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http://dx.doi.org/10.7732/kjpr.2022.35.2.346

Identification of 19 Species of Poisonous Plants from Jeju Island and Construction of a Database Using DNA-barcoding  

Kwon, Eunchae (Forensic DNA Division, National Forensic Service)
Kim, Joo-Young (Forensic DNA Division, National Forensic Service)
Chang, Miwha (Forensic DNA Division, National Forensic Service)
Lee, Minji (Forensic DNA Division, National Forensic Service)
Moon, Seohyun (Forensic DNA Division, National Forensic Service)
Lee, Won-Hae (Forensic DNA Division, National Forensic Service)
Publication Information
Korean Journal of Plant Resources / v.35, no.2, 2022 , pp. 346-361 More about this Journal
Abstract
Food poisoning accidents caused by poisonous plants occur every year. As certain poisonous plants are mistaken for edible plants causing food poisoning, accurate species identification of poisonous plants is required. DNA barcodes suitable for species identification of poisonous plants and database that can be used for accurate species identification are necessary for their use in forensic cases. In this study, species identification of 19 poisonous plants native to Jeju Island using seven DNA barcodes (trnH-psbA, trnL-trnF, trnL intron, rbcL, matK, ITS1-ITS4, 18S rRNA) was performed to construct a database containing sequence information and DNA barcode universality. trnL-trnF barcode and ITS1-ITS4 barcode were the easiest markers for PCR amplification and sequence retrieval, and the combination of the two barcodes enabled single species identification in 18 out of 19 plants. Therefore, when an investigation of unknown poisonous plants is requested, combination of trnL-trnF and ITS1-ITS4 barcodes is considered as a primary marker for species identification. The database of recommended DNA barcodes for each poisonous plant presented in this study will be helpful in plants poisoning cases.
Keywords
Forensic botany; Genome database; ITS1-ITS4; trnL-trnF;
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1 Ng, A.E., E. Sandoval and T.M. Murphy. 2016. Identification and individualization of Lophophora using DNA analysis of the trnL/trnF Region and rbcL Gene. J. Forensic Sci. 61:S226-S229.   DOI
2 Sang, T., D.J. Crawford and T.F. Stuessy. 1997. Chloroplast DNA phylogeny, reticulate evolution, and biogeography of Paeonia (Paeoniaceae). Am. J. Bot. 84(8):1120-1136.   DOI
3 Shaw, J., E.B. Lickey, J.T. Beck, S.B. Farmer, W. Liu, J. Miller, K.C. Siripun, C.T. Winder, E.E. Schilling and R.L. Small. 2005. The tortoise and the hare II: Relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Am. J. Bot. 92(1):142-166.   DOI
4 CBOL Plant Working Group. 2009. A DNA barcode for land plants. PNASU. 106(31):12794-12797.   DOI
5 Chun, J.B., M. Jin, N. Jeong, C. Cho, M.S. Seo, M.S. Choi, D.Y. Kim, H.B. Sohn and Y.H. Kim. 2019. Genetic identification and phylogenic analysis of new varieties and 149 Korean cultivars using 27 InDel markers selected from dense variation blocks in Soybean (Glycine max (L.) Merrill). Korean J. Plant Res. 32(5):519-542.
6 Liu, J., W. Mu, M. Shi, Q. Zhao, W. Kong, H. Xie and L. Shi. 2021. The species identification in traditional herbal patent medicine, Wuhu San, based on shotgun metabarcoding. Front. Pharmacol. 12:152.
7 Hwang, I.K., M.H. Kim, M. Kim, K.D. Kwak, E.J. Lee, Y.H. Lee and K.W. Park. 2013. A case of identification by research of microsatellite markers in pine theft. Korean Journal of Forensic Science 14(1):79-84 (in Korean).
