• Tuberculosis and Respiratory Diseases
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• v.60 no.2
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• pp.151-159
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• 2006

Background : Excision repair cross complementing gene 1 (ERCC1) not only has a protective role against carcinogens, but plays an important role in cisplatin-resistance via the repair of cisplatin-DNA adducts. This study investigated the association between the ERCC1 expression levels in sputum and survival after cisplatin-based chemotherapy in patients with inoperable non-small cell lung cancer (NSCLC). Methods : Using the sputum collected from 67 inoperable (stage IIIa-IV) NSCLC patients treated with either taxanes (33 cases) or gemcitabine (34 cases) plus cisplatin, the relative expression levels of ERCC1 and the expression of the tumor specific antigen, MAGE, were examined by the quantitative RT-PCR and RT-PCR, respectively. The response and survival were compared with the relative level of ERCC1 or MAGE expression and the treatment modality. Results : In the sputum, ERCC1 and MAGE was detected in 74.6% and 40.2% of patients, respectively. Using the median ERCC1 level, the patients were classified as having high or low ERCC1 expression. The median overall survival (MST) was significantly longer in patients with a high ERCC1 expression level than those with a low expression level (84 weeks vs. 44 weeks respectively, P=0.017). In the taxene-based treatment group, the MST was longer than the gemcitabine group (79 weeks vs. 47 weeks, respectively, P=0.03). The levels of ERCC1 were significantly higher in patients who were MAGE-positive (P=0.003). In the MAGE-negative patients, the MST was longer in the high ERCC1 group (103 weeks vs. 43 weeks, P=0.008), but not in the MAGE-positive patients (62 weeks vs. 44 weeks, P=0.348). Conclusion : ERCC1 expression in the sputum can be a prognostic factor for survival after chemotherapy in patients with inoperable NSCLC.

• BMB Reports
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• v.45 no.7
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• pp.408-413
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• 2012

Almost all melanoma cells express at least one member of the MAGE-A antigen family, making the cytotoxic T cells (CTLs) epitopes with cross-immunizing potential in this family attractive candidates for the broad spectrum of anti-melanoma immunotherapy. In this study, four highly homologous peptides (P264: FLWGPRALA, P264I9: FLWGPRALI, P264V9: FLWGPRALV, and P264H8: FLWGPRAHA) from the MAGE-A antigens were selected by homologous alignment. All four peptides showed high binding affinity and stability to HLA-A$^*02:01$ molecules, and could prime CTL immune responses in human PBMCs and in HLA-A$^*02:01/K^b$ transgenic mice. CTLs elicited by the four epitope peptides could cross-lyse tumor cells expressing the mutual target antigens, except MAGE-A11 which was not tested. However, CTLs induced by P264V9 and P264I9 showed the strongest target cell lysis capabilities, suggesting both peptides may represent the common CTL epitopes shared by the eight MAGE-A antigens, which could induce more potent and broad-spectrum antitumor responses in immunotherapy.

• Journal of Fashion Business
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• v.6 no.1
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• pp.31-42
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• 2002

The purpose of this study is research on the characteristics of post-modernism expressed on western hair styles in the late 20th century. Therefore this thesis analyzed the relationship of modernism & the hair styles of that mage and post-modernism & the hair styles of that mage. That will re-illuminate the same beauty worth of same age as to accurate the concept of post-modernism & the hair styles of that mage. And the last, criticizing the post-modernism design and that hair design, helping to find new trends. The method of this study was the literature research & analysis of visual materials focus on upper bust image that can know the relationship of modernism & the hair styles of that mage, and post-modernism & the hair styles of that mage. First, the literature research is composed of analysis of theses, magazines on art, clothing and cosmetology having the contents of 20th century western culture, art and hair styles. Second, the analysis of visual materials is composed of analysis of video films, slide films and photographs of books, magazines, and internet home-pages. Modern western hair styles, appeared after 1910's, is represented as a short cut & permanent wave. That express experimentalism, functionalism, and purism, that is same as the other art's trend of same age. Post-modern western hair styles, appeared after 1960's, also show the same trend with same age's. This summarized & characterized 8 categories; historical method, pluralism, negotiation, escape from the formality, recovery of humanity, ornament, connection with race, metaphor & symbolism. But post-modernism concentrating pluralism makes easy making low quality art and absence of criticism, so this thesis criticised it 5 categories; absence of ideas, absence of the social sense of responsibility, lost of one's characteristics, decline of skills, decline of worth of usages.

