• Journal of The Korean Society of Inherited Metabolic disease
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• v.3 no.1
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• pp.86-93
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• 2003

Background : The SCL began screening of newborns and high risk group blood spots with tandem mass spectrometry (MS/MS) in April 2001. Our goal was to determine approximate prevalence of metabolic disorders, optimization of decision criteria for estimation of preventive effect with early diagnosis. This report describes the ongoing effort to identify more than 30 metabolic disorders by MS/MS in South Korea. Methods : Blood spot was collected from day 2 to 30 (mostly from day 2 to 10) after birth for newborn. Blood spot of high risk group was from the pediatric patients in NICU, developmental delay, mental retardation, strong family history of metabolic disorders. One punch (3.2 mm ID) of dried blood spots was extracted with $150{\mu}L$ of methanol containing isotopically labelled amino acids (AA) and acylcarnitines (AC) internal standards. Butanolic HCl was added and incubated at $65^{\circ}C$ for 15 min. The butylated extract was introduced into the inlet of MS/MS. Neutral loss of m/z 102 and parent ion mode of m/z 85 were set for the analyses of AA and AC, respectively. Diagnosis was confirmed by repeating acylcarnitine profile, urine organic acid and plasma amino acid analysis, direct enzyme assay, or molecular testing. Results : Approximately 31,000 neonates and children were screened and the estimated prevalence (newborn/high risk group), sensitivity, specificity and recall rate amounted to 1:2384/1:2066, 96.55%, 99.98%, and 0.73%, respectively. Confirmed 28 (0.09%) multiple metabolic disorders (newborn/high risk) were as follows; 13 amino acid disorders [classical PKU (3/4), BH4 deficient-hyperphenylalaninemia (0/1), Citrullinemia (1/0), Homocystinuria (0/2), Hypermethioninemia (0/1), Tyrosinemia (1/0)], 8 organic acidurias [Propionic aciduria (2/1), Methylmalonic aciduria (0/1), Isovaleric aciduria (1/1), 3-methylcrotonylglycineuria (1/0), Glutaric aciduria type1 (1/0)], 7 fatty acid oxidation disorders [LCHAD def. (2/2), Mitochondrial TFP def. (0/1), VLCAD def. (1/0), LC3KT def. (0/1). Conclnsion : The relatively normal development of 10 patients with metabolic disorders among newborns (except for the expired) demonstrates the usefulness of newborn screening by MS/MS for early diagnosis and medical intervention. However, close coordination between the MS/MS screening laboratory and the metabolic clinic/biochmical geneticists is needed to determine proper decision of screening parameters, confirmation diagnosis, follow-up scheme and additional tests.

• Journal of Food Hygiene and Safety
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• v.32 no.2
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• pp.141-146
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• 2017

A rapid and precise method using LC-MS/MS was developed for carbendazim analysis in agricultural products. This compound was extracted with acetonitrile from agricultural products and cleaned up by solid-phase extraction procedure. The limit of detection and quantification were 0.001 mg/kg and 0.004 mg/kg, respectively. The mean recoveries and precision from 4 agricultural products, soybean sprout and mungbean sprout were in the range of 83.3-86.4% and 0.2-3.0% spiked at 1.0 mg/kg and those were in the range of 77.3-90.1% and 1.3-3.8% spiked at 0.02 mg/kg. The present method is faster and more precise compared with the multi-residue method of Korean Food Code. Therefore, we conclude that this method is suitable for carbendazim determination in a wide range of agricultural products.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.7 no.4
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• pp.678-683
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• 2003

This study presents a result of performance with the design of network topology for voice and data integration under computer network. This network is consisted of FastEthernet, other LANs and ATM WAN(wide area network), and performance evaluation of delay in a PBX+IP network, delay in a VoIP network and delay in a IP+ATM network will be shown. We use parameters including network bandwidth, number of packet, routing protocol(IGRP, OSPF). We simulate integrated of voice and data used PBX. we will study further about the case of integrated of voice and data environments using PBX. and, evaluate IP+ATM WAN average measured network delay and average delay of VoIP network.

