• Title/Summary/Keyword: M-continuous

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Fuzzy r-minimal Preopen Sets And Fuzzy r-M Precontinuous Mappings On Fuzzy Minimal Spaces

  • Min, Won-Keun;Kim, Young-Key
    • Journal of the Korean Institute of Intelligent Systems
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    • v.20 no.4
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    • pp.569-573
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    • 2010
  • We introduce the concept of fuzzy r-minimal preopen set on a fuzzy minimal space. We also introduce the concept of fuzzy r-M precontinuous mapping which is a generalization of fuzzy r-M continuous mapping, and investigate characterization of fuzzy r-M precontinuity.

The Development of Serum-Free Media for the Betrovirus Production and Using It in Continuous Production (유전자 재조합된 레트로바이러스의 생산을 위한 무혈청 배지의 개발과 레트로바이러스의 연속 생산공정 개발)

  • 안지수;조수형;김병기
    • KSBB Journal
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    • v.16 no.3
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    • pp.274-280
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    • 2001
  • A serum-free media for CRIP/MFG-LacZ retrovirus production was developed and applied to continuous packed-bed culture system. The serum-free media developed by fractional factorial design contains indulin ($10\mug/mL$), transferrin ($5\mug/mL$), BSA (4 mg/mL), EGF (25 ng/mL), and linoleic acid ($10\mug/mL$). Operation of continuous packed-bed reactor using Fibra-cel enabled the packging cell to stably maintain retrovirus titer for about 1 week. The optimal operation conditions for dilution rate and temperature were 0.67(h(sup)-1) and $32^{circ}C$, respectively. Using this media, the retrovirus titer(cfu/mL) in the packed continuous culture was about 50% that of continuous culture with serum containing DMEM media.

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Decolorization of Dye and Molasses by Continuous and Semi-Continuous Jar-Fermentor Cultures of Geotrichum candidum Dec 1

  • Kim, S.J.;Kim, M.J.;Shoda, M.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.4
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    • pp.306-312
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    • 2006
  • Two culture modes, continuous and semi-continuous, of the decolorization fungus, Geotrichum candidum Dec 1, were compared to obtain a high treatment efficiency of molasses decolorization and a large productivity of peroxidase (DyP) to simultaneously decolorize dyes and molasses. The continuous culture of G. candidum Dec 1 using a 5-I jar-fermentor showed high DyP activity at a low dilution ratio of $0.005h^{-1}$, and decolorization ratio of molasses of 80% was obtained concomitantly. Therefore, a semi-continuous culture was performed by repeated refill and draw. In this mode, approximately 1.5 liters of the culture broth was replaced per cycle when the decolorization ratio of molasses was near 80%. The molasses medium (1.0 liter per day) was treated and the peroxidase productiveity in the drawn culture broth was 26.6U/day, whereas the peroxidase productiveity was 17.9U/day in the continuous culture with a dilution rate of $0.005h^{-1}$. The semi-continuous treatment system was an efficient decolorization method for the strain, G. candidum Dec 1.

Production of Salicylic Acid from Naphthalene by Immobilized Pseudomonas sp. Strain NGK1

  • Shinde, Manohar;Kim, Chi-Kyung;Karegoudar, Timmanagouda-Baramanagouda
    • Journal of Microbiology and Biotechnology
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    • v.9 no.4
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    • pp.482-487
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    • 1999
  • The Pseudomonas sp. strain NGK1 (NCIM 5120) was immobilized in calcium alginate, agar, and polyacrylamide gel matrices. The salicylic acid-producing capacity of freely suspended cells was compared with immobilized cells in batches with a shake culture and continuous culture system in a packed bed reactor. Freely suspended cells ($4\times10^{10}cfu/ml$) produced 12 mM of salicylic acid, whereas cells immobilized in calcium alginate ($1.8\times10^{11}$cfu/g beads), agar ($1.8\times10^{11}$cfu/g beads), and polyacrylamide ($1.6\times10^{11}$cfu/g beads) produced 15, 11, and 16mM of salicylic acid, respectively, from naphthalene at an initial concentration of 25 mM. The continuous production of salicylic acid from naphthalene was investigated in a continuous packed bed reactor with two different cell populations. The longevity of the salicylic acid-producing activity of the immobilized cells from naphthalene was also studied in semi continuous fermentations. The immobilized cells could be reused 18, 13, and more than 20 times without losing salicylic acid-producing activity in calcium alginate-,agar-, and polyacrylamide-entrapped cells, respectively. The study reveals a more efficient utilization of naphthalene and salicylic acid production by the immobilized Pseudomonas sp. strain NGK1 as compared to the free cells.

