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http://dx.doi.org/10.5352/JLS.2011.21.4.529

Induction of Metallothionein Gene by Laminin in Normal and Malignant Human Prostate Epithelial Cells  

Ock, Mee-Sun (Department of Parasitology and Gnenetics, Kosin University College of Medicine)
Cha, Hee-Jae (Department of Parasitology and Gnenetics, Kosin University College of Medicine)
Publication Information
Journal of Life Science / v.21, no.4, 2011 , pp. 529-533 More about this Journal
Abstract
Metallothioneins (MT) are a group of low-molecular weight, cysteine-rich, intracellular proteins that are encoded by a family of genes containing at least 10 functional isoforms in human. The expression and induction of these proteins is associated with protection against DNA damage, oxidative stress, and apoptosis. Many studies have shown increased expression of MT in various human tumors, whereas MT is down-regulated in certain tumors such as hepatocellular carcinoma and liver adenocarcinoma. Hence, the expression of MT is not universal to all human tumors but may depend on the differentiation status and proliferative index of tumors, along with other tissue factors and gene mutations. Using Northern blot analysis, we found that laminin induced expression of MT-1 in HSG and PC12 cells, which can be differentiated by laminin, but had no effect on MB-231, MDA-435, and PC-3 cells, which cannot be differentiated by laminin. In addition, we analyzed the expression level of the MT-1 gene in five prostate cancer cell lines possessing different metastatic potential. The expression of MT-1 in normal and less malignant cells (RWPE-1 and WPE1-NA22) was high and up-regulated by laminin, whereas the expression of MT-1 in WPE1-NB14, WPE1-NB11, and WPE1-NB26 cells (malignant) was extremely low and not elevated by laminin. These results suggest that the MT-1 gene is involved in laminin-mediated differentiation and affects the metastatic potential of tumor cells.
Keywords
Metallothionein; laminin; prostate cancer; differentiation; malignant;
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1 Timpl, R., H. Rohde, P. G. Robey, S. I. Rennard, J. M. Foidart, and G. R. Martin. 1979. Laminin--a glycoprotein from basement membranes. J. Biol. Chem. 254, 9933-9937.
2 Weaver, V. M., A. H. Fischer, O. W. Peterson, and M. J. Bissell. 1996. The importance of the microenvironment in breast cancer progression: recapitulation of mammary tumorigenesis using a unique human mammary epithelial cell model and a three-dimensional culture assay. Biochem. Cell Biol. 74, 833-851.   DOI
3 Webber, M. M., D. Bello, H. K. Kleinman, and M. P. Hoffman. 1997. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18, 1225-1231.   DOI
4 Zeng, J., B. L. Vallee, and J. H. Kagi. 1991. Zinc transfer from transcription factor IIIA fingers to thionein clusters. Proc. Natl. Acad. Sci. USA 88, 9984-9988.   DOI
5 Graner, E., A. F. Mercadante, S. M. Zanata, V. R. Martins, D. G. Jay, and R. R. Brentani. 2000. Laminin-induced PC-12 cell differentiation is inhibited following laser inactivation of cellular prion protein. FEBS Lett. 482, 257-260.   DOI
6 Hecht, D., D. Jung, V. V. Prabhu, P. J. Munson, M. P. Hoffman, and H. K. Kleinman. 2002. Metallothionein promotes laminin-1-induced acinar differentiation in vitro and reduces tumor growth in vivo. Cancer Res. 62, 5370-5374.
7 Hoffman, M. P., M. C. Kibbey, J. J. Letterio, and H. K. Kleinman. 1996. Role of laminin-1 and TGF-beta 3 in acinar differentiation of a human submandibular gland cell line (HSG). J. Cell Sci. 109, 2013-2021.
