• Title/Summary/Keyword: MAT1A

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Identification of 19 Species of Poisonous Plants from Jeju Island and Construction of a Database Using DNA-barcoding (DNA-barcoding을 이용한 제주도 자생 독성 식물 19종의 종 식별 및 데이터베이스 구축)

  • Kwon, Eunchae;Kim, Joo-Young;Chang, Miwha;Lee, Minji;Moon, Seohyun;Lee, Won-Hae
    • Korean Journal of Plant Resources
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    • v.35 no.2
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    • pp.346-361
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    • 2022
  • Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.

A Study on the Evacuation Behaviors of Children with Disabilities in Child Care Center through Evacuation Experiments (장애아전문어린이집 피난훈련을 통한 피난행태 분석 연구)

  • Lee, Jeong-Soo;Oh, Young-sook;Kwon, Yong-Won
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.4
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    • pp.1-8
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    • 2020
  • This paper proposes architectural and evacuation recommendations through evacuation experiments of two child care centers with disabilities. To achieve these purposes, a survey on the architectural characteristics and evacuation experiments was carried out. The results were as follows: (1) the child care center with disabilities were located within one kilometer or five minutes from a fire station and 119 safety center, but the architectural characteristics and the ratio of self-moving children in a care center were very different conditions. (2) The evacuation behavior patterns of children with a disability were as follows: hugging a child by a teacher with the arms, walking with hugging and holding the child by a teacher, moving a wheelchair with a helper, and drag-out a cerebral palsied child with a mat wrapping. (3) The speed of the evacuation was fast in the following order: drag-out with a mat wrapping a child with a disability, hugging the child with the arms, walking with hugging and holding child, and moving with a wheelchair. In the case of difficulties in the vertical evacuation, a temporal evacuation area was essential for children with disability.

A Study on the Mix Design and the Control System of Thermal Crack for High Quality Mass Concrete (고품질 매스콘크리트 시공을 위한 배합설계 및 온도균열제어 시스템에 관한 연구)

  • Kim, Sun-Gu;Lee, Sang-Soo;Won, Cheol;Park, Sang-Joon;Kim, Dong-Seok
    • Journal of the Korea Institute of Building Construction
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    • v.1 no.2
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    • pp.174-178
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    • 2001
  • This study was performed to control the thermal crack of the mat footing slab in the multi-purposed buildings. In this study, we executed the mixing design of concrete to satisfy the workability and the quality according to the site conditions. And, we evaluated quantitatively about the possibility of thermal crack by using hydration heat analysis system. Finally, we proposed the optimal mixing conditions, curing methods and curing period which all factors are considered. As a result, the optimal mixing conditions were : W/B 41%, unit binder 375kgf/$\textrm{m}^3$, FA replacement ratio 20%. Lowest thermal stress was 22.0kgf/$\textrm{cm}^2$ and at that time thermal crack index was over 1.5, when the coefficient of thermal conductivity was lowest among the curing conditions. And, the total curing time was estimated at 6.7 days according to curing steps.

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Studies on Restoraation of Forest-Floor Vegetation Devastated by Recreational Trampling(II) -A Comparison of Growth for Selection of Native Tree Species- (답압으로 훼손된 임간나지의 임상식생 복원에 관한 연구(II) -자생 수종선발을 위한 생장력 비교-)

  • 오구균;우보명
    • Journal of the Korean Institute of Landscape Architecture
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    • v.21 no.1
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    • pp.51-61
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    • 1993
  • Seeding treatment was used for 2 years at an artificial bare ground for selecting tree species suitable for forest-floor revegetation. 2 ${\times}$2${\times}$2 factorial experiment was used with a randomized complete block design for 19 native tree species and results were summarized as follows; 1. Native species suitable for restoration of bareland under 75% shading in central part of Korea were Styrax japonica, Styrax obassia, Smilax china, Callicarpa japonica, Stephanandra incisa, Viburnum dilatatum for. pilosulm, Magnolia sieboldii, Cornus kousa, Celastrus orbiculata, etc.. Especially, Magnolia sieboldii, Stephanandra incisa and Stryrax obassia were tolerant for forest-floor with hardened woil surface and Callicarpa japonica, Viburnum dilatatum for. pilosulum, Euonymus sieboldianus and Philadelphus schrenkii were suitable for softened soil surface with straw-mat mulching. 2. It is necessary to break seed dormancy to accelerate germination in case of Styrax obassia, Styrax japonica, Viburnum dilatatum for. pilosulum, Smilax china and Stephanandra incisa.

