• Title/Summary/Keyword: MOC

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Analysis of Trans-Acting Elements for Regulation of moc Operons of pTi15955 in Agrobacterium tumefaciens

  • Jung, Won-Hee;Baek, Chang-Ho;Lee, Jeong-Kug;Kim, Kun-Soo
    • Journal of Microbiology and Biotechnology
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    • v.9 no.5
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    • pp.637-645
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    • 1999
  • Two putative regulator genes, mocR and mocS, of the moc (mannityl opine catabolism) operons in pTi15955 of the octopine-/mannityl opine-type Agrobacterium tumefaciens strain 15955, were tested for their possible roles as repressors in the moc operons. The regions upstream of macC and mocD, the first structural genes in the two divergently oriented moc operons, were transcriptionally fused into the promoterless lacZ reporter gene. Each of the lacZ-fusions was introduced into Agrobacterium strain UIA5, a Ti plasmid-cured derivative, harboring either a mocR or a mocS clone. The resulting strains were grown in media containing various sugar sources, and the $\beta$-galactosidase activities were quantitatively measured. The results suggested that MocR repressed the expression of macC and macD. The expression of the fused $\beta$-galactosidase was not induced by mannopine (MOP) or possible catabolic intermediates of the opine, e.g. santhopine (SOP), glucose, mannose, or glutamine. However, the repression was significantly relieved by the supplementation of MOP and the concomitant introduction of the agcA gene encoding MOP cyclase that catalyzes the lactonization of MOP to agropine (AGR). These results suggested that AGR, rather than MOP or the other catabolic intermediates, is the inducer for the expression of the operon. On the contrary to previous report showing that the induction levels of macC and macD were lowered by the supplementation of inorganic nitrogen in media, the expression of these genes was not affected by the level of nitrogen in our reporter system. MocS did not strongly repress the expressions of macC and mocD. It is possible that MocS may be involved in the regulation of the operons present downstream of the moc operon, which are responsible for the utilization of mannopinic acid and agropinic acid.

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Improvements of the CMFD acceleration capability of OpenMOC

  • Wu, Wenbin;Giudicelli, Guillaume;Smith, Kord;Forget, Benoit;Yao, Dong;Yu, Yingrui;Luo, Qi
    • Nuclear Engineering and Technology
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    • v.52 no.10
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    • pp.2162-2172
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    • 2020
  • Due to its computational efficiency and geometrical flexibility, the Method of Characteristics (MOC) has been widely used for light water reactor lattice physics analysis. Usually acceleration methods are necessary for MOC to achieve acceptable convergence on practical reactor physics problems. Among them, Coarse Mesh Finite Difference (CMFD) is very popular and can drastically reduce the number of transport iterations. In OpenMOC, CMFD acceleration was implemented but had the limitation of supporting only a uniform CMFD mesh, which would often lead to splitting MOC source regions, thus creating an unnecessary increase in computation and memory use. In this study, CMFD acceleration with a non-uniform Cartesian mesh is implemented into OpenMOC. We also propose a quadratic fit based CMFD prolongation method in the axial direction to further improve the acceleration when multiple MOC source regions are contained in one CMFD coarse mesh. Numerical results are presented to demonstrate the improvement of the CMFD acceleration capability in OpenMOC in terms of both efficiency and stability.

Effect of Graded Levels of Mustard Oil Cake Supplementation on Intake, Nutrient Digestibility, Microbial N Yield of Adult Cannulated Native (Bos Indicus) Bulls Fed Rice Straw

