• Title/Summary/Keyword: M.T.T.F.

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An Experimental Study on the Vibrational Characteristics of the Rotor Blade with Fiber Reinforced Plastics (복합재료 FRP로 제작된 Rotor Blade 진동특성 분석에 관한 실험적 연구)

  • Son, C.Y.;Byun, H.I.;Paik, J.S.;Shin, J.Y.;Lee, J.T.
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.05a
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    • pp.846-851
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    • 2005
  • The purpose of this paper is that investigates the dynamic behavior characteristic of W.T.S(Wind turbine System) and carries out the evaluation analysis during operating W.T.S. To investigate the dynamic behavior characteristic of W.T.S, the experiments to measure vibration of the blade from the attached accelerometer on the flap and edge section of the blade that is one of the most important elements of dynamic characteristic of W.T.S are performed. Natural frequency and mode shape are calculated with commercial program (ANSYS) using the measured vibration acceleration that receives the signal with F.F.T Analyzer from the accelerometer. For validation of these experiments, the finite element analysis is performed with commercial F.E.M program (ANSYS) on the basis of the natural frequency and mode shape. The results indicate that experimental values have good agreements with the finite element analysis.

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C-terminal truncation of a bovine B12 trafficking chaperone enhances the sensitivity of the glutathione-regulated thermostability

  • Jeong, Jinju;Park, Jihyun;Lee, Dong-Yeon;Kim, Jihoe
    • BMB Reports
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    • v.46 no.3
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    • pp.169-174
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    • 2013
  • The human $B_{12}$ trafficking chaperone hCblC is well conserved in mammals and non-mammalian eukaryotes. However, the C-terminal ~40 amino acids of hCblC vary significantly and are predicted to be deleted by alternative splicing of the encoding gene. In this study, we examined the thermostability of the bovine CblC truncated at the C-terminal variable region (t-bCblC) and its regulation by glutathione. t-bCblC is highly thermolabile ($T_m={\sim}42^{\circ}C$) similar to the full-length protein (f-bCblC). However, t-bCblC is stabilized to a greater extent than f-bCblC by binding of reduced glutathione (GSH) with increased sensitivity to GSH. In addition, binding of oxidized glutathione (GSSG) destabilizes t-bCblC to a greater extent and with increased sensitivity as compared to f-bCblC. These results indicate that t-bCblC is a more sensitive form to be regulated by glutathione than the full-length form of the protein.

An Experimental Study on the Vibrational Characteristics of the Rotor Blade with Fiber Reinforced Plastics (복합재료 FRP로 제작된 Rotor Blade 진동특성 분석에 관한 실험적 연구)

  • Paik, J.S.;Lee, K.S.;Park, J.V.;Lee, J.T.;Son, C.Y.
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.15 no.11 s.104
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    • pp.1232-1240
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    • 2005
  • The purpose of this paper is that investigates the dynamic behavior characteristic of W.T.S (wind turbine system) and carries out the evaluation analysis during operating W.T.S. To investigate the dynamic behavior characteristic of W.T.S, the experiments to measure vibration of the blade from the attached accelerometer on the flap and edge section of the blade that is one of the most important elements of dynamic characteristic of W.T.S are performed. Natural frequency and mode shape are calculated with commercial program ( ANSYS) using the measured vibration acceleration that receives the signal with F.F.T Analyzer from the accelerometer For validation of these experiments, the finite element analysis is performed with commercial F.E.M program (ANSYS) on the basis of the natural frequency and mode shape. The results indicate that experimental values have good agreements with the finite element analysis.

Selection of Newly Isolated Mushroom Strains for Tolerance and Biosorption of Zinc In Vitro

  • Gonen Tasdemir, F.;Yamac, M.;Cabuk, A.;Yildiz, Z.
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.483-489
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    • 2008
  • Nine newly isolated mushroom strains were tested to assess both their zinc tolerance and potential for zinc removal from an aqueous solution. Four strains of ectomycorrhizal fungi, namely Clavariadelphus truncatus (T 192), Rhizopogon roseolus (T 21), Lepista nuda (T 373), and Tricholoma equestre (T 174), along with five strains of white rot fungi, Lenzites betulina (S 2), Trametes hirsuta (T 587), Ganoderma spp. (T 99), Polyporus arcularius (T 438), and Ganoderma carnosum (M 88), were investigated using zinc-amended solid and liquid media. Their biosorption properties were also determined. The colony diameter and dry weight were used as tolerance indices for fungal growth. C. truncatus and T. equestre were not strongly inhibited at the highest concentrations of (225 mg/l) zinc in solid media. The most tolerant four strains with solid media, C. truncatus, G carnosum, T. hirsuta, and T. equestre, were then chosen for tolerance tests in liquid media. An ectomycorrhizal strain, C. truncatus, was also detected as the most tolerant strain in liquid media. However, the metal-tolerant strains demonstrated weak activity in the biosorption studies. In contrast, the highest biosorption activity was presented by a more sensitive strain, G. carnosum. In addition, seven different biosorbent types from G. carnosum (M 88) were compared for their Zn (II) biosorption in batch experiments.

