• Journal of Physiology & Pathology in Korean Medicine
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• 제19권6호
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• pp.1541-1545
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• 2005

The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Corm fructus. The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on $H_2O_2$-induced cytotoxicity by MTT assay and lipid perixidation by malondialdehyde (MDA) formation, respectively. The results showed that the extract scavenged DPPH radical with the IC50 being $200{\mu}g/mL$. The extract at concentrations of $10-500{\mu}g/ml$ showed dose-dependent in growth of GC-1 cell. $H_2O_2$-induced cytotoxicity (63.0%) was blocked by the extract (10, 50, 100, 250 and $500{\mu}g/ml$) concentration-dependently. Furthermore, the extract (50, 100 and $250{\mu}g/ml$) also displayed a dose-dependent reduction of MDA formation on $H_2O_2$-induced lipid peroxidation. In conclusion, the extract of Corm fructus has potent antioxidant activity.

• Analytical Science and Technology
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• 제18권3호
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• pp.188-193
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• 2005

Three different oriental natural plants (Angelicae Dahuricae Radix, Sanguisorba Officinalis L, Euonymus alatus) were extracted with 70% methanol under refluxing for 4 hr in order to investigate their inhibitory effect on Matrix Metalloproteinase-9 (MMP-9) by a modified gelatin zymography, where only euonymus alatus showed the inhibitory effect on the activity of Matrix MMP-9. The fraction was collected by using the mixture of ethyl acetate and hexane on silica gel column. Seven portions were obtained and three fractions of them (first, third and forth) showed inhibitory effect on the zymography. To verify the effect of these substances on cells, human hepatoma, Hep3B cells as a cancer model, and Chang liver cells as a normal model were selected. In order to examine the cell viability, $1{\mu}g/mL$ of each extract was treated on cells. Most of the methanol soluble fractions showed negligible toxicity on human liver cell line.

• Journal of Physiology & Pathology in Korean Medicine
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• 제20권1호
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• pp.103-114
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• 2006

Herbal mixture water extract of (Chrysanthemum morifolium, Portulaca oleracea, Sanguisorba officinalis, Sophora flavescens, Phellodendron amurense, Cnidium monnieri) which exhibit several beneficial effects including acne and skin diseases, was tested for anti-microbial activity and anti-inflammation effects. The herbal mixture extract showed antimicrobial activity against Stapylococcus epidermis, and Propionibacterium acne. The growth of Stapylococcus epidermis, and Propionibacterium acne was inhibited completely by addition of 1.0% of the extract. Also in the present study we examined the mixture extract on compound 48/80 induced allergy and LPS induced cyclooxygenase-2(COX-2) gene expression in RAW 264.7 macrophage. The results indicated the ear swelling and histamine release induced by compound 48/80 were dose-dependently reduced, ranging 28-60%, and 48-72% , respectively. Furthermore the extract inhibited the expression of LPS-induced COX-2 proteins and mRNAs without an appreciable cytotoxic effects on RAW 264.7 cells. The LPS-induced cytokine gene expression including IL-$1{\beta}$, TNF-$\alpha$, and IL-6 were dose-dependently suppressed by the mixture extract. Based on these results, it is concluded that the herbal mixture water extract can be applied to the acne and skin diseases therapy.

• Journal of Bio-Environment Control
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• 제33권3호
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• pp.163-171
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• 2024

This study was aimed to investigate the effects of layer-by-floor environmental conditions and lower shelf supplemental lighting on the productivity of fresh shoots when growing rosemary in multi-layer cultivation. The 10-cm cuttings from stock plants of common rosemary (Rosemarinus officinalis) were planted in a 128-hole tray, rooted, and then transplanted into pots of 750, 1,300, and 2,000 mL. Afterwards, they were placed on multi-layer shelves (width × length × height: 149 × 60 × 57 cm, 3-layer) in a two-linked greenhouse and cultivated using the sub-irrigation. The productivity of young shoots by layer of the multi-layer shelf was the highest on the third floor (top floor), but productivity decreased sharply after September due to stem lignification caused by excessive light during the summer. Conversely, the lower two layers exhibited faster growth rate of young shoots until the late cultivation period, but the quality decreased due to stem softening and leaf epinasty. To address the excessive light problem on the third floor during the summer, shading was implemented at 30% opacity in July and August, resulting in a 210% increase in rosemary young shoots count and a 162% increase in fresh weight per unit area compared to the unshaded control. To improve the lighting deficiency on the lower layer, supplemental lighting with LED at 30 W increased rosemary young shoot harvest by 168% from June to September compared to no supplemental lighting, but it decreased productivity after September. Therefore, when growing rosemary in multi-layer, it is judged that intensive production of young shoots is possible if the third floor (top layer) is shaded with 30% of light from July to August to prevent stem lignification, and the lower layer is temporarily supplemented with LED 30 W from June to September to increase young shoot growth.

