• 한국발생생물학회지:발생과생식
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• 제12권3호
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• pp.297-303
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• 2008

본 논문은 인공적인 단백질인 MCL-1ES BH3M에 관한 것으로 MCL-1ES BH3M를 과발현시 세포사멸을 유도한다. MCL-1L을 주형으로 재조합 PCR을 통해서 MCL-1ES BH3M를 클로닝하였다. 새롭게 클로닝한 단백질인 MCL-1ES BH3M 단백질은 안정성을 유지하기 위해서 PEST 도메인이 제거되어 있으며, 다른 BCL-2 패밀리 단백질과의 결합을 조절하기 위해서 BH3도메인의 Leu-Arg-Arg-Val-Gly-Asp-Gly 서열을 7개의 Ala 잔기로 인위적으로 돌연변이를 유도하였다. MCL-1ES BH3M를 293T 세포에서 과발현할 경우 세포사멸을 유도하였고, 항-세포사멸 단백질인 MCL-1L을 같이 과발현하더라도 세포사멸을 유도하였다. 또한, 과발현시 Caspase 9과 3를 활성화하였으며 면역염색법을 통해서 MCL-1ES BH3M 과발현시 미토콘드리아에 MCL-1ES BH3M 단백질이 부분적으로 위치하는 것을 확인하였다. 이상의 결과로 MCL-1ES BH3M는 Caspase 9과 3의 활성을 통해서 세포사멸을 유도한다. 결론적으로 본 연구는 세포사멸을 유도하는 새로운 molecule을 클로닝하였고, 이 molecule에 의한 세포사멸 기능을 확인하였다.

• 수산해양기술연구
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• 제37권1호
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• pp.24-34
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• 2001

정치망 원통의 거동을 장기선 방식으로 측정할 수 있는 계류식 수중위치측정시스템을 제작하고, 그 실용성을 확인하기 위하여 소조기 (2000. 10. 6)와 대조기 (2000. 11. 28)에 현장실험을 실시하였다. 현장실험에서 4개의 트랜스폰더는 원통의 남북방향으로 부착시키고, 1개의 트랜스폰더는 해저에 고정시켰다. 그리고, 원통까래의 상하진동은 3개의 테이터로거를 원통의 동서방향 (원통의 길이 방향)으로 부착시켜 대조기에 관측하였다. 소조기에 측정한 원통의 동서방향, 남북방향, 상하방향의 변화폭은 각각 3.2 m, 3.4 m, 2.1 m이었고, 대조기에는 각각 7.8 m, 7.8 m, 5.0 m로 소조기보다 2.3배 이상 컸다. 원통까래의 상하진동폭은 비탈그물에 가까운 쪽이 3.2 m, 중앙부가 3.7 m, 머거리쪽이 8.4 m이었다. 전반적으로 유속이 10 cm/s 이상일 때와 흐름의 방향이 원통의 길이방향과 일치하지 않을 때에 까래의 심도 감소와 상하진동이 빈번하게 발생하였다. 해저에 고정시킨 트랜스폰더를 사용하여 수파기 좌표의 변동을 측정한 결과, 정치망 사개에 부착한 수파기라도 흐름에 의해 그 좌표가 수 m 정도 변함을 알 수 있었고, 정치망의 거동 측정시 이러한 좌표의 변동을 보정하여야 측정오차를 줄일 수 있음을 알 수 있었다. 본 연구에서 제작한 3차원 수중위치측정시스템의 위치오차는 가두리에서 측정한 결과, x축, y축, z축이 각각 0.6 m, 0.8 m, 1.2 m이었고, 기선의 내부이거나 기선에 가까울수록 작았다.

• 대한수학회논문집
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• 제38권3호
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• pp.733-740
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• 2023

Let R be a commutative ring and M be an R-module. The dimension graph of M, denoted by DG(M), is a simple undirected graph whose vertex set is Z(M) ⧵ Ann(M) and two distinct vertices x and y are adjacent if and only if dim M/(x, y)M = min{dim M/xM, dim M/yM}. It is shown that DG(M) is a disconnected graph if and only if (i) Ass(M) = {𝖕, 𝖖}, Z(M) = 𝖕 ∪ 𝖖 and Ann(M) = 𝖕 ∩ 𝖖. (ii) dim M = dim R/𝖕 = dim R/𝖖. (iii) dim M/xM = dim M for all x ∈ Z(M) ⧵ Ann(M). Furthermore, it is shown that diam(DG(M)) ≤ 2 and gr(DG(M)) = 3, whenever M is Noetherian with |Z(M) ⧵ Ann(M)| ≥ 3 and DG(M) is a connected graph.

