• Development and Reproduction
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• v.4 no.1
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• pp.45-52
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• 2000

Membrane type matrix metalloproteinases(MT-MMPs) have been suggested to play an important role during structural remodeling of various tissue. Expression patterns of MT1-MMP and MT2-MMP mRNAs were investigated in oocytes, embryos, ovary and oviduct of mouse during their differentiation or periovulatory period using RT-PCR technique. Both cDNA products of MT1- and MT2-MMP of immature oocytes were barely discernable with a minimum amount but the expressions were distinct in mature oocytes regardless that they were matured in vivo or in vitro. MT2-MMP was not expressed by 2-cell embryos but was expressed by 4-cell stage embryos. From the morula stage untill hatched blastocyst stage, embyos showed intesnse expression of MT2-MMP with a sudden increase at blastocyst stage. While mouse ovarian tissues showed both expression of MT1- and MT2-MMP, there was no stage-specific difference throughout the estrous cycle. Mouse oviducts also exhibit constant amount of both MT1- and MT2-MMP expressions throughout periovulatory period, i.e., before or after ovulation. These observations lead to suggest that the differential expressions of maternal MT1- and MT2-MMP during meiotic resumption of mouse oocytes and embryonic expression of MT2-MMP particularly at blastocyst stage might play a role in the differentiation of mouse oocytes and／or embryos. The precise function of MT1- and MT2-MMP with regards to their participation in the remodeling of ovarian and oviductal tissues remains in a question.

• Journal of Plant Biology
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• v.4 no.2
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• pp.33-40
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• 1961

The moss flora and it's geoecological study were made on Mt. Odae which is situated in the easterm part(37$^{\circ}$43'N. Lat., 128$^{\circ}$30' E. Long) of Korea. The total number ofmosses collected on Mt. Odae in the autumn of 1960 is 50 species, 1 varieties and 1 subspecies, belonging to 23 families. For climate index, Warmth index and Humidity index were calculaterd on Mt. Odae. From the result, it was known that the climate of Mt. Odae is Humid temperate climate and that it's vegetation zone is Deciduous broad-leaf forest zone. Moss Quotient of this mountain is 1.21 and it indicates that the plant climate of Mt. Odae is Hemicryptophytic climate. The moss flora of this mountain was discussed from the pnytogeographical point of view. The flora consists of the following elements: Holarctic element : 20 species (38.5%) North Pacific element: 1 (1.9%) Cosmopolian element: 6 (11.6%) Tropical element: 1 (1.9%) East Asiatic element: 12 (23.05%) Korean-Japan element: 12 (23.05%) Coefficient of closeness was also calculated between the moss flora of Mt. Odae and that of Mt. Sulak and Mt. Kwanak. It was known, therefore, that the flora relation between Mt. Odae and the Mt. Sulak was closer than that between Mt. Odae and Mt. Kwanak.

• Journal of Life Science
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• v.18 no.3
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• pp.422-425
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• 2008

Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a membrane-associated zinc-dependent endoproteinase involved in extracellular matrix remodeling. MT1-MMP hydrolyzes ECM proteins like collagen and is involved in cancer cell migration and metastasis. Caveolins are integral membrane proteins and play a role in formation of caveolae, specialized membrane microdomains involved in clathrin-independent endocytosis. Recombinant MT1-MMP was transiently expressed in PANC-1 cells. Cells expressing recombinant MT1-MMP were able to hydrolyze collagen and migrate on collagen coated trans-well. Both subjacent collagen degradation and the cell migration conferred by recombinant MT1-MMP were inhibited by co-transfection of plasmids containing caveolin-1 cDNA. The results support that MT1-MMP is localized in lipid raft of the membrane and MT1-MMP activities in invasive cells could be inhibited by caveolin.

• Journal of Aquaculture
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• v.8 no.3
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• pp.183-193
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• 1995

Blue tilapia, Oreochromis aureus swim-up fry were fed,0, 1, 10, or 60 ppm $17\alpha-methyltestosterone$(MT) for 30 days (sex reversal period). Fish that had been fed 0 ppm MT during the sex reversal period were subsequently fed 0 ppm MT (0-0), 10 ppm MT (0-10) or 60 ppm MT (0-60) for the next 57 days (post sex reversal period) ; fish that had been fed 60 ppm MT during the sex reversal period were subsequently fed 0 ppm MT (60-0), 10 ppm MT (60-10), or 60 ppm MT (60-60) during the post sex reversal period. One group was fed 1 ppm MT (1-1), while another group was fed 10 ppm MT (10-10) during the entire 87-day experiment. There was a significant inverse relationship between concentration of MT and percentage body protein and percentage body fat at the end of the sex reversal period, while there was a significant positive relationship between concentration of MT and percentage body moisture. MT treatment during the post sex reversal period had greater effects on body composition of males at the end of the post sex reversal period than did MT treatment during the sex reversal period. There was a significant inverse relationship between MT concentrations, during both the sex reversal and post sex reversal periods, and gonosomatic indices (GSI) in males. In females, there was a significant inverse relationship between MT concentrations during the post sex reversal period and GSI.

