• Korean Journal of Environment and Ecology
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• v.22 no.5
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• pp.530-535
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• 2008

This study was carried out to analyze the vegetation and soil characteristic, and ordination of Wasabia japonica population at Ulleung-Do. The Wasabia japonica population is distributed in altitudes of 440m to 570m at Seo-Myon and also in altitudes of 340m near Nari-valley. In the study sites, soil organic matter, nitrogen, available phosphorous, exchangeable potassium, exchangeable calcium, exchangeable magnesium concentration, cation exchange capacity, and soil pH were 15.01%, 0.48%, 44.19ppm, 2.00(me/100g), 7.19(me/100g), 4.44(me/100g), 22.55(me/100g), and 6.17, respectively. The Rumohra standishii dominant population was mainly found in high in soil nitrogen, organic matter, CEC, and exchangeable calcium concentration in comparison with Boehmeria spicata dominant population. The Boehmeria spicata dominant population was mainly found in high in soil pH.

• The korean journal of orthodontics
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• v.47 no.1
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• pp.50-58
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• 2017

Introduction: Identifying menton (Me) on posteroanterior cephalograms and three-dimensional (3D) cone-beam computed tomography (CBCT) images is difficult, because the midpoint of the symphyseal area is not identifiable after the mandibular symphysis fuses at an early age. The aim of this study was to evaluate the reliability of the identification of the genial tubercle (GT) in patients with mandibular asymmetry and to compare it with that of the traditional landmark, Me. Methods: The samples comprised 20 CBCT images of adults with mandibular asymmetry. Two examiners performed the identifications and measurements. Me and GT were marked, and the anteroposterior, vertical, and transverse distances to the three reference planes were measured on 3D-reconstructed CBCT images. The intra- and inter-examiner reliability of landmark identification of Me and GT were assessed using the intraclass correlation coefficient (ICC) and Bland-Altman plots. Results: The Me and GT landmarks showed excellent reliability ($ICC{\geq}0.993$) three-dimensionally. In the transverse evaluation, the ICC values of the GT (range, 0.997-0.999) tended to be slightly higher than those of Me (range, 0.993-0.996). In the Bland-Altman plots for the two separate assessments, Me showed a maximum error of 1.76 mm in the transverse direction, whereas the GT showed a maximum error of 0.96 mm in the 95% limit. Conclusions: Our results suggest that both Me and GT are clinically reliable and equally useful landmarks for the evaluation of mandibular asymmetry on CBCT images.

• Toxicological Research
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• v.25 no.4
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• pp.243-251
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• 2009

Present study was conducted to evaluate the anti oxidative activity of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidative damage. The antioxidative activity of methanolic (MeOH) extract of the Agrimonia pilosa-Ledeb leaves on non-lipid oxidation, including liposome oxidation, deoxyribose oxidation, protein oxidation, chelating activity against metal ions, scavenging activity against hydrogen peroxide, scavenging activity against hydroxyl radical and 2'-deoxyguanosine (2'-dG) oxidation were investigated. The MeOH extract of the Agrimonia pilosa-Ledeb leaves exhibited high anti oxidative activity in the liposome model system. Deoxyribose peroxidation was inhibited by the MeOH extract of the Agrimonia pilosa-Ledeb leaves and MeOH extract of the Agrimonia pilosa-Ledeb leaves provided remarkable protection against damage to deoxyribose. Protective effect of MeOH extracts of the Agrimonia pilosa-Ledeb leaves on protein damage was observed at $600{\mu}g$ level (82.05%). The MeOH extracts of the Agrimonia pilosa-Ledeb leaves at $300{\mu}g$ revealed metal binding ability (32.64%) for hydrogen peroxide. Furthermore, the oxidation of 2'-deoxyguanosine (2'-dG) to 8-hydroxy-2-deoxyguanosine (8-OH-2'dG) was inhibited by MeOH extracts of the Agrimonia pilosa-Ledeb leaves and scavenging activity for hydroxyl radical exhibited a remarkable effect. From the results in the present study on biological model systems, we concluded that MeOH extract of the Agrimonia pilosa-Ledeb leaves was effective in the protection of non-lipids against various oxidative model systems.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.05a
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• pp.187-187
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• 2003

