• 한국식품과학회지
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• 제47권1호
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• pp.103-110
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• 2015

본 연구에는 고추씨기름, 녹차씨기름, 들깨기름, 땅콩기름, 목화씨기름, 참깨기름, 호두기름, 홍화씨기름의 fatty acid 구성을 분석하여 oleic acid, linoleic acid 등의 불포화 지방산이 과반이상 함유되어 있음을 확인하였으며 들깨기름은 특이하게 linolenic acid(57.8%)가 과반이상 함유된 것을 확인할 수 있었다. 원재료의 일반성분과 추출한 기름의 품질을 측정하여 식용유 및 튀김용 기름으로 사용하기에 식품공전의 식용유지 및 튀김용유지의 기준 및 규격에 잠재적으로 적합함을 확인하였다. 또한 종실유들의 생리활성을 예측하기위해 세포독성, 지방축적 및 leptin 방출에 미치는 영향을 측정하였다. 그 결과 실험에 사용된 모든 종실유가 0.2 mg/mL 이하에서 독성이 나타나지 않았으며, 고추씨기름(67%, 0.05 mg/mL), 들깨기름(63%, 0.05 mg/mL; 72%, 0.1 mg/mL), 땅콩 기름(58%, 0.05 mg/mL; 68%, 0.1 mg/mL)의 처리가 통계적으로 유의한 수준(p<0.01, p<0.001)에서 3T3-L1 세포내 지방축적을 억제하는 것을 확인할 수 있었고, 고추씨기름(119%, 0.1 mg/mL), 들깨기름(119%, 0.05 mg/mL), 땅콩기름(122%, 0.05 mg/mL; 136%, 0.1 mg/mL), 참깨기름(139%, 0.05 mg/mL; 147%, 0.1 mg/mL), 호두기름(115%, 0.1 mg/mL), 홍화씨기름(114%, 0.1 mg/mL)의 처리가 3T3-L1 세포에서 leptin의 분비를 통계적으로 유의하게(p<0.05, p<0.01, p<0.001) 증가시키는 것이 관찰되었다. 종실유의 정제 및 대량생산 공정개발 등의 향후 연구가 많이 남아있지만 본 연구를 통해 다양한 종류의 종실유들이 식용유소재 및 요리재료로 사용될 수 있는 잠재적 가능성을 가지고 있음을 확인하였으며 유효한 생리활성을 포함하고 있어 기능석 소재로의 개발가치가 높음을 확인할 수 있었다.

• 한국자원식물학회지
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• 제25권6호
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• pp.745-752
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• 2012

Compared to wide ranges of genetic variation of natural populations, very limited Miscanthus cultivar has been released. This study was the first report on the development of Miscanthus cultivar by means of radiation breeding. Seeds of M. sinensis were initially exposed to gamma rays of 250 Gy for 24 h, generated from a $^{60}Co$ gamma-irradiator. The irradiated seeds were sown and then the highly tiller-producing mutants were selected for this study. Biomass-related parameters including tiller number, plant height, stem diameter, and leaf number were measured. Ploidy level and internal transcribed spacer (ITS) were investigated to characterize the mutants compared to wild type (WT) Miscanthus. Plant height and tiller number were negatively related, where multi-tillering mutants were relatively short after 4 month growth. However stem diameter and leaf number were greater in mutants. All the materials used in this study were diploid, implying that the mutants with greater tiller numbers and stem diameter were not likely related to polyploidization. Based on the sequence of ITS regions, the mutants demonstrated base changes from the gamma irradiation where G+C content (%) was decreased in the ITS1, but increased in ITS2 when compared to WT sequence. ITS2 region was more variable than in ITS1 in the mutants, which collectively allows identification of the mutants from WT. Those mutants having enhanced tillers and allelic variations might be used as breeding materials for enhanced biomass-producing Miscanthus cultivars.

• 한국융합학회논문지
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• 제8권12호
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• pp.109-117
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• 2017

본 연구는 지리산 자생식물 19종을 선별하여 항산화 활성 및 항균 효과를 조사하여 천연보존제 융합소재 탐색을 목적으로 하였다. 총폴리페놀은 차나무(87.9 mg GAE/g), 박하(85.1 mg), 연(65.0 mg), 소나무(52.8 mg) 순으로 높은 값을 나타내었다. 총플라보노이드는 연(25.7 mg QUE/g)과 박하(25.4 mg QUE/g)에서 높은 함유량을 나타내었다. $100{\mu}g/mL$ 농도에서 차나무(58.1%), 연(47.9%), 박하(40.6%) 순으로 높은 ABTS radical 소거능을 나타냈으며, DPPH radical 소거능도 비슷한 경향을 나타내었다. 골잎원추리, 솔잎, 산수유 종자 추출물이 E. coli의 증식억제능이 가장 높았고, 할미꽃, 삼백초, 청미래덩굴 추출물은 S. aureus균의 증식을 완전히 억제하여 향후 천연보존 및 방부제 등의 융합소재로 개발가능성을 확인하였다.

