• Journal of Pharmaceutical Investigation
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• 제27권4호
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• pp.313-321
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• 1997

Ion exchange resin complexes of famotidine have been prepared by the reaction of famotidine solution with activated ion exchange resins. Complex formation efficiency between famotidine and ion exchange resin was about $80{\sim}90%$ in average, calculated by HPLC determination. Drug release characteristics from the resin complexes were evaluated by the modified percolation method. Famotidine release was dependent on the type of ion exchange resins. In the case of weakly acidic resin complexes, the cumulative released amount of famotidine was more than 90% for 1hr in pH 1.2 buffer solution. However, in the case of strongly acidic resin complexes, it was less than 5% for 3hr in the same medium. Strongly acidic resins revealed some advantages over weakly, acidic resins for overcoming instability of famotidine in gastric juice. In addition, strongly acidic resin complexes showed controlled release of famotidine in pH 6.8 buffer solution, showing the result of about 60 to 70% of drug release for 5hr. After oral administrations of famotidine-resin complexes to rats as dose of 40 mg equivalent/kg, the pharmacokinetic parameters of famotidine were obtained by model independent analysis and compared with those of famotidine solution or suspension. $C_{max}$ of famotidine-resin complex was lower than that of famotidine solution or suspension. MRT, MAT, and MDT of the complexes were greater than those of famotidine solution or suspension. From these results, it was expected that famotidine was released slowly from the complexes and absorbed continuously into systemic circulation. It was recognized that drug release from the complexes was the rate-limiting step in drug absorption, since there were close correlations between in vitro drug release and in vivo pharmacokinetic parameters.

• 한국응용곤충학회지
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• 제48권2호
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• pp.269-274
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• 2009

구더기 치료에 널리 이용되고 있는 무균 구리금파리 [Phaenicia (=Lucilia) sericata (Meigen)]를 보다 손쉽게 사육하기 위한 배지를 선발하고, 알과 유충의 저장조건을 실험하였다. 시판되고 있는 8가지의 미생물 배양용 선택배지에서 실험한 결과, 알의 부화율은 배지 간에 차이가 없었고, 유충의 생존율은 1, 2차 실험결과 blood agar(BA), sabouraud dextrose agar 및 brucella blood agar 배지에서 가장 높았다. BA 및 chocolate agar 배지에서 양의 혈액 함량을 20${\sim}$40%로 높일수록 유충의 생육이 좋았다. 알은 부화율의 저하 없이 $8^{\circ}C$에서는 12일까지 저장할 수 있었고, 3령 초기 유충은 생존율의 저하 없이 $6^{\circ}C$에서 15일까지 저장할 수 있었다.

• 한국응용과학기술학회지
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• 제22권4호
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• pp.339-348
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• 2005

On the analysis of triacylglycerol (TG) from the kernels of Acanthopanax sessiliflorus by reversed phase-HPLC, it was separated into three main fractions of PN 44, 46 and 48, according to partition number (PN). On the contrary, it could be clearly classified into seven fractions of SMM, MMM, SMD, MMD, SDD, MDD and MDT by silver ion-HPLC by the number of double bond in the acyl chains of TG species. But resolution of so-called critical pairs of TG molecular species such as molecular pairs of $P_eLL$ $[C_{18:1{\omega}12}/(C_{18:2{\omega}6)2}]$ and OLL $[C_{18:1{\omega}9}/(C_{18:2{\omega}6)2}]$ and OOL $[(C_{18:1{\omega}9)2}/C_{18:2{\omega}6]$, and $P_eP_eL$ $[(C_{18:2{\omega}12)2}/C_{18:1{\omega}6]$ was not achieved $(P_e;$ petroselinic acid, L; linoleic acid, O; oleic acid). On the other hand, TG extracted from Aralia continentalis kernels were also fractionated into seven groups of SSM, SMM, MMM, SMD, MMD, SDD and MDD (S; saturated acid, M; monoenoic acid, D; dienoic acid) by silver ion-HPLC, although it's were classified into three groups of PN 44, 46 and 48 by reversed phase-HPLC. The fractions of SMM, MMM, MMD and MDD were divided into two subfractions, respectively; the fractions of SMM, MMM, MMD and MDD were resolved into the subfraction of $PP_e/P_e$ and POO (critical pairs from each other), that of $P_e/P_e/P_e$ and OOO, that of $P_e/P_e/L$ and OOL, and that of $P_e/L/L$ and OLL.

