• Title/Summary/Keyword: M5(R)

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Irrigation Control for Improving Irrigation Efficiency in Coir Substrate Hydroponic System (코이어 배지 수경재배에서 관수효율 향상을 위한 급액 제어)

  • Yoo, Hyung-Joo;Choi, Eun-Young;Lee, Yong-Beom
    • Journal of Bio-Environment Control
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    • v.24 no.3
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    • pp.153-160
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    • 2015
  • The objectives of this study were to determine optimal length of off-time between irrigation cycles to improve irrigation efficiency using a frequency domain reflectometry (FDR) sensor-automated irrigation (FAI) system for tomato (Solanum lycopersicum L.) cultivation aimed at minimizing effluent from coir substrate hydroponics. For treatments, the 5-minute off-time length between 3-minute run-times (defined as 3R5F), 10-minute off-time length between 3-minute run-times (defined as 3R10F), or 15-minute off-time length between 5-minute run-times (defined as 5R15F) were set. During the 3-minute or 5-minute run-time, a 60mL or 80mL of nutrient solution was irrigated to each plant, respectively. Until 62 days after transplant (DAT) during the autumn to winter cultivation, daily irrigation volume was in the order of 3R5F (858mL) > 5R15F (409mL) > 3R10F (306mL) treatment, and daily drainage ratio was in the order of 3R5F (44%) > 5R15F (23%) > 3R10F (14%). Between 63 and 102 DAT, daily irrigated volume was in the order of 5R15F (888mL) > 3R5F (695mL) > 3R10F (524mL) with the highest drainage ratio, 19% (${\pm}2.6$), at the 5R15F treatment. During the spring to summer cultivation, daily irrigation volume and drainage ratio per plant was higher in the 3R5F treatment than that of the 3R10F treatment. For both cultivations, a higher water use efficiency (WUE) was observed under the 3R10F treatment. Integrated all the data suggest that the optimal off-time length is 10 minutes.

Overproduction of Bacterial Trypsin in Streptomyces - Optimization for Streptomyces griseus Trypsin Production by Recombinant Streptomyces (미생물을 이용한 트립신 과대 생산 연구 - Streptomyces용 숙주-벡터계를 이용한 트립신 유전자의 대량발현 최적화 -)

  • Kim, Jong-Hee;Hong, Soon-Kwang
    • Microbiology and Biotechnology Letters
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    • v.36 no.1
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    • pp.28-33
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    • 2008
  • The expression vector (pWHM3-TR1R2) for sprT gene encoding Streptomyces griseus trypsin (SGT) followed by two regulatory genes, sgtR1 and sgtR2, was introduced into Streptomyces lividans TK24 and Streptomyces griseus IFO 13350. Various media with different compositions were used to maximize the productivity of SGT in the recombinant trains. he SGT productivity was best when the transformant of S. lividans TK24 was cultivated in R2YE medium (0.74 unit/mL) at 5 days of cultivation. C5/L (0.66 unit/mL) medium also gave a good productivity, but Livid (0.08 unit/mL) and NDSK (0.06 unit/mL) yielded poor productivities. S. griseus IFO 13350/pWHM3-TR1R2 produced SGT by 1.518 unit/mL (C5/L), 1.284unit/mL (R2YE),0.932 unit/mL (NDSK), and 0.295 unit/mL (Livid) at 7 days of cultivation, which was much higher than those from S. lividans TK24/TR1R2. The SGT protein was purified from the culture broth of S. griseus IFO 13350/pWHM3-TR1R2 in C5/L to homogeneity via ammonium sulfate fractionation, and CM-sepharose and SP-sepharose column chromatographies. The specific activity of purified SGT was 69,252 unit/mg, and the final purification fold and recovery yield were 6.5 and 1.4%, respectively.

The Study About Attenuation of Scatter Ray According to Distance Inverse Square Law at General Projection (일반촬영 시 거리역자승법칙에 따른 산란선 감약에 관한 연구)

  • Jeon, Min-Cheol;Lim, Hyun-Soo;Han, Man-Seok
    • Journal of radiological science and technology
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    • v.34 no.3
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    • pp.183-188
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    • 2011
  • We studied the optimal location where the radiation dose of radiological technologists is minimally measured. The measured scatter dose has been compared with the distance inverse square law. We measured the primary X-ray with different tube conditions (60, 70, 81 and 90 kVp) and distances (60, 120 and 180 cm). The scatter ray has been measured with various locations (42.5, 52.4 and 62.4 cm for front and back side, 0 to 60 cm with 10 cm interval for left and right side). The results of this study showed that the dose of primary X-ray was attenuated to 20.52 (27.20%), 28.58 (25.20%), 38.82 (26.32%) and 48.20 mR (26.27%) for each tube voltages at 120 cm. In addition, The dose were 7.06 (8.91%), 9.90 (8.73%), 13.64 (9.25%) and 16.60 mR (9.05%) at 180 cm. As for the scatter in front and back side, the attenuated dose were 0.15 mR (23.09%) and 0.15 mR (22.08%) at 120 cm, and 0.07 mR (10.43%) and 0.06 mR (8.83%) at 180 cm. Scatter was decreased in third quadrant. Therefore, it is recommended that radiological technologists should keep long distance from the object.