8 Jansen, R.K., C. Kaittanis, C. Saski, S.B. Lee, J. Tomkins, A.J. Alverson and H. Daniell. 2006. Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: Effects of taxon sampling and phylogenetic methods on resolving relationships among rosids. BMC Evol. Biol. 6(1):1-14.   DOI
9 Cuenoud, P., V. Savolainen, L.W. Chatrou, M. Powell, R.J. rayer and M.W. Chase. 2002. Molecular phylogenetics of Caryophyllales based on nuclear 18S rDNA and plastid rbcL, atpB, and matK DNA sequences. Am. J. Bot. 89(1):132-144.   DOI
10 Kress, W.J., K.J. Wurdack, E.A. Zimmer, L.A. Weigt and D.H. Janzen. 2005. Use of DNA barcodes to identify flowering plants. PNASU. 102(23):8369-8374.   DOI
11 Rollo, F., M. Ubaldi, L. Ermini and I. Marota. 2002. Otzi's last meals: DNA analysis of the intestinal content of the neolithic glacier mummy from the Alps. PNASU. 99(20):12594-12599.   DOI
12 Sorhannus, U. 2004. Diatom phylogenetics inferred based on direct optimization of nuclear-encoded SSU rRNA sequences. Cladistics 20(5):487-497.   DOI
13 Sung, A.J., H. Kim, Y.J. Kang, B.H. So, M.J. Lee, K.H. Park, J.B. Park, S.R. Yeom, S.B. Oh, J.Y. You, K.W. Lee, B.J. Chun and K.W. Lee. 2012. Multicenter survey of intoxication cases in Korean emergency departments: 2nd annual report. 2009. J. Korean Soc. Clin. Toxicol. 10(1):22-32 (in Korean).
14 White, T.J., T. Bruns, S.J.W.T. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: A guide to methods and applications. Academic Press 18(1):315-322.
15 Lee, M., S. Choi, D.H. Kim, J.Y. Jung, J.Y. Kim, S. Moon and I.K. Hwang. 2020. Species identification of Lophophora williamsii (Psychoactive cactus) using botanical DNA barcodes. Journal of Science Criminal Investigation 14(1):61-67 (in Korean).   DOI
16 Bae, J.H., G.E. Kim, G.J. Kim, D.H. Lee, J.Y. Lee, D.J. Jeong, Y.S. Kim, C.W. Park, H.Y. Lee and J.H. Cho. 2013. Four cases of Phytolacca esculenta van houtte intoxication due to misidentification as a ginseng. J. Korean Soc. Clin. Toxicol. 11(2):133-135 (in Korean).
17 Soltis, D.E., P.S. Soltis, D.L. Nickrent, L.A. Johnson, W.J. Hahn, S.B. Hoot, J.A. Sweere, R.K. Kuzoff, K.A. Kron, M.W. Chase, S.M. Swensen, E.A. Zimmer, S.M. Chaw, L.J. Gillespie, W.J. Kress and K.J. Sytsma. 1997. Angiosperm phylogeny inferred from 18S ribosomal DNA sequences. Ann. Mo. Bot. Gard. 84(1):1-49.   DOI
18 Coyle, H.M. 2004. Forensic Botany: Principles and Applications to Criminal Casework. CRC Press, FL (USA). pp. 97-115.
19 Chang, M., J.Y. Kim, H. Lee, E.J. Lee and S. Moon. 2021. Constructing a DNA barcode database and identification of marker nucleotides for forensic discrimination of opium poppy in South Korea. Journal of Science Criminal Investigation 15(1):7-23 (in Korean).   DOI
20 Lee, E.J., S.H. Moon, H.C. Park, J.Y. Lee, M.H. Kim, J.W. Lee, J.Y. Jung, J.S. Seo, S.K. Lim and Y.H Lee. 2018. The species identification of botanical stains from a suspect's clothes using plastid DNA markers for a criminal case. Korean Journal of Forensic Science 19(1):36-42 (in Korean).
