• Journal of Environmental Health Sciences
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• v.23 no.4
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• pp.91-96
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• 1997

In order to investigate the behavior of particulate carbon and benzo(a)pyrene(B(a)P) in the ambient air, suspended particle matters were collected from April, 1990 to February, 1992, and total carbon(TC), organic carbon(OC), elemental carbon(EC) and B(a)P were measured by an elemental analyzer and a HPLC. The results were as follows the average concentration of TC was 38.6 $\mug/m^3$ and its concentration was higher in winter(45.4 $\mug/m^3$) and fa11(44.3 $\mug/m^3$) than in summer(36.8 $\mug/m^3$) and spring(28.9 $\mug/m^3$). The monthly concentration trends both of EC and OC was similar, but seasonal variation of EC concentrations was larger than that of OC. The average concentration of B (a)P was 2.2 ng/m$^3$, and was higher in winter(4.1 ng/m$^3$) and fall(3.2 ng/m$^3$) than in spring(1.2 ng/m$^3$) and summer(0.6 ng/m$^3$). The seasonal behavior of carbon and B(a)P was to similar except for summer. The correlation coefficient(r) between EC and B(a)P was 0.71, and the correlation coefficient(r) between OC and B(a)P was 0.66.

• Archives of Pharmacal Research
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• v.22 no.2
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• pp.130-136
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• 1999

Mouse guanylate-binding protein 3 (mGBP3) is a 71-kDa GTPase which belongs to GTP-binding protein family. The present study showed that the expression of mGBP3 transcript was readily induced in a dose dependent fashion in the macrophage cell line RAW264.7 treated with either $interferon-{\gamma} (IFN-\gamma)$ or lipopolysaccaride (LPS). The expression of mGBP3 protein was also apparent by 4 and 6 h after the treatment of cells with IFN-\gamma (100 U/ml) or LPS ($1{\mu}g/ml$) , and remained at palteau for at least 24 h. Cycloheximide ($10{\mu}g/ml$) had no effect on the $IFN-\gamma-$ or LPS-induced mGBP3 expression, suggesting that the mGBP3 induction did not require further protein synthesis. Interestingly, a protein kinase C (PKC) inhibitor staurosporine (50 nM) abolished the induction of mGBP3 expression by LPS, but not by $IFN-{\gamma}$. These findings suggest that mGBP3 may be involved in the macrophage activation process and both IFN-\gamma and LS induce the mGBP3 expression through distinct signal transduction pathways.

• Journal of Life Science
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• v.25 no.5
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• pp.487-495
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• 2015

We carried out pH stability, chemical inhibition, chemical modification, and pH-dependent kinetic parameter assessments to further characterize protocatechuate 3,4-dioxygenase from Pseudomonas pseudoalcaligenes KF707. Protocatechuate 3,4-dioxygenase was stable in the pH range of 4.5~10.5. L-ascorbate and glutathione were competitive inhibitors with $K_{is}$ values of 0.17 mM and 0.86 mM, respectively. DL-dithiothreitol was a noncompetitive inhibitor with a $K_{is}$ value of 1.57 mM and a $K_{ii}$ value of 8.08 mM. Potassium cyanide, p-hydroxybenzoate, and sodium azide showed a noncompetitive inhibition pattern with $K_{is}$ values of 55.7 mM, 0.22 mM, and 15.64 mM, and $K_{ii}$ values of 94.1 mM, 8.08 mM, and 662.64 mM, respectively. $FeCl_{2}$ was the best competitive inhibitor with a $K_{is}$ value of $29{\mu}M$. $FeCl_{3}$, $MnCl_{2}$, $CoCl_{2}$, and $AlCl_{3}$ were also competitive inhibitors with $K_{is}$ values of 1.21 mM, 0.85 mM, 3.98 mM, and 0.21 mM, respectively. Other metal ions showed noncompetitive inhibition patterns. The pH-dependent kinetic parameter data showed that there may be at least two catalytic groups with pK values of 6.2 and 9.4 and two binding groups with pK values of 5.5 and 9.0. Lysine, cysteine, tyrosine, carboxyl, and histidine were modified by their own specific chemical modifiers, indicating that they are involved in substrate binding and catalysis.

