction velocity, myo-inositol concentration and $Na^{+}/K^{+}$ ATPase activity of sciatic nerve were decreased by about 33 %, 48 % and 58 %, respectively. However, sodium molybdate treatment significantly normalized the conduction velocity, $Na^{+}/K^{+}$ ATPase activity and myo-inositol concentration of sciatic nerve in lead-intoxicated rat. Also, sodium molybdate treatment decreased the contents of lead in blood and sciatic nerve through promotion of urinary excretion of lead. But sodium molybdate treatment did not affect the glucose concentration in sciatic nerve. These results suggest that sodium molybdate prevented peripheral neuropathy not only by reducing lead contents in sciatic nerve and blood, but also by enhancing $Na^{+}/K^{+}$ ATPase activity in sciatic nerve.

• 한국축산시설환경학회지
• /
• 제7권2호
• /
• pp.83-102
• /
• 2001

Phytic acid (myo-inositol hexaphosphate or IP6) is the major storage form of phosphorus in cereals and legumes, representing 18 to 88% of the total phosphorus. Phytate form of phosphorus is not readily utilized by monogastric animals and this result causes pollution problem by phosporus released in areas of intensive livestock production. The interaction between phytic acid and essential dietary minerals, protein, or vitamins is considered to be one of the primary factors limiting the nutritional values of cereals and legunes in monogastric animals. Attempts have been made to hydrolyze dietary phytic acid by phytases to improve the feed quality and to decrease the amount of phosphorus excreted by animals. Phytase(myo-inositol hexakisphosphate phosphohydrolase) hydrolyzes phytic acid to myo-inositol and phosphoric acid. Two types of phytases are known: 3-phytase (EC 3.1.3.8) and 6-phytase (EC 3.1.3.26), indicating the intial attack to the susceptable phosphoester bond. Because of its great industrial importance, there is ongoing interest in isolating new bacterial strains producing novel and efficient phytases.

• Journal of Multimedia Information System
• /
• 제5권2호
• /
• pp.115-120
• /
• 2018

Recently, ways for accurately measuring the three dimensional movements of hand are actively researched so as to utilize the measurement data for therapeutic and rehabilitation programs. This research paper aims to introduce a product called Myo Armband, a wearable device comprised of a 3-axis accelerometer, a 3 axis gyroscope, and electromyographic sensors. We compare Armband's performance with that of the Motion Capture System, which is known as a device for providing fairly accurate measurements for angular movements of objects. Dart throwing and wrist winding motions comprised movement scenarios. This paper also discusses one of Armband's advantages - portability, and suggests its potential as a substitute for previously used devices. Decent levels of measurement accuracy were obtained which were comparable to that of three dimensional measurement device.

• 한국미생물·생명공학회지
• /
• 제51권3호
• /
• pp.328-331
• /
• 2023

Genomic information of biotechnologically and industrially important microorganisms provides the basis for understanding their metabolic potential. Here, we report the draft genome sequence of the Neodothiora populina-like yeast strain JAF-11 capable of producing biosurfactant myo-inositol lipids. The draft genome contained genes associated with secondary metabolite biosynthesis, including transport and metabolism of lipids, which are a major component of fungal surfactants. Identification of myo-inositol and acyl chain synthesis genes in the draft genome corresponded to the specific biosurfactant produced by strain JAF-11. Further experimental studies could help to elucidate the genes responsible for the production of biosurfactant by strain JAF-11.

• Molecules and Cells
• /
• 제38권9호
• /
• pp.781-788
• /
• 2015

Mutations of MYO15A are generally known to cause severe to profound hearing loss throughout all frequencies. Here, we found two novel MYO15A mutations, c.3871C>T (p.L1291F) and c.5835T>G (p.Y1945X) in an affected individual carrying congenital profound sensorineural hearing loss (SNHL) through targeted resequencing of 134 known deafness genes. The variant, p.L1291F and p.Y1945X, resided in the myosin motor and IQ2 domains, respectively. The p.L1291F variant was predicted to affect the structure of the actin-binding site from three-dimensional protein modeling, thereby interfering with the correct interaction between actin and myosin. From the literature analysis, mutations in the N-terminal domain were more frequently associated with residual hearing at low frequencies than mutations in the other regions of this gene. Therefore we suggest a hypothetical genotype-phenotype correlation whereby MYO15A mutations that affect domains other than the N-terminal domain, lead to profound SNHL throughout all frequencies and mutations that affect the N-terminal domain, result in residual hearing at low frequencies. This genotype-phenotype correlation suggests that preservation of residual hearing during auditory rehabilitation like cochlear implantation should be intended for those who carry mutations in the N-terminal domain and that individuals with mutations elsewhere in MYO15A require early cochlear implantation to timely initiate speech development.

