• 대한의용생체공학회:의공학회지
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• 제26권4호
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• pp.231-241
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• 2005

New methods to register multiple hemispheric slices of the postmortem brain to anatomically corresponding in-vivo MRI slices within a 3D volumetric MRI are presented. Gel-embedding and fiducial markers are used to reduce geometrical distortions in the postmortem brain volume. The registration algorithm relies on a recursive extraction of warped MRI slices from the reference MRI volume using a modified non-linear polynomial transformation until matching slices are found. Eight different voxel similarity measures are tested to get the best co-registration cost and the results show that combination of two different similarity measures shows the best performance. After validating the implementation and approach through simulation studies, the presented methods are applied to real data. The results demonstrate the feasibility and practicability of the presented co­registration methods, thus providing a means of MR signal analysis and histological examination of tissue lesions via co­registered images of postmortem brain slices and their corresponding MRI sections. With this approach, it is possible to investigate the pathology of a disease through both routinely acquired MRls and postmortem brain slices, thus improving the understanding of the pathological substrates and their progression.

• 대한견주관절학회:학술대회논문집
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• 대한견주관절학회 2009년도 제17차 학술대회
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• pp.175-175
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• 2009

Despite its importance for the understanding of joint kinematics in vivo, there has been few studies about shoulder joints. The purpose of this study is to analyze the glenohumeral joint during internal and external rotation at 90 degrees of abduction using in vivo noninvasive motion analysis system. MRI was performed for the following seven positions from maximum internal rotation to maximum external rotation at intervals of 30 degrees. We used 3D-gradient echo sequencing (TR: 12 ms, TE: 5.8 ms, 0.8 mm-slice thickness). Our method is based on matching three-dimensional MR images by the similarity of the image intensity. We analyzed the in vivo three-dimensional motions of the glenohumeral and scapulothoracic joint during this motion. In scapla plane, the mean rotation angle of the glenohumeral join was 105.5 degrees ($SD{\pm}39.0^{\circ}$). The mean rotation angle of the scapulothracic joint was 27.5 degrees ($SD\;{\pm}\;7.7^{\circ}$). The contribution ratio is almost 3.8:1 of glenohumeral and scapulothracic joint respectively.

• Molecules and Cells
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• 제23권2호
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• pp.132-137
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• 2007

With the advance of stem cell transplantation research, in vivo cell tracking techniques have become increasingly important in recent years. Magnetic resonance imaging (MRI) may provide a unique tool for non-invasive tracking of transplanted cells. Since the initial findings on the stem cell migration by MRI several years ago, there have been numerous studies using various animal models, notably in heart or brain disease models. In order to develop more reliable and clinically applicable methodologies, multiple aspects should be taken into consideration. In this review, we will summarize the current status and future perspectives of in vivo cell tracking technologies using MRI. In particular, use of different MR contrast agents and their detection methods using MRI will be described in much detail. In addition, various cell labeling methods to increase the sensitivity of signals will be extensively discussed. We will also review several key experiments, in which MRI techniques were utilized to detect the presence and/or migration of transplanted stem cells in various animal models. Finally, we will discuss the current problems and future directions of cell tracking methods using MRI.

• 한국정보통신학회논문지
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• 제15권10호
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• pp.2087-2092
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• 2011

본 논문에서는 지구 자기장을 측정하여 방향을 인식하는 시스템 구현에 있어 하드웨어적으로 회로를 부가하는 방식 보다는 소프트웨어적인 알고리즘을 추가하여 지구 자기장의 지역적 변화와 주변 환경에 적응력을 갖는 측정 시스템을 구축한다. 소프트웨어적인 알고리즘에는 뉴럴 네트워크(neural network)를 사용하여, 입력 패턴에 따른 패턴간의 관련성을 형성하고 학습을 통해 패턴들의 특징과 관련 정보가 기억 되었을 때 출력이 입력에 feedback하는 연상회로망을 구성하여 방향 인식에 사용할 수 있는 소프트웨어를 구현하고 그 실효성에 대해 입증한다.

• Bulletin of the Korean Chemical Society
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• 제27권2호
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• pp.211-218
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• 2006

This work deals with 24 substances composed of nitrogen-containing heterocycles. The relationships between the chromatographic retention factor (k) and those physicochemical properties which are relevant in quantitative structure-properties relationship (QSPR) studies, such as the polarizability $(\alpha)$, molar refractivity (MR), lipophilicity (logP), dipole moment $(\mu)$, total energy $(E_{tot})$, heat of formation $(\Delta H_f)$, molecular surface area $(S_M)$, and binding energy $(E_b)$, were investigated. The accuracy of the simple linear regressions between the chromatographic retention and the descriptors for all of the compounds was satisfactory (correlation coefficient, $0.8 \leq r \leq 1.0$). The QSPR models of these nitrogen-containing heterocyclic compounds could be predicted with a multiple linear regression equation having the statistical index, r = 1.000. This work demonstrated the successful application of the multiple linear approaches through the development of accurate predictive equations for retention factors in liquid chromatography.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.56-65
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• 1992

The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).

