• 대한수의학회지
• /
• 제31권2호
• /
• pp.179-187
• /
• 1991

These studies were carried out to investigate the effects of the size of follicles, semen types, capacitation methods, and additions of hormones, estrous cow serum(ECS), fetal calf serum(FCS), bovine follicular fluid(BFF) and matured cumulus cell(MCC) to the medium on in vitro maturation and fertilization of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluid from the visible follicles of diameter 3~5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones, FCS, ECS, BFF and MCC for 24~48hrs. in an incubator with 5% $CO_2$ in air at $38.5^{\circ}C$ and then matured oocytes were again cultured for 18~20 hrs. with motile capacitated spermatozoas the TCF (Tyrode calcium free) solution containing $100{\mu}g/ml$ of heparin. The results obtained in these experiments were summarized as follows: 1. The oocytes classified as "A,B,C,D and Degenerative" depending morphological integrity and those 61.4%, 12.1%, 19.2%, 4.2% and 3.0% of the total oocytes recovered, respectively. The maturation and fertilization rate of the A, B, C class follicular oocytes, cultured in the TCM-199 medium supplemented with 10% FCS were 89.1%, 78.0%, 52.6% and 78.1%, 66.1, 33.3%, respectively. 2. The average number of the follicular oocytes recovered from follicles size, 1~2mm, 3~5mm and above 5mm in diameter were 67, 98 and 63, respectively. The maturation and fertilization rate of the follicular oocytes, cultured in the TCM-199 medium were 56.7%, 82.5%, 46.0% and 44.8%, 71.4%, 28.6%, respectively. 3. The fertilization and cleavage rate of the follicular oocytes, inseminated with spermatozoas of epididymal cauda, neat and frozen semen were 63.3%, 73.3%, 70.0% and 32.7%, 37.8% 38.3%, respectively. 4. The fertilization and cleavage rate of follicular oocytes, fertilized with capacitated spermatozoas by heparin, BFF and HIS methods were 70.0%, 53.8%, 34.2% and 38.3%, 23.1%. 17.1%, respectively. And the fertilization and cleavage rate were higher method of heparin. 5. The maturation and fertilization rate of follicular oocytes, cultured in the TCM-199 medium supplemented with 5%, 10%, 15%, 20% and FSH, HCG, 17, $\beta$-estradiol were 76.0~82.3% and 26.2~70.0%, and those values were higher the supplementation than non-supplementation. 6. The maturation and fertilization rate of the follicular oocytes cultured in TCM-199 medium supplemented with 5~20% ECS and FCS were 74.0~80.6%, 26.2~30.0% and 71.7~76.9%, 51.9~58.0%, and the values were higher the supplement of ECS than FCS. 7. The maturation rate (68.0~64.6%) and fertilization rate(59.6~60.4%) of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and 20~30% BFF were higher than those of follicular oocytes cultured TCM-199 medium supplemented with 10% FCS and 10% and 50% BFF. 8. The maturation rate(76.5%) and fertilization rate (61.7%) of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and $1{\times}10^6/ml$ MCC were higher than those of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and $1{\times}10^{{4}{\sim}{5}}/ml$ and $1{\times}10^8/ml$ cumulus cells.

• 한국전산구조공학회논문집
• /
• 제24권3호
• /
• pp.295-305
• /
• 2011

본 연구에서는 원자력발전소의 주요 설비중의 하나인 전기설비를 대상으로 지진취약도 재평가를 위한 진동대 실험을 수행하였다. 원자력발전소 내에는 많은 전기설비들이 설치되어 있으며, 이러한 전기설비의 손상은 전기설비 자체의 손상에서 그치는 것이 아니고 발전소 전체의 안전성에 큰 영향을 미칠 수 있다. 따라서 원자력발전소의 확률론적 지진안전성 평가에서는 주요 전기설비에 대한 지진취약도 결과를 활용한 평가를 수행하고 있다. 본 연구에서는 기존의 확률론적 지진안전성 평가에서 사용하고 있는 전기설비의 지진취약도 값에 대한 재평가를 위하여 원자력발전소에서 사용하고 있는 주요 기기에 대한 진동대 실험을 수행하였다. 평가대상 전기설비로는 480V MCC를 선정하였으며, 진동대 실험을 위하여 NRC 설계지진, 등재해도 스펙트럼에 의한 인공지진 그리고 PAB165'에서의 층응답스펙트럼을 이용한 인공지진의 3가지 지진파를 이용하였다. 설계지진동 수준인 최대지반가속도 0.2g부터 단계적으로 입력수준을 증가시키면서 실험을 수행하였다. NUREG/CR-5203에서 제시하고 있는 방법에 의거하여 캐비넷에서의 증폭비를 비교하였으며, EPRI TR-103959의 방법으로 취약도 평가를 수행하여 기존의 확률론적 지진안전성 평가에서 사용하고 있는 지진취약도 결과와 비교하였다. 결론적으로 기존의 보고서에서 제시하고 있는 취약도 결과가 다소 보수적으로 평가하고 있음을 알 수 있었다.

