• Proceedings of the Korean Society of Computer Information Conference
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• 2014.07a
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• pp.201-202
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• 2014

본 논문에서는 3차원 공간을 시각적으로 표현이 가능한 MAYA의 MEL Script를 통해 미로 찾기 로봇을 3D시뮬레이션으로 구현하는 방법을 제안한다. MAYA에서는 생성된 개체에 대해 X-Y-Z 좌표정보를 적용하고 이 수치를 개체의 속성 값으로 제공한다. 이를 이용하여 랜덤한 미로 맵을 완성하기 위한 규칙으로 행렬을 생성하고 이 행렬의 인덱스와 값에 따라 X-Y-Z 좌표정보를 적용하여 개체를 생성하면 랜덤한 미로 맵이 완성된다. 그 후 길을 찾기 위한 규칙에 의해 이동하는 로봇의 X-Z좌표정보를 각 프레임 별로 저장하여 재생 시키면 미로 찾기 로봇 시뮬레이션을 눈으로 확인 가능하다. 사람이 직접 번거롭게 임의의 미로 맵 을 생성하지 않고 로봇이 없어도 간편하게 미로 찾기를 구현해 볼 수 있는 방법을 제시한다. 본 시뮬레이터는 미로 찾기 알고리즘을 테스트하는데 유용할 것이다.

• YAKHAK HOEJI
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• v.47 no.6
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• pp.345-351
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• 2003

Heptaplatin, cis-Malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]platinum(II), is a novel platinum-based antitumor agent with clinical potential against human stomach cancer and the 3rd generation of the cisplatin. This study was performed to study how cisplain, heptaplatin and sunpla which is a mixture of heptaplatin and mannitol (w: w=l : 2) affect cell viability of SK-MEL-28 human melanoma cell line. Heptaplatin ($IC_{50}$/; 95.35 $\mu$M) and sunpla ($IC_{50}$/; 10.95 11M) were less effect than cisplatin (IC $_{50}$; 10.92 $\mu$M) on the SK-MEL-28 cells. By cell cycle analysis using flow cytometry, it was identified that the cells were arrested at G2/M phase by cisplatin, heptaplatin and sunpla, and percentage of cell death group was increased according to increasing of time and concentration. These results suggest that cisplatin, heptaplatin and sunpla are a novel anticancer agent against human melanoma cell.l.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.36 no.7
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• pp.737-744
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• 2012

A mobile emission laboratory (MEL) was designed to measure the amount of traffic pollutants, with high temporal and spatial resolution under real conditions. Equipment for the gas-phase measurements of CO, NOx, $CO_2$, and THC and for the measurement of the number, concentration, and size distribution of fine and ultra-fine particles by an FMPS and CPC was placed in a minivan. The exhausts of different types of vehicles can be sampled by an MEL. This paper describes the technical details of the MEL and presents data from the experiment in which a car chases passenger vehicles fuelled by diesel and gasoline. The particle number concentration in the exhaust of the diesel vehicle was higher than that of the gasoline vehicle. However, the diesel vehicle with a DPF emitted fewer particles than the vehicle equipped with a gasoline direct injection engine, with particle diameters over 50 nm.

• Biomolecules & Therapeutics
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• v.22 no.3
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• pp.207-212
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• 2014

Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in a-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed a-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in a-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in a-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses a-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.

• Journal of Ginseng Research
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• v.23 no.3 s.55
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• pp.190-197
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• 1999

In this study, the molecular mechanism of the growth inhibitory effect of panaxynol was investigated in a human malignant melanoma cell line, SK-MEL-l. In the cell cycle analysis, panaxynol arrested cell cycle progression of SK-MEL-I at the G1 phase. Immunoblot analysis demonstrated that panaxynol increased $p21^{WAF1}$ and decreased cdc2 expression. Protein levels of pl6, p27, E2F-1, Rb, and p53 were not changed. Thus, the changes in expression levels of $p21^{WAF1}$ and cdc2 apparently mediate the cell cycle arrest caused by panaxynol. In addition, cycloheximide (CHX) partially reversed the growth inhibition by panaxynol, which suggested that new protein synthesis was required. On the other hand, LLnL, a proteasome inhibitor, increased antiproliferative effect of panaxynol. This may be due to stabilization of the protein(s) responsible for the growth inhibition such as $p21^{WAF1}$. In summary, these results demonstrate that panaxynol inhibits proliferation of SK-MEL-I by inducing cell cycle arrest at the G1 phase and the inhibitory effect is mediated by the increased level of $p21^{WAF1}$ as well as decreased cdc2 expression.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2010.04a
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• pp.293-295
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• 2010

