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Reproductive Ecology and Genetic Variations in the Sea Urchins Anthocidaris crassispina and Hemicentrotus pulcherrimus in the Cheju Coast (제주연안에 서식하는 보라성게 Anthocidaris crassispina와 말똥성게 Hemicentrotus pulcherrimus의 지역별 번식생태학적 특성과 유전적 변이의 비교)

  • 이정재;김범규;강상균;정상철;이기완;최광식
    • Journal of Aquaculture
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    • v.13 no.2
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    • pp.129-135
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    • 2000
  • Genetic variabilities of local populations of Anthocidaris crassispina and Hemicentrotus pulcherrimus were analysed by random amplified polymorphic DNA (RAPD) technique with phenotypic variabilities in timing of gonadal maturation, reproductive output and size of individuals in Cheju. H, pulcherrimus, collected from 4 locations during March 1997, indicated that Daepo individuals were significantly smaller than that at Hamdok, Wimi site A and Wimi site B (ANOVA, p>0.001). Gonodal-somatic index (GSI) of the Wimi site B population was significantly higher than that of three other locations (ANOVA, P>0.0001). An ANOVA test conducted on test size of A. crassispina, harvested from six different locations of Cheju during June 1997, indicated that the size of individuals from Pophwan was significantly smaller (P>0.0001) than that from five other locations. GSIs of urchin m Wimi and Hanlim were significantly higher than that from Pohwan and Oedo (ANOVA, p>0.0001). Genetic similarity, calculated from k13 primer analysis of total DNA, among the six different populations of A. crassispina varied from 0.67 to 0.92, and the values for H. pulcherrimus from 0.60 to 0.73; thus there was no genetic variation among different populations of the same species. Therefore, the populations are genetically homologous and the observed phenotypic variabilities were possibly associated with water temperature and food.

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First Principles Calculations on Magnetism of CrPt3(001) Thin Films (CrPt3(001) 박막의 자성: 제일원리계산)

  • Jeong, Tae Sung;Jekal, Soyoung;Rhim, S.H.;Hong, S.C.
    • Journal of the Korean Magnetics Society
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    • v.27 no.2
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    • pp.41-48
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    • 2017
  • Recent study shows that ordered alloy of $L1_2$ $XPt_3$ (M = V, Cr, Mn, Co, and Fe) exhibits various magnetic phases such as ferromagnetic-to-antiferromagnetic transition at the $MnPt_3$ surface. Moreover, it has been argued that $CrPt_3$, in particular, possess large magnetocrystalline anisotropy and Kerr rotation with possible violation of Hund's rule. As such, we extend our work to thickness dependence of the magnetic structure of $CrPt_3$ thin film using density functional theory. Magnetic ground state of the bulk $CrPt_3$ turns out to be ferromagnetic (FM), where other magnetic phases such as A-type (A-AF), C-type (C-AF), and G-type antiferromagnetic (G-AF) state have higher total energies than FM by 0.517, 0.591, and 0.183 eV, respectively, and magnetic moments of Cr in bulk are respectively 2.807 (FM), 2.805 (A-AF), 2.794 (C-AF) and $2.869_{{\mu}_B}$ (G-AF). We extend our study to $CrPt_3$(001) thin films with CrPt-and Pt-termination. The thickness and surface-termination dependences of magnetism are investigated for 3-9 monolayers (ML), where different magnetic phases from bulk emerge: C-AF for CrPt-terminated 3 ML and G-AF for Pt-terminated 5 ML have energy difference relative to FM by 8 and 54 meV, respectively. Furthermore, thickness- and surface-termination-dependent magnetocrystalline anisotropies of the $CrPt_3$(001) films are discussed.

High Resolution Gravity Mapping and Its Interpretation from both Shipborne and Satellite Gravity Data in the Ulleung Basin (울릉분지에서의 선상중력과 위성중력 통합에 의한 중력 해상도 향상 및 해석)

