Browse > Article

HSP27 EXPRESSION IN OSTEOBLAST BY THERMAL STRESS  

Rim, Jae-Suk (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Kim, Byeong-Ryol (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Kwon, Jong-Jin (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Jang, Hyon-Seok (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Lee, Eui-Suk (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Jun, Sang-Ho (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Woo, Hyeon-Il (Department of Oral & Maxillofacial Surgery College of Medicine, Korea University)
Publication Information
Maxillofacial Plastic and Reconstructive Surgery / v.30, no.1, 2008 , pp. 11-21 More about this Journal
Abstract
Aim of the study: Thermal stress is a central determinant of osseous surgical outcomes. Interestingly, the temperatures measured during endosseous surgeries coincide with the temperatures that elicit the heat shock response of mammalian cells. The heat shock response is a coordinated biochemical response that helps to protect cells from stresses of various forms. Several protective proteins, termed heat shock proteins (hsp) are produced as part of this response. To begin to understand the role of the stress response of osteoblasts during surgical manipulation of bone, the heat shock protein response was evaluated in osteoblastic cells. Materials & methods: With primary cell culture studies and ROS 17/2.8 osteoblastic cells transfected with hsp27 encoding vectors culture studies, the thermal stress response of mammalian osteoblastic cells was evaluated by immunohistochemistry and western blot analysis. Results: Immunocytochemistry indicated that hsp27 was present in unstressed osteoblastic cells, but not fibroblastic cells. Primarily cultured osteoblasts and fibroblasts expressed the major hsp in response to thermal stress, however, the small Mr hsp, hsp27 was shown to be a constitutive product only in osteoblasts. Creation of stable transformed osteoblastic cells expressing abundant hsp27 protein was used to demonstrate that hsp27 confers stress resistance to osteoblastic cells. Conclusions: The demonstrable presence and function of hsp27 in cultured bones and cells implicates this protein as a determinant of osteoblastic cell fate in vivo.
Keywords
Osteoblasts; Heat shock response; Heat shock protein 27; Osseointegration;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Branemark PI : Osseointegration and its experimental background. J Prosth Dent 50 : 399, 1983   DOI   ScienceOn
2 Adell R : The Surgical Principles of Osseointegration In Advanced Osseointegration Surgery: Applications in the Maxillofacial Region, Chicago, Quintessence Books, 1990, p.94
3 Eriksson RA, Albrektsson T : Temperature threshold levels for heat-induced bone tissue injury : A vital-microscopic study in the rabbit. J Prosth Dent 50 : 101, 1983   DOI   ScienceOn
4 Haider R, Watzek G, Plenk H : Effects of Drill Cooling and Bone Structure on IMZ Implant Fixation. Int J Oral Maxillofac Implants 8 : 83, 1993   PUBMED
5 Eriksson RA, Albrektsson T : The Effect of Heat on Bone Regeneration : An Experimental Study in the Rabbit Using the Bone Growth Chamber. J Oral Maxillofac Surg 42 : 705, 1984   DOI   ScienceOn
6 Lundskog J : Heat and bone tissue : An experimental investigation of the thermal properties of bone tissue and threshold levels for thermal injury. Scand J Plast Reconstr Surg 6 (suppl 9) : 5, 1972
7 Gething MJ, Sambrook J : Protein Folding in the Cell. Nature 355 : 33, 1992   DOI   ScienceOn
8 Uoshima K, Cooper LF : Rat hsp27 expression pattern indicates the comlex regulaton of hsp27 gene expression. Japan J Oral Biol 38 : 145, 1996   DOI
9 Puzas JE : Primer on the metabolic bone diseases and disorders of mineral metabolism, 3rd Ed. Philadelphia, Lippincott, 1993, p.11
10 Arrigo AP, Landry J : The Biology of Heat Shock Proteins and Molecular Chaperones, Cold Spring Harbor, Cold Spring Harbor Laboratory Press, 1994
11 Landry J, Chretien P, Lambert H et al : Heat shock resistance conferred by expression of the human HSP27 gene in rodent cells. J Cell Biol 109 : 7, 1989   DOI
12 Steller H : Mechanisms and genes of cellular suicide. Science 267 : 1445, 1995   DOI   ScienceOn
13 Mehlen P, Schulze OK, Arrigo AP : Small stress proteins as novel regulators of apoptosis. Heat shock protein 27 block Fas / APO-1 and starosporine - induced cell death. J Biol Chem 271 : 16510, 1996   DOI   ScienceOn