• Journal of Pharmaceutical Investigation
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• 제24권2호
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• pp.85-94
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• 1994

Inclusion complexes of ketoconazole (KT) with ${\alpha}-$, ${\beta}-cyclodextrin$ (CD) and dimethyl-${\beta}-cyclodextrin$ $(DM{\beta}CD)$ in a molar ratio of 1:2 were prepared by freeze-drying and solvent evaporation methods. The interactions of KT with ${\alpha}-CD$, ${\beta}-CD$ and $DM{\beta}CD$ in aqueous solution and in solid state were investigated by solubility study, infrared (lR) spectroscopy and differential scanning calorimetry (DSC). The stability constant of $KT-DM{\beta}CD$ inclusion complex (lC) was found to be the largest among three inclusion complexes. Clear differences in IR spectra and DSC curves were observed between inclusion complexes and physical mixtures (PM) of KT-CDs. It was also shown by IR spectra and DSC curves that solvent evaporation method might be. superior to the freeze-drying method in preparing the inclusion complexes of KT-CDs. The dissolution rate of KT was markedly increased by inclusion complex formation with CDs in the buffer solution at pH 4.0 and pH 6.8. The mean dissolution time (MDT,min), which represents the rapidity of dissolution, was in the order of $KT-DM{\beta}CD$ IC (3.20) < $KT-{\beta}-CD$ IC (4.36) < $KT-{\alpha}-CD$ IC (6.99) < $KT-{\alpha}-CD$ PM (17.46)< $KT-{\beta}-CD$ PM (19.36) < $KT-{\beta}-CD$ PM (28.53). The dissolution rates of KT-CD ICsprepared by solvent evaporation method were faster than those of KT-CD ICs prepared by freeze-drying method.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2002년도 추계종합학술대회
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• pp.196-201
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• 2002

어업무선국에서는 연안조업을 목적으로 약 10톤 미만의 소형어선과의 통신망으로 27MHz대의 주파수를 사용하는 SSB통신에 의존해 왔었다. 소형선박의 척수가 늘어나고, 어업통신본부에서는 각 도서지방을 무인화 시킴으로서 소형선박의 입ㆍ출항 및 조업사항을 관장해야 하는 통제 기능이 약화되기 시작했고, 현용 통신방식의 문제점이 드러나기 시작하면서 새로운 통신방식의 도입이 필요하게 되었다. 이들 선박은 SSB보다는 셀룰라폰을 그들의 주 통신망으로 사용하고 있으며 입ㆍ출항 신고는 물론이고 위치보고도 제대로 하지 않고 있는 실정이다. 또한 소형어선들은 항만 내외에서 운항거리를 단축하기 위해 주항로를 횡단하는 등 선박사고가 빈번히 발생하므로, 이런 소형선박의 사고를 미연에 방지하고, 해난사고 발생시 신속한 조치를 취하기 위하여 어업무선국과 선박간의 지속적인 통신을 유지하는 것이 가장 좋은 방법이라는 것은 두말할 나위가 없을 것이다. 현재, 어업무선국과 소형어선간에 사용하고 있는 27MHz의 SSB(Single Side Band)통신방식으로는 통제가 불가능 할 뿐더러 적절한 시기에 적절한 정보를 제공할 수 없는 게 어업통신의 현실이다. 본 연구에서는 10톤 미만의 소형어선과 어업무선국간의 원활한 통신체제를 구축하여 어민의 생명과 재산을 보호함은 물론 긴급상황 발생시 효과적으로 대처하여 소형어선의 해난사고를 줄이기 위한 통신 체제로서 주파수공용방식(TRS: Trunk Radio System)의 도입을 제안하고 그 활용성을 입증하고자 한다.

• Spatial Information Research
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• 제7권2호
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• pp.209-221
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• 1999

자바언어로 구현된 GIS 공간정보분서엔진, GPS, 휴대전화를 이용한 무선 데이터 통신을 연계하여 AVL-GIS 시스템을 개발하였으며, 프로토 타입으로서 긴급 상황 관제 센터로 가정한 통합안전관리시스템(경찰, 119 , 소방서)제 적용, 구현하였다. 이동차량이 정확한 위치의 추적을 위하여 IDGPS(Inverted Differential GPS) 방법을 사용하였으며, 휴대전화를 이용하여 GIS 분석기능과 도로 데이터 및 이와 관련된 공간정보를 갖고 있는 중앙 관제시스템을 실시간으로 연결하여 위치정보 및 메시지를 송수신할 수 있도록 하였다. 본 연구에서 개발된 시스템은 기존의 AVL용 시스템보다 GIS 분석기능이 강화되어 있으며, 비교적 저렴한 가격으로 물류정보, 재난 및 안전관리, 버스나 택시와 같은 차량관리와 전자상거래에서 인터넷을 통한 화물의 위치 확인등과 같은 응용시스템을 구축할 수 있다.