• Journal of Environmental Health Sciences
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• v.28 no.2
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• pp.149-160
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• 2002

Analysis of benthic macroinvertebrate community structure and biological estimation of water quality by the benthic macroinvertebrates from the Mt. Myungji(1267m), Mt. Kalbong(840m), Mt. Daegeum(704m) and Mt. Jubal(489m) region of Gapyung-gun, Kyunggi-do were investigated from April to November, 2001. Total taxa of benthic macroinvertebrates were 105 species, 38 families, 13 orders, 5 classes in 4 phyla. Aquatic insects were 33 species in ephemeroptera, 5 species in odonata, 13 species in plecoptera, 2 species in hemiptera, 1 species in megaloptera, 27 species in trichoptera, 3 species in coleoptera and 13 species in diptera, respectively. Non-insects were 1 species in platyhelminthes, 5 species in gastropoda, 1 species in oligochaeta, 1 species in crustacea, respectively. Ephemeroptera, trichoptera and plecoptera as indicators in clean water were very abundantly. Occurrence species at each survey area was 67 species at Mt. Myungji and 69 species at Mt. Kalbong, 48 species at Mt. Daege.um and 47 species at Mt. Jubal, respectively. Mean species diversity indices at Mt. Myungji, Mt. Kalbong, Mt. Daegeum and Mt. Jubal were 3.30, 3.33, 2.59 and 3.43, respectively. According to the saprobic system based on the species diversity indices of benthic macroinvertebrates, Mt. Myungji, Mt. Kalbong and Mt. Jubal are determined as Limnosaprobic area, respectively, but Mt. Daegeum is determined as $\beta$-mesosaprobic area.

• Journal of Oriental Neuropsychiatry
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• v.18 no.1
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• pp.15-36
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• 2007

Objective : This study was to investigate the effects of several herbs on the levels of MT1 and MT2 melatonin receptors Methods: It was investigated the effects of several herbs such as WEDL, WEZV, WEFO, WEOC on the levels of MT1 and MT2 melatonin receptors using C6 glial cell model. ${\beta}-estradiol$ treatment, as a positive control group, under non-cytotoxic condition. Results : 1. The water extracts of Dimocarpus long (WEDL) induced the levels of MT2 melatonin receptor expression in a concentration-dependent manner without altering the levels of MT1 melatonin receptor expression. 2. The treatment with the water extract of Zizyphus vulgaris (WEZV) induced the levels of MT1 melatonin receptor expression and the levels of MT2 melatonin receptor expression was not affected. 3. The levels of MT1 as well as MT2 melatonin receptor expression were markedly up-regulated in the water extract of Fossilia ossis (WEFO) and the water extract of Ostreae caro (WEOC)-treated C6 cells. 4. The combination treatment with WEDL and WEZV induced not only the levels of MT1 melatonin receptor expression but also MT2 melatonin receptor expression, but the synergic effects of the combination treatment with WEFO and WEOC were not detected in C6 cells. Conclusion : The study provides important new insights into the possible mechanisms on the regulation of melatonin receptor synthesis by WEDL, WEZV, WEFO and WEOC.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.10b
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• pp.60-64
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• 2000

This examination of vegetation is conducted from lune to Oct. 1999 through on the spot survey, centering on the habitat of Abies koreana around Imgeolryeong, langteomok, and Jeseokbong in Mt Jiri and Yeongsil path and Seongpanak path in Mt. Halla. Species composition tables of the Abies koreana community by altitude and slope in Mt. Jiri and Mt. Halla are made based on the community composition tables examined in quadrat. The Abies koreana-Saso quelpaertensis community in a west slope of Mt. Halla is found that Abies koreana of 4-6m in height forms subtree layer and that of 8-9m in height the tree layer and it reflects a difference between community structure by slope and main composition species. While the tree layer of Abies koreana community is 12-14 in height around the area of 1, 290-1, 560m above the sea of Imgeolryong and path from Baekmudong to Jangteomok, it is 8-12m in height in the area of 1, 680-1, 780m above the sea of path from Jangteomok to Jeseokbong. It means that community structure depends on area and attitude. This study finds out that commonly appearing composition species similar to coverage and presence are Lepisorus thunberianus, Quercus mongolica, Soidago virga-aurea var. asiatica, Ligularia fischeri, Carex lanceolata, Clintonia udensis, Magnolia sieboldii, Betula ermani, Acer Pseudo-sieboldianum, As soil environment of surveyed area, pH of surveyed spots is similar in Mt. Jiri and Mt. Halla, electrical conductvity is higher in Mt Jiri, and contents of organic matter is relatively higher in a west slope of Mt. Jiri and Mt. Halla and lower in their south and west slopes. Contents of organic matter and total nitrogen show that the area of 1, 550m above the sea in a west slope of Mt. Halla is some higher. For pH and contents of total nitrogen and organic matter, Mt. Halla is higher than Mt. Jiri but for electrical conductivity , Mt. Jiri is relatively higher than Mt. Halla.