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Interval- Valued Fuzzy Minimal Structures and Interval-Valued Fuzzy Minimal Spaces

  • Min, Won-Keun
    • International Journal of Fuzzy Logic and Intelligent Systems
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    • v.8 no.3
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    • pp.202-206
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    • 2008
  • We introduce the concept of interval-valued minimal structure which is an extension of the interval-valued fuzzy topology. And we introduce and study the concepts of IVF m-continuous and several types of compactness on the interval-valued fuzzy m-spaces.

Analysis of 32m aerostat gust load using non-linear cable equation (비선형 테더 방정식을 이용한 에어로스탯 돌풍하중해석)

  • Kang, Wang-Gu;Lee, In;Kim, Dong-Min
    • Proceedings of the KSME Conference
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    • 2008.11a
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    • pp.757-761
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    • 2008
  • The aerostat dynamic equation of motion has been built including the tether cable dynamic effects. A numerical program to solve the derived equation of motion has been developed. The dynamic motion of the 32m aerostat has been analyzed under discrete gust and continuous turbulence. The aerostat behaviors under discrete gust which represents a deterministic approach for determining design loads for manned aircraft are solved to verify the effect of aerostat mechanical properties on the aerostat dynamic behavior. Continuous turbulences are simulated for each given altitude, translational mean wind velocity and gust intensity. Dynamic behaviors of the 32m aerostat are simulated for each continuous turbulence conditions. Translational and vertical velocity and pitching behavior and tether reaction force are monitored for each simulation.

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Study on the Continuous Composting Process to Reduce the Use of Bulking Agent in Pig Slurry (톱밥 절감형 돈분 슬러리 연속 퇴비화 공정 연구)

  • Ryoo, J,.W.
    • Journal of Animal Environmental Science
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    • v.13 no.2
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    • pp.121-128
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    • 2007
  • To develop the composting system to reduce the use of bulking agent, continuous composting was performed with farmer scale facility, The plant comprises a horizontal pit reactor closed inside a greenhouse and equipped with a turning machine moving on rails. The pit was 9m wide and 50m long and the maximum height of loaded materials was $1.8m^2$. The materials remained in the reactor for 5 months. During the composting process, temperature and water content measured and water balance was evaluated. The reaction temperature of composting was changed $30{\sim}50^{\circ}C$ and high in the middle and low in under composting piles. The moisture contents of the compost were approximately 70% during the experiment. The amount of effluent was 10.6% and $3.16m^3$ of pig slurry per $1m^3$ of bulking agent was treated during continuous composting process. BOD and SS reduction of the effluent in continuous composting was 86.5% and 92.2%, respectively. Indoor relative humidity in night time was changed between 80 and 100%.

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An Analysis of Bending Behavior of Continuous P.S.C Girder Railway Bridge by Using Down-Up Method (주형의 하강ㆍ상승을 이용한 연속 P.S.C빔 철도교의 휨거동 해석)

  • 구민세;위영민;최인식
    • Proceedings of the KSR Conference
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    • 2001.10a
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    • pp.293-298
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    • 2001
  • 2span continuous Prestressed concrete girder railway bridges, span length 21m, 25m, 30m, 35m, that down-up method is applied and that designed to satisfy service load in accordance with design criteria of railway bridge can be dropped in their hight compared with existing simply supported prestressed concrete girder railway bridges. Continuous bridges result in guaranteeing safety against bending behavior by loading the practical railway moving load with each velocity. But the natural frequency of span length 21m is estimated not to satisfy recommended limitation of UIC 776-1R..

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