• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.135-135
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• 1993

신호전달과정에 중요한 역할을 하고 있는 다기능 serinei/threonine 단백질인산화효소인 protein kinase C(PKC)의 연구를 위해 이 효소의 정제를 뇌에서 착수하였다 PKC의 활성측정을 myelin basic protein을 기질로 하여 20 mM Tris 완충용액 PH 7.5, 0.15 mM [${\gamma}$-$^{32}$P]ATP(3 $\times$ $10^{5}$ cpm), 0.1 mM $Ca^{2＋}$, 10$\mu\textrm{g}$ phosphatidylserine과 2$\mu\textrm{g}$ diolein을 넣어 반응시켰다. 반응은 TCA로 정지시킨 후 방사성 단백질을 Millipore filter paper로 걸러 섬광 계수기로 읽었다. Cytosol PKC의 정제과정은 첫 단계에서 DEAE-cellulose를 사용하였으며, phenyl sepharose CL-4B와 protamine agarose를 연속적으로 이용하여 800배의 정제에 성공했다. SDS-PACE 상에서 80 kD로 나타났으며 순도는 95 % 이상이였다. 이를 이용 PKC의 각종 기질 연구에 착수하기 시작했으며, 이중 MBP의 인산화연구를 통한 myelin의 안정성과 MBP와의 구조 관계가 일부 수행되고 있다 연차적으로 PKC와 이와 관련된 단백질의 특성을 살피기 위해 뇌의 PKC 기질 중 cold stress를 통해 환경에 민감한 것을 찾고 있으며, 현재 autoradiography를 이용해 80 kD, 54 kD, 49 kD와 35 kD의 단백질이 연구대상이 되고있다. 그 중 49 kD는 B-50(또는 GAP43, neuromodulin이라고도 함)일 가능성이 높아 이 단백질 조절과 PKC 활성화 사이의 관계 정립이 흥미로운 과제로 대두되고 있다.다.

• Applied Chemistry for Engineering
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• v.19 no.5
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• pp.519-525
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• 2008

Biodegradation of toluene, styrene and hydrogen sulfide as model compounds of volatile organic compounds and odor from waste gas was investigated experimentally in a biotrickling filter. This study focussed on the description of experimental results with regard to operating conditions. The effect of varying $H_2S$ load rate and inlet concentration was investigated under autotropic and mixotropic environmental conditions. The $H_2S$ removal efficiencies of greater than 99% were achieved at $H_2S$ loads below $10g/m^3{\cdot}hr$ for each environment. It was observed that the maximum elimination capacity of mixotrophic filter was achieved a little greater than the one of autotrophic filter. The biofiltration of toluene and styrene in trickling bed was examined under different gas flow rates, load rates, and inlet concentrations. Below $40g/m^3{\cdot}hr$ of toluene loading, the elimination capacity and loading were identical and it was completely destroyed. In high loading of toluene, the biotrickling filter was operated at its maximum elimination capacity. In the inlet concentration of 0.2, 0.5, and $1.0g/m^3$, the maximum elimination capacity of toluene showed 40, 45, and $60g/m^3{\cdot}hr$, respectively. After a short adaptation period, it was demonstrated that the results of styrene in originally toluene adapted bioreactor was similar with the ones of toluene. However, the performance of filer for styrene is generally a little lower than for toluene. The operating conditions (including liquid flow rate etc.) allowing the highest removal efficiency should be determined experimentally for each specific case.

• Composites Research
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• v.22 no.2
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• pp.43-48
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• 2009

For the practical use of improved interlaminar fracture toughness by interleaving carbon non-woven tissue (CNWT), intelaminar fracture toughnesses of CFRP laminates with CNWT having different weights were experimentally investigated. A suitable weight of interleaved CNWT in CFRP laminates was discussed with Mode I and Mode II tests. Mode I and Mode II interlaminar fracture toughnesses (GIC and GIIC) were obtained by DCB and ENF tests. Six kinds of specimens with CNWT were prepared. The weights of CNWT per square meter for six types of specimens are $8g/m^2,\;10g/m^2,\;12g/m^2,\;16g/m^2,\;20g/m^2$, and $24g/m^2$, respectively. The mean GIC and GIIC values of six kinds of specimens were not substantially different from one another. Compared with the CFRP specimen, the mean GIC values of six kinds of specimens were slightly decreased. But the mean GIIC values increased tremendously at least twice by interleaving CNWT. It seems that there is no interrelationship between the interlaminar fracture toughnesses (GIC and GIIC) and the interleaving CNWT weights. Consequently, it would be desirable to use the CNWT of $8g/m^2$ among the six kinds of CNWTs to take advantage of the interlaminar fracture toughness improved by interleaving CNWT, because the CNWT of $8g/m^2$ is a lightweight and low-priced material.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.36 no.2
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• pp.147-153
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• 1991

In order to clarify the varietal differences of grain shedding in 86 rice varieties with the diverse origin, a bundle of 10 panicles was fallen down on the bottom above 2m height, and several characters related to shattering were investigated. The ratio of grain shedding in the varieties from the Tongil types was the greatest (11. 29%), followed by the native and J aponica types (2.56, 0.17%). The breaking tensile and breaking bending strengths of the primary rachis-branch in the Tongil types were weaker than in the native and Japonica types with mean values of 217g and 20.5g, respectively. The fiber cells length of the primary rachis- branch in the Tongil types were shorter in the native and Japonica types. The ratio of grain shedding was significantly correlated with the breaking tensile and bending strengths of the primary rachis- branch, and fiber cell length.