  • Chowdhury, S.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.5
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    • pp.715-722
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    • 1999
  • On a urea-molasses-straw (3:15:82, UMS) based diet, effect of graded levels of mustard oil cake (MOC) supplementation on the performances of native bulls has been studied. Four cannulated adult Bos indicus bulls of $415({\pm}44.6)kg$ live weight and 80 months old, were given daily either of 0, 200, 400 or 800 g of MOC in four periods in a $4{\times}4$ latin square design. Besides, each animal also received 200 g of each of molasses and wheat bran and a mineral mixture. For unit (1 g) increase in MOC intake, total DM intake increased by 0.8 g/d ($r^2=0.88$) but no change in the straw DM intake. With the increasing levels of MOC, crude protein (CP) digestibility increased exponentially with an asymptotic value of 72%. However, MOC level had no effect on the digestibilities of DM, OM and ADF. Similarly, rumen degradability of rice straw was also not affected by the level of dietary MOC, and mean straw DM degradabilities were 15, 21. 28, 37, 47 and 51% at 8, 16, 24, 48, 72 and 96 hours of incubation respectively. Microbial N yield per kg digestible organic matter apparently fermented in the rumen were 7.46, 8.77, 6.88 and 5.96 g respectively for 0, 200, 400 or 800 g of dietary MOC. For each gram increase in dietary MOC, N intake and N balance increased by 0.054 g/d ($r^2=0.998$) and $0.59mg\;N/kg\;W^{0.75}/d$ ($r^2=0.99$) respectively. Nitrogen balance was estimated to be attained at the N intakes of $246mg\;N/kg\;W^{0.75}/d$. Thus, on a UMS-based diet supplementation of MOC up to 800 g (10% of total intake) of the dietary intake had little or no effect on intake, digestibility, rumen parameters, and microbial N yield but slightly increased the N balance. However marginal response to MOC supplementation is probably due to the high degradability of MOC protein in the rumen. Thus, any substantial positive response of MOC supplementation on a UMS-based diet can probably be achieved by reducing its protein degradability in the rumen.

The Effects of Magnoliae officinalis Cortex and Machili thunbergii Cortex on Small Intestinal Motility (후박(厚朴)과 토후박(土厚朴)의 소장운동에 미치는 영향에 대한 연구)

  • Lee, Kyung-Jin;Park, Geun-Yong;Park, Gyu-Ha;Liu, Kwang-Hyeon;Kim, Tae-Wan;Ham, In-Hye;Bu, Young-Min;Choi, Ho-Young
    • The Korea Journal of Herbology
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    • v.26 no.4
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    • pp.75-81
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    • 2011
  • Objectives : Magnoliae officinalis Cortex (MOC) has been used in traditional medicine for digestive diseases in Korea, China and Japan. However, Machili thunbergii Cortex (MTC) also has been used as a substitute of MOC in Korea sometimes. Thus, this study was carried out to investigate and compare the effects of MOC and MTC on intestinal motility of isolated small intestinal segments from ICR mouse. Methods : Changes in motility were recorded via isometric transducers connected to a data acquisition system and amplitude, frequency and area under the curve (AUC) of intestinal spontaneous phasic contraction were compared. Results : The MOC extracts ($1{\sim}{\mu}g/mL$) dose-dependently decreased both amplitudes and frequencies of the spontaneous phasic contraction, but not AUC. However, high concentration of MOC (100 ${\mu}g$/mL) evoked tonic contraction. And it was not inhibited by tetrodotoxin, a sodium channel blocker, and nifedipine, a L-type $Ca^{2+}$ channel antagonist. These results suggested that MOC (100 ${\mu}g$/mL)-induced tonic contraction is not mediated by nerve or L-type $Ca^{2+}$ channel. On the other hand, the MTC extracts dose-dependently inhibited amplitude and AUC, but not the frequency. Conclusions : Although both MOC and MTC affected intestinal motility, MOC is more effective on intestinal motility than MTC. And MOC has been used as a traditional medicine for a long time but not MTC. Thus, we suggested that MTC should not be used in Korea as a substitute of MOC and MOC might be useful traditional medicine for gastrointestinal disease. The mechanism of MOC is still remained to elucidate.

Can Moringa oleifera Be Used as a Protein Supplement for Ruminants?