• 천문학회보
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• 제36권1호
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• pp.60.2-60.2
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• 2011

Galactic bars are supposed to be a channel of gas inflow to the galactic center and thus possibly help nuclear star-formation and AGN activities. However, previous studies based on small local samples did not agree with this expectation. We find it necessary to examine the expectation using a large sample and so investigate the effects of bar structures on galactic nuclear activities, based on the Sloan Digital Sky Survey (SDSS) DR7. We used 6,348 late-type galaxies brighter than Mr = -19.0 in the redshift range $0.01{\leq}z{\leq}0.05$. Late-type galaxies are visually classified into barred or unbarred galaxies using SDSS color composite images. We compare the fractions of galaxies showing star-formation and AGN activities among barred and unbarred galaxies as a function of optical color, stellar mass, and black-hole mass. We have found that bar enhances nuclear star-formation activity on galaxies having low stellar mass, and low black-hole mass. This effect is stronger in redder galaxies. In the case of AGN, bar effects are higher in intermediate-mass galaxies. Bars also have an effect on the strength(!) of the star-formation and AGN activity in our sample as well. Thus, it seems that nuclear activities are powered by gas inflow from galactic bar structures perhaps not always but under certain conditions.

• 한국공간구조학회논문집
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• 제19권2호
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• pp.43-50
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• 2019

A smart connective control system was invented recently for coupling control of adjacent buildings. Previous studies on this topic focused on development of control algorithm for the smart connective control system and design method of control device. Usually, a smart control devices are applied to building structures after structural design. However, because structural characteristics of building structure with control devices changes, a iterative design is required for optimal design. To defeat this problem, an integrated optimal design method for a smart connective control system and connected buildings was proposed. For this purpose, an artificial seismic load was generated for control performance evaluation of the smart coupling control system. 20-story and 12-story adjacent buildings were used as example structures and an MR (magnetorheological) damper was used as a smart control device to connect adjacent two buildings. NSGA-II was used for multi-objective integrated optimization of structure-smart control device. Numerical simulation results show the integrated optimal design method proposed in this study can provide various optimal designs for smart connective control system and connected buildings presenting good control performance.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제30권1호
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• pp.11-21
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• 2008

Aim of the study: Thermal stress is a central determinant of osseous surgical outcomes. Interestingly, the temperatures measured during endosseous surgeries coincide with the temperatures that elicit the heat shock response of mammalian cells. The heat shock response is a coordinated biochemical response that helps to protect cells from stresses of various forms. Several protective proteins, termed heat shock proteins (hsp) are produced as part of this response. To begin to understand the role of the stress response of osteoblasts during surgical manipulation of bone, the heat shock protein response was evaluated in osteoblastic cells. Materials & methods: With primary cell culture studies and ROS 17/2.8 osteoblastic cells transfected with hsp27 encoding vectors culture studies, the thermal stress response of mammalian osteoblastic cells was evaluated by immunohistochemistry and western blot analysis. Results: Immunocytochemistry indicated that hsp27 was present in unstressed osteoblastic cells, but not fibroblastic cells. Primarily cultured osteoblasts and fibroblasts expressed the major hsp in response to thermal stress, however, the small Mr hsp, hsp27 was shown to be a constitutive product only in osteoblasts. Creation of stable transformed osteoblastic cells expressing abundant hsp27 protein was used to demonstrate that hsp27 confers stress resistance to osteoblastic cells. Conclusions: The demonstrable presence and function of hsp27 in cultured bones and cells implicates this protein as a determinant of osteoblastic cell fate in vivo.

• 대한의용생체공학회:의공학회지
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• 제29권6호
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• pp.436-442
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• 2008

Magnetic resonance electrical impedance tomography(MREIT) has been suggested to produce cross-sectional conductivity images of an electrically conducting object such as the human body. In most previous studies, recessed electrodes have been used to inject imaging currents into the object. An MRI scanner was used to capture induced magnetic flux density data inside the object and a conductivity image reconstruction algorithm was applied to the data. This paper reports the performance of a thin and flexible carbon-hydrogel electrode that replaces the bulky and rigid recessed electrode in previous studies. The new carbon-hydrogel electrode produces a negligible amount of artifacts in MR and conductivity images and significantly simplifies the experimental procedure. We can fabricate the electrode in different shapes and sizes. Adding a layer of conductive adhesive, we can easily attach the electrode on an irregular surface with an excellent contact. Using a pair of carbon-hydrogel electrodes with a large contact area, we may inject an imaging current with increased amplitude primarily due to a reduced average current density underneath the electrodes. Before we apply the new electrode to a human subject, we evaluated its performance by conducting MREIT imaging experiments of five swine legs. Reconstructed conductivity images of the swine legs show a good contrast among different muscles and bones. We suggest a future study of human experiments using the carbon-hydrogel electrode following the guideline proposed in this paper.