  • Park, Chan Hong;Kim, Jeong U;Heo, Sik;Won, Jung Seon;Seok, Bong Chul;Yu, Hae Su
    • Journal of the Korean Geophysical Society
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    • v.2 no.1
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    • pp.27-38
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    • 1999
  • The errors between track segments or at the cross-over points of shipborne gravity were successfully reduced by applying a cross-over error adjustment technique using satellite gravity. The integration of shipborne and satellite altimeter-implied free-air gravity anomalies after the cross-over error adjustment resulted in a high resolution gravity map which contains both short and long wavelength components. The successful adjustment of the cross-over errors in the shipborne gravity using the satellite gravity suggests that the shipborne gravity can be combined with the satellite anomalies characterized by a stable and long wavelength component. The resulting free-air anomaly map is evenly harmonized with both short and long wavelength anomalies. Thus the corrected anomaly map can be better used for the geological interpretation. Free-air anomalies with more than 140 mGal in total variations generally correspond to the seafloor topographic changes in their regional patterns. A series of gravity highs are aligned from the Korea Plateau to the Oki Island, which are interpreted to be caused by seamounts or volcanic topographies. The gravity minima along the western and southern shelf edge are associated not only with the local basement morphology and thick sediment fill at the continental margin, but also possibly with the crustal edge effect known for passive continental margins. Series of NE-trending linear anomalies are possibly caused by a swarm of volcanic intrusions followed the initial opening of the Ulleung Basin. The linear high anomalies in the Ulleung Plateau are terminated by the straightly NNW-trending anomalies with a sharp gradient in its western boundary which indicates a fault-line scarp. The opposite side adjoined with the fault-line scarp shows no correlation with the fault-line scarp in geometry indicating that the block might be horizontally slided from the north. A gravity high in contrast to the deepening in seafloor toward the northeastern central Ulleung Basin is probably responsible for the thin crust and shallow seated mantle. The gravity minima along the western and southern shelf edge are associated not only with the local basement morphology and thick sediment fill at the continental margin, but also possibly with the crustal edge effect known for passive continental margins. Series of NE-trending linear anomalies are possibly caused by a swarm of volcanic intrusions followed the initial opening of the Ulleung Basin. The linear high anomalies in the Ulleung Plateau are terminated by the straightly NNW-trending anomalies with a sharp gradient in its western boundary which indicates a fault-line scarp. The opposite side adjoined with the fault-line scarp shows no correlation with the fault-line scarp in geometry indicating that the block might be horizontally slided from the north. A gravity high in contrast to the deepening in seafloor toward the northeastern central Ulleung Basin is probably suggestive of a thin crust and shallow seated mantle.

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Treatment Outcomes and Prognostic Factors in Patients with Multidrug-Resistant Tuberculosis in Korean Private Hospitals (국내 민간병원에서 치료한 다제내성결핵 환자의 치료 결과 및 예후 인자)

  • Park, Jin-Kyeong;Koh, Won-Jung;Kim, Deog-Kyeom;Kim, Eun-Kyung;Kim, Yu-Il;Kim, Hee-Jin;Kim, Tae-Hyung;Kim, Jae-Yeol;Park, Moo-Suk;Park, I-Nae;Park, Jae-Seuk;Lee, Ki-Man;Song, Sook-Hee;Lee, Jin-Hwa;Lee, Seung-Heon;Lee, Hyuk-Pyo;Yim, Jae-Joon;Lim, Jae-Min;JeGal, Yang-Jin;Jung, Ki-Hwan;Huh, Jin-Won;Choi, Jae-Chol;Shim, Tae-Sun
    • Tuberculosis and Respiratory Diseases
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    • v.69 no.2
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    • pp.95-102
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    • 2010
  • Background: The increasing rate of drug-resistant tuberculosis (TB) is a threat to the public health and TB control. In Korea, about 75~80% of TB patients are treated in private hospitals and the rate has been continuously increasing since 2000. Methods: On a retrospective basis, we enrolled 170 newly diagnosed with or retreated for multidrug-resistant TB (MDR-TB) in 2004 from 21 private hospitals. We extracted the following demographics and treatment history from patient medical records: initial treatment outcomes, cumulative survival rates, treatment outcomes, and prognostic factors. Results: Of the 170 patients, the majority were male (64.1%), the mean age was 44.5 years old, and mean body-mass-index was $20.2kg/m^2$. None of the patients tested positive for HIV. Eleven (6.5%) were confirmed to have extensively drug-resistant TB (XDR-TB) at treatment initiation. Treatment success rates were not different between XDR-TB (36.4%, 4/11) and non-XDR MDR-TB (51.6%, 82/159). Default rate was high, 21.8% (37/170). Far advanced disease on X-ray was a significant negative predictor of treatment success; advanced disease and low BMI were risk factors for all-cause mortality. Conclusion: In private hospitals in Korea, the proportion of XDR-TB in MDR-TB was comparable to previous data. The treatment success rate of MDR-/XDR-TB remains poor and the failure rate was quite high. Adequate TB control policies should be strengthened to prevent the further development and spread of MDR-/XDR-TB in Korea.