• 한국가축번식학회지
• /
• 제14권3호
• /
• pp.183-190
• /
• 1990

These studies were carried out ot investigate the effects of estrous cow serum(ECS), fetal calf serum(FCS), bovine follicular fluid(BFF) and matured cumulus cell(MCC) on in vitro maturation and fertilization of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluid from the visible follicles of diameter 3-5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones, FCS, ECS, BFF and MCC for 24~48 hrs. in a incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 18$^{\circ}C$20 hrs. with motile capacitated sperm in the TCF(Tyrode calcium-free) solution containing 200$\mu\textrm{g}$/ml of heparin. The results obtained in these experiments were summarized as follows : 1. The oocytes classified as "A, B, C, D and Degenerative" depending morphological integrity and those were 61.4%, 12.1%, 19.2%, 4.2% and 3.0% of the total oocytes harvested, respectively. The maturation and fertilization rate of the A, B, C class follicular oocytes, cultured in the TCM-199 medium supplemented with 10% FCS were 89.1%, 78.0%, 52.6% and 78.1%, 33.3%, respectively. 2. The maturation and fertilization rate of the follicular oocytes cultured in TCM-199 medium supplemented with 5%~20% ECS and FCS were 74.0%~80.6, 26.2%~30.0% and 71.7%~76.9%, 51.9%~58.0%, and those values were higher the supplement of ECS than FCS. 3. The maturation rate(68.0%~64.6%) and fertilization rate(59.6%~60.4%) of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and 20~30% BFF were higher than those of follicular oocytes cultured TCM-199 medium supplemented with 10% FCS and 10% and 50% BFF. 4. The maturation rate(76.5%) and fertilization rate(61.7%) of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and 1$\times$106/ml cumulus cells were higher than those of follicular oocytes cultured in TCM-199 medium supplemented with 10% FCS and 1$\times$104~5/ml and 1$\times$108/ml cumulus cells.lus cells.

• 대한교통학회지
• /
• 제31권2호
• /
• pp.45-59
• /
• 2013

이 연구는 우리나라에서 현재까지 사용되어 온 (수단O/D기반)화물수요추정법의 문제점 인식을 바탕으로 P/C기반 화물수요추정법과 비교 분석을 수행하였다. 우리나라 화물수단O/D는 화물의 최초기점과 최종종점 간 운송을 수단별 통행으로 나누어 인식하면서 접근트럭통행(Access/Egress Truck Trip)에 대한 정보가 누락되어있다. 이러한 이유로 수단O/D기반 화물수요추정법은 화물의 복합수단운송을 반영하지 못하는 단점이 있다. 이에, 본 연구는 우리나라 컨테이너 화물을 중심으로 P/C표와 복합수단선택모형을 추정하여 P/C기반 화물수요추정법을 제시하고, 기존 사용되어 오던 수단O/D기반 화물수요추정법과 기종점쌍 간 전환량, 수단통행물동량, 링크물동량을 비교하였다. 비교 결과 P/C기반 화물수요추정법은 복합운송을 제대로 모사하지 못하는 기존 수단O/D기반 화물수요추정법의 문제점을 해결할 수 있는 것으로 평가되었다.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2011년도 제41회 하계 정기 학술대회 초록집
• /
• pp.310-311
• /
• 2011