• The KIPS Transactions:PartD
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• v.16D no.5
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• pp.643-650
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• 2009

As one of typical techniques for acquiring bio-information, microarray has contributed greatly to development of bioinformatics. Although it is established as a core technology in bioinformatics, it has difficulty in sharing and storing data because data from experiments has huge and complex type. In this paper, we propose a new method which uses the feature that microarray data format in MAGE-ML, a standard format for exchanging data, has frequent structurally similar patterns. This method constructs compact database by simplifying MAGE-ML schema. In this method, Inlining techniques and newly proposed classification techniques using structural similarity of elements are used. The structure of database becomes simpler and number of table-joins is reduced, performance is enhanced using this method.

• Proceedings of the Korean Information Science Society Conference
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• 2003.10a
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• pp.472-474
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• 2003

그리드는 다양한 기관들에 의해 소유되고 관리되는 컴퓨팅 자원들을 통합적이며 협력적으로 사용하게 하는 기반 구조이다. 이러한 기반 구조를 제공하기 위해서 Globus, Legion, Condor 등 많은 시스템이 개발되어 왔고 현재도 계속 개발 중에 있다. 하지만 이런 시스템은 시스템의 경직성 때문에 다양한 환경에 적용시키기가 어렵다. 본 논문에서는 이러한 문제점을 보완하기 위해 기존의 시스템에 유연성을 제공하는 모듈 기반의 적응형 그리드 환경(Module-based Adaptive Grid Environment)을 개발하였다. 이에 개발자는 MAGE를 이용하여 유연하게 응용 프로그램을 개발할 수 있으며 사용자는 MAGE를 이용하여 자신이 사용하는 환경에 맞게 그리드를 사용할 수 있다. 더 나아가 MAGE를 사용하여 수시로 변화는 환경에 적응하여 반응하는 자기 반영적인 미들웨어를 제공할 수 있다.

• Journal of Astronomy and Space Sciences
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• v.39 no.3
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• pp.79-86
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• 2022

Thermospheric wind observations from high to mid latitudes are compared with the newly developed Multiscale Atmosphere Geospace Environment (MAGE) model for the Nov 3-4 geomagnetic storm. The observation and simulation comparison shows a very good agreement and is better at high latitudes in general. We were able to identify a thermospheric poleward wind reduction possibly linked to a northward turning of the Interplanetary Magnetic Field (IMF) at ~22 UT on Nov 3 and an enhancement of the poleward wind to a southward turning near 10 UT on Nov 4 at high latitudes. An IMF southward turning may have led to an enhancement of equatorward winds at Boulder, Colorado near midnight. Simultaneous occurrence of aurora may be associated with an IMF By turning negative. The MAGE model wind simulations are consistent with observations in these cases. The results show the model can be a very useful tool to further study the magnetosphere and ionosphere coupling on short time scales.

• Journal of Biomedical Engineering Research
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• v.10 no.2
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• pp.102-105
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• 1989

In electrical impedance tomography(EIT), we use boundary current and voltage measurements toprovide the information about the cross-sectional distribution of electrical impedance or resistivity. One of the major problems in EIT has been the inaccessibility of internal voltage or current data in finding the internal impedance values. We propose a new image reconstruction method using internal current density data measured by NMR. We obtained a two-dimensional current density distribution within a phantom by processing the real and imaginary MR images from a 4.77 NMR machine. We implemented a resistivity mage reconstruction algorithm using the finite element method and sensitivity matrix. We presented computer simulation results of the mage reconstruction algorithm and furture direction of the research.

• Journal of Chest Surgery
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• v.37 no.12
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• pp.967-977
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• 2004