Ultrastructural Changes on the Cuticular Surface, Excretory and Digestive Organs of Anisakis simplex Larvae Chronologically Recovered from Experimental Cats (실험감염 고양이로부터 경시적으로 회수한 고래회충 유충의 표피, 배설기 및 소화기의 미세구조 변화)

  • Sohn, Woon-Mok
    • Applied Microscopy
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    • v.29 no.2
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    • pp.211-221
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    • 1999
  • The ultrastructural changes of cuticular surface, excretory and digestive organs of Anisakis simplex larvae chronologically recovered from experimental cats were observed with a SEM and TEM. The larva recovered from an experimental cat at 3 days post-infection (PI) retained the cuticular surface with regular transverse striations and a longitudinal groove on the lateral side of body. This finding suggests that the molting of the 3rd stage larva of A. simplex to 4th one occurred from the 3rd day after infection in cats. The excretory organ (renette cell) consisted of a large cell with numerous ductules ramified from the main duct, mitochondria and secretory granules in cytoplasm. Secretory granules in the renette cell of larvae recovered at 24 hours PI were round whereas those of control and larvae recovered at 6 hours PI were amorphous. Muscular esophagus and ventriculus also retained many secretory granules in the cytoplasm. The secretory granules in these organs of larvae recovered at $6\sim24$ hours PI were electron-dense and widely distributed whereas those of control worm were packed in a pocket and retained various electron densities. In the cytoplasm of intestinal epithelial cells, numerous fine glycogen particles and mitochondria were distributed. The chronological changes of secretory granules in renette cell, muscular esophagus and ventriculus seem to be related with the worm penetration into host tissue.

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Changes in Flowering Date and Yielding Characteristics Affected by Transplanting Date in the Early-maturing Rice Cultivar 'Joun' in the Mid-northern Inland of Korea (중북부 중간지대에서 극조생 벼 품종 '조운'의 기계이앙 시기에 따른 개화기 및 수량특성 변화)

  • Yang, Woonho;Kim, Myeong-Ki;Kang, Shingu;Park, Jeong-Hwa;Kim, Sukjin;Choi, Jong-Seo;Yang, Chang-Ihn;Back, Nam-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.4
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    • pp.304-310
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    • 2017
  • This study was conducted over a 3-year period from 2013 to 2015 in the mid-northern inland, Cheolweon, Korea, to investigate changes in flowering date, daily mean temperature during grain filling, and yield characteristics affected by transplanting date in an early-maturing rice variety, 'Joun'. Thirty-day-old seedlings were transplanted at four different dates at 15-day interval from May 5 to June 19. Flowering dates were July 16, July 21, July 31, and August 14 when transplanting was performed on May 5, May 20, June 4, and June 19, respectively. Late transplanting resulted in higher daily mean temperature before flowering but late-transplanted rice required fewer days and lower cumulative temperature to reach flowering from transplanting. As transplanting was delayed, daily mean temperature for 40 days after flowering decreased, whereas daily sunshine hours for the same period increased, with a temperature of $24.8^{\circ}C$ and sunshine for 5.8 hours being recorded at the transplanting on May 5, and with a temperature of $21.0^{\circ}C$ and sunshine for 7.7 hours at the transplanting on June 19. With late transplanting, panicles per square meter significantly decreased, whereas spikelets per panicle showed an increasing trend. Regression analysis showed that maximum head rice yield was attained from the transplanting on May 18, for which the flowering date was July 21, and daily mean temperature for 40 days from that flowering date was $24.6^{\circ}C$. A decrease in head rice yield by 5% and 10% of the maximum was observed for rice transplanted on June 6 and June 15, which resulted in flowering dates of August 2 and August 11, respectively, and the daily mean temperatures for 40 days from flowering were 23.2 and $21.7^{\circ}C$, respectively. Therefore, in mid-northern inland, it is recommended to transplant 'Joun' on May 18 to induce flowering on July 21, when grain filling is subjected to a daily mean temperature of $24.6^{\circ}C$ during active filling stage.

