• Asian Pacific Journal of Cancer Prevention
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• v.17 no.4
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• pp.1993-1998
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• 2016

The effects of carbon-ion irradiation on cancer cell telomere function have not been comprehensively studied. In our previous report cancer cells with telomere dysfunction were more sensitive to carbon-ion irradiation, but the underlying mechanisms remained unclear. Here we found that telomerase activity was suppressed by carbon-ion irradiation via hTERT down-regulation. Inhibition of telomere activity by MST-312 further increased cancer cell radiosensitivity to carbon-ion radiation. hTERT suppression caused by either carbon-ion irradiation or MST-312 impaired mitochondrial function, as indicated by decreased membrane potential, mtDNA copy number, mitochondrial mass, total ATP levels and elevated reactive oxygen species (ROS). PGC-$1{\alpha}$ expression was repressed after carbion-ion irradiation, and hTERT inhibition by MST-312 could further exacerbate this effect. Lowering the mitochondrial ROS level by MitoTEMPO could partially counteract the induction of cellular senescence induced by carbon-ion radiation and MST-312 incubation. Taken together, the current data suggest that telomere-mitochondrion links play a role in the induction of senescence in MCF-7 cells after carbon-ion irradiation.

• Nuclear Engineering and Technology
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• v.53 no.1
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• pp.208-215
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• 2021

Cosmic-ray muon scattering tomography (MST) technology is a new radiation imaging technology with unique advantages. As the performance of its image reconstruction algorithm has a crucial influence on the imaging quality, researches on this algorithm are of great significance to the development and application of this technology. In this paper, a fast inspection algorithm based on clustering analysis for the identification of the existence of nuclear materials is studied and optimized. Firstly, the principles of MST technology and a binned clustering algorithm were introduced, and then several simulation experiments were carried out using Geant4 toolkit to test the effects of exposure time, algorithm parameter, the size and structure of object on the performance of the algorithm. Based on these, we proposed two optimization methods for the clustering algorithm: the optimization of vertical distance coefficient and the displacement of sub-volumes. Finally, several sets of experiments were designed to validate the optimization effect, and the results showed that these two optimization methods could significantly enhance the distinguishing ability of the algorithm for different materials, help to obtain more details in practical applications, and was therefore of great importance to the development and application of the MST technology.

• Korean Journal of Acupuncture
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• v.21 no.1
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• pp.79-93
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• 2004

Objectives and methods : To study on the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Sinomenii acuti Lignum infusion solution(SAL-HAS), we injected Sinomenii acuti Lignum infusion solution into Joksamni$(ST_{36})$ of C57BL/6 mouse which is corresponding to human Joksamni(ST36). We observed its effect on the number of $CD25^+/CD4^+,\;CD8^+/CD3e^+,\;CD69^+/B220^+,\;NK1.1^+/CD3e^+$ cells in mouse PBMCs(peripheral blood mononuclear cells), the number of the pulmonary colony, and the effect on MST(mean survival time) and ILS(increase in MST over control) of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results and Conclusions : 1. The spleen cells proliferation of the sample groups treated with SAL-HAS extract has increased significantly compared with that of the control group. 2. The percentage of the $CD25^+/CD4^+,\;CD8^+/CD3e^+,\;CD69^+/B220^+,\;NK1.1^+/CD3e^+$ cells in C57BL/6 mouse PBMCs of the sample groups treated with SAL-HAS has increased compared with that of the control group. 3. The pulmonary colony number of the sample groups SAL-HAS has decreased significantly compared with that of the control group. 4. MST and ILS of the sample groups SAL-HAS have increased significantly compared with those of the control group.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.4
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• pp.107-116
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• 2013

This algorithm suggests a method in which a minimum spanning tree can be obtained fast by reducing the number of an algorithm execution. The suggested algorithm performs a select-and-delete process. In the select process, firstly, it performs Borůvka's first stage for all the vertices of a graph. Then it re-performs Borůvka's first stage for specific vertices and reduces the population of the edges. In the delete process, it deletes the maximum weight edge if any cycle occurs between the 3 edges of the edges with the reduced population. After, among the remaining edges, applying the valency concept, it gets rid of maximum weight edges. Finally, it eliminates the maximum weight edges if a cycle happens among the vertices with a big valency. The select-and-delete algorithm was applied to 9 various graphs and was evaluated its applicability. The suggested select process is believed to be the vest way to choose the edges, since it showed that it chose less number of big edges from 6 graphs, and only from 3 graphs, comparing to the number of edges that is to be performed when using MST algorithm. When applied the delete process to Kruskal algorithm, the number of performances by Kruskal was less in 6 graphs, but 1 more in each 3 graph. Also, when using the suggested delete process, 1 graph performed only the 1st stage, 5 graphs till 2nd stage, and the remaining till 3rd stage. Finally, the select-and-delete algorithm showed its least number of performances among the MST algorithms.

• The Korean Journal of Applied Statistics
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• v.31 no.4
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• pp.517-528
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• 2018

We provide R scripts to detect outliers in multivariate data and visualization. Detecting outliers is provided using three approaches 1) Robust Mahalanobis distance, 2) High Dimensional data, 3) density-based approach methods. We use the following techniques to visualize detected potential outliers 1) multidimensional scaling (MDS) and minimal spanning tree (MST) with k-means clustering, 2) MDS with fviz cluster, 3) principal component analysis (PCA) with fviz cluster. For real data sets, we use MLB pitching data including Ryu, Hyun-jin in 2013 and 2014. The developed R scripts can be downloaded at "http://www.knou.ac.kr/~sskim/ddpoutlier.html" (R scripts and also R package can be downloaded here).