• Korean Journal of Food Science and Technology
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• v.31 no.3
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• pp.581-585
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• 1999

The antioxidative activity measured by electron donating ability was investigated for the breeding of the highest antioxidative waxy corn (Zea mays L.) and the research for the most effective antioxidant in waxy corn. The electron donating ability was $15.5{\sim}65.0%$. The contents of phenolic compounds and tocopherols and the absorbance at 450 nm were $102.3{\sim}323.5\;{\mu}g/mL$, $15.6{\sim}144.2\;{\mu}g/mL$ and $0.047{\sim}0.206$, respectively. The mean values of electron donating ability and contents of phenolic compounds and tocopherols of four black waxy corn were comparatively high, that is, 48.7%, $267.0\;{\mu}g/mL$ and $87.0\;{\mu}g/mL$, respectively. The electron donating ability was significantly correlated with the level of phenolic compounds and tocopherols but not with the content of carotenoids.

• Korean Journal of Environmental Biology
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• v.19 no.2
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• pp.153-157
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• 2001

The relationship between SPM (Suspended Particulate Matter) and fungal spore in the air was investigated in Seosan, a rural county of Korea, in spring of 2000. SPM concentrations in the air were $199.8\mu{g}\;m^{-3}$, in the 1st Yellow Sand Period (March, 23-24), $249.4\mu{g}\;m^{-3}$ in the 2nd Yellow Sand Period (April, 7-9) and $98.9\mu{g}\;m^{-3}$ in the Non Yellow Sand Period (May, 12-16), respectively. Although there was somewhat difference in total SPM concentration between the two Yellow Sand Periods, majority of the total SPM were composed of $5\mu{m}$ sized coarse particles over the two periods. However, fine particles sized about $1\mu{m}$and coarse particles sized about $5-6\mu{m}$ ultimately showed peaks, which was within typical bimodal pattern at the graph of SPM size distribution in the Non Yellow Sand Period. Four mold genera grown from airborne fungal spores were finally identified in full-grown colonies at the SPM samples during the Yellow Sand Periods. These genera were Fusarium, Aspergillus, Penicillium and Basipetospora.

• Proceedings of the Korean Nuclear Society Conference
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• 1995.05b
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• pp.733-738
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• 1995

$UO_2$ 및 CeO$_2$ 분말을 turbular mixer에서 혼합, 또는 attritor mill에서 분쇄한 후, 각 분말의 특성변화와 이에 따른 소결특성의 변화를 관찰하였다. 혼합된 분말은 평균입자크기와 비표면적이 각각 22.9$\mu\textrm{m}$, 5.1g/㎤이었고, 2시간 분쇄된 분말은 0.5$\mu\textrm{m}$, 6.7g/㎤이었다. 혼합분말로 성형할 경우의 소결밀도는 성형압력의 증가에 관계없이 약 9.6 g/㎤로서 매우 낮았고, 2시간 분쇄된 분말은 10.35 9/㎤ 이상으로 나타나, 혼합핵연료 소결체 제조시 분쇄공정이 반드시 필요함을 나타내었다. 분쇄된 분말을 환원성분위기에서 소결할 경우에는 분쇄시간이 증가함에 따라 결정립이 8$\mu\textrm{m}$까지 증가하였는데 이것은 CeO$_2$ 분말의 미세화와 함께 Ce 성분이 균질 하게 분포되었기 때문으로 사료된다. 그러나 산화성분위기에서는 분쇄시간이 증가할 때 평균 결정립크기는 6$\mu\textrm{m}$로서 변화가 거의 없었다.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2003.11b
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• pp.168-171
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• 2003

화산석/polyurethane 복합담체를 충전한 biofilter에서 ethyl acetate의 제거특성에 관한 연구결과는 다음과 같다. 공탑접촉시간(EBCT)을 30 sec로 운전시에는 ethyl acetate의 유입농도가 300 ppmv 이하에서는 100%의 제거효율을 나타내었다. 유입부하량에 따른 제거용량을 살펴보면 유입부하량이 160 g/m3/hr 이하일때는 유입부햐량과 제거용량과는 직선적인 관계를 보였으며 본 연구에서 ethyl acetate의 최대제거용량은 210 g/m3/hr으로 산정되었다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2021.11a
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• pp.205-205
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• 2021

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.461-465
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• 2007

In this study, changes in the overall antioxidant properties and chemical constituents including total phenolics and vitamin C of R. occidentalis fruit during ripening are studied. The antioxidant activity was measured by the free-radical scavenging activity(DPPH method) and reducing power(potassium ferricyanide method). Although, the weight and diameter of R. occidentalis fruit were increased with the progress of ripening, antioxidant activity and total phenolics were decrease during ripening. The highest free-radical scavenging activity(at $125{\mu}g/m{\ell}$) and reducing power(at $100{\mu}g/m{\ell}$) in fruit were 61.67% and 0.71, respectively. Total phenolic content and vitamin C content in fruit of 5 days after fruit set were $220.73{\mu}g/g$ and $540.45{\mu}g/g$, respectively. A linear correlation(r=0.9761) was shown between free-radical scavenging activity and total phenolic content.