8 Kleinman, H. K., M. L. McGarvey, L. A. Liotta, P. G. Robey, K. Tryggvason, and G. R. Martin. 1982. Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry 21, 6188-6193.   DOI
9 Hoffman, M. P., M. Nomizu, E. Roque, S. Lee, D. W. Jung, Y. Yamada, and H. K. Kleinman. 1998. Laminin-1 and laminin-2 G-domain synthetic peptides bind syndecan-1 and are involved in acinar formation of a human submandibular gland cell line. J. Biol. Chem. 273, 28633-28641.   DOI
10 Kleinman, H. K., M. L. McGarvey, J. R. Hassell, V. L. Star, F. B. Cannon, G. W. Laurie, and G. R. Martin. 1986. Basement membrane complexes with biological activity. Biochemistry 25, 312-318.   DOI
11 Lee, J. H. and D. R. Welch. 1997. Suppression of metastasis in human breast carcinoma MDA-MB-435 cells after transfection with the metastasis suppressor gene, KiSS-1. Cancer Res. 57, 2384-2387.
12 Lichtlen, P. and W. Schaffner. 2001. Putting its fingers on stressful situations: the heavy metal-regulatory transcription factor MTF-1. Bioessays 23, 1010-1017.   DOI
13 Malinda, K. M. and H. K. Kleinman. 1996. The laminins. Int. J. Biochem. Cell Biol. 28, 957-959.   DOI
14 Shirasuna, K., M. Sato, and T. Miyazaki. 1981. A neoplastic epithelial duct cell line established from an irradiated human salivary gland. Cancer 48, 745-752.   DOI
15 Alimirah, F., J. Chen, Z. Basrawala, H. Xin, and D. Choubey. 2006. DU-145 and PC-3 human prostate cancer cell lines express androgen receptor: implications for the androgen receptor functions and regulation. FEBS Lett. 580, 2294-2300.   DOI
16 Andrews, G. K., E. D. Adamson, and L. Gedamu. 1984. The ontogeny of expression of murine metallothionein: comparison with the alpha-fetoprotein gene. Dev. Biol. 103, 294-303.   DOI
17 Bello-DeOcampo, D., H. K. Kleinman, N. D. Deocampo, and M. M. Webber. 2001. Laminin-1 and alpha6beta1 integrin regulate acinar morphogenesis of normal and malignant human prostate epithelial cells. Prostate 46, 142-153.   DOI
18 Nartey, N., M. G. Cherian, and D. Banerjee. 1987. Immunohistochemical localization of metallothionein in human thyroid tumors. Am. J. Pathol. 129, 177-182.
19 Nishimura, H., N. Nishimura, and C. Tohyama. 1989. Immunohistochemical localization of metallothionein in developing rat tissues. J. Histochem. Cytochem. 37, 715-722.   DOI
20 Timpl, R. and J. C. Brown. 1994. The laminins. Matrix Biol. 14, 275-281.   DOI
21 Bello, D., M. M. Webber, H. K. Kleinman, D. D. Wartinger, and J. S. Rhim. 1997. Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18. Carcinogenesis 18, 1215-1223.   DOI
22 Cha, H. J., M. J. Jeong, and H. K. Kleinman. 2003. Role of thymosin beta4 in tumor metastasis and angiogenesis. J. Natl. Cancer Inst. 95, 1674-1680.   DOI   ScienceOn
23 Cherian, M. G., A. Jayasurya, and B. H. Bay. 2003. Metallothioneins in human tumors and potential roles in carcinogenesis. Mutat. Res. 533, 201-209.   DOI
24 Clarkson, J. P., M. E. Elmes, B. Jasani, and M. Webb. 1985. Histological demonstration of immunoreactive zinc metallothionein in liver and ileum of rat and man. Histochem. J. 17, 343-352.   DOI
25 Colognato, H. and P. D. Yurchenco. 2000. Form and function: the laminin family of heterotrimers. Dev. Dyn. 218, 213-234.   DOI
26 Danielson, K. G., S. Ohi, and P. C. Huang. 1982. Immunochemical detection of metallothionein in specific epithelial cells of rat organs. Proc. Natl. Acad. Sci. USA 79, 2301-2304.   DOI