• 한국산학기술학회논문지
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• 제12권12호
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• pp.5709-5714
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• 2011

이 연구는 뇌성마비 아동의 기능적 수준 분류 체계의 유용성을 알아보기 위해 실시되었다. 이를 위해 2008년 9월부터 2010년 8월까지의 기간 동안 뇌성마비 아동 217명을 대상으로 대동작 기능 분류체계(GMFCS), 손 기능 분류 체계(MACS), 일상생활 활동을 측정하고 도구 간의 관계를 알아보았다. 그 결과, 대동작 기능 분류체계와 일상 생활 활동은 모든 하위 영역 및 총점과 유의한 상관이 있는 것으로 나타났다(p<.05). 손 기능 분류체계와 일상생활 활동은 이상운동형 아동에서 일상생활 하위 영역 중 의사소통과 상관이 유의하지 않은 것을 제외하고 모든 하위 영역 및 총점과 유의한 상관이 있는 것으로 나타났다(p<.05). GMFCS와 MACS의 관계를 알아본 결과, 가장 많은 분포를 나타낸 것은 GMFCS의 경우 1수준(20.3%)과 5수준(40.6%)이었고, MACS의 경우는 2수준(48.8%)과 5수준(16.6%)이었다. 결론적으로, 뇌성마비 아동의 기능적 수준 분류 체계인 GMFCS와 MACS는 임상적으로 유용한 평가 체계로 사용될 수 있을 것으로 생각된다.

• 전자공학회논문지C
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• 제35C권5호
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• pp.25-32
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• 1998

In this paper, SA (shape adaptive)-DCT is implemented using a datapath with 4 MACs (multiplication & accumulator). DCT is a well-known bottleneck of real-time video compression using MPEG-like schemes. High-speed pipelined MACs presented here implement real-time DCT. A datapath in this paper executes DCT/IDCT algorithms for QCIF 15fps(frame per second), maximum rate of VLBV(very low bitrte video) in MPEG-4. A 32bit accumulator in a MAC prevents distortion caused by fixed-point process. It can be applied to various operations such as ME (motion estimation) and MC(motion compensation) with a absolutor and a halfer.

• 한국음향학회:학술대회논문집
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• 한국음향학회 1995년도 제12회 음성통신 및 신호처리 워크샵 논문집 (SCAS 12권 1호)
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• pp.137-140
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• 1995

In the paper, CDMA's vocoder algorithm, QCELP, was analyzed. And, 16-bit programmable DSP core for QCELP was designed. When it is used two MACs in DSP, we can implement low-power DSP and estimate decrease of parameter computation speed. Also, we implemented in FIFO memory using register file to increase the access time of the data. This DSP was designed using logic synthesis tool, COMPASS, by top-down design methodology. Therefore, it is possible to cope with rapid change at mobile communication market.

• 한국전문물리치료학회지
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• 제21권3호
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• pp.55-62
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• 2014

This study investigated the relationship between function classification systems and the Pediatric Evaluation of Disability Inventory (PEDI) functional skills in children with cerebral palsy (CP). Two hundred and eleven children with CP participated in this study. The Korean-Gross Motor Function Classification System (K-GMFCS), Korean-Manual Ability Classification System (K-MACS), Korean-Communication Function Classification System (K-CFCS), and self-care, mobility, and social function domains of the Korean-Pediatric Evaluation of Disability Inventory (K-PEDI) functional skills were measured by physical therapists or occupational therapists. All of the function classification systems were significantly correlated with PEDI functional skills ($r_s$=-.549 to -.826) (p<.05). Especially, K-GMFCS, K-MACS, and K-CFCS were correlated significantly with mobility, self-care, and social function, respectively. Using stepwise multiple regression analysis, we established that K-GMFCS, K-MACS, and K-CFCS were predictors of self-care skills (74.3%) and mobility skills (79.5%) of the K-PEDI (p<.05). In addition, K-CFCS and K-MACS were predictors of social function (65.9%) of the K-PEDI (p<.05). The information gathered in this study using the levels measured in the function classification systems may be useful to clinicians for estimating the PEDI functional skills in children with CP.

