• Asian-Australasian Journal of Animal Sciences
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• 제22권5호
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• pp.659-666
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• 2009

Metabolizable protein (MP) supply and amino acid balance in the intestine were manipulated through selection of highly digestible rumen-undegradable protein (RUP) sources and protected methionine (Met) supplementation. Four ruminallycannulated, multiparous Holstein cows averaging 193${\pm}$13 days in milk were used in a 4${\times}$4 Latin square design to assess N utilization and milk production responses to changes in RUP level, post-ruminal RUP digestibility and protected Met supplementation. Treatments were A) 14.0% crude protein (CP), 8.0% rumen degradable protein (RDP) and 6.0% RUP of low intestinal digestibility (HiRUP-LoDRUP); B) 14.1% CP, 8.1% RDP and 6.0% RUP of high intestinal digestibility (HiRUP-HiDRUP); C) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility (LoRUP-HiDRUP), and D) 13.1% CP, 7.9% RDP and 5.2% RUP of high intestinal digestibility plus rumen escape sources of Met (LoRUP-HiDRUP+Met). Experimental diets were formulated to have similar concentrations of RDP, net energy of lactation ($NE_L$), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium, phosphorus and ether extract using the NRC model (2001). Results showed that dry matter intake (DMI), production of milk fat and protein were similar among treatments. Milk production was similar for diet HiRUP-LoDRUP, HiRUP-HiDRUP and LoRUP-HiDRUP+Met, and significantly higher than diet LoRUP-HiDRUP. Milk fat and protein percentage were higher for cows receiving HiDRUP treatments, with the greatest increases in the diet LoRUP-HiDRUP+Met. There was no significant change in ruminal pH, $NH_3g-N$ and volatile fatty acid (VFA) concentration among all treatments. Apparent digestibility of dry matter (DM), CP, NDF and ADF and estimated bacterial CP synthesis were similar for all treatments. Nitrogen intakes, blood and milk urea-N concentrations were significantly higher for cows receiving HiRUP diets. Urine volume and total urinary N excretion were significantly lowered by LoRUP diets. Lowering dietary RUP level while supplementing the highly digestible RUP source with rumen escape sources of Met resulted in similar milk production, maximal milk fat and protein concentration and maximum N efficiency, indicating that post-ruminal digestibility of RUP and amino acid balance in the small intestine can be more important than total RUP supplementation.

• Asian-Australasian Journal of Animal Sciences
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• 제24권10호
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• pp.1393-1398
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• 2011

Four 4-month old Charolais${\times}$Dorset male sheep (initial liveweight $25.0{\pm}1.1\;kg$), fitted with rumen and abomasal fistulas and nourished by total intragastric infusions, were used to study the relationships between methionine (Met) supply, nitrogen (N) retention and plasma insulin-like growth factor-I (IGF-I). Four graded levels of Met, i.e. 0 g/16 g N, 1.76 g/16 g N, 3.52 g/16 g N and 7.04 g/16 g N, were infused into abomasums as experimental treatments. The sheep and treatments were allocated in a $4{\times}3$ incomplete Latin square design (Yudon square design). The experiment lasted 3 periods and each period was 10 days. Quadratic correlations were found between Met level (x, g/16 g N) and N retention (y, g/d): y = $-0.03x^2$+0.41x+2.62, $r^2$ = 0.66, n = 12, p = 0.008, and between methionine level (x, g/16 g N) and plasma IGF-I concentration (y, ng/ml): y = $0.80x^2$-4.53x+190.24, $r^2$ = 0.51, n = 12, p = 0.009. No significant correlation was found between plasma IGF-I (x, ng/ml) and N retention (y, g/d) (p>0.05). It was concluded that Met level had a significant influence on N retention and plasma IGF-I concentration whereas IGF-I might not be an important mediator in the regulation of N metabolism by Met in growing sheep nourished by total intragastric infusions.

• 사상체질의학회지
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• 제30권1호
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• pp.58-65
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• 2018

Objectives This study is to identify the relationship between stress, obesity and metabolic syndrome (MetS) by Sasang constitution (SC) and to use it as a basic data for the customized healthcare system of chronic disease management. Methods In a cross-sectional study conducted in a rural area of the Gyeongju area in 2014, we extracted data on 1,847 people using data from Korean medicine Data Center (KDC). The SC types diagnosed by Sasang constitutional medicine doctor. The stress was measured by the psychosocial well-being index questionnaire, and the obesity was defined by the body mass index. Results In all SC type, the stress and obesity was associated with MetS. There was a significant difference in the prevalence and trend of MetS according to presence of stress and obesity. The prevalence of MetS was the highest in Taeumin with all the stress and obesity, in order of Soeumin and Soyangin. Regardless SC types, the stress was associated with higher risk of MetS in the obese but not in the nonobese. Conclusion The results of this study indicate that further research is necessary to the SC types for the management of the Metabolic syndrome according to stress and obesity.