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Morphological and Phylogenetic Characteristics of Tuber himalayense Collected from Rhizosphere of Quercus dentata in Korea

  • Park, Hyeok;Gwon, Ju-Hui;Lee, Jong-Chul;Kim, Hyun Suk;Seo, Geon-Sik;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.1
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    • pp.101-108
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    • 2021
  • We collected the ascomata of Tuber species from the rhizosphere of Quercus dentata in Danyang, Korea. We observed the morphological characteristics of ectomycorrhizal roots and ascomata, and identified the species based on the results of the phylogenetic analysis conducted using the DNA sequences of an internal transcribed spacer, a large-subunit rDNA, translation elongation factor 1-α DNA (TEF1), and MAT. Finally, we identified the fungal species as Tuber himalayense B.C. Zhang & Minter, which has not been recorded previously in Korea. We evaluated the morphological characteristics and conducted phylogenetic analysis of the ascoma and mycorrhiza (associated with Q. dentata) of T. himalayense.

Effect of Water Absorption on Fatigue Crack Growth Behavior of E-Glass/Polyerter Composite (E-Glass/Polyester 복합재료의 피로균열성장거동에 미치는 흡습의 영향)

  • Kim, Yon-Jig;Kweon, Il-Hyun;Lim, Jae-Kyoo;Jeong, Se-Hui
    • Korean Journal of Materials Research
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    • v.3 no.1
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    • pp.84-94
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    • 1993
  • This paper deals with the fatigue properties of chopped strand glass mat/polyester composite to understand the effect of water absorption on fatigue behavior of GFRP. The fatigue crack in the both no water and a water absorption materials initiated at the initial of cycle. Thereafter, it was divided with two regions that one decreased with the crack extension and the other increased with the crack extension. The absorption of distilled water degrades the bond strength between fiber and matrix, there, by the tendency of fiber pull-out is increased in perpendicular to crack growth deirection and the debonding of fibers increased to the place which is parallel to crack growth direction. Therefore, the reduction of fatigue strength was caused by these factors.

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Case of Prenatally Diagnosed, 3 Successive Familial Partial Trisomy 4p nd 4/22 Translocation of Maternal Origin (산전 유전 검사로 진단된 3회 연속적인 모계 기원의 가족성 partial trisomy 4p와 4/22 전좌 이상(translocation) 예)

  • Yang, Y.H.;Kim, G.S.;Kim, S.K.;Kim, I.K.;Min, H.W.;Song, C.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.21 no.1
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    • pp.131-135
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    • 1994
  • A 27-year-old pregnant woman who had one son with mental and growh retardation and dysmorphic features, was referred for genetic counselling. Cytogenetic investigations revealed 4/22 translocation in the mother(46, XX, t(4;22)(p14;P11)), partial trisomy 4p in son(46, XY, -22, +der(22), t(4;22)(p14;p11)mat). The father had normal karyotype. Amniocentesis and chorionic villi sampling were performed in 3 successive pregnancies. The karyotypes of fetus in 3rd, 4th pregnancies by amniocentesis were 46, XX, t(4;22)(p14;p11) and 46, XX, t(4;22) (p14;p11), and the karyotype of fetus in 5th pregnancy by chorionic villi sampling was found to be 46, XX, -22, +der(22) t(4;22)(p14;p11)mat. We report 3 succesive prenatally diagnosed familial partial trisomy 4p and 4/22 translocation of maternal origin with review of literature.

