• Korean Journal of Food Science and Technology
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• v.51 no.3
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• pp.272-277
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• 2019

The effects of medium-chain enriched diacylglycerol (MCE-DAG) oil on hepatic cholesterol homeostasis were investigated. HepG2 hepatocytes were treated with either 0.5, 1.0, or $1.5{\mu}g/mL$ of MCE-DAG for 48 h. There was no evidence of cytotoxicity by MCE-DAG up to $1.5{\mu}g/mL$. The level of proteins for cholesterol uptake including CLATHRIN and LDL receptor increased by MCE-DAG in a dose-dependent manner (p<0.05). Furthermore, proprotein convertase subtilisin/kexin type 9, an inhibitor of LDLR, was dose-dependently diminished (p<0.05), indicating cholesterol clearance raised. MCE-DAG significantly increased 3-hydroxy-3-methylglutaryl-coenzyme A reductase and acetyl-CoA acetyltransferase2 (p<0.05), required for cholesterol synthesis, and their transcriptional regulator sterol regulatory element-binding protein2 (p<0.05). These findings suggest that given conditions of prolonged sterol fasting in the current study activated both hepatic cholesterol synthesis and clearance by MCE-DAG. However, total intracellular level of cholesterol was not altered by MCE-DAG. Taken together, MCE-DAG has the potential to prevent hypercholesterolemia by increasing hepatic cholesterol uptake without affecting intracellular cholesterol level.

• Nutrition Research and Practice
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• v.9 no.5
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• pp.489-495
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• 2015

BACKGROUND/OBJECTIVES: The present study was conducted to investigate the effects of dried Momordica charantia aqueous extracts (MCA) and ethanol extracts (MCE) on obesity and lipid profiles in mice fed a high-fat diet. MATERIALS/METHODS: Forty two ICR mice were randomly divided into six groups. The normal group was fed a basal diet, and other groups were fed a 45% high-fat diet (HFD) for 7 weeks. The normal and HFD groups were also orally administered distilled water each day for 7 weeks. The remaining groups received Momordica charantia extract (0.5 or 1.0 g/kg/day MCA, and 0.5 or 1.0 g/kg/day MCE). In order to measure the anti-obesity and lipid profile improvement effects, body and visceral tissue weight, lipid profiles, plasma insulin levels, hepatic malondialdehyde (MDA) levels and superoxide dismutase (SOD) activity were measured. RESULTS: Both MCA and MCE significantly decreased body and visceral tissue weight relative to those of the HFD group (P < 0.05). Additionally high doses of MCE and MCA significantly reduced the plasmatic insulin levels compared to the HFD groups (P < 0.05) to concentrations comparable to those found in the normal group. MCA and MCE supplementation also significantly modulated the lipid profiles in plasma, liver, and feces compared to mice fed the HFD (P < 0.05). Furthermore MCA and MCE significantly increased hepatic SOD activity, and reduced MDA generation in the liver of the HFD mice (P < 0.05). CONCLUSIONS: Results from the present study suggest that Momordica charantia extracts have anti-obesity effects and the ability to modulate lipid prolife of mice fed a HFD by suppressing body weight gain, visceral tissue weight, plasma and hepatic lipid concentrations, and lipid peroxidation along with increasing lipid metabolism.

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.5
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• pp.74-85
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• 2022

The purpose of this paper is to review the applicability of the current standards for the evaluation method of input variables and performance level in seismic performance evaluation by dynamic plastic analysis of the concrete gravity-type dam to which MCE is applied, and to suggest improvements. To this end, a domestic concrete gravity-type dam was selected as a target facility, dynamic plasticity analysis was performed under various conditions, and applicability to input variables such as concrete tensile strength and breaking energy, was reviewed. By analyzing the effect of cracks at the bottom of the gravity dam on the stability of the activity, an improvement plan for the performance level evaluation method required to secure the water storage function was derived. If the proposed improvement plan is applied, it will have the effect of deriving more reasonable evaluation results than the current seismic performance evaluation method to which MCE is applied.

• Journal of Nutrition and Health
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• v.50 no.5
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• pp.415-425
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• 2017