Dual-frequency (DF) capacitively coupled plasmas (CCP) are used to separately control the mean ion energy and flux at the electrodes [1]. This separate control in capacitively coupled radio frequency discharges is one of the most important issues for various applications of plasma processing. For instance, in the Plasma Enhanced Chemical Vapor Deposition processes such as used for solar cell manufacturing, this separate control is most relevant. It principally allows to increase the ion flux for high deposition rates, while the mean ion energy is kept constant at low values to prevent highly energetic ion bombardment of the substrate to avoid unwanted damage of the surface structure. DF CCP can be analyzed in a fashion similar to single-frequency (SF) driven with effective parameters [2]. It means that DF CCP can be converted into SF CCP with effective parameters such as effective frequency and effective current density. In this study, comparison of DF CCP and its converted effective SF CCP is carried out through particle-in-cell/Monte Carlo (PIC-MCC) simulations. The PIC-MCC simulation shows that DF CCP and its converted effective SF CCP have almost the same plasma characteristics. In DF CCP, the negative resistance arises from the competition of the effective current and the effective frequency [2]. As the high-frequency current increases, the square of the effective frequency increases more than the effective current does. As a result, the effective voltage decreases with the effective current and it leads to an increase of the ion flux and a decrease of the mean ion energy. Because of that, the negative resistance regime can be called the preferable regime for solar cell manufacturing. In this preferable regime, comparison of DF (13.56+100 or 200 MHz) CCP and SF (60 MHz) CCP with the same effective current density is carried out. At the lower effective current density (or at the lower plasma density), the mean ion energy of SF CCP is lower than that of DF CCP. At the higher effective current density (or at the higher plasma density), however, the mean ion energy is lower than that of SF CCP. In this case, using DF CCP is better than SF CCP for solar cell manufacturing processes.

• 한국정보통신학회논문지
• /
• 제21권2호
• /
• pp.379-385
• /
• 2017

일반적으로 배전반, 분전반, 전동기제어반(Motor Control Center; MCC)은 집단거주지역, 빌딩, 학교, 공장, 항만, 공항, 상하수 처리장, 변전소, 중공업 플랜트 등의 광범위한 전력 수용가에 설치되어 특고압의 전력을 해당 설비들에 요구되는 전압으로 변환하여 공급하는데 사용된다. 이와 같은 배전반, 분전반, MCC에 포함되는 전기설비의 사고는 부스바 접촉부의 열화에 의한 사고, 부스바의 접점 및 연결 부위에서의 접촉 불량에 의한 사고, 부스바 접촉부의 과열현상에 의한 사고로 구분된다. 본 논문에서는 부스바 접촉부의 볼트 및 너트의 풀림상태, 접촉부 열화 측정이 가능하며, 정밀 가변저항을 이용하여 저항값의 변화에 따라 볼트 체결상태 감지 및 비접촉식 적외선 센서를 사용하여 부스바 접촉부 온도 감지 시스템을 설계하고 구현하였다. 부스바 접촉부 체결상태 감지를 위한 가변저항을 이용한 실험을 수행한 결과 볼트와 너트를 완전히 체결시키면 가변저항 값은 감소하였으며, 최대 오차범위는 0.1mm의 결과를 보였다. 또한, 부스바 접촉 저항값 변화에 따라 접촉부 온도가 $27.3^{\circ}C$에서 $69.3^{\circ}C$로 상승하는 결과를 확인하였다.