  • Kakengi, A.M.V.;Shem, M.N.;Sarwatt, S.V.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.42-47
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    • 2005
  • The possibility of using Moringa oleifera as a ruminant protein supplement was investigated by comparison between nutritive and anti-nutritive value of its different morphological parts with that of conventionally used Leucaena leucocephala leaf meal (LL). Parameters determined were chemical composition, rumen degradable protein (RDP), acid detergent insoluble protein (ADIP), pepsin soluble protein (PESP), non-protein nitrogen (NPN) total soluble protein (TSP) and protein potentially digested in the intestine (PDI). Total phenols (TP) and total extractable tannins (TET) were also evaluated as anti-nutritive factors. In vitro gas production characteristics were measured and organic matter digestibility (OMD) was estimated basing on 24 h-gas production. Crude protein content ranged from 265-308 g/kg DM in M. oleifera leaves (MOL) and seed cake (MOC) respectively. Leucaena leucocephala and Moringa oleifera soft twigs and leaves (MOLSTL) had CP content of 236 and 195 g/kg DM while Moringa oleifera soft twigs alone (MOST) and Moringa oleifera bucks (MOB) had 160, 114 and 69.3 g/kg DM respectively. RDP was highest in (MOC) (181 g/kg DM) followed by (MOL) (177 g/kg DM) and was lowest in MOB (40 g/kg DM). The proportion of the protein that was not available to the animal (ADIP) was (p<0.05) higher in MOL and MOC (72 and 73 g/kg DM) respectively and lowest in LL (29 g/kg DM). The PDI was high in LL (74 g/kg DM) followed by MOC (55 g/kg DM) then MOL (16 g/kg DM). PESP was highest (p<0.05) in MOC followed by MOL then LL (273, 200 and 163 g/kg DM respectively). MOC exhibited highest NPN content (116 g/kg DM) and was lowest in MOB (18 g/kg DM) (p<0.05). Highly (p<0.05) TSP was observed in MOC and MOL (308 and 265 g/kg DM respectively) followed by LL (236 g/kg DM). MOL had negligible TET (20 g/kg DM) when compared with about 70 g/kg DM in LL. Highly (p<0.05) b and a+b values were observed for MOLSTL (602 and 691 g/kg DM respectively) followed by MOL (490 and 538 g/kg DM). Highest c value was observed in MOSTL followed by MOC and MOL (0.064, 0.056 and 0.053 rate/hour) respectively. OMD was highest (p<0.05) for MOSTL followed by MOC and then MOL (579, 579 and 562 g/kg DM respectively). LL exhibited lower (p<0.05) OMD (467 g/kg DM). It was concluded from this study that the high crude protein content in MOL and MOLST could be well utilized by ruminant animals and increase animal performance however, high proportion of unavailable protein to the lower gut of animals and high rumen degradable protein due to negligible tannin content render it a relatively poor protein supplement for ruminants. MOC can be a best alternative protein supplement to leaves and leaves and soft twigs for ruminants.

Landscape Function and Tourism Industry: A Case Study of Moc Chau Plateau, Vietnam

  • LE, Hoa Thi Thu;TONG, Binh Thanh;VU, Ngoc Thi Minh;HO, Luu Si;PHAM, Thang Viet;TRINH, Hang Thi Thu
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.12
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    • pp.1195-1204
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    • 2020
  • Tourism is not only a cultural service but also a resource consuming industry. Landscape function framework is a powerful tool to show the relationship between nature and people. This study collects 50 documents around the world to analyze the relationship between the 4 functional groups of the landscape (regulatory functions, production and supply functions, resident functions, information and entertainment functions) and the tourism industry. On that basis, we created an establishment for practical contact analysis of the goods and services of the landscape for tourism development in Moc Chau plateau, Vietnam. Research results show that tourism is an economic sector that benefits from the landscape and has a clear resource orientation. Moc Chau plateau has rich tourism resources and has the conditions to develop various types of tourism, especially cultural tourism based on community and resort tourism. The two cultural functions and the providing functions are the two most important functional groups for the tourism development of Moc Chau district, bringing the two most important tourism icons for Moc Chau, a green steppe, cool milk benevolent and also a district rich in national culture. From these conclusions, the authors give recommendations and notable points about landscapes in the tourism industry, especially in places with topography like Moc Chau.