Effects of a Soil-Born Paenibacillus spp. Strain KPB3 on Suppression of Bacterial Wilt Disease Caused by Ralstonia solanacearum (토양에서 분리한 Paenibacillus spp. KPB3의 Ralstonia solanacearum에 의한 세균성 풋마름병 억제 효과)

  • Suk, Jung-Ki;Ipper, Nagesh S.;Lee, Seon-Hwa;Shrestha, Anupama;Park, Duck-Hwan;Cho, Jun-Mo;Hur, Jang-Hyun;Kim, Byung-Sup;Lim, Chun-Keun
    • The Korean Journal of Pesticide Science
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    • v.10 no.4
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    • pp.313-319
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    • 2006
  • Two hundred bacterial strains were isolated from the soil around healthy tomato plants in a polyvinyl house, where most of the other plants showed bacterial wilt symptoms. The strains were screened in vitro for their antibacterial activity. Among them, a strain, KPB3 showed strong bactericidal activity against bacterial wilt pathogen, Ralstonia solanacearum. The strain KPB3 was identified using physiological and biochemical tests, and 16S rRNA analyses. Based on these tests, the strain was found to be closer to genus Paenibacillus. To control the bacterial wilt caused by R. solanacearum, greenhouse experiments were conducted to determine the effectiveness of the Paenibacillus strain KPB3. Drench application of this strain ($4{\times}10^8$ CFU $mL^{-1}$) into the pots containing tomato plants, post-inoculated with the pathogen, R. solanacearum could drastically reduce the disease severity, compared to the non-treated plants. To evaluate effectiveness of this strain under field conditions, experiments were carried out in polyvinyl houses infested with R. solanacearum, during spring and autumn of the year 2006. It was observed that, during spring, bacterial wilt was more prevalent compared to the autumn. During spring, 50.9% disease incidences occurred in non-treated controls, while, Paenibacillus strain KPB3 treated plants showed 24.6% disease incidences. Similarly, during autumn, around 17.2% plants were infected with bacterial wilt in non- treated polyvinyl houses, compared to the Paenibacillus strain KPB3 treated plants, which showed 7.0% disease incidences. These results demonstrated that, Paenibacillus strain KPB3 is a potential biological control agent against bacterial wilt caused by R. solanacearum, effective under greenhouse as well as field conditions. This is the first report showing biocontrol of R. solanacearum using a Paenibacillus spp. under field conditions.

THE ROLE OF TYPE 2 DIABETES AS A PREDISPOSING RISK FACTOR ON THE PULPO-PERIAPICAL PATHOGENESIS: REVIEW ARTICLE (치수 치근단 병소의 전구 위험요인으로서의 제 2 형 당뇨의 역할에 관한 소고)

  • Kim, Jin-Hee;Bae, Kwang-Shik;Seo, Deog-Gyu;Hong, Sung-Tae;Lee, Yoon;Hong, Sam-Pyo;Kum, Kee-Yeon
    • Restorative Dentistry and Endodontics
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    • v.34 no.3
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    • pp.169-176
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    • 2009
  • Diabetes Mellitus (DM) is a syndrome accompanied with the abnormal secretion or function of insulin, a hormone that plays a vital role in controlling the blood glucose level (BGL). Type land 2 DM are most common form and the prevalence of the latter is recently increasing, The aim of this article was to assess whet her Type 2 DM could act as a predisposing risk factor on the pulpo-periapical pathogenesis. Previous literature on the pathologic changes of blood vessels in DM was thoroughly reviewed. Furthermore, a histopathologic analysis of artificially-induced periapical specimens obtained from Type 2 diabetic and DM-resistant rats was compared. Histopathologic results demonstrate that the size of periapical bone destruction w as larger and the degree of pulpal inflammation was more severe in diabetic rats, indicating that Type 2 D M itself can be a predisposing risk factor that makes the host more susceptible to pulpal infection. The possible reasons may be that in diabetic state the lumen of pulpal blood vessels are thickened by atheromatous deposits, and microcirculation is hindered, The function of polymorphonuclear leukocyte is also impair ed and the migration of immune cells is blocked, leading to increased chance of pulpal infection. Also, lack of collateral circulation of pulpal blood vessels makes the pulp more susceptible to infection. These decrease the regeneration capacity of pulpal cells or tissues, delaying the healing process, Therefore, when restorative treatment is needed in Type 2 DM patients, dentists should minimize irritation to the pulpal tissue un der control of BGL.

