• The Korean Journal of Ecology
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• v.16 no.3
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• pp.353-364
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• 1993

To elucidate ecophysiological factors affecting nutural distribution of evergreen broad-leabed trees in Danohad National Marine Park, water potential, relative water content and photosynthetic activities of 4 species, Cammellia japonica, Machilus thunbergii, Castanopsis cuspidata var.sieboldii and Quercus acuta, were potential began to decrease from 07:00h to its minimum value at 13:00h for C. cuspidata var. sieboldii (-14.3bar) and Q. acuta (-19.4bar) at 16:00 h for M. thunbergii(-17.0bar) and at 19:00 h for C. japonica (-14.5bar), and these showed similar trends to relative water content. Photosynthetic activities of 4 species began to increase from 7:00 h and reached maximum values before their minimum values of water potential occureed. Optimum temperature renges of photosynthetic activities was $18~20^{\circ}C$ for C. japonica - M. thunbergii and $14~16^{\circ}C$ for C. cuspidata var. sieboldii Quercus acuta.Q.acuta had the highest light saturation point of $0.4mM/m^2/s$ and C. japonica the lowest of $0.15mM/m^2/s$. Water potential of 4 species subjected to water stress, began to decrease after 1st day of drought and after 21th day of drought, those of C. japonica, M. thunbergii, C. cuspidata var. sieboldii and Q. acuta decreased to species began to decrease after 7th day of drought and after 21th day, those of M. thunbergii, C. cuspidata var. sieboldii and Q. acuta were dropped to about 50% and C. japonica 83.5%. Photosynthetic activity of 4 specie began to decline in the order of C. cuspidata var. sieboldii, Q.acuta, C. japonica and M. thunbergii after 10th day of drought. These results suggest that the segregated disributions of C. japonica - M. thunbergii on vally positions and C. cuspidata var. sieboldii - Q. acuta on slopes were associated with different responses of water status and photosyntheties to their environment.

• Journal of Ecology and Environment
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• v.45 no.3
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• pp.97-104
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• 2021

Background: Asarum sieboldii Miq., a species of forest understory vegetation, is an herbaceous perennial belonging to the family Aristolochiaceae. The metapopulation of A. sieboldii is distributed sparsely and has a short seed dispersal distance by ants as their seed distributor. It is known that many flowers of A. sieboldii depend on self-fertilization. Because these characteristics can affect negatively in genetic structure, investigating habitat structure and assessment of genetic structure is needed. A total of 27 individuals in a valley were sampled for measuring genetic diversity, genetic distance, and genetic differentiation by RAPDPCR. Results: The habitat areas of A. sieboldii metapopulation were relatively small (3.78~33.60 m²) and population density was very low (five to seven individuals in 20×20 m quadrat). The habitat of A. sieboldii was a very shady (relative light intensity = 0.9%) and mature forest with a high evenness value (J = 0.81~0.99) and a low dominance value (D = 0.19~0.28). The total genetic diversity of A. sieboldii was quite high (h = 0.338, I = 0.506). A total of 33 band loci were observed in five selected primers, and 31 band loci (94%) were polymorphic. However, genetic differentiation along the valley was highly progressed (G_st = 0.548, N_m = 0.412). The average genetic distance between subpopulations was 0.387. The results of AMOVA showed 52.77% of variance occurs among populations, which is evidence of population structuring. Conclusions: It is expected that a small-scale founder effect had occurred, an individual spread far from the original subpopulation formed a new subpopulation. However, geographical distance between individuals would have been far and genetic flow occurred only within each subpopulation because of the low density of population. This made significant genetic distance between the original and new population by distance. Although genetic diversity of A. sieboldii metapopulation is not as low as concerned, the subpopulation of A. sieboldii can disappear by stochastic events due to small subpopulation size and low density of population. To prevent genetic isolation and to enhance the stable population size, conservative efforts such as increasing the size of each subpopulation or the connection between subpopulations are needed.

• Journal of Life Science
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• v.12 no.6
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• pp.803-811
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• 2002

The present study was carried out for research and development of natural antimicrobial from extract of Stachys sieboldii MIQ against food borne bacteria. The hexane extract of Stachys sieboldii MIQ at 250$\mu\textrm{g}$/$m\ell$ per disc showed 15 ~ 20 mm inhibition zone against gram positive and gram negative barteria. Minimum inhibitory concentration (MIC) of hexane extract was 250${\mu}g$/$m\ell$against Bacillus cereus, 250~500${\mu}g$/$m\ell$against Listeria monocytogenes, 500${\mu}g$/$m\ell$ against Staphylococcus aureus, Psedomonas aeruginosa. Observation by transmission electron microscope, showed that disruption of the cell wall assumed to be due to the bactericidal activity. In addition, the membrane integrity of the sensitive cells was disrupted by exposure to Stachys sieboldii MIQ extract on the D-$\beta$-galatosidase activity as substrate of O-nitrophenol-$\beta$-D-galacto-pyranoside. The hexane extract of Stachys sieboldii MIQ was very stable on the pH and thermal stability.