• 한국대기환경학회지
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• 제3권2호
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• pp.11-17
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• 1987

Atmospheric particulate matter (A.P.M.) was collected on quartz fiber filters from March 1985 to February 1986 at Chung-Ang University according to particle size using Andersen high-volume air smapler, and benzo (a) pyrene concentration in these particulates were analyzed by high performance liquid chromatography. The annual arithmetic mean concentration of A.P.M. was 115.50$\mug/m^3$. The annual arithmetic mean concentrations of coarse particles and fine particles in A.P.M. were 52.54$\mum/m^3$ and 62.96$\mum/m^3$ respectively. THe annual arithmetic mean concentration of benzo(a)pyrene in A.P.M. was 1.44$ng/m^3$. THe annual arithmetic mean concentrations of benzo(a)pyrene in coarse particles and fine particles were 0.05 $ng/m^3$ and 1.39 $ng/m^3$ respectively. Thus, the concentration of benzo(a)pyrene showed maldistribution of 96.53% in fine particle. A.P.M. showed wide fluctuation according to the season. The concentration of A.P.M. was lowest in summer and high in spring and winter. Coarse and fine particle concentrations in A.P.M. were highest in spring and winter, respectively. The concentrations of benzo(a)pyrene was highest in winter and lowest in summer. The concentrations of benzo(a)pyrene in fine and coarse particles were highest in winter and spring, respectively.

• Journal of Applied Biological Chemistry
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• 제43권1호
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• pp.5-11
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• 2000

Three cDNA clones encoding rice calmodulin (CaM) isoforms (OsCaM-1, OsCaM-2, and OsCaM-3) were isolated from a rice cDNA library constructed from suspension-cultured rice cells treated with fungal elicitor. The coding regions of OsCaM-1 and O.sCaM-2 were 89% homologous at DNA Ievel, whereas the 5' and 3' untranslated regions were highly divergent. The polypeptides encoded by OsCaM-1 and OsCaM-2 was identical except two conservative substitution at position 8 and 75. The coding region of OsCaM-3 was consist of a typical conserved CaM domain and an additional C-terminal extension. The amino acid sequence of conserved CaM domain of OsCaM-3 shared only 86% identity with that OsCaM-1. The OsCaM-3 cDNA is belongs to a novel group of calmodulin gene due to its C-terminal extension of 38 amino acids, a large number of which are positively charged. The extension also contains a C-terminal CaaX-box prenylation site (CVlL). Genomic Southern analysis revealed at least six copies of CaM or CaM-related genes, suggesting that calmodulin may be represented by a small multigene family in the rice geneme. Expression of OsCaM gene was examined through Northern blot analysis. Transcript level of OsCaM-3 was increased by treatment with a fungal elicitor, whereas the OsCaM-1 and OsCaM-2 genes did not respond to the fungal elicitor. The expression of OsCaM-3 gene was remarkable inhibited in the rice cells treated with cyclosporine A, calcinurin inhibitor.

• 한국가금학회지
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• 제20권1호
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• pp.11-15
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• 1993

Environmental measurements were made in 10 broiler confinement houses in order to characterize dust contaminants. Particles were collected by filters on nine stages Anderson Air Sampler, The obtained results were summarized as follows ; 1. The concentrations of the total suspended particles(TSP) were 7.584 mg/$m_3$~11.589mg/$m_3$ in 10 day old broiler houses and 15.836 mg/$m_3$~22.471 mg/$m_3$ in 30 day old broiler houses. 2. The concentrations of the coarse particles were 4.974 mg/$m_3$~18.981 mg/$m_3$ in 10 broiler confinement houses. Therefore, it was found that the coarse particles contributed to TSP between 65.6% and 85.0%. 3. The dust levels were higher in 30 day old broiler houses with TSP averaging about 19.341 mg/$m_3$ than 9.491 mg/$m_3$ in 10 day old broiler houses. In view of the above results , It was concluded that domestic broiler houses need artificial ventilation system.

• 환경위생공학
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• 제17권3호
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• pp.75-82
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• 2002

This outline survey of indoor and outdoor radon concentrations in five major cities in Korea was carried out with Electrostatic Integrating Radon Monitor(EIRM) from February to December, 1996 and January to december, 1998. The mean indoor and outdoor radon concentrations in five major cities in 1996 were $21.9{\;}Bq/\textrm{m}^3$ and $9.6{\;}Bq/\textrm{m}^3$, respectively. The mean indoor and outdoor radon concentrations in 1998 were $20.8{\;}Bq/\textrm{m}^3$ and $9.0{\;}Bq/\textrm{m}^3$, respectively. These were below the U.S.EPA radon action level. The range of indoor to outdoor radon concentrations were $0.8{\;}Bq/\textrm{m}^3{\;}~{\;}45.6{\;}Bq/\textrm{m}^3$ in 1996, $0.5{\;}Bq/\textrm{m}^3{\;}~{\;}15.2{\;}Bq/\textrm{m}^3$ in 1998, respectively. The result of our analysis showed that radon concentrations in indoor air were clearly higher than those in outdoor air. Inspection of seasonal distribute pattern indicates the enhancement during winter relative to summer.

