• Title/Summary/Keyword: MCM-C

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The culture conditions for the mycelial growth of Auricularia auricula-judae

  • Jo, Woo-Sik;Kim, Dong-Geun;Seok, Soon-Ja;Jung, Hee-Young;Park, Seung-Chun
    • Journal of Mushroom
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    • v.12 no.2
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    • pp.88-95
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    • 2014
  • Auricularia auricula-judae is an edible mushroom, which is known as wood ear, free ear, black ear mushroom, and free jelly fish. This study was carried out to obtain the basic information for mycelial culture conditions of Auricularia auriculajudae. According to colony diameter and mycelial density, the media for suitable mycelial growth were PDA and MCM. The optimum temperature for mycelial growth was $25{\sim}30^{\circ}C$. Carbon and nitrogen sources were mannose and malt extract, respectively. The optimum C/N ratio was in the range of 10 to 1 with 2% glucose. Other minor components for the optimal growth were thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and $MgSO_4{\cdot}7H_2O$ and $KH_2PO_4$ as mineral salts.

The Growth Characteristics of Pleurotus eryngii (큰느타리버섯의 자실체 생육특성)

  • Ryu, Jae-San;Kim, Min-Keun;Kwon, Jin-Hyeuk;Cho, Sook-Hyun;Kim, Nak-Ku;Rho, Chi-Wong;Lee, Chun-Hee;Ro, Hyeon-Su;Lee, Hyun-Sook
    • The Korean Journal of Mycology
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    • v.35 no.1
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    • pp.47-53
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    • 2007
  • In this study, we investigated the properties of incubation and growing of Pleurotus eryngii in addition to the mycological properties to use them as basic data for breeding. The speed of mycelial growth on the MCM was faster than on the PDA. The biomass in the PDB broth culture was higher than in the MCM and YMG broth culture. KNR2515 and KNR2516 required 19 days for growth of mycelia on commercial sawdust media. KNR2503 required 6.5 days and 15.3 days for pin-heading and harvesting, respectively. In morphological properties by the mushroom, the heights of KNR2312 and KNR2322 were 122.7 and 121.0 mm, respectively. The thickness of KNR2322 and KNR2513 were 39.8 mm and 31.3 mm, respectively. The weight of KNR2524's fruiting body was 36.3 g, which is good as wild strain. The quality of fruiting body of KNR2503 was 4.0 in comparison to the score 7 of commercially cultivated strains. KNR2512 had the darkest color of pileus with L value 43.6. The slow growing strains, KNR2511, KNR2513, and KNR2512 had the bright pileus with L value 80. In morphological characteristics, KNR2511, KNR2513, and KNR2515 had white lamellar and plane pileus. The three strains are supposed to be the same group and KNR2516 and KNR2518 appeared to be related to the group. The commercially cultivated strains had convex pileus, KNR2502, KNR2503, KNR2504, KNR2521, and KNR2525 had infundibuliform, and the other strains had plane pileus. Several strains were valuable for breeding, JNR2503 for growth rate, KNR2512 for pileus color, and KNR2312, KNR2322, KNR2503, and KNR2513 for the quality.

Computation of Ionic Conductivity at NASICON Solid Electrolytes (II) Effects of mid-Na Sites on Na1-Na2 Conduction Paths (NASICON 고체 전해질의 이온 전도도 계산 (II) Na1-Na2 전도 경로에 미치는 mid-Na의 영향)

  • 최진삼;서양곤;강은태
    • Journal of the Korean Ceramic Society
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    • v.32 no.11
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    • pp.1292-1300
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    • 1995
  • The ionic conductivity of NASICON solid electrolytes was simulated by using Monte Carlo Method (MCM). There were included two conduction paths: (1) Na1-Na2 and (2) Na1-Na2 including Na2-Na2. We assumed that mid-Na ions provde an additional driving force for Na mobile ions due to the interionic repulsion between Na1 and Na2 ions. The inflection point of vacancy availability factor, V has been shown at nearby x=2, the maximum mid-Na ions. The inflection point of vacancy availability factor, V has been shown at nearby x=2, the maximum mid-Na sites are occupied. The effective jump frequency factor, V has been shown at nearby x=2, the maximum mid-Na sites are occupied. The effective jump frequency factor, W increased rapidly with the composition at low temperature, but decreased at high temperature region. On Na1-Na2 conduction path, the minimum of charge correlation factor, fc and the maximum of $\sigma$T were appeared at x=2.0. this indicated that mid-Na ions affect on the high ionic conductivity behavior. At the whole range of NASICON composition, 1n $\sigma$T vs. 1/T* plots have been shown Arrhenius behavior but 1n (VWFc) vs. 1/T* have been shown the Arrhenius type tendency at x=2, which mid-Na is being the maximum. The results of MCM agreed with the experimental one when the chosen saddle point value was 6$\varepsilon$ : 3$\varepsilon$. Here the calculated characteristic parameter of materials, K and the phase transition temperature were -4.001$\times$103 and 178$^{\circ}C$ (1/T*=1.92, 1000/T=2.22), respectively.

