Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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A study on separation and characterization of matrix metalloproteinase-9 inhibitors from natural plants

천연 식물 추출물에서 Matrix Metalloproteinase-9 활성 억제제의 분리 및 특성화에 관한 연구

  • Hur, Yong-Chul (Department of Chemical Engineering, Suwon University) ;
  • Park, Sung-Woo (National Institute of Scientific Investigation) ;
  • Kim, Tai-Jin (Department of Chemical Engineering, Suwon University)
  • Received : 2003.12.24
  • Accepted : 2005.05.02
  • Published : 2005.06.25
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Abstract

Three different oriental natural plants (Angelicae Dahuricae Radix, Sanguisorba Officinalis L, Euonymus alatus) were extracted with 70% methanol under refluxing for 4 hr in order to investigate their inhibitory effect on Matrix Metalloproteinase-9 (MMP-9) by a modified gelatin zymography, where only euonymus alatus showed the inhibitory effect on the activity of Matrix MMP-9. The fraction was collected by using the mixture of ethyl acetate and hexane on silica gel column. Seven portions were obtained and three fractions of them (first, third and forth) showed inhibitory effect on the zymography. To verify the effect of these substances on cells, human hepatoma, Hep3B cells as a cancer model, and Chang liver cells as a normal model were selected. In order to examine the cell viability, $1{\mu}g/mL$ of each extract was treated on cells. Most of the methanol soluble fractions showed negligible toxicity on human liver cell line.

천연 식물 추출물로 처리한 matrix metalloproteinase (MMP)의 저해능 실험에서 귀전우 (Euonymus alatus)의 methanol 가용성 분획이 matrix metalloproteinase (MMP-9) 활성에 저해능이 있는 것으로 나타났다. Silica gel column에서 ethyl acetate와 hexane의 혼합비를 달리하는 방법으로 다섯 개의 부분으로 분획하였고 그 중에서 세 개의 분획이 MMP-9 활성에 저해능이 있는 것으로 나타났다. 인체 간암 세포인 Hep3B 세포주와 인체 정상 세포인 Chang 세포주를 같은 조건으로 키운 후 형태학적 변화를 관찰한 결과, 귀전우의 메탄올 추출물로 처리한 세포에서만 핵의 주위에 검은 반점이 넓게 분포되어 있는 것이 관찰되었다. 인체 정상 세포주에 대한 독성 확인 실험에서 대부분의 메탄올 가용성 분획은 독성을 나타내지 않는 것으로 나타났다.

Keywords

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