DC and RF Characteristics of 100-nm mHEMT Devices Fabricated with a Two-Step Gate Recess (2단계 게이트 리세스 방법으로 제작한 100 nm mHEMT 소자의 DC 및 RF 특성)

  • Yoon, Hyung Sup;Min, Byoung-Gue;Chang, Sung-Jae;Jung, Hyun-Wook;Lee, Jong Min;Kim, Seong-Il;Chang, Woo-Jin;Kang, Dong Min;Lim, Jong Won;Kim, Wansik;Jung, Jooyong;Kim, Jongpil;Seo, Mihui;Kim, Sosu
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.30 no.4
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    • pp.282-285
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    • 2019
  • A 100-nm gate-length metamorphic high electron mobility transistor(mHEMT) with a T-shaped gate was fabricated using a two-step gate recess and characterized for DC and microwave performance. The mHEMT device exhibited DC output characteristics having drain current($I_{dss}$), an extrinsic transconductance($g_m$) of 1,090 mS/mm and a threshold voltage($V_{th}$) of -0.65 V. The $f_T$ and $f_{max}$ obtained for the 100-nm mHEMT device were 190 and 260 GHz, respectively. The developed mHEMT will be applied in fabricating W-band monolithic microwave integrated circuits(MMICs).

Improved Reproductive Efficiency in Gilts by Intrauterine Infusion of Killed Boar Semen before Breeding

  • Capitan, Severino S.;Penalba, F.F.;Geromo, F.B.;Dalumpienes, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.6
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    • pp.789-792
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    • 2006
  • Two separate trials were conducted to determine the effects of uterine priming prior to first breeding and quantify any changes in the reproductive efficiency of gilts. In trial I twelve (12) gilts were randomly assigned to 3 treatments:T1:infusion of distilled water (control), T2: single infusion of killed semen (KS1), and T3: double infusion of killed semen (KS2). Each treatment had 4 breeding gilts which were bred by natural insemination (NI). In trial II, another set of 12 breeding gilts were randomly allotted to the same treatments and were subsequently bred by artificial insemination (AI). Infusions, through the use of AI catheters, were done during the $2^{nd}$ estrous cycle for T1 and T2, whereas infusions for T3 were made during the $1^{st}$ and $2^{nd}$ cycles. Regular breeding was subsequently made during the $3^{rd}$ estrous cycle. All gilts that returned to cycle were rebred within the 30-day period. In trial I (natural breeding), total piglets born was higher (p<0.05) in T2 (12.75 piglets) and T3 (11.75 piglets) than in the control (10.5 piglets). T3 obtained the highest (p<0.05) litter size (10.25 piglets) and heaviest litter weight (74.12 kg) at 28 days weaning, followed by T2 (9.80 piglets and 65.0 kg, respectively). The control yielded the lowest (p<0.05) litter size (7.50) and the lightest litter weight (47.00 kg) at weaning. For Trial II gilts (artificially inseminated), T3 gave higher (p<0.05) litter size born alive (10.88 piglets), total piglets born (11.72 piglets) and live litter weight at birth (15.30 kg) than those of T2 and the control. These results indicate that prebreeding intrauterine infusion of killed boar semen, either single or double, improved the reproductive performance of gilts.

Stock assessment of starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus in the Uljin marine ranching area, Korea (울진 바다목장 강도다리 (Platichthys stellatus)와 넙치 (Paralichthys olivaceus)의 자원평가)

  • Yoon, Sang-Chul;Lee, Sung-Il;Yang, Jae-Hyeong;Yoon, Byoung-Sun;Kim, Jong-Bin;Cha, Hyung-Kee
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.50 no.4
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    • pp.583-594
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    • 2014
  • This study was performed to estimate biomass and provide management guidance through population ecological characteristics, including growth parameters, instantaneous coefficients of natural and fishing mortalities, and age at first capture of the starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus of Korea. For describing growth of this species, a von Bertalanffy growth model was adopted. The von Bertalanffy growth parameters estimated from a non-linear regression for starry flounder were $L_{{\infty}}=48.25cm$, K=0.16/yr, and $t_0=-1.48$, respectively and those for olive flounder were $L_{{\infty}}=86.46cm$, K=0.26/yr, and $t_0=-0.29$, respectively. Biomass of Platichthys stellatus was estimated by direct biomass estimation method was 2.6 M/T, that was estimated by indirect method was 13.4 M/Tt. Those of Paralichthys olivaceus were estimated as 10.1 M/T, 19.3 M/T, respectively. An yield per recruit analysis showed that the current yield per recruit on Platichthys stellatus was about 48.2 g with F=0.646/yr and the age at first capture ($t_c$) 1.35yr, that on Paralichthys olivaceus was about 167.6 g with F=1.121/yr and the age at first capture ($t_c$) 1yr.

