• Title/Summary/Keyword: MCL1

Search Result 171, Processing Time 0.026 seconds

Pseudomonas oleovorans의 유가식 배양에 의한 medium chain length Polyhydroxyalkanoates (MCL-PHA) 생산

  • Kim, Beom-Su;Im, Hui-Yeon
    • 한국생물공학회:학술대회논문집
    • /
    • 2000.04a
    • /
    • pp.207-210
    • /
    • 2000
  • Pseudomonas oleovorans was cultivated to produce medium chain length polyhydroxyalkanoates (MCL-PHA) fram octanoic acid and ammonium nitrate as carbon and nitrogen source, respectively, by a pH-stat fed-batch culture technique. The octanoate concentration of the culture broth was maintained below 4 g/L by feeding the mixture of octanoic acid and ammonium nitrate when the culture pH rose above high limit. The effect of the ratio of octanoic acid to ammonium nitrate (C/N ratio) in the feed on the PHA production was examined. The final cell concentrations of 62.5, 54.7, and 9.5 g/L, PHA contents of 62.9, 75.1, and 67.6% of dry cell weight, and productivities of 1.03, 0.632, and 0.161 g/L/h were obtained when the C/N ratio in the feed were 10, 20, and 100 g octanoic acid/g ammonium nitrate, respectively.

  • PDF

Influences of Inter-syllable Pause Duration on Speech Discrimination Score in Children with Cochlear Implantation (음절 간 쉼 간격이 인공와우 아동의 어음이해도에 미치는 영향)

  • Park, J.I.;Heo, S.D.
    • Journal of rehabilitation welfare engineering & assistive technology
    • /
    • v.8 no.4
    • /
    • pp.245-250
    • /
    • 2014
  • The aims of this study was to investigate influences of speech discrimination score(SDS) depending on inter-syllable pause duration in participant with child of cochlear implantation(CI). 12 child of CI-user participated. The word for SDS was used self-made meaningless three-syllable. The pause duration of inter-syllable was adjusted to 250, 500, 1,000 millisecond(ms). Discrimination score of closed-set speech was obtained at most comfortable loudness(MCL). SDS were improved in CI group for 62.08, 63.75, 69.58 %, but there were no significant changes in child of CI group(p = .4635). SDS was improved depending on inter-syllable pause duration in child of CI.

  • PDF

MaoC Mediated Biosynthesis of Medium-chain-length Polyhydroxyalkanoates in Recombinant Escherichia coli from Fatty Acid (재조합 대장균에서 MaoC를 이용한 지방산으로부터의 중간사슬길이 폴리하이드록시알칸산 생산 연구)

  • Park, Si Jae;Lee, Seung Hwan;Oh, Young Hoon;Lee, Sang Yup
    • KSBB Journal
    • /
    • v.29 no.4
    • /
    • pp.244-249
    • /
    • 2014
  • Biosynthesis pathway of medium-chain-length (MCL) polyhydroxyalkanoates (PHA) from fatty acid ${\beta}$-oxidation pathway was constructed in recombinant Escherichia coli by introducing the Pseudomonas sp. 61-3 PHA synthase gene (phaC2) and the maoC genes from Pseudomonas putida, Sinorhizobium meliloti, and Ralstonia eutropha. The metabolic link between fatty acid ${\beta}$-oxidation pathway and PHA biosynthesis pathway was constructed by MaoC, which is homologous to P. aeruginosa (R)-specific enoyl-CoA hydratase (PhaJ1). When the E. coli W3110 strains expressing the phaC2 gene and one of the maoC genes from P. putida, Sinorhizobium meliloti, and Ralstonia eutropha were cultured in LB medium containing 2 g/L of sodium decanoate as a carbon source, MCL-PHA that mainly consists of 3-hydroxyhexanoate (3HHx), 3-hydroxyoctanoate (3HO) and 3-hydroxydecanoate (3HD), was produced. The monomer composition of PHA and PHA contents varied depending on MaoC employed for the production of PHA. The highest PHA content of 18.7 wt% was achieved in recombinant E. coli W3110 expressing the phaC2 gene and the P. putida maoC gene. These results suggest that MCL-PHA biosynthesis pathway can be constructed in recombinant E. coli strains from the b-oxidation pathway by employing MaoC able to supply (R)-3-hydroxyacyl-CoA, the substrate of PHA synthase.