• The Korean Journal of Ecology
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• 제16권4호
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• pp.439-457
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• 1993

본 연구는 식물사회학적 방법에 의해 경상북도 안동군 임하댐 유역의 삼림식생을 분류하고 그 환경조건을 해석할 목적으로 실시되었다. 그 결과 다음의 4군락이 식별되었다: 소나무군락, 굴참나무-졸참나무군집, 아까시나무-닭의 장풀군락, 일본잎갈나무군락. 이중에서 소나무군락은 특히 인위적 영향이 미친 정도에 따라 다시 억새조성군, 산거울 조성군, 전형조성군 등 3하위단위로 세분되었다. 이들 삼림군락에 출현한 유관속식물은 총 63과 144속 191종 30변종으로 밝혀졌다. 이 연구에서 식별된 각 군락의 상관적, 종조성적 특징 및 고도, 경사, 지형, 토양과 같은 생육지의 환경조건이 기술되었다. 또, 상관적, 종조성적 특징과 함께 생활형의 분석 및 토양분석을 통하여 조사지에 있어서 일련의 천이경도를 명백히 하였다. 이와 관련한 삼림군락의 천이계열은 아까시나무-？의 장풀군락.일본잎갈나무군락\longrightarrow소나무군락\longrightarrow굴참나무-졸참나무군집\longrightarrow신갈나무군락의 순으로 밝혀졌다. 마지막으로 본 연구를 통해 귀납되는 사항으로서 댐의 건설을 전후한 자연생태계의 보전 방향이 제시되었다.

• The Korean Journal of Ecology
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• 제8권2호
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• pp.109-117
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• 1985

This report is a part of the investigations of the secondary vegetation successions carried out and the analysis of soil properties in the burned areas of forest. The fires-crown fire and surface fire-were occurred at April, 1978 and February, 1984. The investigations were carrited out from August 10. 1983 to September 25, 1984. The burned areas studied are located in southern slope of Mt. Ssalibagu (above sea-level, 590m), So-myon, Sungju-gun, Chollanam-do, Korea. The results are as follows: The floristic compositions of the burned area at the 5th year after the fires were 85 families, 250 genera, 321 species and 53 varieties. Among them, 85 families, 127 genera, 129 species and 30 varieties were found in the currently burned area. In all the sampling sites (10*10cm), 31 and 57 species were found in the currently burned and in the 5th years after the fires, respectively. Miscanthus sinensis var. purpurascens, Quercus serrata and Festuca ovina were dominant species in the both areas. Biological spectra in both the burned areas showed $H-D_1-R_5-e$ type. Degree of the succession(DS) was 412-884 in the 5th years passed burned area and it was high level. Species diversity index(H) was 0.59~1.13 and evennes index(e) was 0.43~0.79, these indexes between both areas were different considerably. Indexes of similarity between both areas were different, too, but that between $B_1$ and $B_6$ was the highest (CCj=0.5). Probably this phenomenon is due to the great numbers of the pine trees appeared simulataneously. Content of the organic matter, N, P, K and Ca of soil in the burned area at the 5th years after the fires was lower than that of soil in the currently burned area.

• Biomolecules & Therapeutics
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• 제19권3호
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• pp.288-295
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• 2011

Amyloid beta ($A{\beta}$)-induced neurotoxicity is a major pathological mechanism of Alzheimer's disease (AD). In this study, we investigated the inhibitory effect of L-theanine, a component of green tea (Camellia sinensis) on $A{\beta}_{1-42}$-induced neurotoxicity and oxidative damages of macromolecules. L-theanine inhibited $A{\beta}_{1-42}$-induced generation of reactive oxygen species, and activation of extracellular signal-regulated kinase and p38 mitogenic activated protein kinase as well as the activity of nuclear factor kappa-B. L-theanine also signifi cantly reduced oxidative protein and lipid damage, and elevated glutathione level. Consistent with the reduced neurotoxic signals, L-theanine (10-50 ${\mu}g$/ml) concomitantly attenuated $A{\beta}_{1-42}$ (5 ${\mu}M$)-induced neurotoxicity in SK-N-MC and SK-N-SH human neuroblastoma cells. These data indicate that L-theanine on $A{\beta}$-induced neurotoxicity prevented oxidative damages of neuronal cells, and may be useful in the prevention and treatment of neurodegenerative disease like AD.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2405-2425
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• 2014