Background: DNA methylation is one of the important gene expression mechanisms of the cell. When cytosine of CpG dinucleotide in promotor is hypomethylated, expression of some genes that is controlled by this promoter is altered. In this study, the author investigated the effect of DNA demethylating agent, 5-aza-2'-deoxycytidine (ADC), on the expressions of cancer antigen genes, MHC and B7 in 4 lung cancer cell lines, NCIH1703, NCIH522, MRC-5, and A549. Material and Method: After treatment of cell lines, NCIH1703, NCIH522, MRC-5 and A549 with ADC (1 uM) for 48 hours, RT-PCR was performed by using the primers of MAGE, GAGE, NY-ESO-1, PSMA, CEA, and SCC antigen gene. In order to find the optimal ADC treatment condition for induction of cancer antigen, we studied the effect of ADC treatment time and dose on the cancer antigen gene expression. To know the effect of ADC on the expression of MHC or B7 and cell growth, cells were treated with 1 uM of ADC for 72 hours for FACS analysis or cells were treated with 0.2, 1 or 5 uM of ADC for 96 hours for cell counting. Result: After treatment of ADC (1 uM) for 48 hours, the expressions of MAGE, GAGE, NY-ESO-1, and PSMA genes increased in some cell lines. Among 6 MAGE isotypes tested, and gene expression of MAGE-1, -2, -3, -4 and -6 could be induced by ADC treatment. However, CEA gene expression did not change and SCC gene expression was decreased by ADC treatment. Gene expression was generally induced 24 - 28 hours after ADC treatment and expression of MAGE, GAGE, and NY-ESO-1 was maintained at least 14 days after ADC ADC teatment, and expression of MAGE, GAGE, and NY-ESO-1 was maintained at least 14 days after ADC teatment in ADC-Free medium. Most gene expression could be induced at 0.2 uM of ADC, but gene expression increased dependently on ADC treatment dose. The expression of MHC and B7 was not increased by ADC treatment in all four cell lines, and the growth rate of 4 cell lines decreased significantly with the increase of ADC concentrations. Conclusion: Treatment of lung cancer cell lines with ADC increases the gene expression MAGE, GAGE and NY-ESO-1 that are capable of induction of cytotoxic T lymphocyte response. We suggest that treatment with 1 uM of ADC for 48 hours and then culturing in ADC-free medium is optimal condition for induction of cancer antigen. However, ADC has no effect on MHC and B7 induction, additional modification for increase of expression of MHC, B7 and cytokine will be needed for production of efficient cancer cell vaccine.

• The Journal of the Korea Contents Association
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• v.9 no.8
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• pp.18-31
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• 2009

Improved reliability of microarray data and its reproducibility lead to recent increment in demand of data sharing and utilization among laboratories, but house-keeping and publicly opened microarray experimental data can hardly be accessed and utilized since they are in heterogeneous formats according to the various experimental methods and microarray platforms. In this paper, we propose a microarray sharing method which can easily retrieve and integrate microarray data from different experiment platforms, data formats, normalization methods, and analysis methods. Our system is based on web-service technology. The biologists of each site are able to search UDDI(Universal Description, Discovery, and Integration) registry, and download microarray data with common data structure of standard format recommended by MGED(Microarray Gene Expression Databases) society. The common data structure defined in this paper consists of IDF(Investigation Design Format), ADF(Array Design Format), SDRF(Sample and Relationship Format), and EDF(Expression Data Format). These components play role as templates to integrate microarray data with various structure and can be stored in standard formats such as MAGE-ML, MAGE-TAB, and XML Schema. In addition, our system provides advanced tools of automatic microarray data submitter and file manager to manipulate local microarray data efficiently.

• Journal of Chest Surgery
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• v.53 no.1
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• pp.22-27
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• 2020

Background: Previous studies have shown that lung cancer stem cells express CD133 and that certain cancer stem cells express cancer germline antigens (CGAs). The transcriptional regulation of CD133 is complicated and poorly understood. We investigated CD133 and CGA expression in a non-small cell lung cancer cell line. Methods: The expression levels of CD133 and CGAs (MAGE-6, GAGE, SSX, and TRAG-3) were measured in an NCI-H292 lung cancer cell line. The methylation status of the CD133 gene promoter region was analyzed. The expression levels and promoter methylation statuses of CD133 and CGAs were confirmed by treatment with the demethylating agent 5-aza-2'-deoxycytidine (ADC). Results: After treatment with ADC, CD133 expression was no longer detected. MAGE-6 and TRAG-3 were detected before ADC treatment, while GAGE and SSX were not detected. ADC treatment upregulated MAGE-6 and TRAG-3 expression, while GAGE expression was still undetected after treatment, and only weak SSX expression was observed. GAGE expression was not correlated with expression of CD133, while the levels of expression of MAGE-6, TRAG-3, and SSX were inversely correlated with CD133 expression. Conclusion: These results showed that CD133 expression can be regulated by methylation. Thus, the demethylation of the CD133 promoter may compromise the treatment of lung cancer by inactivating cancer stem cells and/or activating CGAs.