• Korean Journal of Ichthyology
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• 제26권3호
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• pp.171-178
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• 2014

Myogenesis is the formation process of multinucleated myofiber with a contractile capacity from muscle satellite cell (MSCs) during life. This process is tightly controlled by several transcription factors such as Pax3 and Pax7 (paired box protein 3 and 7), MEF2C (myocyte enhancer factor 2) and MRFs (myogenic regulatory factors) etc. On the contrary, myostatin (MSTN) is a transforming growth factor-${\beta}$ superfamily, which functions as a negative regulator of skeletal muscle development and growth. Carnosic acid (CA) is a major phenolic component in rosemary (Rosmarinus officinalis) and have been reported various biological activities such as anticancer, antioxidant, antimicrobial and therapeutic agents for amnesia, dementia, alzheimer's disease. This study was confirmed to effects of CA on muscle cell line and muscle tissue alteration of zebrafish by intramuscular injection or feeding methods. $10{\mu}M$ CA showed a non-cytotoxic on myoblast and a complete inhibition effect against myostatin activity on luciferase assay. In intramuscular injection experiment, the total protein and triglyceride amount of $10{\mu}M/kg$ of CA injected group increased by 11% and decreased by 13% compared to these of the no injected group. In histology analysis of muscle tissues by hematoxylin/eosin staining, the number of muscle fiber of $10{\mu}M/kg$ of CA injected group decreased by 29% and fiber area increased 40% compared to these of no injected group. In feeding experiment, the total protein and triglyceride amount no significance difference compared to these of the normal feeding group. In histology analysis, the number of muscle fiber of 1% CA fed group decreased by 35% and fiber area increased 56% compared to these of normal fed group. We identified that CA have an effect on hypertrophy of muscle fiber in adult zebrafish and the results of this study are considered as the basic data that can reveal the mechanisms of muscle formation via gene and protein level analysis.

• The Korean Journal of Ecology
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• 제26권3호
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• pp.109-114
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• 2003

To investigate ecophysiological characteristics of plants species adapted to moor habitat, we selected 22 species plants and analyzed inorganic cations (K, Ca, Mg), heavy metals (Al, Fe, Mn) and total nitrogen and phosphorus quantitatively. Moojechi moor indicated typical acidic and oligotrophic conditions with pH of 5.0∼5.6 (pH 4.3∼5.1 in soil) and EC of 15∼30μ S/cm, and contained very low contents of soil divalent cation such as Ca and Mg but high contents of heavy metals (esp. Al). With respect to inorganic cation contents, investigated plants species showed remarkable interspecific difference. Plant species belonging to J. effusus var. decipiens, M. japonica, I. globosa, M. sacchariflorus, R. mucronulatum, R. yedoense var. poukhanense, H. micrantha, D. rotundifolia showed very low contents of inorganic cation below 400 μ M/g DW, but plant species of C. palustris var. spontanea, L. sessilifolia, P. mandarinorum, C. lineare, S. austriaca sub. glabra, V. mandshurica, A. decursiva showed high cation contents in leaves. Especially, S. austriaca sub. glabra (Compositae) and V. mandshurica (Violaceae) showed pattern accumulating Ca and Mg with plant growth, but I. ensata var. spontanea (Iridaceae) and S. officinalis (Rosaceae) showed decreasing tendency. Meanwhile, most plant species showed low contents of soluble metal ions in leaves in spite of high heavy metal contents on soil, and indicated remarkable interspecific differences in the total contents and composition of heavy metals accumulated. Despite low contents of N and P on soil, most plant species indicated relatively high contents of N and P in leaves at the early stage of growth, and showed slowly decreasing pattern according to growth. Consequently, it seems that plant species inhabited on Moojechi moor cope with acidic-oligotrophic conditions, accumulating inorganic cations and nitrogen at the early growing stage and reutilizing them in the course of growth, and developing heavy metal excluding mechanism.

• Proceedings of the Plant Resources Society of Korea Conference
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.43-43
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• 2021

로즈마리(Rosmarinus officinalis)는 지중해 지역이 원산이고 꿀풀과에 속하는 다년생 식물로 자생지에서는 식물의 크기가 2m까지 자라는 관목성 식물이다. 식용, 약용, 미용, 향료뿐 아니라 관상용으로도 널리 이용되고 있고 특히 향이 좋아 세계 로즈마리 추출물 시장은 연평균 4.8%씩 증가하여 2027년에는 10억 달러를 넘을 것으로 예측된다. 우리나라도 소비 트렌드 변화에 따른 이용성 확대로 신선허브의 수요가 증가하고 있으나 아직은 허브 식물원료의 대부분을 수입에 의존하고 있고, 로즈마리 역시 식물원료뿐 아니라 가공품까지 외국에서 수입하여 사용하는 실정이다. 2018년 로즈마리 수입량은 신선상태 978kg, 건조상태 23,404kg으로 높은 수입의존에 따른 가격 상승과 긴 유통기간에 의한 품질 저하 등의 문제가 발생하고 있다. 본 연구는 로즈마리 어린 순 재배 적정 삽수 길이를 설정하고 어린 순 생산 가능 기간을 구명하여 추후에 고품질 로즈마리 어린 순 집약생산을 위한 다단재배기술을 확립하고자 수행되었다. 삽수 길이는 5, 10, 15cm로 하였고, 삽목 시기는 4월 하순 ~ 8월 하순까지 30일 간격으로 5회 실시하였다. 적정 삽수 길이 설정 실험에서는 15cm 삽수 발근률이 85.6%로 가장 높았으며 신초 출현시기는 5월 26일, 어린 순 생산시기는 6월 23일로 가장 빨랐고 수확시까지 소요일수는 56일로 가장 짧았다. 기대수량 또한 728g/m²로 가장 높았다. 로즈마리 어린 순 생산 가능 기간 구명 실험에서는 4월 28일 삽목시 발근율이 85.6%로 가장 높았고 육묘기간은 28일 어린 순 생산까지 소요일수는 56일로 가장 짧았다. 삽목 시기별 어린 순 품질 및 생산량은 4월 28일 삽목시 품질이 좋았으며 기대수량 또한 728g/m²로 가장 높았다. 결과적으로 상품성 있는 어린순 생산에 적합한 삽수 길이는 15cm, 삽목 시기는 4월 하순 경에 했을 때, 로즈마리의 생육상태, 수확까지의 기간, 어린 순 생산량 등 종합적인 면에서 가장 우수한 값을 얻을 수 있었다.