The Interference Effect of DTV Service on Radio Microphone (무선 마이크에 대한 DTV 서비스 간섭 영향 분석)

  • Shim, Yong-Sup;Lee, Il-Kyoo;Cheng, Yan-Ming
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.10 no.2
    • /
    • pp.19-24
    • /
    • 2010
  • This paper analyzes potential interference effect of DTV service on Radio Microphone in adjacent channel. For analysis, we assume that DTV operates on CH 51(692 MHz ~ 698 MHz) and radio microphone operates on CH 52 (698 MHz ~ 704 MHz) according to the Korea Communications Commission policy. Minimum Coupling Loss(MCL) method is used to determine the required protection distance and Monte Carlo method is used to determine guard band to meet interference probability of 0 % below. In conclusions, protection distance of 1.71 km and guard band of 2.4 MHz are required for indoor and protection distance of 18.86 km and guard band of 5.7 MHz are required for outdoor.

Implementation of Bayesian Filter Method and Range Measurement Analysis for Underwater Robot Localization (수중로봇 위치추정을 위한 베이시안 필터 방법의 실현과 거리 측정 특성 분석)

  • Noh, Sung Woo;Ko, Nak Yong;Kim, Tae Gyun
    • The Journal of Korea Robotics Society
    • /
    • v.9 no.1
    • /
    • pp.28-38
    • /
    • 2014
  • This paper verifies the performance of Extended Kalman Filter(EKF) and MCL(Monte Carlo Localization) approach to localization of an underwater vehicle through experiments. Especially, the experiments use acoustic range sensor whose measurement accuracy and uncertainty is not yet proved. Along with localization, the experiment also discloses the uncertainty features of the range measurement such as bias and variance. The proposed localization method rejects outlier range data and the experiment shows that outlier rejection improves localization performance. It is as expected that the proposed method doesn't yield as precise location as those methods which use high priced DVL(Doppler Velocity Log), IMU(Inertial Measurement Unit), and high accuracy range sensors. However, it is noticeable that the proposed method can achieve the accuracy which is affordable for correction of accumulated dead reckoning error, even though it uses only range data of low reliability and accuracy.

Analysis on the Impact of UWB Sensor on Broadband Wireless Communication System (UWB 센서에 의한 광대역 무선 시스템의 간섭 영향 분석)

  • Cheng, Yan-Ming;Lee, Il-Kyoo;Lee, Yong-Woo;Oh, Seung-Hyeub;Cha, Jae-Sang
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.10 no.3
    • /
    • pp.83-89
    • /
    • 2010
  • This paper presents the impacts of Ultra Wide-Band(UWB) sensor using frequency of 4.5 GHz on Broadband Wireless communication system which uses frequency of 4.5 GHz. The Minimum Coupling Loss (MCL) method and Spectrum Engineering Advanced Monte Carlo Analysis Tool (SEAMCAT) is used to evaluate the interference effects of UWB sensor on Broadband Wireless communication system, respectively. The minimum protection distance between single UWB sensor and mobile station of Broadband Wireless communication system should be more than 1.2 m to guarantee the co-existence. In case of multiple UWB sensors, UWB transmitting PSD of around -68.5 dBm/MHz below should be required to guarantee interference probability of 5% below for mobile station of Broadband Wireless communication system.

Localization Algorithm in Wireless Sensor Networks Using a Directional Antenna (지향성 안테나를 이용한 무선 센서 네트워크에서의 위치 인식 알고리즘)

  • Hong, Sung-Hwa;Kang, Bong-Jik
    • Journal of the Korea Society of Computer and Information
    • /
    • v.15 no.1
    • /
    • pp.111-118
    • /
    • 2010
  • The proposed algorithm to be explained in this paper is the localization technique using directional antenna. Here, it is assumed that anchor node has the ability to transfer the azimuth of each sector using GPS modules, sector antenna, and the digital compass. In the conventional sensor network, the majority of localization algorithms were capable of estimating the position information of the sensor node by knowing at least 3 position values of anchor nodes. However, this paper has proposed localization algorithm that estimates the position of nodes to continuously move with sensor nodes and traveling nodes. The proposed localization mechanisms have been simulated in the Matlab. The simulation results show that our scheme performed better than other mechanisms (e.g. MCL, DV-distance).