• Mycobiology
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• 제45권4호
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• pp.401-408
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• 2017

Colletotrichum gloeosporioides is an economically important fungal pathogen causing substantial yield losses indifferent host plants. To understand the genetic diversity and molecular epidemiology of this fungus, we have developed a novel, high-resolution multi-locus microsatellite typing (MLMT) method. Bioinformatic analysis of C. gloeosporioides unannotated genome sequence yielded eight potential microsatellite loci, of which five, CG1 $(GT)_n$, CG2 $(GT1)_n$, CG3 $(TC)_n$, CG4 $(CT)_n$, and CG5 $(CT1)_n$ were selected for further study based on their universal amplification potential, reproducibility, and repeat number polymorphism. The selected microsatellites were used to analyze 31 strains of C. gloeosporioides isolated from 20 different host plants from India. All microsatellite loci were found to be polymorphic, and the approximate fragment sizes of microsatellite loci CG1, CG2, CG3, CG4, and CG5 were in ranges of 213-241, 197-227, 231-265, 209-275, and 132-188, respectively. Among the 31 isolates, 55 different genotypes were identified. The Simpson's index of diversity (D) values for the individual locus ranged from 0.79 to 0.92, with the D value of all combined five microsatellite loci being 0.99. Microsatellite data analysis revealed that isolates from Ocimum sanctum, Capsicum annuum (chili pepper), and Mangifera indica (mango) formed distinct clusters, therefore exhibited some level of correlation between certain genotypes and host. The developed MLMT method would be a powerful tool for studying the genetic diversity and any possible genotype-host correlation in C. gloeosporioides.

• 한국수정란이식학회지
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• 제33권4호
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• pp.245-254
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• 2018

Pigs are considered as optimal donor animal for the successful xenotransplantation. To increase the possibility of clinical application, genetic modification to increase compatibility with human is an important and essential process. Genetic modification technique has been developed and improved to produce genetically modified pigs rapidly. CRISPR/Cas9 system is widely used in various fields including the production of transgenic animals and also can be enable multiple gene modifications. In this study, we developed new gene targeting vector and enrichment system for the rapid and efficient selection of genetically modified cells. We conducted co-transfection with two targeting vectors for simultaneous inactivation of two genes and enrichment of the genetically modified cells using MACS. After this efficient enrichment, genotypic analysis of each colony showed that colonies which have genetic modifications on both genes were confirmed with high efficiency. Somatic cell nuclear transfer was conducted with established donor cells and genetically modified pigs were successfully produced. Genotypic and phenotypic analysis of generated pigs showed identical genotypes with donor cells and no surface expression of ${\alpha}$-Gal and HD antigens. Furthermore, functional analysis using pooled human serum revealed dramatically reduction of human natural antibody (IgG and IgM) binding level and natural antibody-mediated cytotoxicity. In conclusion, the constructed vector and enrichment system using MACS used in this study is efficient and useful to generate genetically modified donor cells with multiple genetic alterations and lead to an efficient production of genetically modified pigs.

• 한국동물생명공학회지
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• 제36권2호
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• pp.91-98
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• 2021

The objective of this study was to establish a selection process for high quality sperm in bovine semen using sperm separation by magnetic activation (MACS). For this, semen from 21 Nellore bulls was collected using an artificial vagina. To guarantee the presence of pathologies in the ejaculate, animals previously declassified in four consecutive spermiogram were used. Semen was analyzed in five statuses: (1) fresh semen (fresh); (2) density gradient centrifugation (DGC), percoll column; (3) non-apoptotic fraction after separation by MACS (MAC); (4) apoptotic fraction from the separation (MACPOOR); and (5) MAC followed by DGC (MACDGC). Using a computerized analysis system (CASA), motility was measured. The sperm morphology was evaluated by phase contrast, and the supravital test was completed with eosin/nigrosin staining. For DGC, 20 × 10⁶ cells were used in a gradient of 90% and 45% percoll. MACS used 10 × 10⁶ cells with 20 μL of nanoparticles attached to annexin V, and filtered through the MiniMACS magnetic separation column. Membrane integrity was assessed with SYBR-14/IP and mitochondrial potential with JC-1 by flow cytometry. Processing sperm by MACDGC, was more effective in obtaining samples with high quality sperm, verified by the total of abnormalities in the samples: 35.04 ± 2.29%, 21.50 ± 1.47%, 17.30 ± 1.10%, 30.68 ± 1.94% and 10.50 ± 1.46%, respectively for fresh, DGC, MAC, MACPOOR, and MACDGC. The subpopulation of non-apoptotic sperm had a high number of live cells (82.65%), membrane integrity (56.60%) and mitochondrial potential (83.98%) (p < 0.05). These findings suggest that this nanotechnological method, that uses nanoparticles, is efficient in the production of high-quality semen samples for assisted reproduction procedures in cattle.