• Journal of Pharmaceutical Investigation
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• 제26권2호
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• pp.71-82
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• 1996

This study has been undertaken to evaluate hydrophobic cyclodextrin(CD) derivatives as a sustained release carrier of azidothymidine(AZT), AZT, which has potent activity against AIDS and AIDS-related complex as thymidine analogue, has been reported that it has significant toxicity and short half life. Therefore, it is necessary to design sustained release oral dosage form to avoid undesirable side effects attributable to an excessive plasma concentration and to reduce the frequency of administration of AZT. Inclusion complexes of AZT with $acetyl-{\beta}-cyclodextrin\;(AC{\beta}CD)$ and $triacetyl-{\beta}-cyclodextrin(TA{\beta}CD)$ were prepared by solvent evaporation method. Interactions of AZT with CD were investigated by Differential Scanning Calorimetry(DSC) and Infrared Spectrophotometry(IR). The decreasing order of water solubilities of AZT and AZT-CD inclusion complexes were as follows; $AZT\;(27.873{\pm}0.015,mg/ml)\;>\;AZT-AC{\beta}CD\;(3.377{\pm}0.003)\;>\;AZT-TA{\beta}CD\;(2.528{\pm}0.001)$. Partition coefficients of $AZT-AC{\beta}CD\;and;\AZT-TA{\beta}CD$ inclusion complexes were increased by 1.27-fold, 1.54-fold in pH 1.2 and 1.32-fold, 1.47-fold in pH 6.8 in comparison with that of AZT. The mean dissolution time (MDT, min) which represents the rapidity of dissolution rate of AZT, $AZT-AC{\beta}CD,\;AZT-TA{\beta}CD$ were 5.12, 14.02 and 19.38 min in pH 1.2 and 2.52, 15.19 and 18.19 min in pH 6.8. AZT was very rapidly and completely dissolved in pH 1.2 and pH 6.8 within 5 minutes. But AZT-CD inclusion complexes showed the sustained release pattern in comparison with AZT alone. The simultaneous in situ nasal and jejunal recirculation study to compare the intrinsic absorptivity and the property of absorption sites revealed that the absorption of $AZT-TA{\beta}CD\;(N:35.35{\pm}1.08%,\;J:27.47{\pm}1.18%)$ was more than that of $AZT\;(N:16.89{\pm}2.25%,\;J:15.86{\pm}2.33%)$. The above results suggest that $TA{\beta}CD$ which is a hydrophobic cyclodextrin may serve as sustained release carrier with absorption enhancing effect.

• 대한의생명과학회지
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• 제16권4호
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• pp.229-238
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• 2010

Chicken Mx protein (cMx) induced interferon (IFN) is an antiviral protein to inhibit replication of RNA virus, particularly negative stranded RNA virus, through blockage of transfortation of viral RNA and proteins. In order to determine antiviral effects of cMx from different MHC haplotype chicken, we characterized cMx gene by studying on nucleotide sequencing, antiviral effects to Newcastle disease virus, VSV and MDV, and transcription activities. Three types of eMx genes (2,118 bp) were detected from the different MHC haplotype chickens [B19 (N), B15(F) and B21 (GSP)] chickens, which have showed different susceptible to Marek's disease (MD). Several amino acid substitutions were showed in the cMx. The amino acid 548 and 631 in the cMxs from N and F, chickens susceptible to MD, was Val and Asn which was important on antiviral effects, and showed in resistant cMx. Those in the cMx from GSP, chicken resistant to MD, were same that showed in susceptible cMx. Though every cMx transactivated the expression of the reporter gene, the transcription activation by resistant cMx from N and F was lower compared to that by susceptible cMx from GSP. The decease of the cell growth in the resistant cMx cloned cells was seen in comparison with another cMx clone cells. Replication of NDV and VSV was suppressed in the clones with resistant cMx from N and F. NMx258-transducted cells lack of antiviral effects, and NMx437 or NMx646-transducted cells was showed 60% of antiviral effects compared to NMx705. Mean death time (MDT) and hemaggutination (HA) titer to NDV was long and low in the eggs of N and F lines, but short and high in the egg of GSP line. Interestingly, strong suppression to NDV was observed in the clone with N-Mx and in the eggs of N line. However, the effects of Mx for replication of vvMDV1 have not been. Thus, resistant types of cMx, N- and F-Mx, have showed the anti-viral effects to only RNA virus including NDV and VSV, but not to DNA virus. Antiviral effects of cMx were required whole length of amino acid including Val and Asn in amino acid 548 and 631.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.311-311
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• 2017