• Environmental Analysis Health and Toxicology
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• 제24권3호
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• pp.203-211
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• 2009

A lesser degree of research is available with respect to indoor radon characteristics associated with occupants' exposure. The present study evaluated the radon levels in several public-access buildings or underground facilities, and their temporal variation in underground facilities. Radon measurements were conducted in 2005 and 2006, utilizing a continuous radon detector. A solid alpha detector (RAD7) was utilized to measure indoor radon levels. The mean radon concentrations obtained from the building or facilities were in a descending order: platforms of Daegu subway line 2, 2005 (32 $Bq/m^3$), hot-air bathroom (14 $Bq/m^3$), basement of office building (14 $Bq/m^3$), underground parking garage (14 $Bq/m^3$), underground shop (12 $Bq/m^3$), nursery (10 $Bq/m^3$), platforms of Daegu subway line 2, 2006 (9.0 $Bq/m^3$), platforms of Daegu subway line 1, 2006 (8.9 $Bq/m^3$), supermarket (7.9 $Bq/m^3$), hospital (7.3 $Bq/m^3$), and second-floor of office building (5.7 $Bq/m^3$). In general, underground-level facilities exhibited higher radon levels as compared with ground-level facilities. It was suggested that ventilation is an important parameter regarding the indoor levels of a subway. There was a decreasing or increasing trend in hourly-radon levels in a subway, whereas no trend were observed in a basement of office building. In addition, the radon levels in the subway lines 1 and 2 varied according to the platforms. The radon levels in the present study were much lower than those of previous studies. The average annual effective dose (AED) of radiation from indoor radon exposure was estimated to be between 0.043 and 0.242 mSv/yr, depending on facility types. These AEDs were substantially lower than the worldwide average AED (2.4 mSv/yr).

• 한국동굴학회:학술대회논문집
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• 한국동굴학회 1994년도 제 6차 국제학술회 ASIA 동굴 SIMPOSIUM
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• pp.125-125
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• 1994

1. 위치: 백두산 녹명봉 북쪽 4 km 되는 산비탈에 있다. 해발고도는 1900 m 로서 고산태원대에 자리 잡고 있다. 2. 형태: 동굴 어구에는 장원형으로 된 저지가 있는데 동서 길이는 4 m, 남북 길이는 3 m 이다. 이곳으로부터 2.2 m 아래에 동굴로 들어가는 통로가 있는데 길이 1.2 m, 높이 0.7 m 이고 이곳으로부터 동굴 바닥까지는 1.3 m 이다. 동굴의 크기는 높이 1-3.5 m 남북 36 m 동서 8-11 m 이고 면적은 300여 평방 m 가 된다.(중략)

• 대한화학회지
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• 제38권11호
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• pp.808-818
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• 1994

$MCl_z$(M = Mo; z = 5, M = V; z = 3)과 N,P 주개 리간드를 acetonitrile 용매에서 반응시켜 중성착물 [$MCl_3L_2$(MeCN)] (M = Mo, V: L = $PPh_3$, 1/2 phda)을 합성하였다. Acetone 용액에서 이들 중성 화합물과 $AgClO_4$를 반응시켜 [$MCl_3-_nL_2(MeCN)(S)_n$]$(ClO_4)_n$ (n = 1, 2 : s = solvent)의 양이온 화합물을 얻었다. 2가의 양이온 화합물과 중성 화합물을 반응시켜 염소가 다리 연결된 이핵 화합물 $[(MeCN)(L)_2ClM({\mu}-Cl)_2M'Cl(L)_2(MeCN)](ClO_4)_2$ 및 1가의 양이온 화합물과 pyzidine을 2:1 화학양론으로 반응시켜 pyzidine이 다리 결합된 이핵 화합물 $[(MeCN)(L)_2Cl_2M({\mu}-pyz)M'Cl_2(L)_2(MeCN)](ClO_4)_2$형의 호모(M = M'), 헤테로(M ${\neq}$ M') 화합물을 합성하였다. 합성한 착물들은 원소분석과 적외선, $^1H$, $^{13}C$ 핵자기 공명 및 전자 흡수스펙트럼 등을 이용하여 그 특성을 조사하였다.