• Food Science and Preservation
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• 제20권6호
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• pp.840-845
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• 2013

This study investigated the effects (i.e., the acetylcholinesterase activity, lipid peroxidation, and neuronal survival) of 20 kinds of medicinal water extracts. The water extracts of three medicinal plants - Cornus officinalis, Glycyrrhiza glabra, and Angelica gigas - were found to be the most effective on acetylcholinesterase inhibitory activity. In the lipid peroxidation-generating system induced by $H_2O_2/FeSO_4$ in rat brain homogenates, Perilla frutescens, Polygonum multiflorum, Cinnamomun cassia, and G. glabra exhibited protective activity against lipid peroxidation. The neuronal cell death induced by L-glutamate in PC12 was suppressed by the water extracts of G. glabra, Cinnamomun cassia, Platycodon grandiflorum, and Mentha arvensis at the concentration of $100{\mu}g/mL$. Taken together, these results showed that the water extract of G. glabra has the potential anti-dementia activity, which suggests that it might provide an effective strategy for improving dementia.

• Journal of Physiology & Pathology in Korean Medicine
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• 제27권6호
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• pp.752-758
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• 2013

Estrogen like activities were evaluated using ethanol and hot water extracts of herbal medicines by using an in vitro detection system. Bokryung (Poria cocos), Sanyak (root of Dioscorea batatas) and Mokdanpi (root skin of Paeonia suffruticosa) represented statistically significant estrogen-like activities (p<0.001), while Omija (fruit of Schizandra chinensis), Taeksa (root of Alisma canaliculatum A. BR.), Jihwang (root of Rhemannia glutinosa), and Sansuyu (fruit of Cornus officinalis) did not. Estrogen-like activities of Bokryung hot water extract (500 ${\mu}g/ml$) and ethanol extract (50 ${\mu}g/ml$) were almost same as that of a C M $17{\beta}$-estradiol. Furthermore, estrogen-like activities of ethanol extracts (500 ${\mu}g/ml$) of Bokryung and Mokdanpi were stronger than that of $10^{-7}$ M $17{\beta}$-estradiol. These results suggest that Bokryung, Sanyak and Mokdanpi show estrogen-like activities. Especially, Sanyak and Mokdanpi represented promotive effect on the proliferation of MC3T3-E1 osteoblastic cells. Bokryung, Sanyak and Mokdanpi also exhibited superior inhibitory effect on the viability of RAW 264.7 cells. In conclusion, these three herbal medicines might be interpreted as candidates for the further study or development of functional foods or medicine to prevent or avoid postmenopausal symptoms of women.

• Korean Journal of Environmental Biology
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• 제27권1호
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• pp.114-123
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• 2009

The species composition and biomass of coralline algae around three (Uljin, Wolseong and Gori) nuclear power plants on the east coast of Korea were investigated seasonally from February 1997 to October 2006. As a result, 13 species of coralline algae were found during the past ten years. Among them, Corallina pilulifera, C. officinalis, Amphiroa zonata and Pneophyllum zostericolum were common species that occurred more than 50% in frequency during the study period. Species number of coralline algae were between 10$\sim$12 species at the breakwaters near the outfalls of power plants and 8$\sim$12 species at the control area, and differences in species composition were not observed among study sites. Seasonal fluctuations of mean biomass were 0$\sim$2,530 g dry wt m$^{-2}$ and dominant species in biomass was Corallina pilulifera at all the study sites. The breakwaters of power plants generally had a greater coralline algal biomass than the control area. Biomass proportions of coralline algae at the breakwaters of power plants were also higher than those at the control area. At the Uljin sites, particularly, biomass of coralline algae showed greatest in summer and biomass proportion showed highest during the summer season. Differences in the spatio-temporal patterns of coralline algae around three nuclear power plants on the east coast of Korea were presumably due to the regional temperature variations.