• The Korean Journal of Mycology
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• v.22 no.2
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• pp.122-129
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• 1994

The role of metal ions for the activity of the mitochondrial $F_1-ATPase$ was studied. Removal of non-heme iron ion from the mitochondria by dialysis against chelating agents, 10 mM ethylenediaminetetraacetic acid(EDTA) and 10 mM o-phenanthroline(o-Phe), led to 56% and 49% inactivation of the enzyme, respectively. The enzyme dialyzed against EDTA was reactivated 81% by the addition of 0.5 mM $Fe^{3+}$ and 70% by 0.5 mM $Mg^{2+}$. But, $Fe^{2+}$ did not reactivate the enzyme. Coexistence of 0.5 mM $Fe^{2+}$ and 0.5 mM $Mg^{2+}$ resulted in 95% reactivation of the enzyme, while $Fe^{3+}$ with 0.5 mM $Mg^{2+}$ did not reactivate the enzyme like the effect of $Fe^{2+}$ alone. The enzyme dialyzed against o-Phe showed the similar results. These data showed that $Fe^{3+}$ is predominantly required for the activity of the mitochondrial $F_1-ATPase$ in Lentinus edodes and stimulated the activity of it by $Mg^{2+}$. $Fe^{3+}$ and $Mg^{2+}$ increased enzyme's affinity for substrate, decreasing the Km value 1.67 mM to 0.65 mM.

• Journal of Preventive Medicine and Public Health
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• v.11 no.1
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• pp.65-75
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• 1978

This study was carried out to investigate air pollution by total suspended particulate(T.S.P.), benzene soluble matter and benzo(a) pyrene in Seoul city. The sampling areas were divided into commercial(Kwang Hwa Moon), indus-trial(Ku Ro Dong) and residensial area(Shin Chon). Sampling was undertaken by High Voulme Air Sampler for four seasons from January 1917 to November 1977. The T.S.P. was extracted with Soxhlet apparatus by benzene and benzo(a) pyrene was separated by column chromatography and thin layer chromatography. The concentrations of benzo(a) pyrene were measured by means of fluorophotometer, and following results were obtained. 1. Arithmetic average concentration for 1-day averaging time of total suspended particulate were $275.6ug/m^3$ in Kwang Hwa Moon, $325.9ug/m^3$ in Ku Ro Dong and $193.0ug/m^3$ in Shin Chon. 2. The seasonal variance of total suspended parti-culate at Ku Ro Dong and Shin Chon were $102.7ug/m^3\;99.6ug/m^3$ respectively and at Kwang Hwa Moon $39.9ug/m^3$. And the concentration of autumn is higher than of that spring at Ku Ro Dong and at Shin Chon, but at Kwang Hwa Moon, the seasonal variance is very little. 3. The concentrations of 50% frequency from geometric mean for 1-day averaging time were $264ug/m^3,\;300ug/m^3\;and\;178ug/m^3$ at Kwang Hwa Moon, Ku Ro Dong and Shin Chon. And geometric standard deviation were 1.27, 1.38 and 1.41 respectively. 4. The concentrations of benzene soluble mater were $26.9ug/m^3$ at Kwang Hwa Moon, $22.7ug/m^3$ at Ku Ro Dong and $15.5ug/m^3$ at Shin Chon, and the ratios to the T.S.P. were 9.8%(range 5.6-14.8%), 7.0%(range 2.4-14.4%) and 8.0%(range 5.5-22.1%) respectively. 5. The concentrations of benzo(a) pyrene were $8.5ug/m^3$ (range $0.8-29.9ug/m^3$) at Kwang Hwa Moon $10.9ug/m^3$(range $1.1-52.0ug/m^3$) at Ku Ro Dong and $5.8ug/m^3$(range $1.5-11.4ug/m^3$) at Shin Chon. 6. The results of this investigation were relatively high in compared with the recommended standards of suspended particulate in air of U.S. Environmental Protection Agency and observed levels of benzo(a)-pyrene in U.S. city.

• Analytical Science and Technology
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• v.25 no.5
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• pp.333-338
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• 2012

IQ motif-containing GTPase-activating proteins (IQGAPs), which are well-known $Ca^{2+}$-independent calmodulin (CaM) binding proteins, are involved in various cellular functions such as cell proliferation, carcinogenesis and cell migration. The IQGAP3 similar to IQGAP1 has four repeated IQ motifs, which are crucial for CaM binding. It has been recently shown that all four IQ motifs of the IQGAP1 could bind to CaM, while not clear the binding of four IQ motifs of the IQGAP3. In this study, we examined the binding between CaM and each IQ motif of IQGAP3. As a result, we found that IQ2 and IQ3, but not IQ1 and IQ4, have a $Ca^{2+}$-independent CaM binding activity. We also found that IQ(3.5-4.4) on the IQGAP3 has $Ca^{2+}$-dependent CaM binding activity as similar with that of IQGAP1. This finding indicates that IQ motifs of the IQGAP3 plays a dynamic role via different interaction of IQ motifs with $Ca^{2+}$/CaM or apoCaM.

