Expression of the Green Fluorescent Protein (GFP) in Tobacco Containing Low Nicotine for the Development of Edible Vaccine

  • Kim Young-Sook (Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Kim Mi-Young (Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University) ;
  • Kang Tae-Jin (Institute of Basic Science, Chonbuk National University) ;
  • Kwon Tae-Ho (Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Jang Yong-Suk (Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Yang Moon-Sik (Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University)
  • Published : 2005.06.01

Abstract

This study was carried out to obtain basic information for gene manipulation in potent edible tobacco (Nicotiana tabacum cv. TI 516). N. tabacum cv. TI 516 is a plant for a possible candidate to use as an edible vaccine, since it contains a low level of nicotine. The effective plant regeneration system through leaf disc culture was achieved using a MS basal medium supplemented with 0.1 mg $1^{-1}$ NAA and 0.5 mg $1^{-1}$ BA. In order to transform the N. tabacum cv. TI 516 with the green fluorescent protein (GFP) gene, Agrobacterium tumefaciens LBA 4404 containing the GFP gene was used. Genomic PCR confirmed the integration of the GFP gene into nuclear genome of transgenic plants. Expression of the GFP gene was identified in callus, apical meristem and root tissue of transgenic N. tabacum cv. TI 516 plants using fluorescence microscopy. Western blot analysis revealed the expression of GFP protein in the transgenic edible tobacco plants. The amount of GFP protein detected in the transgenic tobacco plants was approximately 0.16% of the total soluble plant protein (TSP), which was determined by ELISA.

Keywords

References

  1. Chalfie M, Tu Y, Euskirchen G, Ward WIN, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805 https://doi.org/10.1126/science.8303295
  2. Colby SM, Meredith CP (1990) Kanamycin sensivity of cultured tissues of vitis. Plant Cell Rep 9: 237-240
  3. Daniell H, Lee SB, Panchal T, Wiebe PO (2001) Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts. J Mol Biol 311: 1001-9 https://doi.org/10.1006/jmbi.2001.4921
  4. Elliott AR, Campbell JA, Dugdale B, Brettell RIS, Grof CPL (1999) Green- fluorescent protein facilitates rapid in vivo detection of genetically transformed plant cells. Plant Cell Rep 18: 707-714 https://doi.org/10.1007/s002990050647
  5. Halfhill MD, Richards HA, Mabon SA (2001) Expression of GFP and Bt transgenes in Brassica napus and hybridization with Brassica rapa. Theor Appl Genet 103: 659-667 https://doi.org/10.1007/s001220100613
  6. Haseloff J, Amos B (1995) GFP in plants. Trends in Genetics 11: 328-329 https://doi.org/10.1016/0168-9525(95)90186-8
  7. Hoekema A, Hirsch PR, Hooukaas PJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir-and T-region of the Agrobacterium tumefaciens Tiplasmid. Nature 303: 79-180
  8. Hu W, Cheng CL (1995) Expression of Aequorea green fluorescent protein in plant cells. FEBS Letters 369: 331-334 https://doi.org/10.1016/0014-5793(95)00776-6
  9. Huang Z, Dry I, Webster D, Strugnell R, Wesselingh S (2001) Plant-derived measles virus hemaglutinin protein induces neutralizing antibodies in mice. Vaccine 19: 2163-71 https://doi.org/10.1016/S0264-410X(00)00390-X
  10. Jani D, Singh NK, Bhattacharya S, Meena LS, Singh Y, Upadhyay SN, Sharma AK, Tyagi AK (2004) Studies on the immunogenic potential of plant-expressed cholera toxin B subunit. Plant Cell Rep 22: 471-477 https://doi.org/10.1007/s00299-003-0711-9
  11. Kang TJ, Loc NH, Jang MO, Jang YS, Kim YS, Seo JE, Yang MS (2003) Expression of the B subunit of E. coli heat-labile enterotoxin in the chloroplasts of plants and its characterization. Transgenic Res 12 : 683-691 https://doi.org/10.1023/B:TRAG.0000005114.23991.bc
  12. Kim CK, Chung JD, Park SH, Burrell AM, Kamo KK, Byrne DH (2004) Agrobacterium tumefaciens-mediated transformation of Rosa hybrida using the green fluorescent protein (GFP) gene. Plant Cell Tiss Org Cult 78: 107-111 https://doi.org/10.1023/B:TICU.0000022529.16697.90
  13. Kwon TH, Shin YM, Kim YS, Jang YS, Yang MS (2003) Secretory production of hGM-CSF with a high specific biological activity by transgenic plant cell suspension culture. Biotecnol Bioprocess Eng 8: 135-141 https://doi.org/10.1007/BF02940269
  14. Murashige T, Skoog F (1962) A revesed medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Niedz RP, Sussman MR, Satterlee JS (1995) Green fluorescent protein: an in vivo reporter of plant gene expression. Plant Cell Rep 14: 403-406
  16. Ponappa T, Brzozowski AE, Finer JJ (1999) Transient expression and stable transformation of soybean using the jellyfish green fluorescent protein. Plant Cell Rep 19: 6-12 https://doi.org/10.1007/s002990050702
  17. Sheen J, Hwang SB, Niwa Y, Kobayashi H, Galbraith DW (1995) Green fluorescent protein as a new vital marker in plant cells. Plant J 8: 777-778 https://doi.org/10.1046/j.1365-313X.1995.08050777.x
  18. Sidorov VA, Kasten D, Pang SZ, Peter TJH, Jeffrey MS, Narender SN (1999) Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker. Plant J 19: 209-216 https://doi.org/10.1046/j.1365-313X.1999.00508.x
  19. Stewart CN (2001) The utility of green fluorescent protein in transgenic plants. Plant Cell Rep 20: 376-382 https://doi.org/10.1007/s002990100346
  20. Tregoning JS, Nixon P, Kuroda H, Svab Z, Clare S, Bowe F, Fairweather N, Ytterberg J, van Wijk KJ, Dougan G, Maliga P (2003) Expression of tetanus toxin fragment C in tobacco chloroplasts. Nucleic Acids Res. 31: 1174-1179 https://doi.org/10.1093/nar/gkg221
  21. Varsani A, Williamson AL, Rose RC, Jaffer M, Rybicki EP (2003) Expression of human papillomavirus type 16 major capsid protein in transgenic Nicotiana tabacum cv. Xanthi. Arch Virol 148: 1771-1786 https://doi.org/10.1007/s00705-003-0119-4
  22. Yang TT, Ching L, Kain SR (1996) Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res 24: 4592-4593 https://doi.org/10.1093/nar/24.22.4592
  23. Zhang GG, Rodrigues L, Rovinski B, White KA (2002) Production of HIV-1 p24 protein in transgenic tobacco plants. Mol Biotechnol 20: 131-6 https://doi.org/10.1385/MB:20:2:131