• 한국시뮬레이션학회논문지
• /
• 제31권3호
• /
• pp.45-54
• /
• 2022

본 논문은 화생방 정찰차-II(장갑형)용 모의훈련장비(시뮬레이터)를 설계하고 구현한 논문이다. 모의훈련장비(시뮬레이터)는 군에서 화생방 상황에 따라 다양한 화생방 장비를 이용하여 상황을 파악하고, 상급부대로 전문작성 보고를 할 수 있는 훈련장비로 가상의 환경에서 훈련을 할 수 있는 장비 이다. 통제관 또는 훈련교관은 발생할 수 있는 화생방 상황을 교관통제 시스템을 이용하여 시나리오를 구성하고, 상황을 전파 할 수 있으며, 전파된 상황을 훈련생이 적절히 수행 하는 과정을 관찰할 수 있다. 모든 과정은 시스템에 의해서 모니터링 및 분석이 가능하고, 녹화가 가능하여 사후검토에도 사용된다. 가상의 환경에서 화생방 상황 훈련을 구현하기 위해 교관통제(IOS), 호스트(HOS), 영상(IGS), 입출력장치(IOC), 음향(ACS)를 구현하였으며, 화생방 탐지 장비인 원거리 화학 자동 경보기(LCA), 화생겸용 자동 탐지기(CAD), 통제(MCC) 및 운용(OCC) 컴퓨터를 시뮬레이터로 개발 하였다. 본 논문에서는 화생방 정창차-II(장갑형)용 모의훈련장비 개발을 위한 설계와 개발을 하였으며, 통합시험 및 수락검사를 통해 성능을 검증 하였다.

• 한국환경과학회지
• /
• 제23권3호
• /
• pp.379-386
• /
• 2014

Adsorption and biodegradation performance of tetracycline antibiotic compounds such as ttetracycline (TC), oxytetracycline (OTC), minocycline (MNC), chlortetracycline (CTC), doxycycline (DXC), meclocycline (MCC), demeclocycline (DMC) on granular activated carbon (GAC) and anthracite-biofilter were evaluated in this study. Removal efficiency of seven tetracycline antibiotic compounds showed 54%~97% by GAC adsorption process (EBCT: 5~30 min). The orders of removal efficiency by GAC adsorption were tetracycline, demeclocycline, oxytetracycline, chlortetracycline, doxytetracycline, meclocycline and minocycline. Removal efficiencies of seven tetracycline antibiotic compounds showed 1%~61% by anthracite biofiltration process (EBCT: 5~30 min). The highest biodegradable tetracycline antibiotic compound was minocycline, and the worst biodegradable tetracycline antibiotic compounds were oxytetracycline and demeclocycline.

• 오토저널
• /
• 제14권6호
• /
• pp.67-73
• /
• 1992

This paper describes Hyundai's research and development work on a flexible fuel vehicle (FFV). The work on FFV has been conducted to evaluate its potential as an alternative to the conventional gasoline vehicle. Hyundai FFV described here can be operated on M85, gasoline, or any of their combinations, in which the methanol concentration is measured by an electrostatic type fuel sensor. For that operation, a special FFV ECU(Eletronic Control Unit) has been developed and incorporated in the FFV. The characteristics affecting FFV operation, such as FFV ECU control strategy and injector flow rate, have been investigated and optimized through the experiment. And various development tests have been performed in view of engine performance, durability, cold startability, and exhaust emissions reduction. The exhaust gas aftertreatment system consisting of manifold type catalyst and secondary air injection system shows good emission reduction performance including formaldehyde, and finally, the possibility of the FFVs as the low emission vehicles is evaluated by presenting NMOG(Non-Methane Organic Gases) levels with respect to M0 and M85. With these results, it is concluded that FFV can be a candidate for the low emission vehicles, but more works on its durability improvement is required.

• 펄프종이기술
• /
• 제37권3호
• /
• pp.1-8
• /
• 2005

Increase in the utilization rate of recycled paper and closing level of papermaking system increased the problem associated with stickies that include decrease in process runnability and product quality. It is required to establish a process for removing the micro stickies to solve the problems associated with stickies. In this study, the application of flotation process as a method to remove micro stickies was examined. Model micro stickies (MMS) were prepared using microcrystalline cellulose (MCC) and pressure sensitive adhesives (PSA), and the influence of three nonionic surfactants on the removal efficiency of MMS from flotation process was examined. Also the effect of surfactants on the deposition of micro stickies that remaining in the papermaking wet end onto wire was examined. Removal efficiency of MMS by flotation was increased when the proportion of nonionic surfactant with propylene oxide (PO) type hydrophilic tail was increased and stock pH was 7. It was suggested that this nonionic surfactant minimized the increase of surface energy of hydrophobic MMS. The MMS with high hydrophobicity remaining in the papermaking system, however, would cause more serious deposition problems on papermaking wet end. Therefore, it is of great importance to increase the removal efficiency of MMS in flotation process for the prevention of papermaking system contamination caused by stickies deposition.