Methylmercury (MeHg), one of the heavy metal compounds, can cause severe damage to the central nervous system in humans. Many reports have shown that MeHg is poisonous to human body through contaminated foods and has released into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established so far. In this study, two methods, cDNA Microarray and SSH, were performed to assess the expression profile against MeHg and to identify differentially expressed genes by MeHg in neuroblastoma cell line. TwinChip Human-8K (Digital Genomics) was used with total RNA from SH-SY5Y (human neuroblastoma cell line) treated with solvent (DMSO) and 6.25 uM (IC50) MeHg. And we performed forward and reverse SSH method on mRNA derived from SH-SY5Y treated with DMSO and MeHg (6.25 uM). Differentially expressed cDNA clones were sequenced and were screened by dot blot and ribonuclease protection assay to confirm that individual clones indeed represent differentially expressed genes. These sequences were identified by BLAST homology search to known genes or expressed sequence tags (ESTs). Analysis of these sequences may provide an insight into the biological effects of MeHg in the pathogenesis of neurodegenerative disease and a possibility to develop more efficient and exact monitoring system of heavy metals as environmental pollutants.

• Korean Journal of Optics and Photonics
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• v.29 no.2
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• pp.70-76
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• 2018

Comparison calibration equipment for infrared-radiation thermometers below $0^{\circ}C$ has been established, using a TRT2 (transfer radiation thermometer 2, HEITRONICS) as a transfer standard and an ME30 (Model: ME30, HEITRONICS) as a variabletemperature blackbody. The TRT2 was calibrated using three fixed points (Ice ($0.01^{\circ}C$), In ($156.5985^{\circ}C$), and Sn ($231.928^{\circ}C$)) and the Planckian Sakuma-Hattori equation, and including the interpolation and extrapolation errors at $-50^{\circ}C$ in the uncertainty. The pneumatic lid is installed upon opening of the ME30 and is opened for only 30 seconds for measuring the radiation temperature, which prevents formation of ice in the ME30 and also reduces the calibration time to half. The farther away from the $0{\sim}232^{\circ}C$ region, the larger the uncertainty of the comparison calibration equipment becomes. The expanded uncertainty of the comparison calibration equipment was estimated as 0.26 K at $-20^{\circ}C$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.3
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• pp.444-449
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• 2001

Comparative effects of gamma irradiation and methyl bromide (MeBr) fumigation on disinfestation and physicochemical attributes of mung bean were investigated. Insects in domestic mung bean were identified to be Collosobruchus chinensis Linne. Ina disinfestation test, MeBr showed 100% disinfestation effect on Larva and adult at 5th day after treatments, while irradiation dose above 3 kGy was effective for disinfesting all larva at that time. Adults were more sensitive to radiation than larva. Around 1 kGy was enough to control larva and adult in mung bean following 10 to 15 days of irradiation. In physicochemical properties of treated sampels, nitrogen solubility, TBA value, amino acid and fatty acid compositions were not different among the control, 2.5 kGy-irradiated and MeBr-fumigated samples. MeBr fumigation caused the decrease in lightness (Hunter's L value) and the increase in redness (a value) and yellowness (b value), thereby resulting in overall color difference (${\Delta}E$) in a noticeable level (NBS 2.61~2.94).

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.12
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• pp.1865-1873
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• 2007

An experiment was performed to evaluate the effects of dietary metabolizable energy (ME) and lysine on carcass characteristics and meat quality in Arbor Acres (AA) broilers from 1 to 56 days of age. A total of 2,970 1-d-old male broiler chicks were randomly allocated to nine dietary treatments (three ME levels in combination with three lysine levels), and dietary ME and lysine concentrations were formulated by varying corn, soybean meal, tallow, and L-lysine sulfate concentrations. Live body weight (BW), carcass weight (CW), dressing percent, breast muscle weight (BMW), yield of breast muscle, muscle color (CIE L*, a*, and b*), pH values 45 min and 24 h postmortem ($pH_{45}$, and $pH_{24}$), meat shear force value (SFV), and water loss rate (WLR) were evaluated. Results showed that live body weight and dressing percent increased (p<0.05) as dietary energy increased. Higher dietary lysine content improved breast muscle weight. Neither carcass weight nor yield of breast muscle was affected by dietary energy or lysine content. Higher ME increased the b* value (p = 0.067) and $pH_{24}$ value (p<0.05), whereas it decreased SFV (p<0.05) and WLR (p = 0.06). Only water loss rate was influenced (p<0.01) by dietary lysine, which was higher in broilers from the high lysine diet as compared to those from medium or low lysine diets. The $pH_{45}$ value and L* value of breast muscle were not affected by ME or lysine. Significant interaction of dietary ME and lysine was found on a* value of breast muscle. These results indicated that dietary ME and lysine had important effects on breast muscle growth and meat quality, however their effects were different. Different concentrations of dietary ME and lysine might be considered to improve meat quality.