Prediction of Minimum Oxygen Concentration(MOC) of Hydrocarbons and Halogenated Hydrocarbons (탄화수소 및 할로겐화탄화수소의 최소산소농도(MOC)의 예측)

  • Ha Dong-Myeong;Jeong Kee-Sin
    • Fire Science and Engineering
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    • v.19 no.2 s.58
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    • pp.1-7
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    • 2005
  • An accurate knowledge of the minimum oxygen concentration(MOC) is important in developing appropriate prevention and control measures in industrial fire protection. In this study, by using the literature data and RSM(response surface methodology), the new equations for predicting the MOC are proposed. The A.A.P.E.(average absolute percent error) and the A.A.D.(average absolute deviation) of the reported and the calculated MOC for hydrocarbons were $3.48\%\;and\;0.37\;vol\%$, respectively and the correlation coefficient was 0.919. The A.A.P.E and the A.A.D of the reported and the calculated MOC for halogenated hydrocarbons and hydrocarbons were $5.06\%$ and $0.59vo1\%$, and the correlation coefficient was 0.938. The values calculated by the proposed equations were in good agreement with the literature data. Therefore, it is expected that this proposed equations will support the use of the research for other flammable substances.

Boundary Layer Correction of Hypersonic Wind-tunnel Nozzle Designed by the Methods of Characteristics (특성곡선 해법 설계 극초음속 노즐의 경계층 보정)

  • Kim, So-Yeon;Kim, Sung Don;Jeung, In-Seuck;Lee, Jong-Kuk;Choi, Jeong-Yol
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.42 no.12
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    • pp.1028-1036
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    • 2014
  • A design procedure is established for hypersonic nozzles by using MOC(Method of Characteristics) and CFD. The inviscid nozzle contour is designed by MOC, then BLC(Boundary Layer Correction) is made by evaluating the boundary layer thickness from viscous CFD analysis. By comparing various definitions of the boundary layer thicknesses, it seems that the boundary layer thickness of 95% speed of the maximum value at the cross section satisfies best the design Mach number. Design procedure is as follow; MOC design, grid generation, inviscid analysis, viscous analysis, BLC and viscous analysis for confirmation and post-processing. All procedures are made automatically by using the batch processing.

Management of Change in the Chemical Industries Based on Risk Assessment (위험성 평가에 기반한 화학산업의 변경관리)

  • Yoo, Jin Hwan;Lee, Heon Seok;Choi, Jeong Woo;Seo, Jae Min;Ko, Jae Wook
    • Korean Chemical Engineering Research
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    • v.46 no.1
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    • pp.156-163
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    • 2008
  • Process change and modification, which are caused by process failure, equipment life cycle and economic environment, have been generated in the chemical plants. And the MOC (management of change) base on the reasonable process safety technology such as change judgment, hazard identification by accurate technical references and risk assessment. But it is difficult to carry out the MOC because of lack of experience, shortage of knowledge and none of process safety specialists. In this study, the MOC system which could make enhancement of safety by finding and complementing weakness of MOC in chemical facilities was developed. This developed MOC system based on QRA was recommended the obvious standard for decision-making process, MOC procedure based on risk assessment and risk estimation of the process modification. The study based on the above way sought the enhancement of safety by performing Risk Based MOC for chemical plants.

Area based image matching with MOC-NA imagery (MOC-NA 영상의 영역기준 영상정합)

  • Youn, Jun-Hee;Park, Choung-Hwan
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.28 no.4
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    • pp.463-469
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    • 2010
  • Since MOLA(Mars Orbiter Laser Altimeter) data, which provides altimetry data for Mars, does not cover the whole Mars area, image matching with MOC imagery should be implemented for the generation of DEM. However, automatic image matching is difficult because of insufficient features and low contrast. In this paper, we present the area based semi-automatic image matching algorithm with MOC-NA(Mars Orbiter Camera ? Narrow Angle) imagery. To accomplish this, seed points describing conjugate points are manually added for the stereo imagery, and interesting points are automatically produced by using such seed points. Produced interesting points being used as initial conjugate points, area based image matching is implemented. For the points which fail to match, the locations of initial conjugate points are recalculated by using matched six points and image matching process is re-implemented. The quality assessment by reversing the role of target and search image shows 97.5 % of points were laid within one pixel absolute difference.