• Journal of Sericultural and Entomological Science
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• v.35 no.1
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• pp.43-47
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• 1993

Using the new scouring soap made from rapeseed oil, various scouring test was done souring effects of new scouring soap were estimated. In the effects of scouring soap on the degumming ratio, OS (the soap made from oleic acid) was the most excellent and the next was RS (the soap made from rapeseed oil). Both OS and RS were more excellent than MS (the soap on the market). In a buffer power of soap solution, OS and RS were good, but MS was poor. The uneven dyeing was not observed in the silk fabric degummed by OS and RS, but observed in silk fabric degummed by MS. The value of tenacity and elongation in the silk degummed by OS and RS was higher that degummed by MS. In the crease recovery, softness and whiteness, the order of value was OS, RS, MS.

• Korean Journal of Plant Resources
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• v.16 no.1
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• pp.34-39
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• 2003

Corm apical meristem cultures of thirteen glasshouse freesia cultivars were tested to investigate the possibility of micropropagation using MS basal medium supplemented with 2,4-D, NAA(0.1, 0.5, 1.0mg/L, respectively) and BA (0.5∼2.0mg/L). The majority of the tested cultivars could be induced callus and shoot buds in all culture condition. The combinations of NAA and BA appeared superior to that of 2,4-D and BA depending on cultivars for callus induction and shoot formation. Among the cultivars, 'Golden Yellow' showed the highest regeneration capacity on MS media with 0.5mg/L NAA and 1.0 mg/L BA. The highest percentage of regeneration and the greatest number of shoot from calli were obtained through successive subculture on MS medium supplimented with 0.5mg/L BA. In that condition, more than 60 ％ shoot regeneration and average of 25.1 shoots per explant was achieved. Transformed shoots on half-strength MS medium without plant growth regulators rooted easily.

• Journal of Biomedical and Translational Research
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• v.19 no.4
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• pp.130-139
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• 2018

This study aimed to analyze a high-performance liquid chromatography (HPLC) separation using a pentafluorophenyl column of parent drug hydroxychloroquine (HCQ) and its active metabolite, desethylhydroxchloroquine (DHCQ) applying to determine bioequivalence of two different formulations administered to patients. A rapid, simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for bioanalysis of HCQ and its metabolite DHCQ in human whole blood using deuterium derivative $hydroxychloroquine-D_4$ as an internal standard (IS). A triple-quadrupole mass spectrometer was operated using electrospray ionization in multiple reaction monitoring (MRM) mode. Sample preparation involves a two-step precipitation of protein techniques. The removed protein blood samples were chromatographed on a pentafluorophenyl (PFP) column ($50mm{\times}4.6mm$, $2.6{\mu}m$) with a mobile phase (ammonium formate solution containing dilute formic acid) in an isocratic mode at a flow rate of 0.45 mL/min. The standard curves were found to be linear in the range of 2 - 500 ng/mL for HCQ; 2 - 2,000 ng/mL for DHCQ in spite of lacking a highly sensitive MS spectrometry system. Results of intra- and inter-day precision and accuracy were within acceptable limits. A run time of 2.2 min for HCQ and 2.03 min for DHCQ in blood sample facilitated the analysis of more than 300 human whole blood samples per day. Taken together, we concluded that the assay developed herein represents a highly qualified technology for the quantification of HCQ in human whole blood for a parallel design bioequivalence study in a healthy male.

• Journal of the Korean Society of Clothing and Textiles
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• v.31 no.6 s.165
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• pp.859-868
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• 2007

Degradation products of the dye extracted from turmeric and the turmeric dyed textiles were examined by using GC-MS after 100 oven (OV) and $H_2O_2/UV/O_2$(PER) treatments for up to 28 days. Throughout the OV degradation times, 2-propenoic acid, 3-(2-hydroxyphenyl)- was found consistently, while isovanillin, and vanillic acid were newly detected. In 28 day PER degradation sample, feruloylmethane, 2-propenoic acid, 3-(2-hydroxyphenyl)-, benzoic acid, and vanillic acid were detected as well as isovanillin. Feruloylrnethane, and 2-propenoic acid, 3-(2-hydroxyphenyl)- were detected from the degraded fabric samples. With the absence of curcuminoids in the GC-MS result, the decreasing pattern of 2-propenoic acid, 3-(2-hydrokyphenyl)- reflect the degradation of curcuminoids in turmeric extraction with the progression of OV degradation times. It is suggested that isovanillin, feruloylmethane, 2-propenoic acid,3-(2-hydroxyphenyl)-, and vanillic acid are the probable fingerprint products for determining the turmeric dye from the badly faded archaeological textiles.