Purpose: Many studies have suggested that neuronal cells protect against oxidative stress-induced apoptotic cell death by polyphenolic compounds. We investigated the neuroprotective effects and the mechanism of action of Momordica charantia ethanol extract (MCE) against $H_2O_2-induced$ cell death of human neuroblastoma SK-N-MC cells. Methods: The antioxidant activity of MCE was measured by the quantity of total phenolic acid compounds (TPC), quantity of total flavonoid compounds (TFC), and 2,2-Diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging activity. Cytotoxicity and cell viability were determined by CCK-8 assay. The formation of reactive oxygen species (ROS) was measured using 2,7-dichlorofluorescein diacetate (DCF-DA) assay. Antioxidant enzyme (SOD-1,2 and GPx-1) expression was determined by real-time PCR. Mitogen-activated protein kinases (MAPK) pathway and apoptosis signal expression was measured by Western blotting. Results: The TPC and TFC quantities of MCE were 28.51 mg gallic acid equivalents/extract g and 3.95 mg catechin equivalents/extract g, respectively. The $IC_{50}$ value for DPPH radical scavenging activity was $506.95{\mu}g/ml$ for MCE. Pre-treatment with MCE showed protective effects against $H_2O_2-induced$ cell death and inhibited ROS generation by oxidative stress. SOD-1,2 and GPx-1 mRNA expression was recovered by pre-treatment with MCE compared with the presence of $H_2O_2$. Pre-treatment with MCE inhibited phosphorylation of p38 and the JNK pathway and down-regulated cleaved caspase-3 and cleaved PARP by $H_2O_2$. Conclusion: The neuroprotective effects of MCE in terms of recovery of antioxidant enzyme gene expression, down-regulation of MAPK pathways, and inhibition apoptosis is associated with reduced oxidative stress in SK-N-MC cells.

• Journal of Life Science
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• v.25 no.6
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• pp.638-645
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• 2015

This study analyzed the physiological quality of Mesembryanthemum crystallinum (ice plant) extract. M. crystallinum is a succulent plant found in Africa, southern Europe, North America, South America, and Australia. It has known antidiabetic, antioxidant, and activation of lipid metabolism effects. Extracts from M. crystallinum were prepared with methanol (MCM), ethanol (MCE), hot water (MCHW), and methanol after hot water (MCHM) extractions. The yields of MCM, MCE, MCHW, and MCHM were 0.37, 0.33, 0.50, and 0.07%, respectively. To determine the biological activities of the extracts, mushroom tyrosinase, pancreatic lipase, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging, nitric oxide (NO) production, and α-glucosidase assays were conducted. The DPPH radical scavenging activity of the MCHW extract was 62.9% at a concentration of 400 μg/ml, which was the highest of all the extracts. The MCM extract showed the highest inhibition activity of α-glucosidase and NO production (56.6 and 57.2%, respectively). The pancreatic lipase inhibition of the MCE extract was similar to that of the MCM extract, with significant inhibition of 90%. The mushroom tyrosinase inhibition of all the extracts was very low (approximately 30%). These results suggest that extracts from M. crystallinum have antioxidant, anti-inflammatory, antiobesity, and antidiabetic activities. Thus, it may have potential as a functional food product and therapeutic potential as an antidiabetic or antiobesity agent.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.368-368
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• 2020

최근 초대형화 되어 나타나고 있는 이상홍수와 지진 등에 의한 저수지 붕괴와 같은 대규모 비상상황 발생으로 하류지역 주민의 생명과 재산의 피해가 발생하고 있다. 국내의 경우 1996년 이후로 지속적으로 발생하고 있는 이상홍수로 인해 1998년에는 40개,1999년에는 5개의 소규모 저수지가 붕괴되었으며 최근 2013년과 2014년에도 저수지가 붕괴되는 상황이 발생했다. 댐붕괴의 원인은 구조물의 자연적 노화, 극심한 강우나 홍수, 지진, 제체전도, 파이핑, 침윤발생, 월류 및 파랑 등에 의한 자연적 상황 등이 요인이 될 수 있으며, 시공결함, 사고 또는 전쟁과 같은 인위적인 요인으로 발생할 수도 있다. 과거에 설계 및 시공기술이 부족하였거나 경제적인 이유로 부실하게 건설되어 있는 댐이 세계적으로 산재되어 있어 잠재적인 위험을 상당수 내재하고 있는 실정이다. 본연구는 댐의 점진적인 파괴에 의해 발생하는 유출수문곡선을 구하고 파괴의 성질을 예측 및 홍수파를 수리학적으로 추적하기위해 BREACH 모형과 DAMBRK 모형을 사용했으며 극한홍수(PMF)조건과와 최대지진발생(MCE)조건을 적용하여 원주시 관내 저수지 붕괴 모의 시나리오를 구축했다. 저수지 붕괴에 따른 유출수문곡선을 유도하기 위해서 본 연구에서는 기존의 EAP보고서 자료를 참고하여 붕괴지속시간, 붕괴부 평균폭, 붕괴부 측벽면 경사의 변화에 따라 다양한 모의를 수행함으로써 발생되는 붕괴부 유량 수문곡선을 도출하여 각각의 조건들이 붕괴파 형성에 미치는 영향에 대한 분석을 실시하였다. 그 결과 저수지의 붕괴시 첨두유출량에 민감한 영향을 주는 인자는 붕괴지속시간과, 붕괴부 평균폭으로서 이들 값이 붕괴유출량 변화에 많은 영향을 주는 것으로 나타났다. 최대지진발생(MCE)조건 해석결과 홍수류의 범람으로 인해 홍수파가 하류측으로 진행할수록 완만히 감소하며, 하천 중·상류부 인근 제내지로 홍수류의 범람이 발생하는 것으로 검토되었으며, 극한홍수(PMF)조건 해석결과 최대지진발생(MCE)조건과 같이 홍수파가 하류측으로 진행할수록 완만히 감소하는 특성을 보이며, 하천 전체 구간에서 인근제내지로 홍수류의 범람이 발생하는 것으로 검토되었다. 본 연구는 침수구역 피해규모 산정 및 비상대처계획도를 작성시 기초데이터가 되어 상황별 피해예상지역에 대해 응급행동요령, 주민대피계획비상대처계획을 수립하여 지역 주민생활에 안정을 기여하고자 한다.