• Korean Journal of Medicinal Crop Science
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• v.12 no.3
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• pp.255-261
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• 2004

The MeOH extracts from 21 species were tested for their cytotoxicity against SK-MEL-28 melanoma cell line and HaCat normal cell line in 5 g/ml by sulforrhodamine-B (SRB) method. Among them, the MeOH extract from Moutan Cortex Radicis showed the moderate activity with the growth rate of 74.3% in SK-MEL-28 cells and the high activity with the growth rate of 207.8% in HaCat cells. Activity-guided fractionation was performed and five compounds, paeonol (1), benzoylpaeoniflorin (2), benzoic acid (3), 2,5-dihydroxy-4-methoxy- acetophenone (4), paeonfliorin (5) were isolated from hexane and EtOAc fraction. The structures were established by physicochemical and spectrometric methods $(mp,\;UV,\;IR,\;^1H-NMR,\;^{13}C-NMR,\;spectram)$. All compounds were evaluated for their cytotoxicity, compound 4 showed the significant cytotoxic activity with $ED_50$ value of $5.92\;{\mu}g/ml$, but the other compounds no activity. These results suggest that compound 4 is a novel anticancer candidate against SK-MEL-28 melanoma cells.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.4
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• pp.409-416
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• 2011

A mobile emission laboratory (MEL) was designed to measure the amount of traffic pollutants with high temporal and spatial resolution under real conditions. Equipment for gas-phase measurements of quantity of CO, NOx, $CO_2$, and THC and for the measurement of the number density and size distribution of fine and ultra-fine particles by a FMPS and a CPC were placed in a mini-van. The exhaust of different type of vehicles can be sampled by MEL. This paper describes the construction and technical details of the MEL and presents data from the experiment in which a car chases city buses fuelled by diesel, CNG, and LPG. The diameters of most particles in the exhaust of the diesel city bus were less than 300 nm and most of the particles had a diameter of 30-60 nm. However, most particles in the exhaust of the CNG and LPG city buses were nanoparticles (diameter: less than 50 nm).

• Archives of Pharmacal Research
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• v.29 no.3
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• pp.224-234
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• 2006

We employed human SK-MEL-28 cells as a model system to identify cellular proteins that accompany N-(4-methyl)phenyl-O-(4-methoxy)phenyl-thionocarbamate (MMTC)-induced apoptosis based on a proteomic approach. Cell viability tests revealed that SK-MEL-28 skin cancer cells underwent more cell death than normal HaCaT cells in a dose-dependent manner after treatment with MMTC. Two-dimensional electrophoresis in conjunction with matrixassisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis or computer matching with a protein database further revealed that the MMTC-induced apoptosis is accompanied by increased levels of caspase-1, checkpoint suppressor-1, caspase-4, NF-kB inhibitor, AP-2, c-Jun-N-terminal kinase, melanoma inhibitor, granzyme K, G1/S specific cyclin D3, cystein rich protein, Ras-related protein Rab-37 or Ras-related protein Rab-13, and reduced levels of EMS (oncogene), ATP synthase, tyrosine-phosphatase, Cdc25c, 14-3-3 protein or specific structure of nuclear receptor. The migration suppressing effect of MMTC on SK-MEL-28 cell was tested. MMTC suppressed the metastasis of SK-MEL-8 cells. It was also identified that MMTC had little angiogenic effect because it did not suppress the proliferation of HUVEC cell line. These results suggest that MMTC is a novel chemotherapeutic and metastatic agents against the SK-MEL-28 human melanoma cell line.

• Journal of Internet Computing and Services
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• v.1 no.2
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• pp.49-59
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• 2000

The majority of work done to date on natural language processing has focused on analysis and understanding of language, thus natural language generation had been relatively less attention than understanding, And people even tends to regard natural language generation CIS a simple reverse process of language understanding, However, need for natural language generation is growing rapidly as application systems, especially multi-language machine translation systems on the web, natural language interface systems, natural language query systems need more complex messages to generate, In this paper, we propose an algorithm to generate more flexible and natural sentence using lexical functions of Igor Mel'uk (Mel'uk ＆ Zholkovsky, 1988) and systemic grammar.