• Proceedings of the Korea Society of Environmental Biology Conference
• /
• 2002.05a
• /
• pp.13-13
• /
• 2002

• IMMUNE NETWORK
• /
• v.2 no.4
• /
• pp.208-216
• /
• 2002

Background: Reactive oxygen and nitrogen are produced by rheumatoid arthritis (RA) synovial tissue and can induce mutations in key genes. Normally, this process is prevented by a DNA mismatch repair (MMR) system that maintains sequence fidelity. Key members of the MMR system include MutS${\alpha}$ (comprised of hMSH2 and hMSH6), which can sense and repair single base mismatches and 8-oxoguanine, and MutS${\beta}$ (comprised of hMSH2 and hMSH3), which repairs longer insertion/deletion loops. Methods: To provide further evidence of DNA damage, we analyzed synovial tissues for microsatellite instability (MSI). MSI was examined by PCR on genomic DNA of paired synovial tissue and peripheral blood cells (PBC) of RA patients using specific primer sequences for 5 key microsatellites. Results: Surprisingly, abundant MSI was observed in RA synovium compared with osteoarthritis (OA) tissue. Western blot analysis of the same tissues for the expression of MMR proteins demonstrated decreased hMSH6 and increased hMSH3 in RA synovium. To evaluate potential mechanisms of MMR regulation in arthritis, fibroblast-like synoviocytes (FLS) were isolated from synovial tissues and incubated with the nitric oxide donor S-nitroso-N-acetylpenicillamine (SNAP). Western blot analysis demonstrated constitutive expression of hMSH2, 3 and 6 in RA and OA FLS. When FLS were cultured with SNAP, the RA synovial pattern of MMR expression was reproduced (high hMSH3, low hMSH6). Conclusion: Therefore, oxidative stress can relax the DNA MMR system in RA by suppressing hMSH6. Decreased hMSH6 can subsequently interfere with repair of single base mutations, which is the type observed in RA. We propose that oxidative stress not only creates DNA adducts that are potentially mutagenic, but also suppresses the mechanisms that limit the DNA damage.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.17 no.7
• /
• pp.1515-1521
• /
• 2013

AIS is a core service in the maritime safety domain. AIS Message type #6 and #8(for binary date) can be used for carrying any kind of MSI(Maritime Safety Information). The MSC(Maritime Safety Committee) approved SN/Circ. 289 on Guidance on the use of AIS Application-Specific Messages in 2010. The MSI that carried by AIS-ASM can support to reduce the maritime accidents. Since a new AIS-ASM service places an additional load on the VDL(VHF Data Link), there should be a deployment process for new AIS-ASM services. In this paper we analyze the AIS-ASM services and survey the extended functions and systems for each AIS-ASM service. We also present the survey of the statistical analysis of local AIS messages. and suggest preliminary research topics for the deployment of new AIS-ASM services. VDL monitoring system, system for detection and removal of malfunctioning AIS equipments, and value analysis process of the MSI services are required.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2012.10a
• /
• pp.123-124
• /
• 2012

Over the past decades, information and communication service providing safe and efficient navigation have been tried in maritime area. However, there were provided different ways such as different protocol or different policy. It made hard to serve efficiently. The introduction of MSI as a part of e-navigation development can be a kind of concepts to solve this problem. This paper tries to suggest a conceptual design of onboard application middleware to be able to communicate the shore-side maritime safety information system, take into account the reuse of open-source software.