• Investigative Magnetic Resonance Imaging
• /
• 제13권2호
• /
• pp.117-126
• /
• 2009

목적: 수치해석적인 방법을 통해 핵자기공명 분광기법에서의 다중양자 필터를 디자인하는 방법을 소개하고 이를 뇌의 중요한 대사체인 myo-inositol의 생체 내 검출에 이용하였다. 대상 및 방법 : 이를 위해 우선적으로 myo-inositol의 분광학적 성질을 조사 한 후 다중양자 필터의 echo time, mixing time 그리고 세번째 $9^{\circ}$ 펄스의 flip angle과 offset frequency같은 시퀀스 파라미터들을 최적화 하였다. 최적화된 필터는 우선적으로 실험 팬텀에서 테스트 한 후 최종적으로 인간의 두뇌에서 그 성능을 검증하였다. 결과: 실험결과를 토대로, 본 연구에서 제안하는 다중양자 필터를 사용하여 신호의 순수도가 크게 개선된 타겟 대사체의 신호를 얻을 수 있음을 알 수 있다. 결론: 수치해석적인 방법을 통하여 핵자기공명 분광기법에서의 다중양자 필터를 디자인함으로써 특히, 복잡한 스핀계를 갖는 타겟대사체의 신호의 순수도를 크게 강화할 수 있다.

• 한국전통조경학회지
• /
• 제38권3호
• /
• pp.75-82
• /
• 2020

흥선대원왕(興宣大院王) 이하응은 고종의 아버지로 1863년 12월에 대원군으로 봉작(封爵)되었다. 1898년 2월 2일 흥선대원군이 훙서(薨逝)하면서 평소 즐겨 지내던 마포구 공덕동의 아소당에 예장(禮葬)하게 되었고, 1907년 8월 24일 대원왕으로 추봉되었다. 대원왕으로 추봉된 이후 아소당 묘소가 파주 대덕동으로 천봉되면서 묘소가 원(園)으로 승격하게 되었다. 원으로 승격된 원침공간에는 원의 격에 맞는 시설물들이 조성되었는데, 이를 통해 흥원의 원과 묘의 원침 공간 특성을 분석할 수 있었다. 또한 묘와 원의 공간 구성을 비교·분석하여 조선시대 원, 묘의 공간 구성 특성을 도출할 수 있었다. 『흥원천봉등록』 분석을 통해 도출된 연구 결과를 살펴보면, 마포구 아소당에 위치한 흥선대원군의 묘소는 흥선대원군이 흥선대원왕으로 추봉되며 인조의 천봉 전 능소인 파주 운천리 장릉으로 천봉되었고, 묘에서 원으로 묘소의 위계도 승격되었다. 또한 마포 아소당에서 사용된 점경물과 시설물들을 파주 운천리로 옮기면서 재사용하였고, 원의 위계에 맞게 비석, 석호, 침각, 제물고, 수복청, 홍살문, 우물 등을 새로이 조성하였다. 이러한 점경물과 시설물 조성의 차이로 볼 때 원과 묘의 공간 구성에 차이가 있음을 확인할 수 있었다.

• 미생물학회지
• /
• 제12권2호
• /
• pp.85-93
• /
• 1974

Four stereoisomers of inositol p-aminosalicylate, that is hexaris-O-(p-aminosalicylyl)-myo-inositol, hexaris-O-(p-aminosalicylyl)-muco-inositol were synthesized by p-aminosalicylyl chloride with myo-, epi-, scyllo-, and muco-inositol respectively. Their antibacterial activity was tested against human type tubercle bacilli $H_{37}$/Rv, in contrast to 1.25.gamma./ml of p-aminosalicylic acid used as control. Hexaris-O-(p-aminoslicylyl)-muco-inositol showed the strongest antibacterial action at 0.625.gamma./ml, the other compounds more or less active than p-aminosalicylic acid.