• Horticultural Science & Technology
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• v.17 no.4
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• pp.497-499
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• 1999

Distribution analysis on natural habitats for the genus Calanthe in Mt. Halla of Cheju province revealed that C. discolor and C. sieboldii were found mostly in the range of 120~400 m and 300~600 m above the sea level, respectively. C. bicolor was found in 300~400 m where both C. discolor and C. sieboldii were overlapped. The present morphological assessments showed that C. discolor had the smallest sizes of leaves and floral parts, C. had the largest, while C. bicolor had the intermediate between the above mentioned two species. Flower colors of C. discolor were brownish red and simple white, and that of C. sieboldii was merely golden yellow, whereas C. bicolor had a diverse mixed colors originated from the other two species. The specific distribution of natural habitats and morphological characteristics of three Calanthe species led the suggestion that C. bicolor can be regarded as new species derived originally from natural hybridization between C. discolor and C. sieboldii.

• Korean Journal of Plant Resources
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• v.34 no.5
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• pp.420-432
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• 2021

This study was conducted to evaluate antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity in water extract (SAW) and 60% ethanol extract (SAE) from Stachys sieboldii. As the treatment concentration of each extract S. sieboldii extract increased, antibacterial and antioxidant activity increased. The total polyphenol and total flavonoid contents of SAW were 106.25 ± 0.94 mgGAE/g, 24.4 ± 0.24 mgQE/g and SAE were 124.61 ± 1.11 mgGAE/g, 45.2 ± 3.52 mgQE/g, respectively. The 400 ㎍/mL of SAW and SAE performed more than 53% protective effects against oxidative stress in HepG2 cell lines. All extracts were not showed cytotoxicity to RAW 264.7 cell line at 100 ㎍/mL. NO production was reduced to 44.3 ± 1.4% for SAW and 45.1 ± 1.0% for SAE at a concentration of 100 ㎍/mL. The production of inflammatory cytokines each TNF-α, IL-1β, and IL-6 was inhibited in a concentration-dependent. S. sieboldii extract did not showed Caco-2 cells cytotoxicity and inhibited NO production in concentration-dependent. As the concentration of the S. sieboldii extract increased of α-amylase and protease enzymes activity, which are digestive enzyme. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using S. sieboldii.

• Korean Journal of Pharmacognosy
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• v.44 no.4
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• pp.350-361
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• 2013

Asarum sieboldii has been used for treatment of fever, pain, common cold, and chronic sinusitis in Korea. In this study, we performed quantification analysis of seven major constituents including aristolochic acid I, aristolochic acid II, ${\alpha}$-asarone, ${\beta}$-asarone, elemicin, methyl eugenol, and safrole in the 70% ethanol extract of Asarum sieboldii and its solvent fractions, n-hexane, ethylacetate, n-butanol, and water ones using a ultra-performance liquid chromatography-electrospray ionization-mass spectrometer(UPLC-ESI-MS) and gas chromatography-mass spectrometer(GC-MS). Regression equations of seven components were acquired with $r^2$ values >0.99. The values of limit of detection(LOD) and quantification(LOQ) were 0.1-3.9 ng/mL and 0.3-11.7 mg/mL, respectively. The amount of the seven compounds in Asarum sieboldii were not detected -143.66 mg/g. The established LC-MS/MS and GC-MS methods will be helpful to improve quality control of Asarum sieboldii.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.63-71
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• 2010

This study was carried out to analyze the vegetation and soil characteristic, and ordination between Calanthe discolor and C. sieboldii population, Jeju Island. The C. discolor population was classified into Cryptomeria japonica dominant population, Miscanthus sinensis dominant population, and C. discolor typical population. The C. discolor population was located in elevation of 156m to 430m, and C. sieboldii was located in elevation of 424m to 604m in Jeju Island. In the study sites, soil organic matter, nitrogen, exchangeable potassium, exchangeable calcium, exchangeable magnesium, cation exchange capacity, and soil pH were 30.05~53.58%, 0.74~1.64%, 0.80~1.95cmol$^+$/kg, 4.35~22.53cmol$^+$/kg, 4.00~6.63cmol$^+$/kg, 25.60~51.33cmol$^+$/kg, and 4.83~5.70, respectively. C. discolor population was found in the low elevation and steep sloped area that has less organic matter, total nitrogen, and cation exchange capacity than C. sieboldii population. C. discolor typical population was found in the high elevation area that has more organic matter and total nitrogen in the C. discolor. Miscanthus sinensis dominant population was found in the shade of west direction of japanese black pine. C. sieboldii was found in a high elevation and a gentle sloped area that has high percentage organic matter, total nitrogen, and cation exchange capacity.