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Comparative serology of human brucellosis in Korea

  • Lee, Sung-Il;Choi, Min-Jung;Hur, Jin;Islam, Md Ariful;Khatun, Mst Minara;Baek, Byeong-Kirl;Lee, Chang-Seup;Kakoma, Ibulaimu;Bolye, Stephen M;Srirangananathan, Nammalwar;Young, Edward John
    • Korean Journal of Veterinary Service
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    • v.31 no.3
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    • pp.331-338
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    • 2008
  • The study was carried out to evaluate the diagnostic efficacy of the tube agglutination test (TAT), enzyme-linked immunosorbent assay (ELISA) and the 2-Mercaptoethanol agglutination test (2-MAT) to detect human brucellosis patients in Korea. We examined 87 serum samples of people in the rural farm areas where bovine brucellosis had been reported. People in this study were divided into seven groups- farmers and their families, veterinarians, veterinary quarantine workers, livestock health control officers, artificial inseminators, livestock traders and healthy control individuals. Among 87 people, 65 were males and 22 were females ranging in age from 13 to 72 years. Of 87 serum samples, ELISA detected 21.84%, TAT detected 11.50% and 2-MAT detected 8.05% Brucella positive sera. Brucella specific IgG ELISA antibody titer was recorder higher in the individuals between the ages of 50 and 65 years. The highest prevalence rate of brucellosis(29.4%) was recorded in the cattle farmers and their family members followed by quarantine veterinary office workers (25%) and practicing veterinarians 01.1%). The majority of the Brucella sero-positive individuals in this study had a history of direct contact with animals.

Evaluation of NPS Pollutant Reduction of Rice Straw Mats in Field (경작지에서 볏짚거적의 비점오염물질 저감 평가)

  • Won, Chul-Hee;Shin, Min-Hwan;Choi, Yong-Hun;Lim, Kyoung-Jay;Han, Young-Han;Kwon, Jay-Hyouk;Choi, Joong-Dae
    • Journal of The Korean Society of Agricultural Engineers
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    • v.55 no.4
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    • pp.37-44
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    • 2013
  • We have examined the effect of rice straw mat (RSM) on the reduction of non-point source (NPS) pollution loads at soybean cultivations. The slope of the experimental plot was about 3 %. Monitoring was carried out for four years at conventional tillage (CT) in 2008~2009 years and RSM covered tillage in 2010~2011 years. Thirty-two rainfall events were monitored and analyzed during the study period. During the 2 years of 2008 and 2009, 20 rainfall runoff events were monitored. But in 2010 years, only 2 rainfall runoff events could be monitored. And in 2011 years, 10 rainfall runoff events was monitored. It was because the RSM cover enhanced infiltration and reduce runoff in 2010 and 2011. Average NPS pollution load (organic matters) of the RSM covered field was reduced by 72.1~94.2 % compared to that of CT field. NPS pollution load of TN and TP reduced by 67.5 % and 55.7 %, respectively. Especially, SS pollution load was reduced by 97.3 %. Based on the results, rice straw mat cover was considered as a promising best management practices (BMP) to reduce NPS pollution load. However, it was recommended that the results are limited to the field conditions and the same experiments must be performed on different soil textures, slopes, and crops if it is applied to the development of policies.

Color Change in Pressed Rose Petals as Affected by Pressing Method (압화 방법에 따른 장미 꽃잎의 변색)

  • Byun, Mi Soon;Kim, Soon Ja;Kim, Kiu Weon
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.1
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    • pp.49-56
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    • 2008
  • Typical rose flowers, 'Red Corvette (red)', 'Nobless (pink)', 'Golden Metal (yellow)', and 'Rose Yumi (white)', were used as experimental materials to examine flower color changes as affected by pressing method and light treatment for four weeks and eight weeks by 2,000 lux fluorescent light at a 16 hours daylength. Pressing materials in silicagel mat resulted in the least change after pressing and light treatment for all kinds. On the contrary, an electric pressing device caused color to change to brown. Flower colors deteriorated the most by an electric pressing device made in Korea for pink rose; an electric pressing device made in Japan for white rose; and paper sheet made in Korea for red rose. The sequence of degree in severity of color change after pressing was pink, yellow, and white roses. Light treatment brought about the most extreme color change in yellow 'Nobless' rose, while other three cultivars were rather stable in change of color.