Radiation exposure leads to several pathophysiological conditions, including oxidative damage, inflammation and fibrosis, thereby affecting the survival of organisms. This review explores the radiation countermeasure properties of fourteen (14) plant extracts or plant-derived compounds against these cellular manifestations. It was aimed at evaluating the possible role of plants or its constituents in radiation countermeasure strategy. All the 14 plant extracts or compounds derived from it and considered in this review have shown some radioprotection in different in vivo, ex-vivo and or in vitro models of radiological injury. However, few have demonstrated advantages over the others. C. majus possessing antioxidant, anti-inflammatory and immunomodulatory effects appears to be promising in radioprotection. Its crude extracts as well as various alkaloids and flavonoids derived from it, have shown to enhance survival rate in irradiated mice. Similarly, curcumin with its antioxidant and the ability to ameliorate late effect of radiation exposure, combined with improvement in survival in experimental animal following irradiation, makes it another probable candidate against radiological injury. Furthermore, the extracts of P. hexandrum and P. kurroa in combine treatment regime, M. piperita, E. officinalis, A. sinensis, nutmeg, genistein and ginsan warrants further studies on their radioprotective potentials. However, one that has received a lot of attention is the dietary flaxseed. The scavenging ability against radiation-induced free radicals, prevention of radiation-induced lipid peroxidation, reduction in radiation cachexia, level of inflammatory cytokines and fibrosis, are some of the remarkable characteristics of flaxseed in animal models of radiation injury. While countering the harmful effects of radiation exposure, it has shown its ability to enhance survival rate in experimental animals. Further, flaxseed has been tested and found to be equally effective when administered before or after irradiation, and against low doses (${\leq}5Gy$) to the whole body or high doses (12-13.5 Gy) to the whole thorax. This is particularly relevant since apart from the possibility of using it in pre-conditioning regime in radiotherapy, it could also be used during nuclear plant leakage/accidents and radiological terrorism, which are not pre-determined scenarios. However, considering the infancy of the field of plant-based radioprotectors, all the above-mentioned plant extracts/plant-derived compounds deserves further stringent study in different models of radiation injury.

• Journal of Ecology and Environment
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• 제30권1호
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• pp.17-21
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• 2007

The organic carbon (OC) distribution of Pinus densiflora forest in Songgye valley at Mt. Worak National Park were studied as a part of the National Long-Term Ecological Research in Korea. In order to investigate the OC distribution, OC in plant biomass, litterfall, litter layer on forest floor, and soil were estimated. The density of P. densiflora forest was 1,300 trees/ha, average DBH was $15.2{\pm}6.17\;cm$ and average tree height was $10.7{\pm}2.56\;m$. The shrub layer was dominated by shrubby Quercus variabilis, Fraxinus sieboldiana and lndigofera kirilowii with low frequency, and herb layer was dominated by Pteridium aquilinum and Miscanthus sinensis. Total amount of OC stored in this pine forest was 142.78 ton C/ha. Organic carbon stored in soil and plant biomass accounted for 59.2% and 37.8%, respectively. Amount of OC distributed in trees, shrubs, herbs and litter layer in this pine forest was 51.79, 2.03, 0.12 and 4.29 ton C/ha, respectively. Amount of OC returned to forest floor via litterfall was $1.50\;ton\;C\;ha^{-1}\;yr^{-1}$. Soil organic carbon (SOC) decreased along the soil depth. Total amount of SOC within 50cm soil depth was $84.55\;ton\;C\;ha^{-1}\;50\;cm-depth^{-1}$.

• 환경생물
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• 제22권1호
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• pp.127-132
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• 2004

본 연구는 계룡산 국립공원내 해발 650 m이상의 산림식생을 대상으로 식물사회학적 방법_으로_ 산림군락을 분류하여 계룡산 국립공원 관리에 필요한 기초자료를 제공하고자 수행되었다. 계룡산 상부지역의 총 51개 조사구를 분석한 결과, 산림군락은 신갈나무군락, 서어나무군락, 소나무군락 및 굴참나무군락으로 구분되었다. 신갈나무군락은 29개 조사구에서 나타났으며, 군락 구분종은 신갈나무, 좁은단풍, 천남성, 용수염, 은분취이다. 서어나무군락은 10개 조사구에서 나타났으며, 군락 구분종은 서어나무, 까치박달, 층층나무, 대사초, 산수국이다. 소나무군락은 7개 조사구에서 나타났으며, 군락 구분종은 소나무, 조록싸리, 산딸기, 참억새, 이삭여러, 맑은대쑥, 큰기름새 이다. 굴참나무군락은 5개 조사구에서 나타났으며, 군락 구분종은 굴참나무이다.

• Parasites, Hosts and Diseases
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• 제55권5호
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• pp.575-578
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• 2017

Aspidogastrid trematodes (Subclass Aspidogastrea) are a relatively small group with a characteristic adhesive disc and parasitize in a variety of cold-blooded hosts. Until now, only 2 species in the genus Cotylaspis, i.e., C. coreensis and C. sinensis, have been reported as the aspidogastrid trematode in the Republic of Korea (=Korea). In the present study, we intended to describe a species of aspidogastrids collected from the small intestine of the common carp, Cyprinus carpio, in a faunistic point of view. Total 51 specimens were collected from a carp caught in Nakdong-gang (River) on May 2015. Some of them were prepared as the specimens for light microscopic observations, and some others were prepared for SEM. They were slightly elongated without head lobes, $2,432{\times}840{\mu}m$ in average size, and had characteristic adhesive discs with 4 rows and 46 alveoli in average. The ovary was reniform and was located in the posterior-upper part of the body. The single testis was larger than the ovary and was located below the ovary. The uterus was coiled containing numerous eggs and distributed in the posterior 2/3 of the body. The vitellaria were follicular, and distributed from the mid-level of testis to near the posterior end. The morphological characters with dimensions of our specimens were closely identical with those of Aspidogaster ijimai previously described. A new aspidogastrid is added among the Korean trematode fauna by the present study.