• Korean Journal of Fisheries and Aquatic Sciences
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• v.28 no.4
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• pp.428-434
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• 1995

The purpose of this study is to develop a sanitary aquarium for the safety slices of raw fish by using U.V. light. Water re-circulating system was composed of two tanks. One of the tanks $(90\times45\times45cm\;in\;size),$ was used for rearing fish and the other $(90\times45\times45cm\;in\;size),$ with 37 pieces of corrugated plastic plates was used for the growth of Nitrosomonas and Nitrobacter to remove ammonia from the water. Consequently, bactericidal effects of U.V. light were examined under the controlled condition of water with flow rate 730m1/sec (water flow thickness: 10mm), the width 41cm of water flow route, and the distance 4.75cm from the lamp to its water bottom, and U.V. light 75W (5 lamps). The water of the aquarium tank will be theroetically circulated 1 cycle per 18 min. In these conditions the bactericidal effect was $85\%$ just after passing through U,V. light and 3 log cycle in aquarium tank water. The count of Vibrio parahaemolyticus just after irradiation was decreased by about over than 3 log cycle. Under the irradiation for 72 hours, viable cell counts in both skin and gill of fish reared were decreased into about 2 log cycle, but there was no significant decrease in viscera. When the temperature of the tank was controlled at about $20-23^{\circ}C$ under the same condition, viable cell counts were reduced about 2 log cycle, and fecal coliforms were reduced about 1 log rycle and 3 log cycle in Crassostrea gigas and Mytilus edulis, respectively.

• Journal of Life Science
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• v.19 no.11
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• pp.1514-1521
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• 2009

To evaluate the feasibility of cathepsin-B levels in preoperatively screening patients with thyroid cancer, we assigned these patients to either the thyroid cancer group (n=32) or the nodular hyperplasia group (n=7). Five healthy volunteers served as controls (n=5). We quantified cathepsin-B expressions in cancerous lesions with follicular carcinoma and hyperplastic lesions with nodular hyperplasia, and compared the degrees to those of normal thyroid tissue, which was obtained from matched contralateral lobe. The activity of serum cathepsin B was significantly higher in patients with thyroid carcinoma ($284.87{\pm}79.32$, ${\times}10^{-2}\;mU$) and those with nodular hyperplasia ($255.45{\pm}95.68$, ${\times}10^{-2}\;mU$) than compared to normal control ($168.94{\pm}15.10$, ${\times}10^{-2}\;mU$) (p<0.05). Based on the results of immunoassay, the concentrations of cathepsin B in the thyroid cancer group ($15.50{\pm}7.86\;ng/ml$) and the nodular hyperplasia group ($17.64{\pm}7.49\;ng/ml$) were higher than those of the control group ($4.85{\pm}0.61\;ng/ml$). The degree of cathepsin-B mRNA expression was significantly higher in cancerous or hyperplastic lesions than normal thyroid tissues from matched contralateral lobe with follicular carcinoma or non-neoplastic thyroid disease. Our results indicate that the activity of serum cathepsin B is a useful indicator in screening patients with nodular hyperplasia or neoplastic thyroid disease and it may be involved in the abnormal proliferation of cells.

• Journal of the Korean Society of Food Culture
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• v.22 no.5
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• pp.603-608
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• 2007

Optimal winterizing condition of com oil was cooling temperature of bleached oil from $105^{\circ}C$ to $40^{\circ}C$. Then, filtering it after keeping $1{\sim}2^{\circ}C$ by lowering the temperature gradually with treating perlite of 0.3%(w/w) amount about bleached oil and stirring. We could measure that triglyceride(TG) that extracted from lipid components from spent perlite(SP) obtained through filtration after winterizing by SACC method is major causing materials of clouding in com oil. The result of separating TG fraction by agentation TLC was that it classified into 4 kinds -U3, SU2, S2U, S3 type and the most were U3 type. From this, it's easy to identify cause of clouding in com oil is TG fraction and most of them form wax materials that can observed. The results were they kept clear appearance at $0^{\circ}C$ generally during 39.6 to 96.5 hours, especially the result of A sample that had the lowest temperature condition while they have some difference by condition of treating temperature.