• Journal of The Korean Society of Agricultural Engineers
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• v.49 no.6
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• pp.105-114
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• 2007

This study is to predict the spatial expansion of urban areas by applying CA(Cellular Automata)-Markov technique considering MCE(multi-criteria evaluation) and MOLA(multi-objective land allocation) of factor analysis. For the 10 administration districts$(3677.3km^2)$ including the whole Anseong-cheon watershed, the past six temporal land use data(1973, 1981, 1985, 1990, 1994, 2000) from Landsat satellite images were prepared. During this period, the urban area increased $233.71km^2$. Using the 36 indices composed of topological characteristics, population and land use change, the final factor map of MOLA was produced through 5 maps of MCE. Using 1990 and 1994 land use data, the 2000 predicted urban area of CA-Markov with factor map showed 0.06% improvement of absolute error comparing with that of CA-Markov without factor map. By the CA-Markov technique considering factor map, the 2030 and 2060 urban area increased $58.94km^2(0.78%)\;and\;60.14km^2(0.81%)$ respectively comparing with 2000 urban area$(313.19km^2)$. The 2030 and 2060 paddy area decreased $93.28km^2(2.54%)\;and\;93.65km^2(2.55%)$ respectively comparing with 2000 paddy area$(1383.23km^2)$.

• Food Science of Animal Resources
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• v.42 no.4
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• pp.609-624
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• 2022

Tenebrio molitor larvae, as known as edible insects, has advantages of being rich in protein, and has been recognized as a suitable alternate protein source for broiler and pig feed. Moreover, given their ability to biodegrade polystyrene, a major pollutant, Tenebrio molitor larvae has been proposed as an innovative solution to environmental problems. In the present study, we investigated the toxicity of Tenebrio molitor larvae powder (TMlp) ingested with expanded-polystyrene (W/ eps) through in vitro and in vivo experiments. The objective of this study was to determine whether TMlp W/ eps can be applied as livestock alternative protein source. For in vitro experiments, cytotoxicity test was performed to investigate the effects of TMlp-extract on the viability of estrogen-dependent MCF-7 cells. The possibility of estrogen response was investigated in two groups: Expanded-polystyrene-fed (W/ eps) TMlp group and without expanded-polystyrene-fed (W/o eps) TMlp group. For in vivo experiments, The male Sprague-Dawley rats were divided based on the dosage of TMlp administered and oral administration was performed to every day for 5 weeks. A toxicological assessments were performed, which included clinical signs, food consumption, body and organ weights, hematology, serum chemistry, and hematoxylin and eosin staining of liver and kidney. There were no specific adverse effect of TMlp W/ eps-related findings under the experimental conditions of this study, but further studies on both sexes and animal species differences should be investigated. In conclusion, TMlp W/ eps was considered non-toxic and observed to be applicable as an alternative protein source for livestock feed.

• The Journal of the Acoustical Society of Korea
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• v.27 no.5
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• pp.252-255
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• 2008

In this paper, we apply a discriminative weight training to a support vector machine (SVM) based gender identification. In our approach, the gender decision rule is expressed as the SVM of optimally weighted mel-frequency cepstral coefficients (MFCC) based on a minimum classification error (MCE) method which is different from the previous works in that different weights are assigned to each MFCC filter bank which is considered more realistic. According to the experimental results, the proposed approach is found to be effective for gender identification using SVM.

• Earthquakes and Structures
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• v.15 no.1
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• pp.45-65
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• 2018

Over the last three decades, Performance-based Earthquake Engineering (PBEE) has been mainly developed for high seismicity regions. Although information is abundant for PBEE throughout the world, the application of PBEE to lower-seismicity regions, such as those where the magnitude of the maximum considered earthquake (MCE) is less than 6.5, is not always straightforward because some portions of PBEE may not be appropriate for such regions due to geological differences between high- and low-seismicity regions. This paper presents a brief review of state-of-art PBEE methodologies and introduces the seismic hazard of lower-seismicity regions, including those of the Korean Peninsula, with their unique characteristics. With this seismic hazard, representative low-rise RC MRF structures and high-rise RC wall residential structures are evaluated using PBEE. Also, the range of the forces and deformations of the representative building structures under the design earthquake (DE) and the MCE of South Korea are presented. These reviews are used to propose some ideas to improve the practice of state-of-art PBEE in lower-seismicity regions.