• Proceedings of the Korea Water Resources Association Conference
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• 2012.05a
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• pp.368-368
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• 2012

부사호는 당초 유역면적이 $288km^2$로 이 중에서 상류 유역에 건설된 보령댐 유역면적 $163.6km^2$(57%)가 제외됨에 따라 부사호로 유입량이 급격히 줄어들어 약간의 가뭄에도 수질악화가 반복되고 있다. 부사호의 합리적인 용수 수급 관리를 위해 정확한 부사호 유입량의 추정은 절실하다. 부사호 유입량은 보령호 방류량과 부사호 지류 유역 유입량으로 구성되며, 보령호 방류량은 소수력 발전용수, 관개용수, 하천유지용수, 홍수조절 방류량, 월류량으로 구성된다. 부사호 지류 유역으로부터 자연유량에서 하천에서 취수한 공업용수를 공급하고, 웅천읍의 생활용수 $1,164m^3$/일로부터 회귀수가 유입되고, 보령댐 수혜답 1039.5 ha에 관개용수를 공급하고 회귀수가 유입되고, 부사호에서 양수하여 공급한 부사 유역 수혜답 1,141 ha의 회귀수가 유입되고, 6개 저수지 수혜답 396.5 ha의 회귀수가 유입되는 등 지류 유입량의 구성은 매우 복잡하다. 하천에서 취수하여 공급하는 공업용수는 서해화력 5천 $m^3$/일, 보령화력 15천 $m^3$/일로 구성된다. ONE (One parameter New Exponential) 모형을 근간으로 유입량 모형을 구성하였고, 보령댐 자료로 매개변수를 결정하여, 1966~2011년의 부사호 일 유입량을 모의한 결과를 요약하면 다음과 같다. 첫째, 유역면적 $124.4km^2$인 부사호의 지류 유입량과 보령댐 방류량을 고려한 부사호 유입량 자료로부터 유황을 분석한 결과는 연평균하여 풍수량은 2.1 mm/d, $3.083m^3/s$, 평수량은 0.89mm/d, $1.280m^3/s$, 저수량은 0.48 mm/d, $0.695m^3/s$, 갈수량은 0.30 mm/d, $0.428m^3/s$였으며, 연 유입량은 127.23백만 $m^3$에 이르렀다. 둘째, 유역면적 $288km^2$인 보령호 유역의 포함한 부사호의 자연 유입량 자료로부터 즉 보령댐이 없는 경우 유황을 분석한 결과는 연평균하여 풍수량은 1.4 mm/d, $4.599m^3/s$, 평수량은 0.51 mm/d, $1.689m^3/s$, 저수량은 0.20 mm/d, $0.664m^3/s$, 갈수량은 0.06 mm/d, $0.204m^3/s$, 연 유입량은 197.00백만 $m^3$에 이르렀다. 셋째, 보령호가 있는 경우와 없는 경우 유황을 비교하면 있는 경우에서 부사호의 유입량은 고수위의 유량은 감소하고, 저수위의 유량은 증가하는 전형적 상류에 위치한 댐의 저류효과 영향을 여실히 나타내고 있었다. 넷째, 한국하천 유황곡선식에 의한 유역면적 $288km^2$인 부사호 유입량의 풍수량은 1.29 mm/d, $4.292m^3/s$, 평수량은 0.59 mm/d, $1.964m^3/s$, 저수량은 0.33 mm/d, $1.093m^3/s$, 갈수량은 0.13mm/d, $0.424m^3$이르렀다. 결론하면, 보령댐이 있는 경우 연 유입량은 70백만 $m^3$ 감소하였으나, 평갈수기 유입량은 증가하였다.

• Advances in environmental research
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• v.3 no.3
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• pp.207-216
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• 2014