• Journal of Life Science
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• v.28 no.7
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• pp.841-848
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• 2018

The flavonoid content and antioxidant effects of extracts from Stachys sieboldii Miq. and Lycopus lucidus Turcz were compared. The flavonoid content of the acetone + methylene chloride (A+M) extract of L. lucidus Turcz was 233.2 mg/g, suggesting that the extract was greater than that of S. sieboldii Miq. In the DPPH assay and the A+M and methanol (MeOH) extracts from L. lucidus Turcz had greater scavenging effects than those of S. sieboldii Miq. (p<0.05). The A+M extract from L. lucidus Turcz (0.5 mg/ml concentration) had an 82% scavenging effect in the DPPH assay. In the ABTS assay, A+M extracts from both S. sieboldii Miq. and L. lucidus Turcz (0.5 mg/ml concentration) had scavenging effects of 90% and 88%, respectively (p<0.05), suggesting that both A+M extracts had greater scavenging effects than those of both MeOH extracts. In a 120 min ROS production assay, all tested extracts dose-dependently decreased the cellular ROS production that was induced by $H_2O_2$, as compared to those produced by exposure to the extract-free control. The A+M extracts from both S. sieboldii Miq. and L. lucidus Turcz had greater inhibitory effects on cellular ROS production than those of both MeOH extracts at all concentrations tested. Treatment with the A+M extracts from S. sieboldii Miq. and L. lucidus Turcz (0.25 mg/ml concentration) inhibited the cellular ROS production by 60% and 86%, respectively. These results suggest that the A+M extracts of Stachys sieboldii Miq. and L. lucidus Turcz inhibit cellular oxidation and may contain valuable bioactive compounds, such as flavonoids.

• Archives of Pharmacal Research
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• v.20 no.3
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• pp.275-279
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• 1997

Helicobacter pylori (H. pylorl) infection is now established as the major pathogenic factor in chronic gastritis and peptic ulcer disease. in addition, there is accumulating evidence that H. pylori plays an important role in the process of gastric carcinogenesis. On the other hand, oriental traditional medicines have been used for stomach disease for thousands of years. In the present study, methanol extract from the stem bark of Magnolia sieboldii (M. sieboldii) and its components were investigated on their inhibitory effects against urease activity and growth of H. pylori in vitro. The methanol extract of M. sieboldii significantly inhibited the growth of H. pylori ATCC 43504 at 5 mg/ml. From the further fractionation, the chloroform fraction inhibited the bacterial growth dose-dependently. Among four fractions separated from the chloroform fraction by silica gel column chromatography, MS-C-2 was the most potent. Costunolide was isolated from the MS-C-2 subtraction by preparative TLC and recrystallization using n-hexane. Anti-H. pylori effect of costunolide was investigated using one commercial strain (H. pylori ATCC 43504) and three clinical strains (H. pylon 4, 43, 82548). Costunolide exhibited potent anti-H. pylori activity, and the MIC was around $100-200{\mu}g/ml$. However, costunolide had no inhibitory effect of H. pylori urease activity at the concentration used for the growth inhibition assay. From these results, we conclude that costunolide inhibits the, growth of H. pylori by the independent manner of H. pylori urease inhibition.

• Journal of Environmental Science International
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• v.23 no.3
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• pp.335-357
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• 2014

This study aims at classifying and interpreting on the vegetation structure and the vegetation transition over 25 years (between 1986 and 2010), and the correlation with the change of some conditions (the vegetation height and coverage on each layer and the climate factors as WI, CI, mean annual temperature, mean annual total precipitation etc.) in the Evergreen Broad-Leaved Forest,, Hong-Do island. The EBLF is classified into five units of vegetation (Hedera rhombea-Machilus thunbergii community (M-M comm.), Castanopsis sieboldii forest (Machilus japonica-Castanopsis sieboldii community; Raphiolepis indica var. umbellata-C. sieboldii community), community (Qa comm.), Carpinus turczaninovii community (Ct comm.), Camellia japonica stand (Cj stand)). The vegetation transition by CCA had high correlation with the height and coverage on each layer and the climate factors, and it did the succession (transition) that the M-M comm. (2010) from Mallotus japonicus community Machilus thunbergii community Carpinus coreana community (Cc comm.) Aucuba japonica community (Aj comm.) Trachelos permum asiaticum var. intermedium-Quercus acuta community (TQ comm.) (1986), the communities of C. sieboldii forest (2010) from Aj comm. TQ comm. Raphiolepis umbellata-Camellia japonica community (RC comm.) (1986), the Qa comm. (2010) from Ardisia japonica-Castanopsis sieboldii community (AC comm.) and TQ comm. (1986), the Ct comm. (2010) from Cc comm. RC comm. Aj comm. Quercus serrata community and the Cj stand (2010) from AC comm. (1986). the height and coverage on each layer are also changed.