The relationships between rice grain filling and air temperature of maturing period in paddy field were analyzed to evaluate the effects of climatic change on rice productivity in Korea. Data of air temperature of 31, 41 and 51 days after heading(DAH) for 11 years from 2002 to 2016 were collected and analyzed to determine the effects on rice yield and yield component related traits of Chucheogbyeo, popular cultivar in Gyeonggi province in Korea. As the results, ripening ratio was closely correlated with the mean of daily maximum temperature (DMAT $r=0.71^*$), the mean of daily temperature difference (DTD, $r=0.67^*$) of 41 DAH and DTD ($0.65^*$) of 51 DAH. Weight of 1,000 paddy rice grains was closely correlated with accumulated sunshine hours (ASH) of 31 ($r=0.84^{**}$), 41 ($r=0.75^{**}$), 51 ($r=0.72^*$) DAH. Brown rice grain weight recovery ratio was closely correlated with DTD ($r=0.76^{**}$) and ASH ($r=0.84^{**}$) of 31 DAH, DMAT ($r=0.75^{**}$, $r=0.79^{**}$), DTD ($r=0.79^{**}$, $r=0.77^{**}$) and ASH ($r=0.81^{**}$, $r=0.79^{**}$) of 41 and 51 DAH. Paddy rice yield was closely correlated with MDT ($r=-0.63^*$) of 31 DAH, mean of daily minimum temperature (DMIT, $r=-0.83^{**}$, $r=-0.70^*$), DTD ($r=0.71^*$, $r=0.62^*$) of 31 and 41 DAH. Brown rice yield was correlated closely with DMIT ($r=-0.86^{**}$, $r=-0.73^*$) and DTD ($r=0.80^{**}$, $r=0.72^*$) of 31, 41 DAH, and DTD ($r=0.69^*$) of 51 DAH. Milled rice yield was correlated closely with DMIT ($r=-86^{**}$, $r=-0.73^*$), DTD ($r=0.79^{**}$, $r=0.71^*$) of 31, 41 DAH, and DTD ($r=0.68^*$) of 51 DAH.

• Journal of Korean Neurosurgical Society
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• 제30권sup1호
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• pp.85-90
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• 2001

Objective : DREZotomy is effective for the treatment of deafferentation pain as a consequence of root avulsion, postparaplegic pain, posttraumatic syrinx, postherpetic neuralgia, spinal cord injury, and peripheral nerve injury. We performed microsurgical DREZotomy to the patients with deafferentation pain and relieved pain without any serious complication. The purpose of this study is to evaluate the usefulness of the microsurgical DREZotomy for deafferentation pain. Methods : We evaluated 4 patients with deafferntation pain who were intractable to medical therapy. Two of them were brachial plexus injury with root avulsion owing to trauma, one was axillary metastasis of the squamous cell carcinoma of the left forearm, and the last was anesthesia dolorosa after surgical treatment(MVD and rhizotomy) of trigeminal neuralgia. Preoperative evaluation was based on the neurologic examination, radiologic imaging, and electrophysiological study. In the case of anesthesia dolorosa, we produced two parallel lesions in cephalocaudal direction, 2mm in distance, from the C2 dorsal rootlet to the 5mm superior to the obex including nucleus caudalis, after suboccipital craniectomy and C1-2 laminectomy, with use of microelectrode. In the others, we confirmed lesion site with identification of the nerve root after hemilaminectomy. We performed arachnoid dissection along the posterolateral sulcus and made lesion with microsurgical knife and microelectrocoagulation, 2mm in depth, 2mm in distance, to the direction of 30-45 degrees in the medial portion of the Lissauer's tract and the most dorsal layers of the posterior horn at the one root level above and below the lesion. Results : Compared with preoperative state, microsurgical DREZotomy significantly diminished dosage of the drugs and relieved pain meaningfully. One patient showed tansient ipsilateral ataxia, but recovered soon. There was not any serious complication. Conclusion : It may be concluded that microsurgical DREZotomy is very useful and safe therapeutic modality for deafferentation pain, especially segmentally distributed intermittent or evoke pain. Complete preoperative evaluation and proper selection of the patients and lesion making device are needed to improve the result.