• Toxicological Research
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• v.34 no.2
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• pp.133-141
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• 2018

Anti-cancer drugs such as cisplatin and doxorubicin are effectively used more than radiotherapy. Cisplatin is a chemotherapeutic drug, used for treatment of various forms of cancer. However, it has side effects such as ototoxicity and nephrotoxicity. Cisplatin-induced nephrotoxicity increases tubular damage and renal dysfunction. Consequently, we investigated the beneficial effect of cynaroside on cisplatin-induced kidney injury using HK-2 cell (human proximal tubule cell line) and an animal model. Results indicated that $10{\mu}M$ cynaroside diminished cisplatin-induced apoptosis, mitochondrial dysfunction and caspase-3 activation, cisplatin-induced upregulation of caspase-3/MST-1 pathway decreased by treatment of cynaroside in HK-2 cells. To confirm the effect of cynaroside on cisplatin-induced kidney injury in vivo, we used cisplatin exposure animal model (20 mg/kg, balb/c mice, i.p., once a day for 3 days). Renal dysfunction, tubular damage and neutrophilia induced by cisplatin injection were decreased by cynaroside (10 mg/kg, i.p., once a day for 3 days). Results indicated that cynaroside decreased cisplatin-induced kidney injury in vitro and in vivo, and it could be used for improving cisplatin-induced side effects. However, further experiments are required regarding toxicity by high dose cynaroside and caspase-3/MST-1-linked signal transduction in the animal model.

• Journal of applied mathematics & informatics
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• v.7 no.2
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• pp.629-640
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• 2000

this paper present an efficient line-drawing algorithm that reduces the amount of space required, Because of its efficiency , this line-drawing algorithm is faster than the Bresenham algorithm or the recursive bisection method. this efficiency was achieved through a new data structure; namely , the modified segment tree (MST). Using the modified segment tree and the distribution rule suggested in this paper, we dra lines without generating the recursive calls used in [3] and without creating the binary operation used in [4]. we also show that line accuracy improves in proportion to the display resolution . In practice, we can significantly improve the algorithm's performance with respect to time and space, This improvement offer an increase in speed, specially with lines at or near horizontal, diagonal. or vertical ; that is, this algorithm requires the time complexity of (n) and the space complexity O(2k+1), where n is the number of pixels and k is a level of the modified segment tree.

• Journal of Korean Institute of Industrial Engineers
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• v.31 no.1
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• pp.10-19
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• 2005

The minimum spanning tree (MST) problem is one of the traditional optimization problems. Unlike the MST, the degree constrained minimum spanning tree (DCMST) of a graph cannot, in general, be found using a polynomial time algorithm. So, finding the DCMST of a graph is a well-known NP-hard problem of importance in communications network design, road network design and other network-related problems. So, it seems to be natural to use evolutionary algorithms for solving DCMST. Especially, when applying an evolutionary algorithm to spanning tree problems, a representation and search operators should be considered simultaneously. This paper introduces a new tree representation scheme and a genetic operator for solving combinatorial tree problem using evolutionary algorithms. We performed empirical comparisons with other tree representations on several test instances and could confirm that the proposed method is superior to other tree representations. Even it is superior to edge set representation which is known as the best algorithm.

• Korean Management Science Review
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• v.23 no.1
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• pp.75-85
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• 2006

The rectilinear Steiner tree problem (RSTP) is to find a minimum-length rectilinear interconnection of a set of terminals in the plane. It is well known that the solution to this problem will be the minimal spanning tree(MST) on some set Steiner points. The RSTP is known to be NP-complete. The RSTP has received a lot of attention in the literature and heuristic and optimal algorithms have been proposed. A key performance measure of the algorithm for the RSTP is the reduction rate that is achieved by the difference between the objective value of the RSTP and that of the MST without Steiner points. A hybrid evolutionary strategy on RSTP based upon nodes set is presented. The computational results show that the hybrid evolutionary strategy is better than the previously proposed other heuristic. The average reduction rate of solutions from the evolutionary strategy is about 11.14%, which is almost similar to that of optimal solutions.

• Natural Product Sciences
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• v.10 no.3
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• pp.134-139
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• 2004

In this study, an immunoactivity of panaxtriol ginsenosides Re and Rg1 against infection due to Candida albicans was investigated. The ginsenosides were extracted from Red Ginseng with 85% ethanol and heat-treatment and were analyzed by HPLC on water-acetonitrile as a mobile phase. The HPLC analysis revealed that the extract contained ginsenosides Re and Rg1, which were eluted as a combined peak. By agar diffusion susceptibility, the mixture of Re and Rg1 had no growth-inhibitory activity on C. albicans yeast cells. However, in animal tests BALB/c mice given the mixture of Re and Rg1 intraperitoneally (Lp.) before intravenous (Lv.) infection with live C. albicans yeast cells had longer mean survival times (MST) than MST of control mice groups that received only buffer solution instead of Re and Rg1. In experiments 60% of the ginsenosides-treated mice survived the entire duration of the 50-day observation. The Re and Rg1 mixture induced production of nitric oxide when interacted with RAW 264.7 macrophage cell line. In addition, the mixture caused morphological change of the macrophages. These data indicate that immunostimulation by the Re and Rg1 may be responsible for the protection of mice against disseminated candidiasis.