Study on Radioactive Material Management Plan and Environmental Analysis of Water (I) Study of Radioactive Substances in Water Management and Analysis to Eat of the US Environmental Protection Agency (Envirionmental Protection Agency) (물 환경의 방사성 물질 관리 방안과 분석법에 관한 연구 (I) 미국환경보호청(Envirionmental Protection Agency)의 먹는 물 방사성물질 관리와 분석법에 관한 고찰)

  • Her, Jae;Kim, Jung-Min;Min, Hye-Lim;Han, Seong-Gyu;Lim, Hyun-Jong;Jo, Han-Byeol;Noh, Young-Hoon;Lee, Ho-Sun;Park, Min-Suk
    • Journal of radiological science and technology
    • /
    • v.38 no.2
    • /
    • pp.163-170
    • /
    • 2015
  • The interest of the people in the radioactive contamination of the water has increased significantly and the study about analysis and management of radioactive materials are being actively conducted. And monitoring spots have been expanded to the range of public water as well as drinking water by publishing the rule of drinking water quality standards and examination in the Environmental Enforcement Ordinance No. 553 of Korea. In this study, US EPA was investigated as the foreign advanced cases and the way that is appropriate for the Korea was sought by analyzing investigate radionuclide, interval and management. As a result, in the selection part of investigate radionuclide, geological survey, status of nuclear power plants and the presence of the use of artificial radionuclides of the Korea should be investigated and additionally after the selection of a few radionuclides, the systems should be extended to cover all possible radionuclides by considering radioactive pollution levels in humans may be exposed due to the annual drinking water. In the part of the investigate interval, the concept(MCL, DL) should be set up for preventing concentration detection of above MCL and it needs to the maintenance and management. For example, when the concentration is more than MCL, it should be investigated on a quarterly and when the concentration is lower than MCL, it should be investigated to each different interval and management. And the US EPA divided the management area and make the roadmap for managing drinking water. The each classified area has been organized to match the state budget and labor force and the individual data have been managed effectively by HPGe, the NaI, TLD and so on.

Compatibility between ISM Equipment and WiBro Service at 2.3 GHz band (2.3 GHz 대역의 ISM 기기와 WiBro 서비스의 양립성 분석)

  • Shim, Yong-Sup;Lee, Il-Kyoo
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.11 no.2
    • /
    • pp.101-105
    • /
    • 2011
  • This paper calculates protection distance to protect WiBro service from ISM(Industrial Scientific Medical) equipment which is used in the same frequency with WiBro. We analyze with a scenario that WiBro receiver is closed to ISM equipment which has interfering power of CISPR(International Special Committee on Radio Interference) 11 limit. As a result, the protection distance of 14 m was calculated by using MCL (Minimum Coupling Loss) for the worst case. And, the protection distance of 10 m was calculated to meet 5 % interference probability below by using MC(Monte Carlo) for statistic analysis in case of a interferer.

Interference Assessment between Radio-Navigation Satellite Service and Mobile Communication Service in Adjacent L-Band (L-대역 무선항행위성업무와 이동업무간 인접대역 간섭 평가)

  • Jeong, Namho;Oh, Dae-Sub;Ku, Bon-Jun
    • Journal of Satellite, Information and Communications
    • /
    • v.8 no.4
    • /
    • pp.58-63
    • /
    • 2013
  • Since radio interference can degrade the performance of systems or limit the system operation, an accurate assessment of radio interference with the existing systems should be conducted prior to the operation of a new system. In this paper, we present an evaluation methodology for the radio interference between radio-navigation satellite service (RNSS) systems and mobile communication service (MS) system. Radio interferences from RNSS systems into MS system using minimum coupling loss (MCL) method are simulated and vice-versa, and the frequency sharing condition between two systems are derived in a same geographical area.