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Role of MAPK Signaling Pathways in Regulating the Hydrophobin Cryparin in the Chestnut Blight Fungus Cryphonectria parasitica

  • So, Kum-Kang;Kim, Dae-Hyuk
    • Mycobiology
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    • v.45 no.4
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    • pp.362-369
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    • 2017
  • We assessed the regulation of cryparin, a class II hydrophobin, using three representative mitogen-activated protein kinase (MAPK) pathways in Cryphonectria parasitica. Mutation of the CpSlt2 gene, an ortholog of yeast SLT2 in the cell wall integrity (CWI) pathway, resulted in a dramatic decrease in cryparin production. Similarly, a mutant of the CpBck1 gene, a MAP kinase kinase kinase gene in the CWI pathway, showed decreased cryparin production. Additionally, mutation of the cpmk1 gene, an ortholog of yeast HOG1, showed decreased cryparin production. However, mutation of the cpmk2 gene, an ortholog of yeast Kss1/Fus3, showed increased cryparin production. The easy-wet phenotype and accumulation of the cryparin transcript in corresponding mutants were consistent with the cryparin production results. In silico analysis of the promoter region of the cryparin gene revealed the presence of binding motifs related to downstream transcription factors of CWI, HOG1, and pheromone responsive pathways including MADS-box- and Ste12-binding domains. Real-time reverse transcriptase PCR analyses indicated that both CpRlm1, an ortholog of yeast RLM1 in the CWI pathway, and cpst12, an ortholog of yeast STE12 in the mating pathway, showed significantly reduced transcription levels in the mutant strains showing lower cryparin production in C. prasitica. However, the transcription of CpMcm1, an ortholog of yeast MCM1, did not correlate with that of the mutant strains showing downregulation of cryparin. These results indicate that three representative MAPK pathways played a role in regulating cryparin production. However, regulation varied depending on the MAPK pathways: the CWI and HOG1 pathways were stimulatory, whereas the pheromone-responsive MAPK was repressive.

Selection of Superior Strains from Collected Ear Mushrooms for Artificial Cultivation and Their Optimal Condition of Mycelial Growth (목이류 인공재배를 위한 우량균주 선발 및 균사생장 최적 조건 구명)

  • Kim, Jeong-Han;Lee, Yun-Hae;Jang, Myoung-Jun;Won, Seon-Yi;Ju, Young-Cheoul
    • The Korean Journal of Mycology
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    • v.42 no.1
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    • pp.57-63
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    • 2014
  • This study was carried out to obtain morphological and physiological characteristics of ear mushrooms for an artificial cultivation. Eighteen strains were cultivated with bag culture and classified into mainly five groups such as brown, black, white, purple and others group. The highest yield was shown in 43007 strain as 98.3 g/bag and 43009, 43016, 43025 and 44035 strains were more than 60 g/bag. Among collected strains, 43007, 43009 and 43035 were selected in this study as superior strains. Three selected strains were investigated for optimal mycelial growth conditions. MCM and GPYM media were selected for the favorable culture medium. The carbon sources of 43007, 43009 and 43035 on mycelial growth were maltose, fructose and glucose, respectively and peptone was selected as a nitrogen source. Highest mycelial growth was observed when the C/N ratio was 10 for 43007 and 20 for 43009 and 43035.

Analysis of genetic characteristics and development of substrate for cultivation in brown strains of Flammulina velutipes (갈색팽이버섯의 유전적 특성검정 및 배지재료 개발)

  • Choi, Jae-Sun;Noh, Jae-Goan;Jang, Who-Bong;Choi, Seong-Yeol;Min, Kyoung-Beom;Kong, Won-Sik
    • Journal of Mushroom
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    • v.5 no.1
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    • pp.13-20
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    • 2007
  • Analysis of genetic characteristics of Flammulina velutipes showed that strains have a range of 85% in genetic distribution diagram. According to this result, we divided these strains into five groups. In experiment of determinating the optimum media and condition in cultivating F. velutipes, we found the optimum temperature and pH range for hypha growth were $25^{\circ}C$ and 6.0 to 7.0, respectively. in addition, the best media for growth of that in plate was MCM (Mushroom Complete Media) which have a growth length from 68 to 83 mm. In vivo test, we observed that fast growth and good density of hyphae in mixture media of douglas fir sawdust, cotton seed meal and beet pulp (6:2:2 V/V). Also when we cultivated F. velutipes in this media, we harvested high yield of fruiting body.