• Development and Reproduction
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• v.22 no.4
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• pp.309-318
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• 2018

The present study was conducted to investigate the regulatory mechanism of plasminogen activators (PAs) activation by $17{\beta}$-estradiol ($E_2$) and progesterone ($P_4$) in porcine uterine epithelial cells (pUECs). pUECs were collected from porcine uterine horn and cultured at 80% confluence. Then, 0.1% (v/v) DMSO, 20 ng/mL $E_2$, and $P_4$ with or without fetal bovine serum (FBS) treated to cultured cells for 24 hours. The supernatants were used for measurement of PAs activity and expression of urokinase-type PA (uPA), tissue-type PA (tPA), uPA specific receptor (uPAR), and type-1 PA inhibitor (PAI-1) mRNA were analyzed by real-time PCR. The expression of PAs-related genes was not affect by steroid hormones in both of serum treatment groups. However, PAs activity was increased by treatment of $E_2$ compared to 0.1% DMSO treatment in serum-free group (p<0.05). Then, $E_2$ and $P_4$ were diluted with 0.002% (v/v) DMSO for reduction of its effect and treated to cultured cells without FBS. Only tPA mRNA was significantly increased by $E_2$ treatment (p<0.05). PAs activity was enhanced in $E_2$ treated group compared to control groups (p<0.05). These results indicate that serum-free condition is more proper to evaluate effect of steroid hormones and activation of PAs in pUECs was mainly regulated by estrogen. These regulation of PAs activation may be associated with uterine remodeling during pre-ovulatory phase in pigs, however, further studies are needed to investigate precise regulatory mechanism.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.16 no.2
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• pp.116-122
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• 2013

Purpose: Preterm infants on parenteral nutrition are at a relatively high risk for hypertriglyceridemia because they have immature lipoprotein lipase activity. The purpose of this study was to analyze the clinical factors affecting lipid metabolism in preterm infants receiving parenteral nutrition and to evaluate the influence of intravenous heparin on serum triglycerides to determine the adequate heparin dose to prevent hypertriglyceridemia in preterm infants. Methods: A single-center retrospective review was conducted among preterm infants receiving parenteral nutrition between January 2006 and February 2011. In 75 patients, 110 determinations were performed within 28 days postnatal age. Demographic and clinical data, including laboratory parameters, the dose and the duration of lipid administration, and the amount of intravenous heparin, were analyzed. Results: Serum triglycerides were higher in the small for gestational age (SGA) infants than in the appropriate for gestational age infants ($185.5{\pm}134.9$ mg/dL vs. $126.9{\pm}101.9$ mg/dL, p=0.019). Birth weight, gestational age, and body weight were negatively correlated with serum triglyceride level (r=-0.289, p=0.002; r=-0.208, p=0.029; r=-0.287, p=0.002, respectively). The serum triglyceride level was statistically lower in preterm infants receiving 1 U/mL of heparin than in those receiving 0.5 U/mL heparin or no heparin. Conclusion: Preterm infants receiving parenteral nutrition, particularly SGA and extremely low birth weight infants, tend to have hypertriglyceridemia. Thus, administration of 1 U/mL of heparin rather than 0.5 U/mL or none may be helpful to prevent hypertriglyceridemia in preterm infants.

• Food Science of Animal Resources
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• v.23 no.2
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• pp.150-154
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• 2003

Mare milk has a unique composition compared to other animal milks. This study was to determine the content of vitamin A in mare milk using HPLC and compared with cow milk The RT(retention time) of vitamin A by HPLC was about 4.4 min in mare and cow milk The results showed that vitamin A content of cow milk was higher than that of mare milk in each gram of milk sample. And the vitamin A content of mare cream was lower than that of cow cream in each gram of lipid. Consequently, vitamin A content of cow milk was higher than that of mare milk.