Protein Engineering of Flavin-containing Monooxygenase from Corynebacterium glutamicum for Improved Production of Indigo and Indirubin (인디고와 인디루빈의 생산을 증대하기 위한 플라빈-함유 모노옥시게나제의 단백질공학)

  • Jung, Hye Sook;Jung, Hae Bin;Kim, Hee Sook;Kim, Chang Gyeom;Lee, Jin Ho
    • Journal of Life Science
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    • v.28 no.6
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    • pp.656-662
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    • 2018
  • Flavin-containing monooxygenases from Corynebacterium (cFMOs) were mutagenized based on homology modeling to develop variants with an enhanced indigoid production capability. The four mutants, F170Y, A210G, A210S, and T326S, which fused to a maltose-binding protein (MBP), were constructed, and their biochemical properties were characterized. Of these, purified MBP-T326S required a higher concentration of exogenous FAD (100 mM) than the wild-type MBP-cFMO for optimal activity and showed a 3.8-fold increase in the $k_{cat}/K_m$ value at $100{\mu}M$ FAD compared to that of MBP-cFMO at $2{\mu}M$ FAD. The indole oxygenase activities of MBP-T326S decreased to 63-77% compared to that of the MBP-cFMO In addition, MBP-T326S displayed a very low level of futile NADPH oxidase activities (21-24%) in the absence of a substrate. Mutant proteins except for T326S displayed similar $K_m$ and increased $k_{cat}/K_m$ values compared to the wild-type. MBP-F170Y and -A210S mutants showed elevated indole oxygenase activity higher than 3.1- and 2.9-fold, respectively, in comparison with MBP-cFMO. When indigoid production was carried out in LB broth with 2.5 g/l of tryptophan, Escherichia coli expressing cFMO produced 684 mg/l of indigo and 104 mg/l of indirubin, while cells harboring T326S produced 1,040 mg/l of indigo and 112 mg/l of indirubin. The results indicate that the production of indigo was 13% higher when compared to a previous report in which an E. coli expressing FMO from Methylophaga produced 920 mg/l of indigo. The protein engineering of cFMO based on homology modeling provided a more rational strategy for developing indigoid-producing strains.

A NOTE ON THE EXISTENCE OF SOLUTIONS OF HIGHER-ORDER DISCRETE NONLINEAR STURM-LIOUVILLE TYPE BOUNDARY VALUE PROBLEMS

  • Liu, Yuji
    • Journal of applied mathematics & informatics
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    • v.27 no.1_2
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    • pp.205-215
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    • 2009
  • Sufficient conditions for the existence of at least one solution of the boundary value problems for higher order nonlinear difference equations $\{{{{{\Delta^n}x(i-1)=f(i,x(i),{\Delta}x(i),{\cdots},\Delta^{n-2}x(i)),i{\in}[1,T+1],\atop%20{\Delta^m}x(0)=0,m{\in}[0,n-3],}\atop%20\Delta^{n-2}x(0)=\phi(\Delta^{n-1}(0)),}\atop%20\Delta^{n-1}x(T+1)=-\psi(\Delta^{n-2}x(T+1))}\$. are established.

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A study on the structural and electric properties of fluorinated $YBa_2Cu_3O_{7-y}$ (불소화된 $YBa_2Cu_3O_{7-y}$ 초전도체의 구조적, 전기적 성질에 관한 연구)

  • 김재욱;김채옥
    • Electrical & Electronic Materials
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    • v.9 no.4
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    • pp.404-409
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    • 1996
  • The structural and electric properties of $Y_{1-x}$YbF$_{x}$Ba$_{2}$Cu$_{3}$O$_{7-y}$(x=0.0, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6) have been investigated by using XRD(X-ray diffraction), TMA(thennomechanical analysis), NMR(nuclear magnetic resonance) analysis and four probe method. $Y_{1-x}$YbF$_{x}$Ba$_{2}$Cu$_{3}$O$_{7-y}$ samples were prepared by conventional solid-state reaction method using $Y_{2}$O$_{3}$, BaCO$_{3}$, CuO and YbF$_{3}$ power. TMA and high temperature XRD results shows that orthorhombic to tetragonal phase transition occurs in the unfluorinated 1-2-3 sample while the phase change is not observed in the fluorinated 1-2-3 samples. Superconducting transition temperature(T$_{c}$) increases with increasing YbF$_{3}$ content ; T$_{c}$, of the sample reaching maximum of 102K for x=0.3, and then decreases with further increasing YbF$_{3}$ content. The structural analysis and T$_{c}$ results shows that the fluorine doping stabilize the orthorhombic phase, together with the increase in T$_{c}$.}$ c/.TEX> c/.

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