14 Lavoie JN, Grigas-Breton G, Tanguay RM et al : Induction of chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization. J Biol Chem 268 : 3420, 1993   PUBMED
15 Benndorf R, Haye K, Ryazantsev S et al : Phosphorylation and Supramolecular Organization of Murine Small Heat Shock Protein HSP25 Abolish Its Actin Polymerization - inhibiting Activity. J Biol Chem 269 : 20780, 1994
16 Chen CS, Mrksich M, Huang S et al : Geometric control of cell life and death. Science 276 : 1425, 1997   DOI   ScienceOn
17 Rothman JE : Polypeptide Chain Binding Proteins : Catalysts of Protein Folding and Related Processes in Cells. Cell 59 : 591, 1989   DOI   ScienceOn
18 Arata S, Hamaguchi S, Nose K : Effects of the overexpression of the small heat shock protein, hsp27, on the sensitivity of human fibroblast cells exposed to oxidative stress. J Cell Physiol 163 : 458, 1995   DOI   ScienceOn
19 O' Neal RB, Sauk JJ, Somerman MJ : Biological Requirements for Material Integration. J Oral Implantology 18 : 243, 1992
20 Rondeaux P, Galand P, Horman S et al : Effects of antisense hsp27 gene expression in osteosarcoma cells. In Vitro Cell Dev Biol - Animal 33 : 655, 1997   DOI   ScienceOn
21 Sato M and Rodan GA : Bone Cell Shape and Function, In Cell Shape : Determinants, Regulation and Regulatory Role, New York, Academic Press Inc, 1989, p.329
22 Picard D, Kursheed B, Garabedian MH et al : Reduced levels of hsp90 compromise steriod receptor action in vivo. Science 348 : 166, 1990
23 Klemenz R, Froehli E, Steiger RH et al : Alpha - B - crystallin is a small heat shock protein. Proc Natl Acad Sci USA 88 : 3653, 1991
24 Aubin JE, Kahn A : Primer on the metabolic bone diseases and disorders of mineral metabolism, 3rd Ed. Philadelphia, Lippincott, 1993, p.35
25 Samali A, Cotter TG : Heat shock proteins increase resistance to apoptosis. Exp Cell Res 223 : 163, 1996   DOI   ScienceOn
26 Eriksson RA, Adell R : Temperatures during Drilling for the Placement of Implants Using the Osseointegration Technique. J Oral Maxillofac Surg 44 : 4, 1986   DOI   ScienceOn
27 Welch WJ, Mizzen LA, Arrigo AP : Structure and Function of Mammalian Stress Proteins, New York, Alan R. Liss Inc, 1989, p187
28 Iwaki T, Iwaki A, Tateishi J et al : Sense and antisense modification of glial B-crystallin production results in alterations of stress fiber formation and thermoresistance. J Cell Biol 125 : 1385, 1994   DOI
29 Hochuli E, Dobeli H, Schacher A : New metal chelate adsorbents elective for proteins and peptides containing neighbouring histidine residues. J Chromatography 411 : 177, 1987   DOI   ScienceOn
30 Jakob U, Gaestel M, Engel K et al : Small heat shock proteins are molecular chaperones. J Biol Chem 268 : 1517, 1993   PUBMED
31 Friedenstein AJ : Osteogenic stem cells in the bone marrow. J Bone Miner Res 7 : 243, 1990
32 Towbin HT, Staehelin F, Gordon J : Electrophoretic Transfer of Proteins From Polyacrylamide Gels to Nitrocellulose Sheets : Procedures and Some Applications. Proc Natl Acad Sci USA 76 : 4350, 1979
33 Mehlen P, Briolay J, Smith L et al : Analysis of resistance to heat and hydrogen peroxide stresses in COS cells transiently expressing wild type or deletion mutants of the Drosophila 27-kDa heat shock protein. Eur J Biochem 215 : 277, 1993   DOI   ScienceOn
34 Shakoori AR, Oberdorf AM, Owen TA et al : Expression of Heat Shock Genes During Differentiation of Mammalian Osteoblasts and Promyelocytic Leukemia Cells. J Cell Biochem 48 : 277, 1992   DOI
35 Miron T, Vancompernolle K, Vandekerckhove J et al : A 25 kD inhibitor of actin polymerization is a low molecular mass heat shock protein. J Cell Biol 114 : 255, 1991   DOI
36 Zhu Y, Tassi L, Lane W et al : Specific Binding of the Transglutaminase, Platelet Factor XIII, to HSP27. J Biol Chem 269 : 22379, 1994
37 Klemenz R, Andres AC, Froehli E : Expression of the murine small heat shock proteins hsp25 and $\alpha$B-crystallin in the absense of stress. J Cell Biol 120 : 634, 1993
38 Riabowol KT, Mizzen LA, Welch WJ : Heat Shcok is Lethal to Fibroblasts Microinjected with Antibodies Against HSP70. Science 242 : 433, 1988   DOI
39 Bansal GS, Norton PM, Latchman DS : The 90-kDa Heat Shock Protein Protects Mammalian Cells from Thermal Stress but Not from Viral Infection. Exp Cell Res 195 : 303, 1991   DOI   ScienceOn