• Journal of the Korean Dietetic Association
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• v.6 no.2
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• pp.108-116
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• 2000

The purpose of this study was to investigate the relationship among calcium intake, blood parameters related with bone metabolism, and serum lipids in healthy adults on self-selected diet. Subjects were consisted of 40 female college students residing in Chungnam. Anthropometric measurements, dietary intake measurements and blood collection were conducted. Serum concentrations of total protein, albumin, alkaline phosphates, leucine amino peptidase, BUN, calcium, inorganic phosphorus, and lipids were measured by biochemical analyzer and ICP spectrometer. The results were as follows. The mean age of subjects was 22.34 years and weight, height and BMI were 52.89kg, 161.29cm and 20.34, respectively. The daily mean energy and calcium intakes were 81.75% and 64.38% of RDA. The mean animal 1:2. The mean serum concentrations were 6.54g/dl(total protein), 4.12g/dl(albumin), 123.24U/(alkaline phosphates), 36.59U/l(leucine amino peptidase), 8.26mg/dl(calcium), 3.29mg/dl(inorganic phosphorus), 60.73mg/dl(triglyceride), 138.49mg/dl(total cholesterol), 65.95mg/dl(HDL-cholesterol), and 60.39mg/dl(LDL-cholesterol). There were no significant differences among calcium intake, bone metabolism parameters, and serum lipids when analyzed by Pearson's correlation coefficient. More systematic studies are required to investigate the roles of calcium in healthy persons on self-selected diets containing different levels of calcium.

• Journal of the korean academy of Pediatric Dentistry
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• v.29 no.3
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• pp.397-406
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• 2002

The purpose of this study was to clarify the palatal arch length, width and volume in the primary and permanent dentition. Samples were consisted of normal occlusion in the primary dentition(50 males and 50 females) and permanent dentition(43 males and 43 females). Their upper plaster casts were used and through 3-dimensional laser scanning(3D Scanner, DS4060, LDI, U.S.A.), cloud data, polygonization, section curve, loft surface and fit and horizontal plane were made for measuring the palatal arch length, width and volume(Surfacer 10.0, Imageware, U.S.A.). Correlation coefficients were calculated separately for males and females in each group(SPSS 10.0). The results were as follows : 1. Average distance from the fit plane to the points(tooth-tooth-palate) was greater in the permanent dentition than those of primary dentition. 2. Palatal volume was greater more than 3 times in the permanent dentition, especially it was greater in male compared to female with significance(p<0.05). 3. Palatal width of male was greater in the primary and permanent dentition but palatal length, only in the permanent dentition than that of female(P<0.05). 4. Correlation coefficients were statistically most significant between the palatal volume and size of posterior palatal width and total palatal length(r=0.401, r=0.450, r=0.678, r=0.654).

• Applied Biological Chemistry
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• v.27 no.2
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• pp.73-78
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• 1984

The optimum culture time and initial pH, for the production of exo-maltotetraohydrolase from Pseudomonas stutzeri IAM 12097, in the trypticase medium were 36 hrs and pH 6.3, respectively. Exo-maltotetraohydrolase was purified by $(NH_4)_{2}SO_4$ and two times of column chromatography on DEAE-cellulose. Specific activity of the purified enzyme was 108.6U/mg protein and yield of the enzyme activity was 9.4%. The purified enzyme showed a single band on polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis.

• Proceedings of the Materials Research Society of Korea Conference
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• 2012.05a
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• pp.69.2-69.2
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• 2012

Graphene films have a strong potential to replace indium tin oxide anodes in organic light-emitting diodes (OLEDs), to date. However, the luminous efficiency of OLEDs with graphene anodes has been limited by a lack of efficient methods to improve the low work function and reduce the sheet resistance of graphene films to the levels required for electrodes. Here, we fabricate flexible OLEDs by modifying the graphene anode to have a high work function and low sheet resistance, and thus achieve extremely high luminous power efficiencies (37.2 lm/W in fluorescent OLEDs, 102.7 lm/W in phosphorescent OLEDs), which are significantly higher than those of optimized devices with an indium tin oxide anode (24.1 lm/W in fluorescent OLEDs, 85.6 lm/W in phosphorescent OLEDs). We also fabricate flexible white OLED lighting devices using the graphene anode. These results demonstrate the great potential of graphene anodes for use in a wide variety of high-performance flexible organic optoelectronics.