• Journal of Korean Society of Forest Science
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• v.97 no.3
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• pp.296-304
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• 2008

This study was carried out to analyze vegetation and soil characteristic, and ordination of Stewartia koreana community. The Stewartia koreana community was classified into Acer mono subcommunity, Pinus densiflora subcommunity, Sapium japonicum subcommunity, and Stewartia koreana typical subcommunity. In the study sites, soil organic matter, nitrogen, available phosphorous, exchangeable K, exchangeable Ca, exchangeable Mg concentration, cation exchange capacity, and soil pH were ranged from 14.31~34.27%, 0.33~0.77%, 7.83~22.88(mg/kg), 0.23~1.07(me/100 g), 0.29~2.93(me/100 g), 0.33~1.49(me/100 g), 10.47~22.48(me/100 g), and 4.65~4.96, respectively. The area of the Acer mono subcommunity, found in highlands, showed that contained the highest organic substances, nitrogen, available phosphorous, and cation exchangeable capacity concentration among the communities. The low elevation regions where the Pinus densiflor subcommunity is formed, showed the lowest concentrations of exchangeable Ca, and Mg.

• BMB Reports
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• v.31 no.2
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• pp.189-195
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• 1998

Methylmercury (MeHg), which is widely distributed in the environment, is well known for both its acute and chronic poisoning effects on the human health; however, the precise biochemical mechanisms by which this compound elicits its toxicity in a cellular level are still poorly understood. To examine whether MeHg-induced liver injury involves activation of Phospholipase $A_2$ ($PLA_2$), the $PLA_2$ activity of control and MeHg-administrated livers was measured. MeHg stably enhanced a liver form of cytosolic $PLA_2$ activity, which exhibited several biochemical properties similar to those of the 100 kDa $cPLA_2$, except in its elution profile of a DEAE-5PW HPLC, and it migrated as a molecular weight of 80 kDa in Western blot analysis. This blotting analysis also indicated that the MeHg-induced enhancement of the activity could be due to the increase in the amount of the enzyme protein rather than a stable modification of the enzyme such as phosphorylation. Our data also showed the higher myeloperoxidase activity in MeHg-administrated liver than in the control, suggesting that this increase in the amounts of the 80 kDa $PLA_2$ and its activity may be resulted from infiltration of neutrophils into the liver during a hepatic injury process such as MeHg-induced inflammation. Taken together, these data suggest that MeHg-induced liver injury may be mediated by activation of the 80 kDa form of liver cytosolic $PLA_2$.

• Mycobiology
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• v.47 no.4
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• pp.494-505
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• 2019

In this study, the antioxidant, anti-xanthine oxidase, anti-melanogenic and anti-wrinkle effects of methanol (ME) and hot water (HE) extracts from the fruiting bodies of Phellinus vaninii were investigated. The 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging activity of 2.0 mg/mL HE (95.38%) was comparable to that of butylated hydroxytoluene (96.97%), the reference standard. The hydroxyl radical scavenging activities of ME (98.19%) and HE (97.55%) were higher than that of butylated hydroxytoluene (92.66%) at 2.0 mg/mL. Neither ME nor HE was cytotoxic to murine melanoma B16-F10 cells at 25-750 ㎍/mL. Although the xanthine oxidase (XO) inhibitory effects of ME and HE were significantly lower than that of allopurinol, the values were higher than 84 percent. The in vitro tyrosinase inhibitory activities of ME and HE were comparable to kojic acid at 2.0 mg/mL. The cellular tyrosinase and melanin synthetic activities of ME and HE on B16-F10 melanoma cells at 500 ㎍/mL were higher than arbutin, indicating that the inhibitory effects of arbutin on the tyrosinase and melanin synthesis were higher than those of ME and HE. The collagenase inhibitory activity of HE was comparable to EGCG at 2.0 mg/mL, however, the elastase inhibitory activity of ME and HE was lower than EGCG at the concentration tested. The study results demonstrated that the fruiting bodies of Ph. vaninii possessed good antioxidant, anti-xanthine oxidase, cell-free anti-tyrosinase, cellular anti-tyrosinase, anti-collagenase, and moderate anti-elastase activities, which might be used for the development of novel anti-gout, skin-whitening, and skin anti-wrinkle agents.