Synthesis and Characterization of New Group 13 Complexes of 2-Acetylpyridine-S-methyldithiocarbazate. Single-Crystal Structure of Me₂Ga[$NC_5H_4C$(CH₃)NNC(S)SMe] and Me₂In[$NC_5H_5C$(CH₃)NNC(S)SMe]

  • 백철기;강상욱;이채호;이영행;고재정
    • Bulletin of the Korean Chemical Society
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    • v.18 no.3
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    • pp.311-316
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    • 1997
  • The synthesis and characterization of the mononuclear group 13 heterocyclic carboxaldehyde methyldithiocarbazate complexes Me2M[NC5H4CRNNC(S)SCH3] (M=Al, R=H(1); M=Ga, R=H(2); M=Al, R=CH3(3); M-Ga, R=CH3(4); M=In, R=CH3(5)) are described. Compounds 1-5 were prepared by the reaction of MMe3 (M=Al, Ga, In) with 2-formy or 2-acetylpyridine-S-methyldithiocarbazate in toluene. These compounds 1-5 have been characterized by microanalysis, NMR (1H, 13C) spectroscopy, mass spectra, and single-crystal X-ray diffraction. X-ray single-crystal diffraction analyses reveal that 4-5 are mononuclear metal compounds with coordination number of 5 and N,N,S coordination mode.

Syntheses of Anilide Derivatives from Amino Acids ad Their Biological Activities (Ⅰ). Preparation of (R)-2-Pyrrolidine-5-carbox-anilide Derivatives and Their Effects on the Germination of Plant Seeds (Amino酸으로부터 Anilide誘濤體의 合成과 生理活性에 關한 硏究 (第1報). (R)-2-Pyrrolidone-5-carbox-anilide 誘濤體의 合成과 植物種子發芽에 미치는 影響)

  • Chun-Soo Lee
    • Journal of the Korean Chemical Society
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    • v.25 no.1
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    • pp.38-43
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    • 1981
  • Pyrrolidone-anilide derivatives from L-glutamic acid and anilines were synthesized as follows: The products were identified by elementary analysis, IR, NMR and Mass spectra with (R)-2-pyrrolidone-5-carbox-anilide, (R)-2-pyrrolidone-5-cabox-p-chloroanilide, (R)-2-pyrrolidone-5-carbox-o-toluidide, (R)-2-pyrrolidone-5-carbox-m-toluidide and (R)-2-pyrrolidone-5-carbox-p-toluidide. The products were testes for their phytotoxicity on the germination and the seedling growth of radish and rice plants. Among them, (R)-2-pyrrolidone-5-carbox-anilide and (R)-2-pyrrolidone-5-carbox-p-chloroanilide derivatives were strongly inhibitory especially on the germination and the seedling growth of radish seeds. All the compounds also showed an inhibitory activity upon the germination of rice seeds. Additionally, the inhibiting rate of radish growth differs according to the isomeric position(ortho, meta and para) of the methyl group; (R)-2-pyrrolidone-5-carbox-m-toluidide derivative was more effective than both (R)-2-pyrrolidone-5-carbox-o-toluidide and (R)-2-pyrrolidone-5-carbox-p-toluidide derivatives.

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Regulation of DREAM Expression by Group I mGluR

  • Lee, Jin-U;Kim, In-Sook;Oh, So-Ra;Ko, Suk-Jin;Lim, Mi-Kyung;Kim, Dong-Goo;Kim, Chul-Hoon
    • The Korean Journal of Physiology and Pharmacology
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    • v.15 no.2
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    • pp.95-100
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    • 2011
  • DREAM (downstream regulatory element antagonistic modulator) is a calcium-binding protein that regulates dynorphin expression, promotes potassium channel surface expression, and enhances presenilin processing in an expression level-dependent manner. However, no molecular mechanism has yet explained how protein levels of DREAM are regulated. Here we identified group I mGluR (mGluR1/5) as a positive regulator of DREAM protein expression. Overexpression of mGluR1/5 increased the cellular level of DREAM. Up-regulation of DREAM resulted in increased DREAM protein in both the nucleus and cytoplasm, where the protein acts as a transcriptional repressor and a modulator of its interacting proteins, respectively. DHPG (3,5-dihydroxyphenylglycine), a group I mGluR agonist, also up-regulated DREAM expression in cortical neurons. These results suggest that group I mGluR is the first identified receptor that may regulate DREAM activity in neurons.