A study was conducted to determine the phytotoxic effect of mercury on seed germination and seedling growth of an important arid legume tree Albizia lebbeck. The seeds germination and seedling growth performance of A. lebbeck responded differently to mercuric chloride treatment (1 mM, 3 mM, 5 mM and 7 mM) as compared to control. Seed germination of A. lebbeck was significantly (p < 0.05) affected by mercury treatment at 1 mM. Root growth of A. lebbeck was not significantly affected by mercury treatment at 1 mM, and 3 mM. Shoot and root length of A. lebbeck were significantly (p < 0.05) affected by 5 mM concentration of mercury treatment. Increase in concentration of mercury treatment at 5 mM and 7 mM significantly (p < 0.05) reduced seedling dry weight of A. lebbeck. The treatment of mercury at 1 mM decreased high percentage of seed germination (22%), seedling length (10%), root length (21.85%) and seedling dry weight (9%). Highest decrease in seed germination (51%), seedling (34%), root length (48%) and seedling dry weight (41%) of A. lebbeck occurred at 7 mM mercury treatment. A. lebbeck showed high percentage of tolerance (78.14%) to mercury at 1 mM. However, 7 mM concentration of mercury produced lowest percentage of tolerance (51.65%) in A. lebbeck. The seed germination potential and seedling vigor index (SVI) clearly decreased with the higher level of mercury. Plantation of A. lebbeck in mercury-polluted area will help in reducing the burden of mercury pollution. A. lebbeck can serve better in coordinating in land management programs in metal contaminated areas. The identification of the toxic concentration of metals and tolerance indices of A. lebbeck would also be helpful for the establishment of air quality standard.

• Korean Journal of Environmental Biology
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• v.21 no.3
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• pp.234-239
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• 2003

3-weeks old Commelina was transferred to and grown in Hoagland solution (($\pm 100 \mu M \;Cd^{2+}, 100 \mu M \;Cd^{2+}+10 \mu M\;IAA,100 \mu M\; Cd^{2+}+100 \mu M \; IAA,100 \mu M \; Cd^{2+} +1 \mu M \; IAA)$) for three weeks and then a number of physiological activities was investigated. In the control the length of stem was increased to 7 cm after 3 weeks, but in the treatment of $Cd^{2+}$, 2.0 cm was grown. In cases of IAA with $Cd^{2+}$, the growth of the plants was increased to 3.7 cm, 5.0 cm and 3.3 cm in 100 $\mu$M, 10 $\mu$M and 1 mM IAA respectively, Cadmium stimulated stomatal opening. The stomata, treated with $Cd^{2+}$ opened to n degree of about 6.1 $\mu\textrm{m}$, but the stomata, treated with no cadmium opened to 4.5 $\mu\textrm{m}$. In the treatment of various concentation of IAA with cadmium the stomata opend to about 1 $\mu\textrm{m}$ more than that of cadmium alone. Cadmium reduced total chlorophyll content up to 13% for 3 weeks. In the treatment of $Cd^{2+}$ ＋ IAA (10 $\mu$M and 100 $\mu$M) the clear change of total chlorophyll content was not observed, but in the addition of 1 mM IAA to $Cd^{2+}$ reduced the total chlorophyll content to about 9%. $Cd^{2+}$ reduced to 9% and 11% of Fv/Fm after two and three weeks respectively. In cases of IAA with $Cd^{2+}$, there were no clear changes of Fv/Fm. Cadmium reduced water potential to 67% after 3 weeks incubation, but in cases of IAA with $Cd^{2+}$, water potential was not clearly changed. Therefore, it could be concluded that the treatment of IAA showed clear alleviation of $Cd^{2+}$-induced several physiological toxicities.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.133-133
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• 2018

The aim of this study was to evaluate the effect of charcoal on germination and early growth of barley sprouts. Five treatments were employed based on different amount and treatment method along with control. Barley seeds were soaked in water for 8 hours. Two types of topping treatment were applied such as, charcoal: 100 g (designated as T1) and charcoal: 200 g (T2). Three kinds of mixing treatment were as follows: barley seeds were mixed with 100g of charcoal (designated as M1), with 200g of charcoal (M2), and with 300g of charcoal(M3). The control did not have any charcoal. In our finding, germination rates were observed 53.3% (control), 26.3%(T1), 36.3%(T2), 67.3%(M1), 81.7%(M2), and 79.7%(M3) at three days after inoculation (DAI). Length of radicle was found at 0.90 cm (control), 0.88 cm (T1), 0.99 cm (T2), 1.03cm (M1), 1.66 cm (M2), and 0.70 cm (M3) in 3 DAI. In addition, sprout length was found 4.5 cm (control), 10.4 cm (T1), 11.9 cm (T2), 5.7 cm (M1), 6.3 cm (M2), and 2.1 cm (M3) in 14 DAI. Fresh weight of sprouts were 0.78g (control), 1.03g (T1), 1.07g (T2), 0.96g (M1), 1.07g (M2), and 0.95g (M3). Among the treatment, topping of seeds on 200g of charcoal (T2) showed longest sprout length and fresh weight. Mixing treatments showed higher germination rates and sprout fresh weight. The results may be attributed to difference in micro-climate conditions (mostly temperature and humidity) in the growth boxes in different treatments.