• Journal of KIISE:Computer Systems and Theory
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• v.26 no.4
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• pp.389-405
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• 1999

본 논문에서는 상호 연결망의 노드를 행렬로 표현하고 행렬연산을 이용하여 에지를 정의한 새로운 상호 연결망으로 행렬-스타 그래프를 제안한다. 행렬-스타 그래프는 널리 알려진 스타 그래프를 일반화한 그래프이다. 먼저, 행렬-스타 그래프의 노드를 2 $\times$ n 행렬로 표현한 행렬-스타 그래프 MS2,n 에 대하여 주요 망 척도인 분지수, 연결도, 확장성, 대칭성, 리우팅 ,지름 방송등을 분석한다. 다음으로, 행렬-스타 그래프 MS2,n의 노드를 2차원과 3차원으로 일반화한 행렬-스타 그래크 MSk,n과 MS k,n,p를 정의하고 행렬-스타그래프 MSk,n,p 의 라우팅 알고리즘과 지름을 분석한다. 상호연결망의 중요 망 척도중 하나는 망 비용이고 상호연결망의 망 비용은 그 연결망의 분지수와 지름의 곱으로 정의된다. star 그래프는 다른 상호 연결망보다 작은 망 비용을 갖는다. 최근에 제안된 Macro-Star 그래픈 star 그래프에 비해 상대적으로 망 비용이 작은 값을 갖는 연결망이다. (n2)!개의 노드를 갖는 행렬-스타 그래프 MSk,k,k(k={{{{ `^{ 3} SQRT { n$^2$} }}}} )와 ((n-1)2 + 1)!개의 노드를 갖는 Macro-Star 그래프 MS(n-1, n-1)의 망 비용은 행렬-스타그래프 MSk,k,k(k={{{{ `^{ 3} SQRT { n$^2$} }}}})는 O(n2,7)이고, Macro-Star 그래프 MS(n-1 , n-1)은 O(n3) 이다. 이는 행렬-스타 그래프가 스타 그래프와 Macro-Star 그래프보다 망비용이 우수함을 의미한다.

• Korean Journal of Environmental Agriculture
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• v.34 no.2
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• pp.128-133
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• 2015

BACKGROUND: Plant extracts have been used as environment friendly agricultural materials for organic farming in South Korea. However safety evaluation on the plant extracts was not properly tested. The aim of this study was to evaluate safety of the extracts from Melia azedarach, Nerium indicum and Coptis chinensis on cultivating rice. METHODS AND RESULTS: Pant extarcts 300-fold diluted were treated on rice, and residues of M. azedarach, N. indicum and C. chinensis were determined. The analytes from the rice samples were detected by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method was validated, and good linearities ($r^2=0.995-0.998$), specificity, and recoveries were obtained. Limits of detection were 0.01 mg/kg for all of the target compounds. Recoveries were 79.3-118.3% at 0.1 mg/kg and 75.2-111.5% at 0.5 mg/kg. The residue levels were below 0.030 mg/kg for azadirachtin, 0.320 mg/kg for oleandrin and 1.460 mg/kg for berberine. CONCLUSION(S): The extracts of M. azedarach, N. indicum and C. chinensis contained azadirachtin, oleandrin and berberine as an active ingredient, respectively. The residue of three active ingredients dramatically decreased after treatment in all fruits, stems and roots of rice.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.37-38
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• 2004

Salmonella gallinarum is a pathogen that is capable of causing disease in Korean native chicken. Although Salmonella gallinarum is important world-wide pathogens of poultry, little is understood of the mechanisms of pathogenesis of Salmonella gallinarum in the chicken. This study was to investigate chicken liver proteins affected by infection of Salmonella gallinarum in Korean native chicken. The differentially expressed proteins of chicken livers were identified by using 2-dimensional electro- phoresis (2D-E) and mass spectrometry (MS). We detected more than 300 protein spots on silver stained 2D gels using pH 3∼10 gradients. Three differentially expressed protein spots were analyzed by MALDI-TOF MS and MS/MS. The obtained MS and MS/MS data were searched against a protein database using the Mascot search engine. Further researches on the identified proteins can give valuable information of mechanism of pathogenesis in chicken.