• Biomedical Science Letters
• /
• v.28 no.4
• /
• pp.298-306
• /
• 2022

Pathological tissue fixation using formalin has been widely used for histological samples in many hospitals and institutions. In general, formalin fixatives were either manufactured in laboratories or purchased commercially because of the risks and environmental concerns of handling organic compounds. In this study, the efficacy of three kinds of commercially purchased and one laboratory-made formalin fixative was compared in the PCR-based molecular diagnosis using the extracted DNA from formalin-fixed paraffin-embedded (FFPE) tissues. The quality of extracted DNA from FFPE tonsil tissues with four kinds of formalin solutions was evaluated, and PCR for beta-globin gene and microsatellite instabilities (MSI) tests for pentaplex panel markers were performed using the extracted DNA. There was no difference in PCR and MSI tests as molecular diagnoses regardless of the types of formalin used in this study. However, the total amount and average length of double-stranded DNA extracted from FFPE tonsil tissue showed significant differences according to the type of formalin fixative. Optimized formalin fixatives and methods for DNA extraction might be sophisticated to extract good quality DNA from the small size of specific tissue samples. Further studies are needed to select the most effective formalin fixative for histology and molecular pathology using human FFPE tissues.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.10
• /
• pp.151-157
• /
• 2018

Among the various sensors for measuring traffic, PVDF (polyvinylidene fluoride) piezoelectric sensors are used to classify vehicles because they can detect the axle of the vehicle. Piezoelectric sensors are embedded in road pavements and are always exposed to traffic loads and environmental loads. Therefore, the life expectancy is very short, less than 6 years. Traffic control is essential for reinstallation and data collection is interrupted during the failure period. The lifespan will increase if the sensor installation depth is increased. In this study, the sensor signal output was analyzed with a variable depth of sensor installation to verify the possibility of deeper installation. Furthermore, various parameters, such as the weight and speed, were analyzed. The wheel load is applied using APT. As a result, the MSI BL sensor output signal is higher than 100mV when installed at 3cm, which is reliable. If the location of the sensor is deeper in the pavement, the expected lifetime of the sensor is also increased. On the other hand, the MSI cable was found to be less than 100mV at the shallowest depth of 1cm, making it impossible for field applications.

• Composites Research
• /
• v.36 no.3
• /
• pp.205-210
• /
• 2023

In this study, we developed a transfer learning framework based on homogenization data for efficient prediction of the effective mechanical properties and thermal conductivity of cellular foam structures. Mean-field homogenization (MFH) based on the Eshelby's tensor allows for efficient prediction of properties in porous structures including ellipsoidal inclusions, but accurately predicting the properties of cellular foam structures is challenging. On the other hand, finite element homogenization (FEH) is more accurate but comes with relatively high computational cost. In this paper, we propose a data-driven transfer learning framework that combines the advantages of mean-field homogenization and finite element homogenization. Specifically, we generate a large amount of mean-field homogenization data to build a pre-trained model, and then fine-tune it using a relatively small amount of finite element homogenization data. Numerical examples were conducted to validate the proposed framework and verify the accuracy of the analysis. The results of this study are expected to be applicable to the analysis of materials with various foam structures.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.43 no.6
• /
• pp.883-896
• /
• 2023

As human activities in Korea are spread throughout the mountains, forest fires often affect residential areas, infrastructure, and other facilities. Hence, it is necessary to detect fire-damaged areas quickly to enable support and recovery. Remote sensing is the most efficient tool for this purpose. Fire damage detection experiments were conducted on the east coast of Korea. Because this area comprises a mixture of forest and artificial land cover, data with low resolution are not suitable. We used Sentinel-2 multispectral instrument (MSI) data, which provide adequate temporal and spatial resolution, and the k-nearest neighbor (kNN) algorithm in this study. Six bands of Sentinel-2 MSI and two indices of normalized difference vegetation index (NDVI) and normalized burn ratio (NBR) were used as features for kNN classification. The kNN classifier was trained using 2,000 randomly selected samples in the fire-damaged and undamaged areas. Outliers were removed and a forest type map was used to improve classification performance. Numerous experiments for various neighbors for kNN and feature combinations have been conducted using bi-temporal and uni-temporal approaches. The bi-temporal classification performed better than the uni-temporal classification. However, the uni-temporal classification was able to detect severely damaged areas.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2002.10a
• /
• pp.92-92
• /
• 2002

• Proceedings of KOSOMES biannual meeting
• /
• 2017.04a
• /
• pp.96-96
• /
• 2017