Roles of Metabotropic Glutamate Receptors 1 and 5 in Rat Medial Vestibular Nucleus Neurons

  • Lee, Hae-In;Lee, Sung-Hyo;Chun, Sang-Woo
    • International Journal of Oral Biology
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    • v.36 no.2
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    • pp.71-78
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    • 2011
  • Using whole cell current- and voltage-clamp recording we investigated the characteristics and pharmacology of group I metabotropic glutamate receptor (mGluR)-mediated responses in rat medial vestibular nucleus (MVN) neurons. In current clamp conditions, activation of mGluR I by application of the group I mGluR agonist (R,S)-3,5-dihydroxyphenylglycine (DHPG) induced a direct excitation of MVN neurons that is characterized by depolarization and increased spontaneous firing frequency. To identify which of mGluR subtypes are responsible for the various actions of DHPG in MVN, we used two subtype-selective antagonists. (S)-(+)- alpha-amino-a-methylbenzeneacetic acid (LY367385) is a potent competitive antagonist that is selective for mGluR1, whereas 2-methyl-6-(phenylethynyl)-pyridine (MPEP) is a potent noncompetitive antagonist that is selective for mGluR5. In voltage clamp conditions, DHPG application increased the frequency of spontaneous and miniature inhibitory postsynaptic currents (IPSCs) but had no effect on amplitude distributions. Antagonism of the DHPG-induced increase of miniature IPSCs required the blockade of both mGluR1 and mGluR5. DHPG application induced an inward current, which can be enhanced under depolarized conditions. DHPG-induced current was blocked by LY367385, but not by MPEP. Both LY367385 and MPEP antagonized the DHPG-induced suppression of the calcium activated potassium current ($I_{AHP}$). These data suggest that mGluR1 and mGluR5 have similar roles in the regulation of the excitability of MVN neurons, and show a little distinct. Furthermore, mGluR I, via pre- and postsynaptic actions, have the potential to modulate the functions of the MVN.

IDEALS AND SUBMODULES OF MULTIPLICATION MODULES

  • LEE, SANG CHEOL;KIM, SUNAH;CHUNG, SANG-CHO
    • Journal of the Korean Mathematical Society
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    • v.42 no.5
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    • pp.933-948
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    • 2005
  • Let R be a commutative ring with identity and let M be an R-module. Then M is called a multiplication module if for every submodule N of M there exists an ideal I of R such that N = 1M. Let M be a non-zero multiplication R-module. Then we prove the following: (1) there exists a bijection: N(M)$\bigcap$V(ann$\_{R}$(M))$\rightarrow$Spec$\_{R}$(M) and in particular, there exists a bijection: N(M)$\bigcap$Max(R)$\rightarrow$Max$\_{R}$(M), (2) N(M) $\bigcap$ V(ann$\_{R}$(M)) = Supp(M) $\bigcap$ V(ann$\_{R}$(M)), and (3) for every ideal I of R, The ideal $\theta$(M) = $\sum$$\_{m(Rm :R M) of R has proved useful in studying multiplication modules. We generalize this ideal to prove the following result: Let R be a commutative ring with identity, P $\in$ Spec(R), and M a non-zero R-module satisfying (1) M is a finitely generated multiplication module, (2) PM is a multiplication module, and (3) P$^{n}$M$\neq$P$^{n+1}$ for every positive integer n, then $\bigcap$$^{$\_{n=1}$(P$^{n}$ + ann$\_{R}$(M)) $\in$ V(ann$\_{R}$(M)) = Supp(M) $\subseteq$ N(M).

Effect of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of Citrullus colocynthis (L.) Schrad

  • Ramakrishna, D.;Chaitanya, G.;Suvarchala, V.;Shasthree, T.
    • Journal of Plant Biotechnology
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    • v.45 no.1
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    • pp.55-62
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    • 2018
  • In the present study in vitro mutagenesis was used to study the effect of gamma irradiation and EMS on callus induction, morphogenesis and production of multiple shoots from different explants of Citrullus colocynthis (L.) Schrad. Gamma radiations (5 kR to 20 kR) and certain chemicals have been effected on plant growth developments and changes of biochemical metabolisms in plants. Murashige and Skoog (MS) medium containing with auxins such as NAA, IAA, 2,4-D (0.5 ~ 2.0 mg/l), cytokinines BAP, kn TDZ, (0.5 ~ 2.5 mg/l), L-Glutamic acid (1 ~ 2 mg/l) and Coconut milk (10 ~ 20%). After 5 weeks on induction media, explants and callus (EC) were exposed to 5 kR, 10 kR, 15 kR and 20kR, of gamma radiation and treated with 1, 2, 3, 4 and 5 mM ethyl methane sulphonate (EMS) for 30 min. The highest percentage of callusing was observed (70%) stem irradiated with 5 kR and significantly decrease in fresh and dry weight of callus in the below 4 kR doses and above 20 kR doses, there was a progressive decrease in the fresh weight and dry weights when compared to control callus. Maximum percentage of plantlet regeneration (59%) was induced from callus exposed to 15 kR gamma irradiation on MS media fortified with 2.0 mg/l 2,4-D + 2.0 mg/l BAP + 2.0 mg/l L-glutamic acid. Increase in gamma irradiation dose above 15 kR and 5 mM EMS reduced regeneration capacity of callus. Doses higher than 20 kR and 7 mM EMS was lethal to micropropagated plants of Citurullus colocynthis.