• Ecology and Resilient Infrastructure
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• 제7권1호
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• pp.43-52
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• 2020

본 연구는 컴퓨터 모델링의 결과를 실측자료 보정없이 이용하였을 때의 문제점을 조사하기 위해, 작은 도시공원을 대상으로 실측자료와 컴퓨터 모델링 결과를 비교 분석하여 보았다. 컴퓨터 모델링 결과는 실측자료에 비해 미기후 요소인 기온과 풍속에서는 과소예측을 보였으며, 상대습도와 평균복사온도에서는 과대예측을 하는 것으로 나타났다. 그 차이는 인간 열환경지수인 PMV와 UTCI에서 최대 2.3 PMV와 4℃ UTCI의 차이를 보였다. 그러므로, 인간 열환경지수 (열쾌적성) 연구에서 컴퓨터 모델링을 이용할 때는 반드시 실측자료를 이용한 결과보정 과정을 거쳐 도시와 조경계획 및 디자인에 적용되어야 할 것이다.

• 척추신경추나의학회지
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• 제6권1호
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• pp.75-88
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• 2011

후천성 회전형 사경으로 입원 치료한 환자 1례에 대하여 MET 기법을 위주로 한 한의학적 치료가 주소 증상에 유효한 효과가 있음을 확인하였다. 이에 저자는 본 증례를 보고하는 바이다.

• 대한통합의학회지
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• 제7권3호
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• pp.127-139
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• 2019

Purpose : The lipid profile is a major predictive index for cardiovascular disease, but there have only been a few studies conducted on the relationship between lipid ratio and cardiovascular disease risk factors in the Korean population. To address this research gap, we investigated the association between three lipid ratios and cardiovascular disease risk factors among Korean adults. Methods : This study used data from the seventh Korea National Health and Nutrition Examination Survey conducted in 2016. Lipid ratios included triglyceride (TG)/high density lipoprotein cholesterol (HDL), total cholesterol (TC)/HDL, and low density lipoprotein cholesterol (LDL)/HDL. Cardiovascular risk factors included in this research were: systolic blood pressure $(SBP){\geq}140$, diastolic blood pressure $(DBP){\geq}90$, fasting blood sugar $(FBS){\geq}126$, $HbA1c{\geq}6.5$, body mass index $(BMI){\geq}25$, waist circumference (WC) $men{\geq}90$, $women{\geq}85$, and metabolic syndrome (MetS). A complex samples logistic regression test was performed to analyze the association between lipid ratios and cardiovascular disease risk factors. Results : 1) TG/HDL ratio had statistically significant relationships with DBP, FBS, HbA1c, BMI, WC and MetS. 2) TC/HDL ratio was correlated to SBP, DBP, FBS, BMI, WC, and MetS. 3) LDL/HDL ratio had association with BMI, WC, and MetS. Conclusion : We identified significant association between lipid ratios and cardiovascular disease risk factors. The three lipid ratios were particularly strongly associated with BMI, WC, and MetS.

• Animal Bioscience
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• 제35권3호
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• pp.475-483
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• 2022

Objective: Methionine (Met) is involved in methyl group transfer besides protein synthesis. As the availability is limited and cost is high for synthetic Met, reductions in its inclusion in broiler diet may be possible by supplementing the low Met diets with methyl donors (MD) like betaine (Bet), folic acid (FA), vitamin B12 (B12), and biotin (Bio). An experiment was conducted to study the effects of supplementing the MD on performance (average daily gain [ADG], daily feed intake, feed efficiency [FE]), anti-oxidant variables, immune responses and serum protein concentration in broilers fed sub-optimal concentrations of dietary Met. Methods: Maize-soybean meal diet was used as control (CD). Different MD like Bet (0.2%), B12 (0.1 mg), FA (4 mg), or Bio (1.5 mg/kg) were supplemented to basal diet (BD) having no supplemental Met. The BD without MD was kept for comparison. Each diet was fed ad libitum to 10 replicates of 25 chicks in each from 1 to 42 d of age. Results: At the end of experiment, the ADG in MD group was higher than BD and lower than CD. The FE improved with FA or Bet compared to the BD. Breast meat weight was higher in Bet compared to the BD, while it was intermediate between BD and CD in other groups. The lipid peroxidation reduced with Bio, B12, or Bet, while the glutathione peroxidase activity improved with Bio or B12 compared to the BD. Lymphocyte proliferation improved with Bet compared to the BD. The serum protein concentrations increased with FA, Bio, or Bet compared to those fed BD. Conclusion: It can be concluded that the ADG can be improved partially with supplementation of MD while the FE improved with FA or Bet. Some MD also reduced the stress indices and improved immune responses compared to the BD fed broilers.