Susceptibility tests of oral antibiotics including cefixime against Escherichia coli, isolated from pediatric patients with community acquired urinary tract infections (소아 원외 요로감염 환아에서 분리된 E. coli에 대한 cefixime을 포함한 경구 항생제의 감수성 연구)

  • Lee, Soo Young;Lee, Jung Hyun;Kim, Jong Hyun;Hur, Jae Kyun;Kim, Sun Mi;Ma, Sang Hyuk;Kang, Jin Han
    • Clinical and Experimental Pediatrics
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    • v.49 no.7
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    • pp.777-783
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    • 2006
  • Purpose : Urinary tract infection(UTI) is one of the most frequent infections in children. E. coli is the most frequent etiological micropathogen in pediatric community UTI, and E. coli has developed resistance to many antibiotics, highlighting the need for regular surveys of this organism resistant patterns in the community. The aim of this study was to determine the oral antibiotic susceptibility patterns of E. coli, isolated from pediatric patients with uncomplicated community acquired UTI. Methods : E. coli isolates, obtained from pediatric patients with uncomplicated community acquired UTI between October in 2004 to September in 2005. And minimal inhibitory concentrations(MICs) of oral aminopenicillins and beta-lactamase inhibnitors(ampicillin, amoxacillin, ampicillin-sulbactam), oral cephalosporins(cefaclor, cefixime) and sulfa drug(trimethoprime-sulfamethoxazole) were performed according to the National Committee for Clinical Laboratory Standards(NCCLS) guide line. Results : Total 211 organisms were isolated from pediatric out-patients with community UTI. E. coli was the most common organism(89 percent), followed by E. fecalis, Proteus species, S. aureus, M. morganii, and P. aeruginosa. The resistant rates of aminopenicillins and beta-lactamase inhibitors, cefaclor and sulfa drug to E. coli were very high. But, the resistant rate of cefixime was markedly low, and ESBL strains were isolated with small rates. Conclusion : Our study results suggest that aminopenicillins, cefaclor and sulfa drug may not be useful as first line empirical antibiotics to treat pediatric patients with community UTI in Korea. But, 3rd generation cephalosporin such as cefixime can be used as effective second line antibiotics after primary treatment failure, also may be useful as an empirical first line antibiotic. Finally, we conclude that a continuous surveillance study to monitor susceptibility patterns of E. coli in community UTI will be needed for the standard guide lines of empirical oral antibiotic treatment.

Swelling and Mechanical Property Change of Shale and Sandstone in Supercritical CO2 (초임계 CO2에 의한 셰일 및 사암의 물성변화 및 스웰링에 관한 연구)

  • Choi, Chae-Soon;Song, Jae-Joon
    • Tunnel and Underground Space
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    • v.22 no.4
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    • pp.266-275
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    • 2012
  • In this study, a method is devised to implement a supercritical $CO_2$ ($scCO_2$) injection environment on a laboratory scale and to investigate the effects of $scCO_2$ on the properties of rock specimens. Specimens of shale and sandstone normally constituting the cap rock and reservoir rock, respectively, were kept in a laboratory reactor chamber with $scCO_2$ for two weeks. From this stage, a chemical reaction between rock surface and the $scCO_2$ was induced. The effect of saline water was also investigated by comparing three conditions ($scCO_2$-rock, $scCO_2-H_2O$-rock and $scCO_2$-brine(1M)-rock). Finally, we checked the changes in the properties before and after the reaction by destructive and nondestructive testing procedures. The swelling of shale was a main concern in this case. The experimental results suggested that $scCO_2$ has a greater effect on the swelling of the shale than pure water and brine. It was also observed that the largest swelling displacement of shale occurred after a reaction with the $H_2O-scCO_2$ solution. The results of a series of the destructive and nondestructive tests indicate that although each of the property changes of the rock differed depending on the reaction conditions, the $H_2O-scCO_2$ solution had the greatest effect. In this study, shale was highly sensitive to the reaction conditions. These results provide fundamental information pertaining to the stability of $CO_2$ storage sites due to physical and chemical reactions between the rocks in these sites and $scCO_2$.