• Journal of Life Science
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• v.21 no.4
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• pp.529-533
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• 2011

Metallothioneins (MT) are a group of low-molecular weight, cysteine-rich, intracellular proteins that are encoded by a family of genes containing at least 10 functional isoforms in human. The expression and induction of these proteins is associated with protection against DNA damage, oxidative stress, and apoptosis. Many studies have shown increased expression of MT in various human tumors, whereas MT is down-regulated in certain tumors such as hepatocellular carcinoma and liver adenocarcinoma. Hence, the expression of MT is not universal to all human tumors but may depend on the differentiation status and proliferative index of tumors, along with other tissue factors and gene mutations. Using Northern blot analysis, we found that laminin induced expression of MT-1 in HSG and PC12 cells, which can be differentiated by laminin, but had no effect on MB-231, MDA-435, and PC-3 cells, which cannot be differentiated by laminin. In addition, we analyzed the expression level of the MT-1 gene in five prostate cancer cell lines possessing different metastatic potential. The expression of MT-1 in normal and less malignant cells (RWPE-1 and WPE1-NA22) was high and up-regulated by laminin, whereas the expression of MT-1 in WPE1-NB14, WPE1-NB11, and WPE1-NB26 cells (malignant) was extremely low and not elevated by laminin. These results suggest that the MT-1 gene is involved in laminin-mediated differentiation and affects the metastatic potential of tumor cells.

• Plant Resources
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• v.3 no.2
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• pp.138-147
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• 2000

This examination of vegetation is conducted from June to Oct. 1999 through on the spot survey, centering on the habitat of Abies koreana around Imgeolryeong, Jangteomok, and Jeseokbong in Mt Jiri and Yeongsil path and Seongpanak path in Mt. Halla. Species composition tables of the Abies koreana community by altitude and slope in Mt. Jiri and Mt. Halla are made based on the community composition tables examined in quadrat. The Abies koreana-Saso quelpaertensis community in a west slope of Mt. Halla is found that Abies koreana of 4-6m in height forms subtree layer and that of 8-9m in height the tree layer and it reflects a difference between community structure by slope and main composition species. While the tree layer of Abies koreana community is 12-14 in height around the area of 1,290-1,560m above the sea of Imgeolryong and path from Baekmudong to Jangteomok, it is 8-12m in height in the area of 1,680-1,780m above the sea of path from Jangteomok to Jeseokbong. It means that community structure depends on area and attitude. This study finds out that commonly appearing composition species similar to coverage and presence are Lepisorus thunberianus, Quercus mongolica, Solidago virga-aurea var. asiatica, Ligularia fischeri, Carex lanceolata, Clintonia udensis, Magnolia sieboldii, Betula ermani, Acer pseudo-sieboldianum. As soil environment of surveyed area, pH of surveyed spots is similar in Mt. Jiri and Mt. Halla, electrical conductivity is higher in Mt Jiri, and contents of organic matter is relatively higher in a west slope of Mt. Jiri and Mt. Halla and lower in their south and west slopes. Contents of organic matter and total nitrogen show that the area of 1,550m above the sea in a west slope of Mt. Halla is some higher. For pH and contents of total nitrogen and organic matter, Mt. Halla is higher than Mt. Jiri but for electrical conductivity, Mt. Jiri is relatively higher than Mt. Halla.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.16 no.5
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• pp.49-57
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• 2013

This study was carried out to analysis change of distribution and habitat location environment of Korean Fir which is typically vulnerable species by the climate change in Mt. Jiri and Halla. Korean Fir was decreased 18% during 27 year since year 1981, Mt. Halla was 34% during 15 years since year 1988. In the same periods, Temperature change was increased from 8.56 to $9.36^{\circ}C$, from 11.2 to $12.1^{\circ}C$. Distribution changes by the elevation showed higher change ratio 1,400~1,600m in Mt. Jiri and 1,200~1,900m in Mt. Halla. Changes of Korean Fir each slope aspects was high $180{\sim}360^{\circ}$ in Mt. Jiri, $45^{\circ}$ in Mt. Halla. In slope was $30^{\circ}$ in Mt. Jiri and $20^{\circ}$ in Mt. Halla. Changes by reliefs was 12 in Mt. Jiri, 0 or 15 in Mt. Halla, and Sites of Korean Fir was convex slopes both of two areas. Changes by soils was in the good drainage textures.