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Cultural Characteristics of Mycelial Growth by an Entomogenous Fungus, Cordyceps pruinosa Petch (붉은자루동충하초의 균사생육에 관한 연구)

  • Hong, In-Pyo;Nam, Sung-Hee;Jung, I-Yeon;Sung, Gyoo-Byung;Nam, Hack-Woo;Chang, Seung-Jong;Hyeon Hyur;Lee, Min-Woong;Guo, Shun-Xing
    • Journal of Sericultural and Entomological Science
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    • v.45 no.2
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    • pp.116-120
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    • 2003
  • This study was carried out to obtain basic data on physiological characteristics for an artificial cultivation of fruiting body of C. pruinosa. C. pruinosa showed the most favorable growth on the MCM medium. The optimal condition for the mycelial growth was obtained at 25$^{\circ}C$ and pH 5.0, respectively. Carbon sources such as arabinose, mannose, xylose were favorable for stimulating a mycelial growth and fruiting bodies of C. pruinosa. Ammonium nitrate, ammonium citrate and ammonium tartrate of nitrogen sources also appeared to be good in the mycelial growth and fruiting body formation.

LTCC and LTCC-M Technologies for Multichip Module (Multichip module 개발을 위한 LTCC 밀 LTCC-M 기술)

  • 박성대;강현규;박윤휘;문제도
    • Journal of the Microelectronics and Packaging Society
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    • v.6 no.3
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    • pp.25-35
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    • 1999
  • LTCC (Low Temperature Cofired Ceramic) or LTCC-M (Low Temperature Cofired ceramic on Metal) technology is one of MCM-C (Multichip Module on Ceramic) technologies and becomes to be widely used in consumer, RF and automotive electronics. As green sheets for LTCC are cofired below $1000^{\circ}C$ in comparison with those for HTCC (High Temperature Cofired Ceramic), high conductivity metal traces such as gold, silver and copper can be used. The dimensional stability in LTCC-M technology enables embedded passives to be integrated inside modules, which enhances the electrical performance and increases the reliability of the modules. Coefficient of thermal expansion and dielectric constant can be controlled by changing composition and heating profile for cofiring. In this technical review, LTCC and LTCC-M technologies are introduced and advantages of those technologies are explained.

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Culture Conditions for the Mycelial Growth of Ganoderma applanatum

  • Jo, Woo-Sik;Cho, Yun-Ju;Cho, Doo-Hyun;Park, So-Deuk;Yoo, Young-Bok;Seok, Soon-Ja
    • Mycobiology
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    • v.37 no.2
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    • pp.94-102
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    • 2009
  • Ganoderma applanatum is one of the most popular medicinal mushrooms due to the various biologically active components it produces. This study was conducted to obtain basic information regarding the mycelial culture conditions of Ganoderma applanatum. Based on the colony diameter and mycelial density, PDA, YMA and MCM media were suitable for the mycelial growth of the mushroom. The optimum temperature for mycelial growth was found to be $25{\sim}30^{\circ}C$. The optimum carbon and nitrogen sources were mannose and dextrin, respectively, and the optimum C/N ratio was 2 to 10 when 2% glucose was used. Other minor components required for the optimal growth included thiamine-HCl and biotin as vitamins, succinic acid and lactic acid as organic acids, and $MgSO_4$ $7H_2C$, $KH_2PO_4$ and NaCl as mineral salts.

3D IC Using through Silicon via Technologies (TSV 기술을 이용한 3D IC 개발 동향)

  • Choi, K.S.;Eom, Y.S.;Lim, B.O.;Bae, H.C.;Moon, J.T.
    • Electronics and Telecommunications Trends
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    • v.25 no.5
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    • pp.97-105
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    • 2010
  • 모바일과 유비쿼터스 센서 네트워크 센서 시대가 도래함에 따라 가볍고, 작고, 얇고, 멀티기능을 구현할 수 있는 부품에 대한 요구가 증대하고 있다. 이에 대한 여러 가지 솔루션 중 MCM의 개념을 수직 방향으로 확장시킨 3D IC가 최근 각광을 받고 있다. 이는 물리적인 한계에 부딪힌 반도체 집적 공정의 한계를 극복하여 지속적으로 무어의 법칙에 맞춰 집적도를 향상시킬 수 있을 뿐만 아니라 소재와 공정이 달라도 3차원적으로 집적이 가능하여 메모리와 프로세서로 대표되는 디지털 칩뿐만 아니라 아날로그/RF, 수동소자, 전력소자, 센서/액추에이터, 바이오칩 등을 하나로 패키징 할 수 있는 장점이 있기 때문이다. 이를 통해 성능 향상, 경박단소, 저비용의 부품 개발이 가능하기 때문에 미국, 유럽, 일본 등 선도국뿐만 아니라 싱가포르, 타이완, 중국 등에서도 활발한 연구가 진행되고 있으며 CMOS 이미지 센서 모듈 생산에 TSV 기술이 이미 적용되고 있다. 본 고에서는 3D IC를 위한 TSV 및 적층 요소 기술을 소개하고 이를 통해 개발된 사례와 표준화 동향에 대하여 소개하고자 한다.