• Molecules and Cells
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• 제42권4호
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• pp.356-362
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• 2019

The binding of MS2 bacteriophage coat protein (MCP) to MS2 binding site (MBS) RNA stem-loop sequences has been widely used to label mRNA for live-cell imaging at single-molecule resolution. However, concerns have been raised recently from studies with budding yeast showing aberrant mRNA metabolism following the MS2-GFP labeling. To investigate the degradation pattern of MS2-GFP-labeled mRNA in mammalian cells and tissues, we used Northern blot analysis of ${\beta}$-actin mRNA extracted from the Actb-MBS knock-in and $MBS{\times}MCP$ hybrid mouse models. In the immortalized mouse embryonic cell lines and various organ tissues derived from the mouse models, we found no noticeable accumulation of decay products of ${\beta}$-actin mRNA compared with the wild-type mice. Our results suggest that accumulation of MBS RNA decay fragments does not always happen depending on the mRNA species and the model organisms used.

• Journal of Plant Biotechnology
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• 제7권2호
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• pp.97-103
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• 2005

This study was carried out to obtain basic information for gene manipulation in potent edible tobacco (Nicotiana tabacum cv. TI 516). N. tabacum cv. TI 516 is a plant for a possible candidate to use as an edible vaccine, since it contains a low level of nicotine. The effective plant regeneration system through leaf disc culture was achieved using a MS basal medium supplemented with 0.1 mg $1^{-1}$ NAA and 0.5 mg $1^{-1}$ BA. In order to transform the N. tabacum cv. TI 516 with the green fluorescent protein (GFP) gene, Agrobacterium tumefaciens LBA 4404 containing the GFP gene was used. Genomic PCR confirmed the integration of the GFP gene into nuclear genome of transgenic plants. Expression of the GFP gene was identified in callus, apical meristem and root tissue of transgenic N. tabacum cv. TI 516 plants using fluorescence microscopy. Western blot analysis revealed the expression of GFP protein in the transgenic edible tobacco plants. The amount of GFP protein detected in the transgenic tobacco plants was approximately 0.16% of the total soluble plant protein (TSP), which was determined by ELISA.

• Journal of Plant Biotechnology
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• 제33권1호
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• pp.33-37
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• 2006

박과 (Cucurbitaceae) 작물에서 박 (Lagenaria siceraria Standl.)은 식용뿐만 아니라 주로 저온 신장성의 확보와 토양전염성 병해를 회피하기 위한 수단으로서 수박의 대목으로 많이 사용된다. 유전공학 방법을 이용한 토양병 내성이 증대된 신품종 개발이 필요한 요즘 박 형질전환체 개발의 효과적인 시스템 확립을 위하여 식물형질전환 연구에 매우 효율적으로 이용되는 마커인 GFP 유전자를 도입하였다. 실험재료로 이용된 박 계통은 9005, 9006 및 G5였으며, 신초 유기를 위한 선발 배지는 3.0 mg/L_ BAP + 100 mg/L kanamycin +500 mg/L cefotaxime + 0.5 mg/L $AgNO_3$, pH 5.8이었다 박 선발배지에 치상된 자엽 절편체는 3주 정도 지나면 절단면 즉 치상 부위에서 작은 돌기들이 발생하였고, 갈수록 절편체는 갈변하여 고사하였다. 선발된 개체에서는 신초가 발생하였으며 선발 후 7주가 경과하면 pot로 이식하여 발근된 완전한 식물체를 얻을 수 있었다. 본 실험결과 박 형질전환 시 genotype에 따른 신초 발생률에 상당한 차이를 보였는데 계통 9005의 경우 신초 발생율이 0.0%이였으며 계통 G5의 경우 4.1%이였다. 형질전환으로 발생된 신초는 GFP가 발현되지 않는 것과 GFP가 발현되는 것 두 가지 양상을 볼 수 있었다. PCR 및 Southern blot 분석을 한 결과, 9006과 G5 두 genotype에서 형질전환 신초에서 GFT 유전자를 가지고 있는 것으로 확인되었으며 GFP 유전자가 도입된 박 형질전환체를 얻을 수 있었다. 따라서 GFP 유전자는 박 형질전환시 유식물체에서 형질전환체를 쉽게 선발할 수 있는 유용한 마커로 이용 될 수 있다.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회/대한산업공학회 2003년도 춘계공동학술대회
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• pp.800-807
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• 2003

Decomposition of tasks in the ordinary manufacturing systems is usually based on the predefined goal of the system. To achieve the high-level-goals (e.g., factory goal or company goal), several sub-goals should be achieved in advance. However, goals can change along with the current status of the system and the external environmental situations. Thus, a manufacturing system should support the goal-formations which can be bearable these changes for efficient and effective operations. Therefore, it IS necessary to develop a systematic methodology for the goal-formations in a manufacturing system. Especially, the formation and/or change of goals in real-time should be possible for distributed and dynamic systems including the fractal manufacturing system (FrMS). In this paper, a threefold methodology is proposed for the goal-formation process (GFP) in the FrMS; 1) a goal­generating process (GGP) to make and propagate fuzzy goals, 2) a goal-harmonizing process (GHP) to eliminate or reduce conflicts and interferences of goals by using a mobile agent- based negotiation scheme, and 3) a goal-balancing process (GBP) to make a compromise between goals by using quantifiable indicators of the manufacturing system.