21 Luo, K.C.S., K.L. Chen, J.Y. Song and H. Yao. 2009. Application of DNA barcoding to the medicinal plants of the Araceae family. Planta Med. 75(04):416.
22 Yoon, C.Y., K.W. Park, J. Jung, J.Y. Hyun and J.H. Kim. 2019. A report on the taxonomic characters, ecological risk and weed risk assessment of un-introduced plants which are designated in law by the Ministry of Environment in Korea as environmentally harmful species (I). Korean J. Plant Res. 32(5):543-558.
23 China Plant BOL Group, D.Z. Li, L.M. Gao, H.T. Li, H. Wang, X.J. Ge, J.Q. Liu, Z.D. Chen, S.L. Zhou, S.L. Chen, J.B. Yang, C.X. Fu, C.X. Zeng, H.F. Yan, Y.J. Zhu, Y.S. Sun, S.Y. Chen, L. Zhao, K. Wang, T. Yang and G.W. Duan. 2011. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. PNASU. 108(49):19641-19646.   DOI
24 Stech, M., D. Quandt and W. Frey. 2003. Molecular circumscription of the hornworts (Anthocerotophyta) based on the chloroplast DNA trnL-trnF region. J. Plant. Res. 116(5):389-398.   DOI
25 Taberlet, P., L. Gielly, G. Pautou and J. Bouvet. 1991. Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant. Mol. Biol. 17:1105-1109.   DOI
26 Tate, J.A. and B.B. Simpson. 2003. Paraphyly of Tarasa (Malvaceae) and diverse origins of the polyploid species. Syst. Bot. 28(4):723-737.
27 Yao, H., J. Song, C. Liu, K. Luo, J. Han, Y. Li, P. Xiaohui, X. Hongxi, Z. Yingjie, X. Peigen and S. Chen. 2010. Use of ITS2 region as the universal DNA barcode for plants and animals. PloS One 5(10):e13102.   DOI
28 Guo, L., Z. Sui, S. Zhang, Y. Ren and Y. Liu. 2015. Comparison of potential diatom 'barcode'genes (the 18S rRNA gene and ITS, COI, rbcL) and their effectiveness in discriminating and determining species taxonomy in the Bacillariophyta. Int. J. Syst. Evol. Micr. 65(4):1369-1380.   DOI
29 Korea National Arboretum. 2021. Filed Guide to Poisonous Plants of Korea: Spring. Korea National Arboretum, Pocheon, Korea. pp. 1-142.
30 Kress, W.J. and D.L. Erickson. 2007. A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS One 2(6):e508.   DOI
31 Jin, W.J., T.O. Jeong, J.B. Lee and Y.H. Jin. 2004. Case: Phytolacca radix poisoning due to misidentification as an arrowroot. J. Korean Soc. Clin. Toxicol. 2(1):27-30 (in Korean).
32 Ha, J.M., J.I. Jeong, S.S. Min and S.K. Lee. 2012. The species identification from vegetable materials using species-specific genetic markers for food forensics. Korean Journal of Forensic Science 13(1):90-92 (in Korean).
33 Hwang, J.Y. and Kim H. 2015. Case report: A patient presenting with elevations of cardiac enzyme levels after Veratrum oxysepalum ingestion. J. Korean Soc. Clin. Toxicol. 13(2):111-116 (in Korean).
34 Korea National Arboretum. 2020. Filed Guide to Poisonous Plants of Korea: Autumn. Korea National Arboretum, Pocheon, Korea. pp. 1-123.
35 Fazekas, A.J., K.S. Burgess, P.R. Kesanakurti, S.W. Graham, S.G. Newmaster, B.C. Husband, D.M. Percy, M. Hajibabaei and S. C. Barrett. 2008. Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PloS One 3(7):e2802.   DOI
36 Hollingsworth, P.M., S.W. Graham and D.P. Little. 2011. Choosing and using a plant DNA barcode. PloS One 6(5):e19254.   DOI