• 정보처리학회논문지C
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• 제19C권3호
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• pp.173-178
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• 2012

블룸필터를 이용하면 다수의 MAC을 표현하기 위해 요구되는 메시지의 길이를 줄일 수 있다고 주장하는 두 논문이 최근에 발표되었다. 하지만 이 기법들은 보안성을 고려하지 않고 메시지의 길이만 비교한 것으로 분석되었다. MAC은 보안을 목적으로 하는 코드이기 때문에 다수의 MAC과 이들을 표현한 블룸필터가 동등한 보안수준을 가지도록 하고 메시지의 길이를 비교해야 한다. 이 논문에서는 블룸필터와 압축블룸필터, 그리고 다수의 MAC이 동등한 보안수준을 가질 때의 메시지 길이를 분석하여 보인다.

• 대한물리의학회지
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• 제12권2호
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• pp.67-74
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• 2017

PURPOSE: The purpose of this study was to investigate the difference and relationship between sensory processing abilities, gross motor and fine motor capabilities in children with cerebral palsy. METHODS: 104 children with cerebral palsy participated in the study. Sensory processing abilities of the subjects were measured by Short Sensory Profile (SSP). Gross and fine motor abilities were each measured using the Gross Motor Function Classification System (GMFCS) and Manual Ability Classification System (MACS), respectively. RESULTS: There were significant correlations between SSP level and GMFCS (R=.72, p<.00) or MACS (R=.77, p<.00) levels. Significant differences were showed each gross motor (p=.01) and fine motor level (p=.00) among sensory processing level of children. In addition, sub-items of sensory processing as Tactile sensitivity, Movement sensitivity, Auditory filtering and Low energy/Weak were significantly were showed significant correlations gross motor and fine motor level (p=.01). Also, multiple regression result was showed that as MACS level and GMFCS level were higher, the SSP total score was higher all of participants (adjusted $R^2=.62$). CONCLUSION: Sensory processing abilities of children with cerebral palsy were related with gross motor and fine motor capabilities. Also gross motor and fine motor capabilities are as higher, the sensory processing skill was well of cerebral palsy.

• 대한족부족관절학회지
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• 제18권3호
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• pp.100-107
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• 2014

Purpose: The purpose of this study is to evaluate the efficacy of mesenchymal stem cell (MSC) isolation by the magnetic-activated cell sorting (MACS) method in tendon tissue-derived cells compared to the colony picking method for isolation of MSCs by picking colony-forming cells. Materials and Methods: Human tendon-derived cells were isolated by enzyme digestion using normal tendon tissues from three donors. We used the magnetic kit and well-known MSC markers (CD90 or CD105) to isolate MSCs in tendon-derived cells using MACS. Cloning cylinders were used to isolate colony-forming cells having MSC characteristics in tendon-derived cells. Colony-forming unit-fibroblast (CFU-F) assay was used to evaluate the self-renewal capacity of cells isolated using the colony picking method or MACS. For comparison of differentiation potentials into osteogenic or adipogenic lineage between two groups, alizarin red S and oil red O staining were performed at 14 days after induction of differentiation in vitro. Results: Flow cytometry results showed that early passage tendon-derived cells expressed CD44 in 99.13%, CD90 in 56.51%, and CD105 in 86.19%. In the CFU-F assay, CD90+ or CD105+ cells isolated with MACS showed larger colony formation in size than cells isolated using the colony picking method. We also observed that CD90+ or CD105+ cells were constantly differentiated into both osteogenic and adipogenic lineages in cells from all donors, whereas cells isolated using the colony picking method were heterogeneous in differentiation potentials to the osteogenic and adipogenic lineages. Conclusion: CD90+ or CD105+ cells isolated using MACS showed superior MSC characteristics in the self-renewal and multi-differentiation capacities compared with cells isolated using the colony picking method.