• Asian Pacific Journal of Cancer Prevention
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• 제16권6호
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• pp.2263-2268
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• 2015

Background: Many studies have reported associations of the X-ray repair cross-complementing group 3 (XRCC3) Thr241Met polymorphism with colorectal cancer (CRC) risk, but the results remained controversial. Hence, we performed the present meta-analysis with different inheritance models. Materials and Methods: We searched the PubMed and Google scholar databases for studies relating to associations between XRCC3 Thr241Met polymorphism and risk of CRC. 16 studies with 5,193 cases and 6,645 controls were finally included into the meta-analysis. Results: We found that the XRCC3 Thr241Met polymorphism was associated with increased CRC risk only under a dominant genetic model (CC+CT vs. TT: OR 0.575, 95%CI 0.498-1.665, p<0.001, $P_{heterogeneity}=0.00$, $I^2=83%$). There was a significant association between XRCC3 Thr241Met polymorphism and CRC risk in Caucasian in the overall 8 studies under only in the heterozygote genetic model (CT vs. TT: OR=0.929, 95%CI =0.806-1.070, P=0.308, $P_{heterogeneity}=0.002$, $I^2=57%$). Four studies evaluated the XRCC3 Thr241Met polymorphism and CRC risk in Asians. Two genetic models of the XRCC3 polymorphism were significantly correlated with increasing risk in Asians (dominant model: CC+CT vs. TT: OR= 0.609, 95%CI=411-0.902, P=0.013, $P_{heterogeneity}=0.54$, $I^2=0.00%$; Allele model: C vs. T: OR=0.708, 95 %=CI 0.605-0.829, p=0.000, $P_{heterogeneity}=0.000$, $I^2=92%$). The sensitivity analysis suggested stability of this meta-analysis and no publication bias was detected. Conclusions: In conclusion, this meta-analysis indicates that XRCC3 Thr241Met shows an increased CRC risk, particularly in Asians rather than Caucasians.

• Applied Biological Chemistry
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• 제6권
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• pp.107-117
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• 1965

(1) 콩고오지 제조중(製造中) 생성(生成)되는 저급(低級) peptde의 종류(種類)를 구명(究明)하는 동시(同時)에 겸(兼)하여 Aspergillus soya protease의 작용(作用) specificity를 구명(究明)하기 위(爲)하여 콩고오지 제조중(製造中) 생성(生成)되는 저급(低級) peptide의 N-terminal 및 C-terminal을 동정(同定)하고 dipeptide 및 tripeptide에 대(對)하여서는 amino acid sequence를 결정(決定)하여 다음과 같은 결과(結果)를 얻었다. Gly, Glu. Ala. Ser. Glu. Ser. Ala. Val (Cys, Glu, Ser, Ala, Arg, Try, Leu or Ileu) Asp. Phe (His, Arg, Cys, Asp, Ser, Ala, Leu or Ileu) Glu. Ala (Cys, Gly, Met) Glu. Ala (Asp, Glu,) Gly. Met (Asp, Glu, Ala, Tyr, Leu or Ileu, Lys,) Gly. Leu or Ileu (His, Asp, Glu, Gly, Ser, Lys, Thr, Phe,) Cys. Gly (Asp, Tyr,) Glu. Pro (Asp, Glu, Ser, Gly, Thr, Ala, Val, Leu or Ileu) Try. Ser (Gly, Glu, Arg, Ala, Met, Leu or Ileu,) Asp. Met (Asp, Glu, Ala, Try, Pro, Leu or Ileu,) His Thr (Ser, Gly, Tyr, Pro, Leu or Ileu,) Glu. Gly (Asp, Ala, Ser, Glu,) Leu or Ileu (2) Aspergillus soya protease의 작용(作用) specificity는 비교적(比較的) 광범위(廣範圍)하며 carboxyl기(基)를 가진 amino acid가 acidic amino acid일 때 잘 작용(作用)하는 것으로 보인다.

• KSBB Journal
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• 제16권4호
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• pp.389-397
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• 2001

본 연구에서는 T. thiooxidans MET 균주를 이용하여 비소화 슬러지와 혐기성 소화슬러지로부터 중금속을 용출하여 제거하는데 영향을 미치는 슬러지 성상의 영향을 조사하였다. 슬러지 종류와 농도에 관계없이 슬러지로부터의 중금속 용출은 슬러지 용액의 pH에 의존하였다. 슬러지 용액의 pH가 3.0 이하일 때 효율적인 중금속 용출 효율을 얻을 수 있었으며, Zn > Cu > Cr의 순서로 용출이 진행되었다. 비소화 슬러지 및 혐기성 소화 슬러지간에 buffering capacity의 차이는 없었으나, 동일 고형물 농도에서 혐기성 소화 슬러지에서 T. thiooxidans MET의 황산화속도가 높기 때문에, 혐기성 소화 슬러지 용액의 pH 감소 속도가 비소화 슬러지보다 빨랐다. 그러나, pH 변화에 따른 슬러지 단위 질량당 중금속 용출량을 비교한 결과, 동일 pH 조건에서 혐기성 소화슬러지의 중금속 용출량은 비소화 슬러지에서의 중금속 용출량 보다 낮았다. 이는 혐기성 소화 슬러지와 비소화 슬러지에 함유되어 있는 불용성 금속화합물의 차이 때문으로 사료되었다. 슬러지 성상에 관계없이, 슬러지 고형물 농도가 증가할수록 (10~70 g/L) 중금속 용출 효율은 감소하였고, 최적 S$^{\circ}$/슬러지 고형물의 비율은 0.1이었다. 또한, T thiooxidans MET 균주를 이용한 슬러지로부터 중금속 용출 공정은 90% 이상의 살균 효과와 20% 정도의 슬러지 감량 효과가 있었다.